JP2004029205A - レーザ走査型顕微鏡 - Google Patents
レーザ走査型顕微鏡 Download PDFInfo
- Publication number
- JP2004029205A JP2004029205A JP2002182798A JP2002182798A JP2004029205A JP 2004029205 A JP2004029205 A JP 2004029205A JP 2002182798 A JP2002182798 A JP 2002182798A JP 2002182798 A JP2002182798 A JP 2002182798A JP 2004029205 A JP2004029205 A JP 2004029205A
- Authority
- JP
- Japan
- Prior art keywords
- light source
- laser
- light
- laser scanning
- scanning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003287 optical effect Effects 0.000 claims description 68
- 239000004065 semiconductor Substances 0.000 claims description 63
- 239000013307 optical fiber Substances 0.000 claims description 47
- 238000001514 detection method Methods 0.000 claims description 35
- 230000010287 polarization Effects 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 4
- 238000007493 shaping process Methods 0.000 description 31
- 239000000835 fiber Substances 0.000 description 27
- 238000010586 diagram Methods 0.000 description 8
- 238000003384 imaging method Methods 0.000 description 5
- 230000004075 alteration Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 201000009310 astigmatism Diseases 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002182798A JP2004029205A (ja) | 2002-06-24 | 2002-06-24 | レーザ走査型顕微鏡 |
| US10/602,361 US7015485B2 (en) | 2002-06-24 | 2003-06-23 | Laser scanning microscope, semiconductor laser light source unit, scanning unit for a laser scanning microscope, and method of connecting semiconductor light source to scanning microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002182798A JP2004029205A (ja) | 2002-06-24 | 2002-06-24 | レーザ走査型顕微鏡 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004029205A true JP2004029205A (ja) | 2004-01-29 |
| JP2004029205A5 JP2004029205A5 (enExample) | 2005-10-20 |
Family
ID=31179201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002182798A Pending JP2004029205A (ja) | 2002-06-24 | 2002-06-24 | レーザ走査型顕微鏡 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US7015485B2 (enExample) |
| JP (1) | JP2004029205A (enExample) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005308974A (ja) * | 2004-04-20 | 2005-11-04 | Olympus Corp | 走査型レーザ顕微鏡装置 |
| JP2006221167A (ja) * | 2005-02-09 | 2006-08-24 | Leica Microsystems (Schweiz) Ag | 手術顕微鏡用蛍光/赤外線装置 |
| JP2009192800A (ja) * | 2008-02-14 | 2009-08-27 | Yokogawa Electric Corp | 共焦点スキャナ顕微鏡 |
| CN103698307A (zh) * | 2013-12-06 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | 激光扫描共聚焦显微系统 |
| WO2015121911A1 (en) | 2014-02-17 | 2015-08-20 | Sony Corporation | Scanning unit, laser scanning microscope, and temperature adjustment method |
| CN107886823A (zh) * | 2017-11-15 | 2018-04-06 | 中国工程物理研究院电子工程研究所 | 一种优化集成式单光路激光电离效应模拟系统 |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10332063A1 (de) * | 2003-07-11 | 2005-01-27 | Carl Zeiss Jena Gmbh | Laser-Scanning-Mikroskop |
| JP4481082B2 (ja) * | 2004-05-24 | 2010-06-16 | オリンパス株式会社 | 顕微鏡観察方法および顕微鏡観察装置 |
| DE102004034961A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit linienförmiger Abtastung und Verwendung |
| DE102004034959A1 (de) * | 2004-07-16 | 2006-02-16 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit punktförmiger Lichtquellenverteilung und Verwendung |
| DE102004034987A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop und Verwendung |
| KR100741110B1 (ko) * | 2006-02-15 | 2007-07-19 | 삼성에스디아이 주식회사 | 광 파이버 및 플라즈마 디스플레이 패널의 전극 형성 방법 |
| DE102006034909A1 (de) * | 2006-07-28 | 2008-01-31 | Carl Zeiss Microimaging Gmbh | Verfahren zur Laser-Scanning-Mikroskopie und Strahlvereiniger |
| US7817275B2 (en) * | 2007-03-07 | 2010-10-19 | University Of Delaware | Scanning optical microscope with long working distance objective |
| CN101828139A (zh) * | 2007-09-05 | 2010-09-08 | 致茂电子股份有限公司 | 具有波长转换磷光体的光源 |
| US8111455B2 (en) * | 2007-11-26 | 2012-02-07 | Olympus Corporation | Light source apparatus and laser scanning microscope |
