JP2000512150A - 細胞を形質転換するための方法および組成物 - Google Patents
細胞を形質転換するための方法および組成物Info
- Publication number
- JP2000512150A JP2000512150A JP10501738A JP50173898A JP2000512150A JP 2000512150 A JP2000512150 A JP 2000512150A JP 10501738 A JP10501738 A JP 10501738A JP 50173898 A JP50173898 A JP 50173898A JP 2000512150 A JP2000512150 A JP 2000512150A
- Authority
- JP
- Japan
- Prior art keywords
- vector
- sequences
- lox
- donor
- receptor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13041—Use of virus, viral particle or viral elements as a vector
- C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.部位特異的組換えを起こす方法であって、組換え酵素を(a)2つの不和合 性lox配列、L1およびL2を含んで成る受容体ベクター、および(b)L1お よびL2配列、またはL1およびL2配列と和合性である配列によりフランキン グ化されたある選択したDNAを含んで成る供与体ベクターと接触させ、これに より選択したDNAを和合性lox配列で組換えることにより供与体ベクターから 受容体ベクターへ転移させることを含んで成る上記方法。 2.受容体ベクターがレトロウイルスベクターおよびアデノ随伴ベクターから成 る群から選択される、請求の範囲第1項に記載の方法。 3.L1およびL2がそれらのスペーサー配列中の1個以上の点突然変異により 異なり、それらを不和合性とする請求の範囲第1項に記載の方法。 4.L1およびL2が、LoxP1およびLox511ヌクレオチド配列(配列番号1および 2)を含んで成り、そして組換え酵素がCreである請求の範囲第1項に記載の方法 。 5.受容体ベクターがレトロウイルスベクターを含んで成る請求の範囲第1項に 記載の方法。 6.選択したDNAで細胞を形質転換する方法であって、任意の順序で、(a) 細胞に、細胞のゲノムに組込まれる受容体ベクターを導入し、この受容体ベクタ ーは2つの不和合性lox配列L1およびL2を含んで成り、(b)細胞に、L1 およびL2配列、またはL1およびL2配列と和合性の配列により挟まれた選択 したDNAを含んで成る供与体ベクターを導入し、そして (c)L1およびL2をCreと接触させ、これにより選択したDNAの供与体ベ クターから受容体ベクターへの転移を引き起こす、 工程を含んで成る、上記方法。 7.受容体ベクターが、アデノ随伴ウイルスベクターを含んで成る請求の範囲第 6項に記載の方法。 8.L1およびL2が、それらのスペーサー配列中で少なくとも1個のヌクレオ チド付加、欠失または置換により異なり、それらを不和合性とする請求の範囲第 6項に記載の方法。 9.L1またはL2のいずれかが、LoxP1ヌクレオチド配列(配列番号1)を含 んで成る、請求の範囲第6項に記載の方法。 10.L1またはL2のいずれかが、Lox511ヌクレオチド配列(配列番号2)を 含んで成る、請求の範囲第6項に記載の方法。 11.L1およびL2が、LoxP1およびLox511ヌクレオチド配列(配列番号1およ び2)を含んで成る請求の範囲第6項に記載の方法。 12.L1およびL2が同方向を有する、請求の範囲第6項に記載の方法。 13.さらに細胞にCre組換え酵素をコードする遺伝子を導入する工程を含んで 成る、請求の範囲第6項に記載の方法。 14.Cre組換え酵素をコードする遺伝子が発現ベクターに含まれている、請求 の範囲第13項に記載の方法。 15.Cre組換え酵素をコードする遺伝子が、受容体ベクターまたは供与体ベク ターのいずれかに含まれている、請求の範囲第13項に記載の方法。 16.供与体ベクターが細胞に電気穿孔法により導入される、請求の範 囲第6項に記載の方法。 17.供与体ベクターおよびCre組換え酵素をコードする遺伝子の両方が、細胞 に電気穿孔法により導入される、請求の範囲第13項に記載の方法。 18.選択したDNAが治療用タンパク質をコードする導入遺伝子である請求の 範囲第6項に記載の方法。 19.タンパク質がβ−グロビンである、請求の範囲第18項に記載の方法。 20.供与体ベクター、受容体ベクター、または供与体および受容体ベクターの 両方が、さらに選択可能なマーカー遺伝子を含んで成る請求の範囲第6項に記載 の方法。 21.細胞が哺乳動物細胞である請求の範囲第6項に記載の方法。 22.2つの不和合性lox配列、L1およびL2を含んで成るレトロウイルスベ クターおよびアデノ随伴ベクターからなる群から選択されるベクター。 23.L1およびL2が、Lox P1およびLox 511ヌクレオチド配列(配列番号1 および2)を含んで成る、請求の範囲第22項に記載のベクター。 24.ベクターが選択可能なマーカー遺伝子をさらに含んで成る、請求の範囲第 22項に記載のベクター。 25.(a)宿主細胞のゲノムに組込むことができる受容体ベクター、この受容 体ベクターは2つの不和合性lox配列、L1およびL2を含んで成り: (b)L1およびL2配列、またはL1およびL2配列と和合性である 配列により挟まれた導入遺伝子を含んで成る供与体ベクター:および (c)和合性lox配列間の組換えを触媒できる組換え酵素、 を含んで成るCre/loxが媒介する遺伝子導入系。 26.受容体ベクターが、レトロウイルスベクターおよびアデノ随伴ベクターか ら成る群から選択される、請求の範囲第25項に記載の系。 27.L1およびL2がそれらのスペーサー配列中の1個以上の点突然変異によ り異なり、それらを不和合性とする請求の範囲第25項に記載の系。 28.L1およびL2が、LoxP1およびLox511ヌクレオチド配列(配列番号1およ び2)を含んで成る、請求の範囲第25項に記載の系。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US66408496A | 1996-06-14 | 1996-06-14 | |
