JP2001501476A - 脊椎動物Smoothenedタンパク質 - Google Patents
脊椎動物Smoothenedタンパク質Info
- Publication number
- JP2001501476A JP2001501476A JP10516721A JP51672198A JP2001501476A JP 2001501476 A JP2001501476 A JP 2001501476A JP 10516721 A JP10516721 A JP 10516721A JP 51672198 A JP51672198 A JP 51672198A JP 2001501476 A JP2001501476 A JP 2001501476A
- Authority
- JP
- Japan
- Prior art keywords
- vertebrate
- smoothened
- antibody
- sequence
- vsmo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 208000024891 symptom Diseases 0.000 description 1
- 210000001779 taste bud Anatomy 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 229960002175 thyroglobulin Drugs 0.000 description 1
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- 235000013619 trace mineral Nutrition 0.000 description 1
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- 239000012581 transferrin Substances 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 210000001364 upper extremity Anatomy 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 208000024719 uterine cervix neoplasm Diseases 0.000 description 1
- 230000002861 ventricular Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Saccharide Compounds (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.単離した脊椎動物Smoothened 2.配列番号4の1から787残基を含む天然配列脊椎動物Smoothenedと少なくとも8 0%の配列同一性を有する単離した脊椎動物Smoothened。 3.上記Smoothenedが少なくとも90%の同一性を有する請求項2の脊椎動物Smoot hened。 4.上記Smoothenedが少なくとも95%の同一性を有する請求項3の脊椎動物Smoot hened。 5.配列番号4のアミノ酸配列を含む単離した天然配列脊椎動物Smoothened。 6.配列番号2のアミノ酸配列を含む単離した天然配列脊椎動物Smoothened。 7.異種アミノ酸配列と融合した請求項1の脊椎動物Smoothenedを含むキメラ分 子。 8.上記異種アミノ酸配列がエピトープタグ配列である請求項7のキメラ分子。 9.請求項1の脊椎動物Smoothenedに特異的に結合する抗体。 10.上記抗体がモノクローナル抗体である請求項9の抗体。 11.中和抗体である請求項9の抗体。 12.アゴニスト抗体である請求項9の抗体。 13.脊椎動物Smoothenedをコードする単離した核酸。 14.上記核酸が配列番号4のアミノ酸配列を含む天然配列脊椎動物Smoothenedを コードする請求項13の核酸。 15.上記核酸か配列番号2のアミノ酸配列を含む天然配列脊椎動物Smoothenedを コードする請求項13の核酸。 16.請求項13の核酸を含むベクター。 17.該ベクターを用いてトランスフォームされたホスト細胞によって認識される 制御配列に機能的に連結されている請求項16のベクター。 18.請求項16のベクターを含む宿主細胞。 19.請求項18の宿主細胞を培養することを含む脊椎動物Smoothenedの生産に効果 的な脊椎動物Smoothenedをコードする核酸を用いる方法。 20.さらに宿主カルチャーから脊椎動物Smoothenedを回収することを含む請求項 19の方法。 21.容器と上記容器内に含まれる組成物を含む製造品であり、該組成物は脊椎動 物Smoothenedまたは脊椎動物Smoothened抗体を含むことを特徴とする製造品。 22.さらにin vivoまたはex vivoでの脊椎動物Smoothenedまたは脊椎動物Smooth ened抗体を用いるための説明書を含む請求項21の製造品。 23.脊椎動物Smoothenedをコードする核酸を発現する細胞を含む非ヒトトランス ジェニック動物。 24.マウスまたはラットである請求項23の動物。 25.脊椎動物Smoothenedをコードする改変された遺伝子を有する細胞を含む非ヒ トノックアウト動物。 26.マウスまたはラットである請求項25の動物。 27.脊椎動物Smoothenedタンパク質及び脊椎動物Patchedタンパク質を含むタン パク質複合体。 28.さらに脊椎動物Hedgehogタンパク質を含む請求項27のタンパク質複合体。 29.脊椎動物Hedgehogタンパク質が脊椎動物Patchedタンパク質に結合するが脊 椎動物Smoothenedタンパク質には結合しない請求項28のタンパク質複合体。 30.脊椎動物Hedgehogタンパク質に対するレセプターである請求項27のタンパ ク質複合体。 31.脊椎動物Smoothenedに結合する脊椎動物Patched。 32.天然配列脊椎動物Patchedと100%より小さい配列同一性を有する請求項31 の脊椎動物Patched。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/720,484 | 1996-09-30 | ||
US08/720,484 US5990281A (en) | 1996-09-30 | 1996-09-30 | Vertebrate smoothened proteins |
