JP2004501615A5 - - Google Patents
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- Publication number
- JP2004501615A5 JP2004501615A5 JP2001576947A JP2001576947A JP2004501615A5 JP 2004501615 A5 JP2004501615 A5 JP 2004501615A5 JP 2001576947 A JP2001576947 A JP 2001576947A JP 2001576947 A JP2001576947 A JP 2001576947A JP 2004501615 A5 JP2004501615 A5 JP 2004501615A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- nucleic acid
- pair
- region
- branching region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 239000000523 sample Substances 0.000 claims 82
- 150000001875 compounds Chemical class 0.000 claims 23
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 15
- 238000000034 method Methods 0.000 claims 15
- 239000002853 nucleic acid probe Substances 0.000 claims 15
- 238000004132 cross linking Methods 0.000 claims 12
- 150000007523 nucleic acids Chemical group 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 8
- 239000000376 reactant Substances 0.000 claims 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- BGEBZHIAGXMEMV-UHFFFAOYSA-N 5-methoxypsoralen Chemical compound O1C(=O)C=CC2=C1C=C1OC=CC1=C2OC BGEBZHIAGXMEMV-UHFFFAOYSA-N 0.000 claims 4
- 239000000427 antigen Substances 0.000 claims 4
- 102000036639 antigens Human genes 0.000 claims 4
- 108091007433 antigens Proteins 0.000 claims 4
- -1 aryl olefins Chemical class 0.000 claims 4
- SQBBOVROCFXYBN-UHFFFAOYSA-N methoxypsoralen Natural products C1=C2OC(=O)C(OC)=CC2=CC2=C1OC=C2 SQBBOVROCFXYBN-UHFFFAOYSA-N 0.000 claims 4
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 claims 4
- ZXLJWZULPBXHSY-UHFFFAOYSA-N pyrano[2,3-g]chromene-2,7-dione Chemical compound O1C(=O)C=CC2=C1C=C1C=CC(=O)OC1=C2 ZXLJWZULPBXHSY-UHFFFAOYSA-N 0.000 claims 4
- PMLMHGKSGPOBAX-UHFFFAOYSA-N pyrano[3,2-g]chromene-2,8-dione Chemical compound C1=CC(=O)OC2=C1C=C1C=CC(=O)OC1=C2 PMLMHGKSGPOBAX-UHFFFAOYSA-N 0.000 claims 4
- 150000003230 pyrimidines Chemical class 0.000 claims 4
- 125000006853 reporter group Chemical group 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 claims 3
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 claims 2
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 claims 2
- DWAOUXYZOSPAOH-UHFFFAOYSA-N 4-[2-(diethylamino)ethoxy]furo[3,2-g]chromen-7-one;hydrochloride Chemical compound [Cl-].O1C(=O)C=CC2=C1C=C1OC=CC1=C2OCC[NH+](CC)CC DWAOUXYZOSPAOH-UHFFFAOYSA-N 0.000 claims 2
- LDRUKBXKOFTVNA-UHFFFAOYSA-N 7-(2,3-dihydroxypropoxy)chromen-2-one Chemical compound C1=CC(=O)OC2=CC(OCC(O)CO)=CC=C21 LDRUKBXKOFTVNA-UHFFFAOYSA-N 0.000 claims 2
- CFNMUZCFSDMZPQ-GHXNOFRVSA-N 7-[(z)-3-methyl-4-(4-methyl-5-oxo-2h-furan-2-yl)but-2-enoxy]chromen-2-one Chemical compound C=1C=C2C=CC(=O)OC2=CC=1OC/C=C(/C)CC1OC(=O)C(C)=C1 CFNMUZCFSDMZPQ-GHXNOFRVSA-N 0.000 claims 2
- BUNGCZLFHHXKBX-UHFFFAOYSA-N 8-methoxypsoralen Natural products C1=CC(=O)OC2=C1C=C1CCOC1=C2OC BUNGCZLFHHXKBX-UHFFFAOYSA-N 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- QXKHYNVANLEOEG-UHFFFAOYSA-N Methoxsalen Chemical compound C1=CC(=O)OC2=C1C=C1C=COC1=C2OC QXKHYNVANLEOEG-UHFFFAOYSA-N 0.000 claims 2
- 229960002045 bergapten Drugs 0.000 claims 2
- KGZDKFWCIPZMRK-UHFFFAOYSA-N bergapten Natural products COC1C2=C(Cc3ccoc13)C=CC(=O)O2 KGZDKFWCIPZMRK-UHFFFAOYSA-N 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 230000015556 catabolic process Effects 0.000 claims 2
- 238000006243 chemical reaction Methods 0.000 claims 2
- 125000004122 cyclic group Chemical group 0.000 claims 2
- 238000006731 degradation reaction Methods 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000009396 hybridization Methods 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 229960004469 methoxsalen Drugs 0.000 claims 2
- 150000003212 purines Chemical class 0.000 claims 2
- 229940083082 pyrimidine derivative acting on arteriolar smooth muscle Drugs 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 230000007515 enzymatic degradation Effects 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 229940083251 peripheral vasodilators purine derivative Drugs 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/551,305 US6573048B1 (en) | 2000-04-18 | 2000-04-18 | Degradable nucleic acid probes and nucleic acid detection methods |
