JP2008099688A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2008099688A5 JP2008099688A5 JP2007264531A JP2007264531A JP2008099688A5 JP 2008099688 A5 JP2008099688 A5 JP 2008099688A5 JP 2007264531 A JP2007264531 A JP 2007264531A JP 2007264531 A JP2007264531 A JP 2007264531A JP 2008099688 A5 JP2008099688 A5 JP 2008099688A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- phytase
- sequence
- acid sequence
- polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical group 0.000 claims 34
- 108010011619 6-Phytase Proteins 0.000 claims 31
- 229940085127 phytase Drugs 0.000 claims 31
- 108090000765 processed proteins & peptides Proteins 0.000 claims 27
- 229920001184 polypeptide Polymers 0.000 claims 23
- 102000004196 processed proteins & peptides Human genes 0.000 claims 23
- 241000196324 Embryophyta Species 0.000 claims 18
- 102000039446 nucleic acids Human genes 0.000 claims 18
- 108020004707 nucleic acids Proteins 0.000 claims 18
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 16
- 125000003275 alpha amino acid group Chemical group 0.000 claims 16
- 210000004027 cell Anatomy 0.000 claims 15
- 239000002773 nucleotide Substances 0.000 claims 15
- 125000003729 nucleotide group Chemical group 0.000 claims 15
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 241000700605 Viruses Species 0.000 claims 11
- 238000000034 method Methods 0.000 claims 8
- 108010076504 Protein Sorting Signals Proteins 0.000 claims 7
- 210000004507 artificial chromosome Anatomy 0.000 claims 6
- 239000002245 particle Substances 0.000 claims 6
- 150000001413 amino acids Chemical class 0.000 claims 5
- 238000002703 mutagenesis Methods 0.000 claims 5
- 231100000350 mutagenesis Toxicity 0.000 claims 5
- 230000003248 secreting effect Effects 0.000 claims 4
- 108020004705 Codon Proteins 0.000 claims 3
- 210000000056 organ Anatomy 0.000 claims 3
- 102200121588 rs754036398 Human genes 0.000 claims 3
- 230000028327 secretion Effects 0.000 claims 3
- 108700010070 Codon Usage Proteins 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 235000015872 dietary supplement Nutrition 0.000 claims 2
- 229940088598 enzyme Drugs 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000002741 site-directed mutagenesis Methods 0.000 claims 2
- 238000006467 substitution reaction Methods 0.000 claims 2
- 230000008685 targeting Effects 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 108091026890 Coding region Proteins 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 241000235058 Komagataella pastoris Species 0.000 claims 1
- 241000235648 Pichia Species 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- 238000007845 assembly PCR Methods 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 230000004071 biological effect Effects 0.000 claims 1
- 238000012219 cassette mutagenesis Methods 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 239000006052 feed supplement Substances 0.000 claims 1
- 210000004051 gastric juice Anatomy 0.000 claims 1
- 230000001279 glycosylating effect Effects 0.000 claims 1
- 230000013595 glycosylation Effects 0.000 claims 1
- 238000006206 glycosylation reaction Methods 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000003259 recombinant expression Methods 0.000 claims 1
- 230000000306 recurrent effect Effects 0.000 claims 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 claims 1