PCT/US1997/017433 WO1998014475A1 (en) | 1996-09-30 | 1997-09-29 | Vertebrate smoothened proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001501476A true JP2001501476A (ja) | 2001-02-06 |
JP4671371B2 JP4671371B2 (ja) | 2011-04-13 |
Family
ID=24894172
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP51672198A Expired - Lifetime JP4671371B2 (ja) | 1996-09-30 | 1997-09-29 | 脊椎動物Smoothenedタンパク質 |
Country Status (12)
Country | Link |
---|---|
US (2) | US5990281A (ja) |
EP (1) | EP0939769B1 (ja) |
JP (1) | JP4671371B2 (ja) |
AT (1) | ATE309270T1 (ja) |
AU (1) | AU725701B2 (ja) |
CA (1) | CA2267181A1 (ja) |
DE (1) | DE69734596T2 (ja) |
DK (1) | DK0939769T3 (ja) |
ES (1) | ES2252795T3 (ja) |
IL (1) | IL129139A (ja) |
WO (1) | WO1998014475A1 (ja) |
ZA (1) | ZA978503B (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112638933A (zh) * | 2018-07-11 | 2021-04-09 | 海德格霍格有限公司 | Smo蛋白的特异性表位、识别该表位的抗体以及包含该抗体的组合物 |
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AU8173098A (en) | 1997-06-27 | 1999-01-19 | Ontogeny, Inc. | Neuroprotective methods and reagents |
US7144997B2 (en) | 1997-07-24 | 2006-12-05 | Curis, Inc. | Vertebrate embryonic patterning-inducing proteins, compositions and uses related therto |
US7361336B1 (en) | 1997-09-18 | 2008-04-22 | Ivan Bergstein | Methods of cancer therapy targeted against a cancer stem line |
US6639051B2 (en) | 1997-10-20 | 2003-10-28 | Curis, Inc. | Regulation of epithelial tissue by hedgehog-like polypeptides, and formulations and uses related thereto |
WO1999029854A1 (en) | 1997-12-08 | 1999-06-17 | Ontogeny, Inc. | Human patched genes and proteins, and uses related thereto |
US6884770B1 (en) | 1998-11-06 | 2005-04-26 | Curis, Inc. | Methods and compositions for treating or preventing peripheral neuropathies |
EP2783700A1 (en) * | 2006-09-07 | 2014-10-01 | Stemline Therapeutics, Inc. | Cancer stem cell-targeted cancer therapy |
AU2010289400B2 (en) | 2009-09-02 | 2014-10-23 | Curis, Inc. | Mutant smoothened and methods of using the same |
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FR2413974A1 (fr) * | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
US4342566A (en) * | 1980-02-22 | 1982-08-03 | Scripps Clinic & Research Foundation | Solid phase anti-C3 assay for detection of immune complexes |
US4399216A (en) * | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
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ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
US4419446A (en) * | 1980-12-31 | 1983-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant DNA process utilizing a papilloma virus DNA as a vector |
NZ201705A (en) * | 1981-08-31 | 1986-03-14 | Genentech Inc | Recombinant dna method for production of hepatitis b surface antigen in yeast |
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US4601978A (en) * | 1982-11-24 | 1986-07-22 | The Regents Of The University Of California | Mammalian metallothionein promoter system |