| PCT/US2001/012701 WO2001079563A2 (en) | 2000-04-18 | 2001-04-18 | Degradable nucleic acid probes and nucleic acid detection methods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004501615A JP2004501615A (ja) | 2004-01-22 |
| JP2004501615A5 true JP2004501615A5 (https=) | 2005-02-03 |
Family
ID=24200719
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001576947A Pending JP2004501615A (ja) | 2000-04-18 | 2001-04-18 | 分解可能な核酸プローブおよび核酸検出方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6573048B1 (https=) |
| EP (1) | EP1280938A2 (https=) |
| JP (1) | JP2004501615A (https=) |
| KR (1) | KR20030005289A (https=) |
| CN (1) | CN1432069A (https=) |
| CA (1) | CA2406096A1 (https=) |
| CZ (1) | CZ20023749A3 (https=) |
| HU (1) | HUP0301031A2 (https=) |
| MX (1) | MXPA02010283A (https=) |
| PL (1) | PL362928A1 (https=) |
| WO (1) | WO2001079563A2 (https=) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002059137A1 (en) * | 2000-11-03 | 2002-08-01 | Isis Pharmaceuticals, Inc. | Nuclease-based method for detecting and quantitating oligonucleotides |
| EP1513958B1 (en) | 2002-06-14 | 2011-08-10 | Gen-Probe Incorporated | Compositions for detecting hepatitis b virus |
| US8575327B2 (en) * | 2003-06-12 | 2013-11-05 | Alnylam Pharmaceuticals, Inc. | Conserved HBV and HCV sequences useful for gene silencing |
| EP2290071B1 (en) * | 2004-05-28 | 2014-12-31 | Asuragen, Inc. | Methods and compositions involving microRNA |
| US20060078894A1 (en) * | 2004-10-12 | 2006-04-13 | Winkler Matthew M | Methods and compositions for analyzing nucleic acids |
| CA2857881A1 (en) | 2004-11-12 | 2006-12-28 | Asuragen, Inc. | Methods and compositions involving mirna and mirna inhibitor molecules |
| JP4712814B2 (ja) * | 2005-11-17 | 2011-06-29 | 独立行政法人科学技術振興機構 | 特定の塩基配列の標的核酸類を検出する方法、及び検出のための核酸類セット |
| US20080131878A1 (en) * | 2006-12-05 | 2008-06-05 | Asuragen, Inc. | Compositions and Methods for the Detection of Small RNA |
| EP2104734A2 (en) * | 2006-12-08 | 2009-09-30 | Asuragen, INC. | Mir-20 regulated genes and pathways as targets for therapeutic intervention |
| WO2008073915A2 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | Micrornas differentially expressed in leukemia and uses thereof |
| CA2671270A1 (en) * | 2006-12-29 | 2008-07-17 | Asuragen, Inc. | Mir-16 regulated genes and pathways as targets for therapeutic intervention |
| US20090232893A1 (en) * | 2007-05-22 | 2009-09-17 | Bader Andreas G | miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION |
| JP2010529966A (ja) * | 2007-06-08 | 2010-09-02 | アシュラジェン インコーポレイテッド | 治療的介入の標的としてｍｉＲ−３４によって調節される遺伝子および経路 |
| US8361714B2 (en) * | 2007-09-14 | 2013-01-29 | Asuragen, Inc. | Micrornas differentially expressed in cervical cancer and uses thereof |
| US20090186015A1 (en) * | 2007-10-18 | 2009-07-23 | Latham Gary J | Micrornas differentially expressed in lung diseases and uses thereof |
| US8071562B2 (en) | 2007-12-01 | 2011-12-06 | Mirna Therapeutics, Inc. | MiR-124 regulated genes and pathways as targets for therapeutic intervention |
| WO2009126726A1 (en) * | 2008-04-08 | 2009-10-15 | Asuragen, Inc | Methods and compositions for diagnosing and modulating human papillomavirus (hpv) |
| US8258111B2 (en) * | 2008-05-08 | 2012-09-04 | The Johns Hopkins University | Compositions and methods related to miRNA modulation of neovascularization or angiogenesis |
| WO2010056577A1 (en) * | 2008-11-17 | 2010-05-20 | Magic Technologies, Inc. | Methods and compositions in particle-based detection of target molecules using linking molecules |
| US9469868B2 (en) * | 2008-11-17 | 2016-10-18 | Headway Technologies, Inc. | Methods and compositions in particle-based detection of target molecules using covalent bond forming reactive pairs |