- 230000003612 virological effect Effects 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/580,515 US6720014B1 (en) | 1997-08-13 | 2000-05-25 | Phytase-containing foodstuffs and methods of making and using them |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001587129A Division JP2003534009A (ja) | 2000-05-25 | 2001-05-24 | 組換え細菌フィターゼおよびその使用 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008099688A JP2008099688A (ja) | 2008-05-01 |
| JP2008099688A5 true JP2008099688A5 (enExample) | 2008-07-03 |
| JP4444324B2 JP4444324B2 (ja) | 2010-03-31 |
Family
ID=24321417
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001587129A Pending JP2003534009A (ja) | 2000-05-25 | 2001-05-24 | 組換え細菌フィターゼおよびその使用 |
| JP2007264531A Expired - Lifetime JP4444324B2 (ja) | 2000-05-25 | 2007-10-10 | 組換え細菌フィターゼおよびその使用 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001587129A Pending JP2003534009A (ja) | 2000-05-25 | 2001-05-24 | 組換え細菌フィターゼおよびその使用 |
Country Status (15)
| Country | Link |
|---|---|
| US (3) | US6720014B1 (enExample) |
| EP (2) | EP1301592B1 (enExample) |
| JP (2) | JP2003534009A (enExample) |
| KR (2) | KR20080013003A (enExample) |
| CN (3) | CN101275124B (enExample) |
| AT (1) | ATE435908T1 (enExample) |
| AU (2) | AU6345601A (enExample) |
| BR (1) | BRPI0111124B1 (enExample) |
| CA (1) | CA2411199C (enExample) |
| DE (1) | DE60139201D1 (enExample) |
| DK (1) | DK1301592T3 (enExample) |
| ES (1) | ES2328670T3 (enExample) |
| IN (1) | IN2002MU01721A (enExample) |
| WO (1) | WO2001090333A2 (enExample) |
| ZA (1) | ZA200209568B (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7078035B2 (en) | 1997-08-13 | 2006-07-18 | Diversa Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
| US7432097B2 (en) | 1997-08-13 | 2008-10-07 | Verenium Corporation | Phytases, nucleic acids encoding them and methods of making and using them |
| US6183740B1 (en) | 1997-08-13 | 2001-02-06 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
| US6855365B2 (en) * | 1997-08-13 | 2005-02-15 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
| US6720014B1 (en) | 1997-08-13 | 2004-04-13 | Diversa Corporation | Phytase-containing foodstuffs and methods of making and using them |
| US6451572B1 (en) * | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| ATE301198T1 (de) * | 1999-03-31 | 2005-08-15 | Cornell Res Foundation Inc | Phospahatasen mit verbesserter phytase-aktivität |
| FR2822381B1 (fr) * | 2001-03-20 | 2004-10-22 | Bio Obtention Sc | Extrait de cucumis melo enrobe et/ou microencapsule dans un agent liposoluble a base de matiere grasse |
| ATE311116T1 (de) * | 2001-08-20 | 2005-12-15 | Leuven K U Res & Dev | Stärkefreie polysaccharide |
| ES2597503T3 (es) * | 2001-10-31 | 2017-01-19 | Huvepharma Eood | Alimento para animales que contiene fitasa y método |
| TWI262083B (en) * | 2001-12-28 | 2006-09-21 | Syngenta Participations Ag | Microbially-expressed thermotolerant phytase for animal feed |
| TW200305430A (en) * | 2001-12-28 | 2003-11-01 | Syngenta Participations Ag | Thermotolerant phytase for animal feed |
| WO2003062409A2 (en) * | 2002-01-25 | 2003-07-31 | Dsm Ip Assets B.V. | Thermostable enzyme compositions |
| DE60335195D1 (de) * | 2002-08-12 | 2011-01-13 | Danisco Us Inc | Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon |
| US7414119B2 (en) | 2002-09-20 | 2008-08-19 | Verenium Corporation | Aldolases, nucleic acids encoding them and methods for making and using them |
| ES2309513T3 (es) * | 2003-05-05 | 2008-12-16 | Kissei Pharmaceutical Co., Ltd. | Uso de derivados de acido fenoxiacetico para tratar vejiga hiperactiva. |
