CN100549169C - 酵母中来自Tritirachium album的重组蛋白酶K的表达 - Google Patents
酵母中来自Tritirachium album的重组蛋白酶K的表达 Download PDFInfo
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- CN100549169C CN100549169C CNB028047745A CN02804774A CN100549169C CN 100549169 C CN100549169 C CN 100549169C CN B028047745 A CNB028047745 A CN B028047745A CN 02804774 A CN02804774 A CN 02804774A CN 100549169 C CN100549169 C CN 100549169C
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
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- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Meat, Egg Or Seafood Products (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10105911.6 | 2001-02-09 | ||
| DE10105911A DE10105911A1 (de) | 2001-02-09 | 2001-02-09 | Expression der rekombinanten Proteinase K aus Tritirachium album in Hefe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1592785A CN1592785A (zh) | 2005-03-09 |
| CN100549169C true CN100549169C (zh) | 2009-10-14 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB028047745A Expired - Lifetime CN100549169C (zh) | 2001-02-09 | 2002-02-05 | 酵母中来自Tritirachium album的重组蛋白酶K的表达 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7368274B2 (enExample) |
| EP (1) | EP1360283B1 (enExample) |
| JP (1) | JP2004517643A (enExample) |
| CN (1) | CN100549169C (enExample) |
| AT (1) | ATE353960T1 (enExample) |
| CA (1) | CA2435606C (enExample) |
| CZ (1) | CZ304969B6 (enExample) |
| DE (2) | DE10105911A1 (enExample) |
| DK (1) | DK1360283T3 (enExample) |
| ES (1) | ES2280516T3 (enExample) |
| WO (1) | WO2002064760A2 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7153666B2 (en) | 2003-07-17 | 2006-12-26 | General Atomics | Methods and compositions for determination of glycated proteins |
| JP2007530061A (ja) * | 2004-04-02 | 2007-11-01 | エフ．ホフマン−ラ ロシュ アーゲー | 高度に精製されたプロテナーゼｋ |
| ES2404059T3 (es) | 2006-07-25 | 2013-05-23 | General Atomics | Procedimientos para analizar el porcentaje de hemoglobina glicada |
| US7943385B2 (en) | 2006-07-25 | 2011-05-17 | General Atomics | Methods for assaying percentage of glycated hemoglobin |
| US8673646B2 (en) * | 2008-05-13 | 2014-03-18 | General Atomics | Electrochemical biosensor for direct determination of percentage of glycated hemoglobin |
| CN102839165B (zh) * | 2012-09-26 | 2014-12-10 | 金普诺安生物科技（苏州）有限公司 | 基因突变型重组蛋白酶k及其工业化生产方法 |
| CN104059900A (zh) * | 2013-03-19 | 2014-09-24 | 中国科学院天津工业生物技术研究所 | 一种蛋白酶k及其应用 |
| CN105087529A (zh) * | 2014-05-07 | 2015-11-25 | 天津旭晨科技有限公司 | 基因工程改造蛋白酶k及其生产方法 |
| CN105193640B (zh) * | 2014-06-24 | 2018-10-12 | 金普诺安蛋白质工程技术（北京）有限公司 | 蛋白酶k在皮肤保健和化妆品领域中的应用 |
| CN108450952A (zh) * | 2018-02-08 | 2018-08-28 | 深圳市唯姿贸易有限公司 | 一种细胞修复口服液 |
| CN110106160B (zh) * | 2019-05-29 | 2021-07-20 | 中国农业科学院北京畜牧兽医研究所 | 催化活性提高的碱性蛋白酶突变体prok-g及其编码基因和应用 |
| CN112048518B (zh) * | 2020-08-22 | 2022-06-24 | 江西农业大学 | 一种玉米醇溶蛋白降解酶的制备方法及其应用 |
| CN114181925B (zh) * | 2020-09-14 | 2025-01-17 | 武汉禾元生物科技股份有限公司 | 一种重组蛋白酶k工业化纯化及冻干方法 |
| CN112592931A (zh) * | 2020-12-31 | 2021-04-02 | 安徽丰原发酵技术工程研究有限公司 | 一种生产重组蛋白酶k的方法 |
| CN113337492B (zh) * | 2021-06-02 | 2023-04-07 | 武汉瀚海新酶生物科技有限公司 | 一种蛋白酶k耐热突变体 |