AU2353384A (en) * | 1983-01-19 | 1984-07-26 | Genentech Inc. | Amplification in eukaryotic host cells |
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US4676980A (en) * | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
EP0272253A4 (en) * | 1986-03-07 | 1990-02-05 | Massachusetts Inst Technology | METHOD FOR IMPROVING GLYCOPROTE INSTABILITY. |
GB8724885D0 (en) * | 1987-10-23 | 1987-11-25 | Binns M M | Fowlpox virus promotors |
KR0154872B1 (ko) * | 1987-12-21 | 1998-10-15 | 로버트 에이. 아미테이지 | 발아하는 식물종자의 아크로박테리움 매개된 형질전환 |
CA2062795A1 (en) * | 1989-06-29 | 1990-12-30 | Michael W. Fanger | Bispecific reagents for aids therapy |
DK168302B1 (da) * | 1989-06-29 | 1994-03-07 | Danisco | Fremgangsmåde til indføring af molekyler, især genetisk materiale i planteceller |
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-
1996
- 1996-09-30 US US08/720,484 patent/US5990281A/en not_active Expired - Lifetime
-
1997
- 1997-09-22 ZA ZA978503A patent/ZA978503B/xx unknown
- 1997-09-29 EP EP97944533A patent/EP0939769B1/en not_active Revoked
- 1997-09-29 DK DK97944533T patent/DK0939769T3/da active
- 1997-09-29 AT AT97944533T patent/ATE309270T1/de active
- 1997-09-29 DE DE69734596T patent/DE69734596T2/de not_active Revoked
- 1997-09-29 CA CA002267181A patent/CA2267181A1/en not_active Abandoned
- 1997-09-29 IL IL129139A patent/IL129139A/en not_active IP Right Cessation
- 1997-09-29 ES ES97944533T patent/ES2252795T3/es not_active Expired - Lifetime
- 1997-09-29 WO PCT/US1997/017433 patent/WO1998014475A1/en active IP Right Grant
- 1997-09-29 JP JP51672198A patent/JP4671371B2/ja not_active Expired - Lifetime
- 1997-09-29 AU AU46005/97A patent/AU725701B2/en not_active Expired
-
1999
- 1999-09-20 US US09/398,239 patent/US6407216B1/en not_active Expired - Lifetime
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112638933A (zh) * | 2018-07-11 | 2021-04-09 | 海德格霍格有限公司 | Smo蛋白的特异性表位、识别该表位的抗体以及包含该抗体的组合物 |
JP2021530250A (ja) * | 2018-07-11 | 2021-11-11 | ヘッジホッグ,インコーポレイテッド | Smoタンパク質の特異的エピトープ、これを認知する抗体及びこれを含む組成物 |
JP7438560B2 (ja) | 2018-07-11 | 2024-02-27 | ヘッジホッグ,インコーポレイテッド | Smoタンパク質の特異的エピトープ、これを認知する抗体及びこれを含む組成物 |
Also Published As
Publication number | Publication date |
---|---|
CA2267181A1 (en) | 1998-04-09 |
DE69734596D1 (de) | 2005-12-15 |
AU725701B2 (en) | 2000-10-19 |
ATE309270T1 (de) | 2005-11-15 |
ZA978503B (en) | 1999-03-23 |
EP0939769B1 (en) | 2005-11-09 |
DE69734596T2 (de) | 2006-07-20 |
US6407216B1 (en) | 2002-06-18 |
AU4600597A (en) | 1998-04-24 |
WO1998014475A1 (en) | 1998-04-09 |
IL129139A (en) | 2007-12-03 |
ES2252795T3 (es) | 2006-05-16 |
IL129139A0 (en) | 2000-02-17 |
EP0939769A1 (en) | 1999-09-08 |
DK0939769T3 (da) | 2006-03-13 |
US5990281A (en) | 1999-11-23 |
JP4671371B2 (ja) | 2011-04-13 |
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