JP2002505071A5 - - Google Patents

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Publication number
JP2002505071A5
JP2002505071A5 JP2000519105A JP2000519105A JP2002505071A5 JP 2002505071 A5 JP2002505071 A5 JP 2002505071A5 JP 2000519105 A JP2000519105 A JP 2000519105A JP 2000519105 A JP2000519105 A JP 2000519105A JP 2002505071 A5 JP2002505071 A5 JP 2002505071A5
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JP
Japan
Prior art keywords
sequence
nucleic acid
binding
detecting
bind
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2000519105A
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English (en)
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JP2002505071A (ja
Filing date
Publication date
Priority claimed from DE19748690A external-priority patent/DE19748690A1/de
Priority claimed from DE19814001A external-priority patent/DE19814001A1/de
Priority claimed from DE19814828A external-priority patent/DE19814828A1/de
Application filed filed Critical
Priority claimed from PCT/EP1998/006961 external-priority patent/WO1999023250A2/de
Publication of JP2002505071A publication Critical patent/JP2002505071A/ja
Publication of JP2002505071A5 publication Critical patent/JP2002505071A5/ja
Pending legal-status Critical Current

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Description

【特許請求の範囲】
【請求項１】 核酸の検出方法であって、
レポーター基および消光基を含んでおり、配列Ａと配列Ｃとの間に位置する第３配列（Ｂ）またはその相補体（Ｂ’）に結合することができる結合配列Ｄを有するプローブの存在下に５’ヌクレアーゼ活性を有するポリメラーゼを使用して、一方が、前記核酸の一方の鎖の第１結合配列（Ａ）に結合することができる、かつもう一方が、Ａの３’方向に位置し、Ａと重複しない配列Ｃと本質的に相補的である第２結合配列（Ｃ’）に結合することができる、２つのプライマーの助けにより前記核酸の一部の多数の増幅物を作製する工程、並びに
レポーター基の解離により生ずるシグナルを測定することにより核酸を検出する工程、
を含み、
前記プライマーの助けにより形成される増幅物が、７５ヌクレオチド未満の長さを有するものである、核酸の検出方法。
【請求項２】 プローブの結合配列Ｄがプライマーの結合配列の１つと重複しないものである請求項１記載の方法。＿
JP2000519105A 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法 Pending JP2002505071A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE19748690A DE19748690A1 (de) 1997-11-04 1997-11-04 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814001A DE19814001A1 (de) 1998-03-28 1998-03-28 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19748690.8 1998-04-02
DE19814001.0 1998-04-02
DE19814828A DE19814828A1 (de) 1998-04-02 1998-04-02 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828.3 1998-04-02
PCT/EP1998/006961 WO1999023250A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren

Publications (2)

Publication Number Publication Date
JP2002505071A JP2002505071A (ja) 2002-02-19
JP2002505071A5 true JP2002505071A5 (ja) 2006-01-05

Family

ID=27217887

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2000519104A Pending JP2002509694A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519599A Pending JP2001521765A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519105A Pending JP2002505071A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法

Family Applications Before (2)

Application Number Title Priority Date Filing Date
JP2000519104A Pending JP2002509694A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法
JP2000519599A Pending JP2001521765A (ja) 1997-11-04 1998-11-03 特異的かつ高感度な核酸検出方法

Country Status (6)

Country Link
US (1) US7105318B2 (ja)
EP (3) EP1029077A2 (ja)
JP (3) JP2002509694A (ja)
AU (3) AU741141B2 (ja)
CA (3) CA2308368C (ja)
WO (3) WO1999023250A2 (ja)

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US9376727B2 (en) 2010-05-25 2016-06-28 Qiagen Gaithersburg, Inc. Fast results hybrid capture assay and associated strategically truncated probes
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