| GB0313964D0 (en) * | 2003-06-16 | 2003-07-23 | Mars Inc | Genotype test |
| AU2004263844A1 (en) * | 2003-07-07 | 2005-02-17 | Syngenta Participations Ag | Reagents, methods and kits for detecting feed enzymes |
| DE102004050410A1 (de) * | 2004-10-15 | 2006-06-08 | Ab Enzymes Gmbh | Polypeptid mit Phytaseaktivität und dieses codierende Nucleotidsequenz |
| GB0423139D0 (en) * | 2004-10-18 | 2004-11-17 | Danisco | Enzymes |
| CN100340662C (zh) * | 2004-11-15 | 2007-10-03 | 刘大庆 | 高比活植酸酶基因及其高效表达 |
| US20070224335A1 (en) * | 2006-03-22 | 2007-09-27 | Shaowen Wu | Stable soy protein beverage composition |
| US7658922B2 (en) | 2005-06-24 | 2010-02-09 | Ab Enzymes Gmbh | Monoclonal antibodies, hybridoma cell lines, methods and kits for detecting phytase |
| GB0600913D0 (en) | 2006-01-17 | 2006-02-22 | Syngenta Ltd | Improvements in or relating to organic compounds |
| DK2365064T3 (en) | 2006-04-04 | 2015-03-30 | Novozymes As | Phytasevarianter |
| WO2008017066A2 (en) * | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| DE102006053059A1 (de) | 2006-11-10 | 2008-05-15 | Ab Enzymes Gmbh | Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz |
| US20080220498A1 (en) * | 2007-03-06 | 2008-09-11 | Cervin Marguerite A | Variant Buttiauxella sp. phytases having altered properties |
| US8143046B2 (en) * | 2007-02-07 | 2012-03-27 | Danisco Us Inc., Genencor Division | Variant Buttiauxella sp. phytases having altered properties |
| US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
| GB0718974D0 (en) | 2007-09-28 | 2007-11-07 | Univ Leuven Kath | oligosaccharides derived from arabinoxylan for prevention of gastrointestinal infection |
| GB0805360D0 (en) * | 2008-03-25 | 2008-04-30 | Univ Leuven Kath | Arabinoxylan oligosaccharide preparation |
| EP2110024A1 (en) * | 2008-04-14 | 2009-10-21 | Institut de Recerca i Tecnologia Agroalimentaires | An enzymatic pre-mixture against Gram negative bacteria colonization in the animal intestinal tract |
| AU2010249500B2 (en) | 2009-05-21 | 2016-03-24 | Basf Enzymes Llc | Phytases, nucleic acids encoding them and methods for making and using them |
| DK2491120T3 (en) * | 2009-10-22 | 2016-03-21 | Basf Se | Synthetic phytasevarianter |
| TW201118170A (en) * | 2009-11-20 | 2011-06-01 | Ind Tech Res Inst | Expression vector for expressing recombinant protein in cyanobacterium |
| US8361956B2 (en) * | 2010-11-18 | 2013-01-29 | Mohammad Ali Malboobi | Recombinant APases nucleic acid sequences |
| CN102559632B (zh) * | 2010-12-22 | 2013-06-26 | 武汉新华扬生物股份有限公司 | 一种在酸性范围内催化活性提高的优化改良的大肠杆菌植酸酶appa-m及其基因和应用 |
| PL2812429T3 (pl) | 2012-02-07 | 2021-01-11 | Danisco Us Inc. | Glikozylacja jako stabilizator dla fitazy |
| IN2015MN00403A (enExample) * | 2012-08-23 | 2015-09-04 | Transalgae Israel Ltd | |
| GB201308828D0 (en) | 2013-03-12 | 2013-07-03 | Verenium Corp | Phytase |
| GB201308843D0 (en) | 2013-03-14 | 2013-07-03 | Verenium Corp | Phytase formulation |
| CN106399273B (zh) * | 2013-07-24 | 2019-09-20 | 东莞泛亚太生物科技有限公司 | 具有提升的酶活性的植酸酶 |
| US10093909B2 (en) | 2013-07-25 | 2018-10-09 | Basf Enzymes Llc | Phytase |
| AU2015279057B2 (en) | 2014-06-27 | 2019-02-28 | Dsm Ip Assets B.V. | A method for improving the nutritional value of animal feed |
| US9605245B1 (en) | 2016-06-30 | 2017-03-28 | Fornia BioSoultions, Inc. | Phytases and uses thereof |
| US10351832B2 (en) | 2016-06-30 | 2019-07-16 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
| US9528096B1 (en) * | 2016-06-30 | 2016-12-27 | Fornia Biosolutions, Inc. | Phytases and uses thereof |