| CN113481225A (zh) * | 2021-07-23 | 2021-10-08 | 武汉瀚海新酶生物科技有限公司 | 一种蛋白酶k高表达工程菌株的构建及应用 |
| CN115011583A (zh) * | 2021-11-26 | 2022-09-06 | 河南合智医药科技有限公司 | 高表达高比活蛋白酶k突变体序列、毕赤酵母表达质粒的构建及菌株筛选和纯化方法 |
| PL244825B1 (pl) | 2021-11-26 | 2024-03-11 | Blirt Spolka Akcyjna | Mutant proteinazy K Tritirachium album i jej zymogenu, plazmid ekspresyjny, rekombinantowy szczep Pichia pastoris i sposób wytwarzania dojrzałej formy mutanta proteinazy K |
| CN115851680A (zh) * | 2022-11-22 | 2023-03-28 | 山东恒鲁生物科技有限公司 | 一种菌的培养物及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3537708A1 (de) | 1985-10-23 | 1987-04-23 | Boehringer Mannheim Gmbh | Verfahren zur aktivierung von t-pa nach expression in prokaryonten |
| AU617996B2 (en) * | 1987-04-03 | 1991-12-12 | Amgen, Inc. | Novel proteolytic enzymes |
| US5191063A (en) | 1989-05-02 | 1993-03-02 | University Of Medicine And Dentistry Of New Jersey | Production of biologically active polypeptides by treatment with an exogenous peptide sequence |
| IL117350A0 (en) | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
-
2001
- 2001-02-09 DE DE10105911A patent/DE10105911A1/de not_active Withdrawn
-
2002
- 2002-02-05 AT AT02712891T patent/ATE353960T1/de active
- 2002-02-05 US US10/468,252 patent/US7368274B2/en not_active Expired - Lifetime
- 2002-02-05 DK DK02712891T patent/DK1360283T3/da active
- 2002-02-05 CN CNB028047745A patent/CN100549169C/zh not_active Expired - Lifetime
- 2002-02-05 CZ CZ2003-2163A patent/CZ304969B6/cs not_active IP Right Cessation
- 2002-02-05 JP JP2002565075A patent/JP2004517643A/ja active Pending
- 2002-02-05 EP EP02712891A patent/EP1360283B1/de not_active Expired - Lifetime
- 2002-02-05 ES ES02712891T patent/ES2280516T3/es not_active Expired - Lifetime
- 2002-02-05 CA CA2435606A patent/CA2435606C/en not_active Expired - Lifetime
- 2002-02-05 DE DE50209478T patent/DE50209478D1/de not_active Expired - Lifetime
- 2002-02-05 WO PCT/EP2002/001144 patent/WO2002064760A2/de not_active Ceased
Non-Patent Citations (2)
| Title |
|---|
| Clonging and expression of the gene encoding a novelproteinase from Tritirachium album limber.. SAMAL B等.ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY,Vol.379 . 1996 * |
| Purification and characterization of a soybean root nodulephosphatase expressed in Pichia pastoris.. PENHEITER ALAN等.Protein expression and purification,Vol.14 No.1. 1998 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002064760A3 (de) | 2002-12-27 |
| CZ20032163A3 (cs) | 2004-05-12 |
| US20040166560A1 (en) | 2004-08-26 |
| US7368274B2 (en) | 2008-05-06 |
| JP2004517643A (ja) | 2004-06-17 |
| ATE353960T1 (de) | 2007-03-15 |
| DE10105911A1 (de) | 2002-08-14 |
| EP1360283B1 (de) | 2007-02-14 |
| CA2435606A1 (en) | 2002-08-22 |
| DE50209478D1 (de) | 2007-03-29 |
| WO2002064760A2 (de) | 2002-08-22 |
| CA2435606C (en) | 2011-07-19 |
| CZ304969B6 (cs) | 2015-02-18 |
| EP1360283A2 (de) | 2003-11-12 |
| CN1592785A (zh) | 2005-03-09 |
| ES2280516T3 (es) | 2007-09-16 |
| DK1360283T3 (da) | 2007-06-11 |
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