US08/664,084 | 1996-06-14 | ||
US08/743,796 US5928914A (en) | 1996-06-14 | 1996-11-05 | Methods and compositions for transforming cells |
US08/743,796 | 1996-11-05 | ||
PCT/US1997/009954 WO1997047758A1 (en) | 1996-06-14 | 1997-06-06 | Methods and compositions for transforming cells |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009262993A Division JP5765547B2 (ja) | 1996-06-14 | 2009-11-18 | 細胞を形質転換するための方法および組成物 |
Publications (1)
Publication Number | Publication Date |
---|---|
JP2000512150A true JP2000512150A (ja) | 2000-09-19 |
Family
ID=24664451
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP10501738A Ceased JP2000512150A (ja) | 1996-06-14 | 1997-06-06 | 細胞を形質転換するための方法および組成物 |
JP2009262993A Expired - Lifetime JP5765547B2 (ja) | 1996-06-14 | 2009-11-18 | 細胞を形質転換するための方法および組成物 |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2009262993A Expired - Lifetime JP5765547B2 (ja) | 1996-06-14 | 2009-11-18 | 細胞を形質転換するための方法および組成物 |
Country Status (9)
Country | Link |
---|---|
US (1) | US5928914A (ja) |
EP (1) | EP0914457B1 (ja) |
JP (2) | JP2000512150A (ja) |
AT (1) | ATE404684T1 (ja) |
AU (1) | AU733016B2 (ja) |
CA (1) | CA2258007A1 (ja) |
DE (1) | DE69738905D1 (ja) |
HK (1) | HK1020752A1 (ja) |
WO (1) | WO1997047758A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010063463A (ja) * | 1996-06-14 | 2010-03-25 | Massachusetts Inst Of Technol <Mit> | 細胞を形質転換するための方法および組成物 |
JP2016136944A (ja) * | 2009-04-09 | 2016-08-04 | サンガモ バイオサイエンシーズ, インコーポレイテッド | 幹細胞への標的組込み |
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US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
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US6534314B1 (en) * | 1996-06-14 | 2003-03-18 | Massachusetts Institute Of Technology | Methods and compositions for transforming cells |
US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
WO1998048005A1 (en) | 1997-04-24 | 1998-10-29 | University Of Washington | Targeted gene modification by parvoviral vectors |
IL135776A0 (en) * | 1997-10-24 | 2001-05-20 | Life Technologies Inc | Recombinational cloning using nucleic acids having recombination sites |
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AU745960C (en) | 1997-11-18 | 2003-09-18 | Pioneer Hi-Bred International, Inc. | A novel method for the integration of foreign DNA into eukaryoticgenomes |
US7102055B1 (en) | 1997-11-18 | 2006-09-05 | Pioneer Hi-Bred International, Inc. | Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant |
ATE401410T1 (de) | 1997-11-18 | 2008-08-15 | Pioneer Hi Bred Int | Zusammensetzungen und verfahren für die genetische modifizierung von pflanzen |