| CN109913401A (zh) * | 2016-09-14 | 2019-06-21 | 四川蓝光英诺生物科技股份有限公司 | 人工组织前体及制备其的方法 |
| CN110117583B (zh) * | 2018-02-05 | 2023-08-04 | 广东溢多利生物科技股份有限公司 | 热稳定和比活提高的植酸酶ecappa突变体及其基因和应用 |
| CN110484520B (zh) * | 2018-05-14 | 2023-08-04 | 广东溢多利生物科技股份有限公司 | 比活提高的植酸酶appa突变体及其基因和应用 |
| CN112640849B (zh) * | 2021-01-13 | 2022-07-08 | 东辽县树安村万力养鸡专业合作社 | 一种东北土鸡反季节产蛋方法 |
| CN114235765A (zh) * | 2021-12-10 | 2022-03-25 | 江西业力医疗器械有限公司 | 一种用于皮肤真菌检测的方法 |
| AU2023404648A1 (en) * | 2022-11-28 | 2025-06-26 | Entozyme Pty Ltd | Transgenic insects and preparations thereof |
| CN119371545B (zh) * | 2024-12-26 | 2025-04-08 | 翌圣生物科技(上海)股份有限公司 | 一种抗植酸酶单克隆抗体或其抗原结合片段、试剂盒及其应用 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340522C (en) | 1987-03-10 | 1999-05-04 | Heinz Dobeli | Fusion proteins containing neighbouring histidines for improved purification |
| DE3725946A1 (de) * | 1987-08-05 | 1989-02-16 | Boehringer Ingelheim Vetmed | Stabilisierter futtermittelzusatz und verfahren zu seiner herstellung |
| US5366736A (en) | 1989-02-16 | 1994-11-22 | University Of Georgia Research Foundation, Inc. | Vitamin D derivative feed compositions and methods of use |
| SK280670B6 (sk) * | 1989-09-27 | 2000-05-16 | Gist-Brocades N.V. | Vyčistená a izolovaná sekvencia dna, konštrukt, ve |
| UA27702C2 (uk) | 1989-09-27 | 2000-10-16 | Гіст-Брокейдс Н.В. | Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger |
| DE69127482T2 (de) | 1990-02-07 | 1998-03-19 | Abbott Lab | Alkalische Phosphatase mit verbesserter spezifischer Aktivität zur Verwendung in Indikatorreagenzien |
| US5593963A (en) | 1990-09-21 | 1997-01-14 | Mogen International | Expression of phytase in plants |
| US5492813A (en) | 1993-11-01 | 1996-02-20 | Boehringer Mannheim Corporation | Muteins of β-galactosidase fragments having increased activity |
| US5830732A (en) * | 1994-07-05 | 1998-11-03 | Mitsui Toatsu Chemicals, Inc. | Phytase |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| KR0169913B1 (ko) | 1996-03-14 | 1999-01-15 | 김은영 | 신균주 바실러스속 ds11과 이로부터 생산되는 신규 파이타아제 |
| WO1997035016A1 (en) | 1996-03-18 | 1997-09-25 | Novo Nordisk Biotech Inc | Polypeptides having phytase activity and nucleic acids encoding same |
| US5939303A (en) | 1996-06-14 | 1999-08-17 | Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada | Phytases of ruminal microorganisms |
| GB2316082A (en) * | 1996-08-13 | 1998-02-18 | Finnfeeds Int Ltd | Phytase |
| US6039942A (en) * | 1996-12-20 | 2000-03-21 | Novo Nordick A/S | Phytase polypeptides |
| KR100206453B1 (ko) | 1997-03-27 | 1999-07-01 | 박원훈 | 신규한플라즈미드를이용하여형질전환시킨균주 이.채씨오엘아이.피와이로부터생산된피타제 |
| NZ330940A (en) | 1997-07-24 | 2000-02-28 | F | Production of consensus phytases from fungal origin using computer programmes |
| US6855365B2 (en) * | 1997-08-13 | 2005-02-15 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
| US6720014B1 (en) | 1997-08-13 | 2004-04-13 | Diversa Corporation | Phytase-containing foodstuffs and methods of making and using them |
| US5876997A (en) * | 1997-08-13 | 1999-03-02 | Diversa Corporation | Phytase |
| US6183740B1 (en) * | 1997-08-13 | 2001-02-06 | Diversa Corporation | Recombinant bacterial phytases and uses thereof |
| KR19990086028A (ko) | 1998-05-25 | 1999-12-15 | 강석진 | 신규한 파이타제 유전자, 재조합 파이타제 및 그 용도 |
| US6451572B1 (en) * | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
| ATE301198T1 (de) * | 1999-03-31 | 2005-08-15 | Cornell Res Foundation Inc | Phospahatasen mit verbesserter phytase-aktivität |