| US9778188B2 (en) * | 2009-03-11 | 2017-10-03 | Industrial Technology Research Institute | Apparatus and method for detection and discrimination molecular object |
| US9482615B2 (en) * | 2010-03-15 | 2016-11-01 | Industrial Technology Research Institute | Single-molecule detection system and methods |
| US8865078B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| US8865077B2 (en) | 2010-06-11 | 2014-10-21 | Industrial Technology Research Institute | Apparatus for single-molecule detection |
| WO2012006558A2 (en) * | 2010-07-08 | 2012-01-12 | Fei Company | Charged particle beam processing system with visual and infrared imaging |
| JP5926966B2 (ja) * | 2012-01-30 | 2016-05-25 | オリンパス株式会社 | 蛍光観察装置 |
| JPWO2016098139A1 (ja) * | 2014-12-16 | 2017-11-02 | オリンパス株式会社 | レーザ走査型観察装置 |
| CN106568755A (zh) * | 2016-11-06 | 2017-04-19 | 浙江大学 | 一种近红外激光扫描共聚焦显微成像系统 |
| WO2018089839A1 (en) | 2016-11-10 | 2018-05-17 | The Trustees Of Columbia University In The City Of New York | Rapid high-resolution imaging methods for large samples |
| US10378006B2 (en) | 2017-04-19 | 2019-08-13 | The Florida International University Board Of Trustees | Near-infrared ray exposure system for biological studies |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6903347B2 (en) * | 1994-07-15 | 2005-06-07 | Stephen C. Baer | Superresolution in microlithography and fluorescence microscopy |
| US6167173A (en) * | 1997-01-27 | 2000-12-26 | Carl Zeiss Jena Gmbh | Laser scanning microscope |
| JPH1195120A (ja) * | 1997-09-19 | 1999-04-09 | Olympus Optical Co Ltd | 顕微鏡の観察方法 |
| JPH11231222A (ja) | 1998-01-27 | 1999-08-27 | Carl Zeiss Jena Gmbh | 走査ユニット付顕微鏡、そのための配置および操作方法 |
| CA2280398C (en) * | 1998-10-26 | 2009-01-20 | Lothar Lilge | A semiconductor based excitation illuminator for fluorescence and phosphorescence microscopy |
| JP4680337B2 (ja) * | 1999-09-20 | 2011-05-11 | オリンパス株式会社 | 走査型レーザ顕微鏡 |
| DE10004233B4 (de) * | 2000-02-01 | 2005-02-17 | Leica Microsystems Heidelberg Gmbh | Mikroskop-Aufbau |
| US6844963B2 (en) * | 2000-03-23 | 2005-01-18 | Olympus Optical Co., Ltd. | Double-resonance-absorption microscope |
| US6423956B1 (en) * | 2000-07-28 | 2002-07-23 | Optical Biopsy Technologies | Fiber-coupled, high-speed, integrated, angled-dual-axis confocal scanning microscopes employing vertical cross-section scanning |
| DE10044636A1 (de) * | 2000-09-08 | 2002-03-21 | Leica Microsystems | Optische Anordnung zur Beleuchtung von Objekten für konfokale Rastermikroskope |
| JP4812179B2 (ja) * | 2001-03-13 | 2011-11-09 | オリンパス株式会社 | レーザ顕微鏡 |
| JP3887188B2 (ja) * | 2001-08-08 | 2007-02-28 | 株式会社ミツトヨ | プローブのアライメント調整装置、その装置を備えた測定機およびプローブのアライメント調整方法 |
| JP4939703B2 (ja) * | 2001-08-21 | 2012-05-30 | オリンパス株式会社 | 走査型レーザー顕微鏡 |
| US6963398B2 (en) * | 2001-10-03 | 2005-11-08 | Olympus Optical Co., Ltd. | Laser scanning microscope |
| US7196843B2 (en) * | 2002-03-27 | 2007-03-27 | Olympus Optical Co., Ltd. | Confocal microscope apparatus |
-
2002
- 2002-06-24 JP JP2002182798A patent/JP2004029205A/ja active Pending
-
2003
- 2003-06-23 US US10/602,361 patent/US7015485B2/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005308974A (ja) * | 2004-04-20 | 2005-11-04 | Olympus Corp | 走査型レーザ顕微鏡装置 |
| JP2006221167A (ja) * | 2005-02-09 | 2006-08-24 | Leica Microsystems (Schweiz) Ag | 手術顕微鏡用蛍光/赤外線装置 |
| JP2009192800A (ja) * | 2008-02-14 | 2009-08-27 | Yokogawa Electric Corp | 共焦点スキャナ顕微鏡 |
| CN103698307A (zh) * | 2013-12-06 | 2014-04-02 | 中国科学院苏州生物医学工程技术研究所 | 激光扫描共聚焦显微系统 |
| WO2015121911A1 (en) | 2014-02-17 | 2015-08-20 | Sony Corporation | Scanning unit, laser scanning microscope, and temperature adjustment method |
| CN107886823A (zh) * | 2017-11-15 | 2018-04-06 | 中国工程物理研究院电子工程研究所 | 一种优化集成式单光路激光电离效应模拟系统 |
| CN107886823B (zh) * | 2017-11-15 | 2024-05-10 | 中国工程物理研究院电子工程研究所 | 一种优化集成式单光路激光电离效应模拟系统 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20040061073A1 (en) | 2004-04-01 |
| US7015485B2 (en) | 2006-03-21 |
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Legal Events
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