CA2306053C (en) | 1997-11-18 | 2003-01-21 | Pioneer Hi-Bred International, Inc. | Mobilization of viral genomes from t-dna using site-specific recombination systems |
EP0939120A1 (en) * | 1998-02-27 | 1999-09-01 | Gesellschaft für biotechnologische Forschung mbH (GBF) | Method for marker-free repetitive DNA expression cassette exchange in the genome of cells or parts of cells |
AU1331800A (en) * | 1998-10-28 | 2000-05-15 | University Of Washington | Targeted gene modification by parvoviral vectors |
DE60042969D1 (de) | 1999-03-02 | 2009-10-29 | Life Technologies Corp | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren |
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WO2001005987A1 (fr) * | 1999-07-14 | 2001-01-25 | Transgenic Inc. | Vecteur piege et procede de piegeage de genes utilisant ledit vecteur |
DE19941186A1 (de) * | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
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US5928914A (en) * | 1996-06-14 | 1999-07-27 | Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University | Methods and compositions for transforming cells |
-
1996
- 1996-11-05 US US08/743,796 patent/US5928914A/en not_active Expired - Lifetime
-
1997
- 1997-06-06 JP JP10501738A patent/JP2000512150A/ja not_active Ceased
- 1997-06-06 WO PCT/US1997/009954 patent/WO1997047758A1/en active Application Filing
- 1997-06-06 AU AU33062/97A patent/AU733016B2/en not_active Expired
- 1997-06-06 CA CA002258007A patent/CA2258007A1/en not_active Abandoned
- 1997-06-06 EP EP97928910A patent/EP0914457B1/en not_active Expired - Lifetime
- 1997-06-06 DE DE69738905T patent/DE69738905D1/de not_active Expired - Lifetime
- 1997-06-06 AT AT97928910T patent/ATE404684T1/de not_active IP Right Cessation
-
1999
- 1999-11-12 HK HK99105258.8A patent/HK1020752A1/xx active IP Right Revival
-
2009
- 2009-11-18 JP JP2009262993A patent/JP5765547B2/ja not_active Expired - Lifetime
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2010063463A (ja) * | 1996-06-14 | 2010-03-25 | Massachusetts Inst Of Technol <Mit> | 細胞を形質転換するための方法および組成物 |
JP2016136944A (ja) * | 2009-04-09 | 2016-08-04 | サンガモ バイオサイエンシーズ, インコーポレイテッド | 幹細胞への標的組込み |
Also Published As
Publication number | Publication date |
---|---|
AU3306297A (en) | 1998-01-07 |
JP2010063463A (ja) | 2010-03-25 |
CA2258007A1 (en) | 1997-12-18 |
EP0914457A1 (en) | 1999-05-12 |
US5928914A (en) | 1999-07-27 |
WO1997047758A1 (en) | 1997-12-18 |
DE69738905D1 (de) | 2008-09-25 |
EP0914457B1 (en) | 2008-08-13 |
ATE404684T1 (de) | 2008-08-15 |
JP5765547B2 (ja) | 2015-08-19 |
HK1020752A1 (en) | 2000-05-19 |
AU733016B2 (en) | 2001-05-03 |
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