| CN1089368C (zh) * | 1999-04-02 | 2002-08-21 | 中国农业科学院蚕业研究所 | 利用家蚕生物反应器生产植酸酶的方法 |
| EP1170994A1 (en) | 1999-04-23 | 2002-01-16 | University Of Guelph | Transgenic animals expressing salivary proteins |
-
2000
- 2000-05-25 US US09/580,515 patent/US6720014B1/en not_active Expired - Lifetime
-
2001
- 2001-05-24 BR BRPI0111124A patent/BRPI0111124B1/pt not_active IP Right Cessation
- 2001-05-24 KR KR1020077030802A patent/KR20080013003A/ko not_active Ceased
- 2001-05-24 CN CN200810005794.5A patent/CN101275124B/zh not_active Expired - Lifetime
- 2001-05-24 CA CA002411199A patent/CA2411199C/en not_active Expired - Lifetime
- 2001-05-24 AT AT01937752T patent/ATE435908T1/de not_active IP Right Cessation
- 2001-05-24 DK DK01937752T patent/DK1301592T3/da active
- 2001-05-24 EP EP01937752A patent/EP1301592B1/en not_active Expired - Lifetime
- 2001-05-24 KR KR1020027015970A patent/KR100824481B1/ko not_active Expired - Fee Related
- 2001-05-24 WO PCT/US2001/017118 patent/WO2001090333A2/en not_active Ceased
- 2001-05-24 CN CN201410513766.XA patent/CN104313041A/zh active Pending
- 2001-05-24 AU AU6345601A patent/AU6345601A/xx active Pending
- 2001-05-24 DE DE60139201T patent/DE60139201D1/de not_active Expired - Lifetime
- 2001-05-24 CN CN01812774.6A patent/CN1561391B/zh not_active Expired - Lifetime
- 2001-05-24 JP JP2001587129A patent/JP2003534009A/ja active Pending
- 2001-05-24 EP EP07075392A patent/EP1892292A3/en not_active Withdrawn
- 2001-05-24 ES ES01937752T patent/ES2328670T3/es not_active Expired - Lifetime
- 2001-05-24 AU AU2001263456A patent/AU2001263456B2/en not_active Expired
- 2001-12-21 US US10/034,985 patent/US7452706B2/en not_active Expired - Fee Related
-
2002
- 2002-11-25 ZA ZA200209568A patent/ZA200209568B/en unknown
- 2002-12-03 IN IN1721MU2002 patent/IN2002MU01721A/xx unknown
-
2003
- 2003-05-05 US US10/430,356 patent/US7416874B2/en not_active Expired - Fee Related
-
2007
- 2007-10-10 JP JP2007264531A patent/JP4444324B2/ja not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2008099688A5 (enExample) | ||
| CN102239183B (zh) | 大量分泌的蛋白的筛选和它们作为融合配偶体在重组蛋白制备中应用 | |
| CA2411199A1 (en) | Recombinant bacterial phytases and uses thereof | |
| JP2010517532A5 (enExample) | ||
| CA2345905A1 (en) | Plant-optimized polynucleotides encoding approximately 15 kda and approximately 45 kda pesticidal proteins | |
| KR20080042823A (ko) | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 | |
| KR102254411B1 (ko) | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 | |
| CN105296454A (zh) | 一种褐藻胶裂解酶基因algp及其制备方法与表达 | |
| CN104928310B (zh) | 甲硫氨酸裂解酶及其编码基因和生物合成方法 | |
| CN105713883B (zh) | 一种l-脯氨酸-4-羟基化酶及其应用 | |
| CN107936096A (zh) | 一种能有效提高蛋白分泌效率的信号肽及其应用 | |
| CN109182304B (zh) | 一种α-淀粉酶基因及其应用 | |
| CN106701813A (zh) | 表达载体及其构建方法与应用 | |
| CN107674119A (zh) | 一种枯草芽孢杆菌可有效提高分泌的信号肽及其应用 | |
| CN108531527A (zh) | 一种d-阿洛酮糖-3-差向异构酶的应用 | |
| CN113388591A (zh) | 漆酶及其突变体和应用 | |
| CN1444655A (zh) | 在原核宿主细胞中制备重组蛋白 | |
| CN102747060A (zh) | D-氨甲酰水解酶的突变体及其制备方法和应用 | |
| MX2011007492A (es) | Proteinas de fusion, proceso de preparacion y uso de estas en sistemas de expresion de proteinas recombinantes. | |
| JP2007517519A5 (ja) | 難発現性タンパク質の分泌のためのタンパク質融合因子(tfp)を明らかにする方法、タンパク質融合因子(tfp)ライブラリーを製造する方法、及び難発現性タンパク質の組み換え的生産方法 | |
| CN103255156A (zh) | 位点突变的高活热稳定性纳豆激酶基因及其应用 | |
| CN115197928B (zh) | 天冬氨酸酶突变体及其制备方法和用途 | |
| CN100575492C (zh) | 一种编码双齿围沙蚕蛋白酶的cDNA序列及其氨基酸序列 | |
| JPH07313159A (ja) | クロストリジウム ヒストリチカムからの組換えタイプiiコラゲナーゼ及び細胞及び細胞群の単離のためへのその使用 | |
| CN113025595A (zh) | 耐酸脂肪酶 |