| WO2012112563A2 (en) | 2011-02-16 | 2012-08-23 | Magic Technologies, Inc. | Methods and compositions for the target-localized anchoring of detectable label |
| WO2013040251A2 (en) | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease |
| CN102703584B (zh) * | 2012-04-20 | 2013-08-21 | 中国人民解放军第四军医大学 | 自组装聚合分支dna探针的制备方法 |
| US9738925B2 (en) * | 2013-03-15 | 2017-08-22 | Lyle J. Arnold | Methods for immobilizing target nucleic acids utilizing combinatorial capture probes |
| JP6210484B2 (ja) * | 2013-06-25 | 2017-10-11 | 国立大学法人北陸先端科学技術大学院大学 | 鎖交換された二重鎖オリゴヌクレオチドの製造方法 |
| GB201401885D0 (en) * | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| TWI710633B (zh) | 2014-11-10 | 2020-11-21 | 美商阿尼拉製藥公司 | Ｂ型肝炎病毒（ＨＢＶ）ｉＲＮＡ組成物及其用途方法 |
| GB2570412A (en) * | 2016-08-31 | 2019-07-24 | Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
| US11324820B2 (en) | 2017-04-18 | 2022-05-10 | Alnylam Pharmaceuticals, Inc. | Methods for the treatment of subjects having a hepatitis b virus (HBV) infection |
| CA3106701A1 (en) | 2018-08-13 | 2020-02-20 | Alnylam Pharmaceuticals, Inc. | Hepatitis b virus (hbv) dsrna agent compositions and methods of use thereof |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| CN110295235A (zh) * | 2019-07-26 | 2019-10-01 | 合肥欧创基因生物科技有限公司 | 一种双标记探针及其在snp分型及肿瘤突变的应用 |
| WO2021155063A1 (en) | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4599303A (en) * | 1983-12-12 | 1986-07-08 | Hri Associates, Inc. | Nucleic acid hybridization assay employing probes crosslinkable to target sequences |
| US4826967A (en) | 1987-06-16 | 1989-05-02 | Naxcor | Psoralen-nucleoside adducts and method for their preparation |
| US5403711A (en) * | 1987-11-30 | 1995-04-04 | University Of Iowa Research Foundation | Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved |
| US6004826A (en) * | 1988-07-20 | 1999-12-21 | David Segev | Repair-mediated process for amplifying and detecting nucleic acid sequences |
| US5082934A (en) | 1989-04-05 | 1992-01-21 | Naxcor | Coumarin derivatives for use as nucleotide crosslinking reagents |
| US5767259A (en) * | 1994-12-27 | 1998-06-16 | Naxcor | Oligonucleotides containing base-free linking groups with photoactivatable side chains |
| WO1994024120A1 (en) | 1993-04-13 | 1994-10-27 | Naxcor | Non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents |
| US5616464A (en) | 1994-12-27 | 1997-04-01 | Naxcor | Nucleic acid sequence detection employing amplification probes |
| US5843650A (en) * | 1995-05-01 | 1998-12-01 | Segev; David | Nucleic acid detection and amplification by chemical linkage of oligonucleotides |
| AU724506B2 (en) | 1995-11-03 | 2000-09-21 | Naxcor | Double-stranded conformational polymorphism analysis |
| CN1285879A (zh) | 1998-01-09 | 2001-02-28 | 美国3M公司 | 可特异性酶切断裂的多核苷酸底物和测试方法 |
| WO1999042616A1 (en) | 1998-02-20 | 1999-08-26 | Dade Behring Inc. | Methods for determining concentrations of nucleic acids |
| US6124099A (en) * | 1998-06-22 | 2000-09-26 | The University Of Vermont And State Agricultural College | Method for placing a photo-cross-linking agent at specific internal sites within the sequence of synthetic strands of ribonucleic acids |
| US6303799B1 (en) | 1998-11-10 | 2001-10-16 | Naxcor | Polynucleotide crosslinking agents |
-
2000
- 2000-04-18 US US09/551,305 patent/US6573048B1/en not_active Expired - Fee Related
-
2001
- 2001-04-18 PL PL01362928A patent/PL362928A1/xx unknown
- 2001-04-18 KR KR1020027014025A patent/KR20030005289A/ko not_active Withdrawn
- 2001-04-18 WO PCT/US2001/012701 patent/WO2001079563A2/en not_active Ceased
- 2001-04-18 CA CA002406096A patent/CA2406096A1/en not_active Abandoned
- 2001-04-18 CN CN01810638A patent/CN1432069A/zh active Pending
- 2001-04-18 MX MXPA02010283A patent/MXPA02010283A/es unknown
- 2001-04-18 JP JP2001576947A patent/JP2004501615A/ja active Pending
- 2001-04-18 EP EP01932581A patent/EP1280938A2/en not_active Withdrawn
- 2001-04-18 HU HU0301031A patent/HUP0301031A2/hu unknown
- 2001-04-18 CZ CZ20023749A patent/CZ20023749A3/cs unknown
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