IL68733A - Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector - Google Patents

Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector

Info

Publication number
IL68733A
IL68733A IL68733A IL6873383A IL68733A IL 68733 A IL68733 A IL 68733A IL 68733 A IL68733 A IL 68733A IL 6873383 A IL6873383 A IL 6873383A IL 68733 A IL68733 A IL 68733A
Authority
IL
Israel
Prior art keywords
dna sequence
kluyveromyces cells
polypeptide
transformation
vector
Prior art date
Application number
IL68733A
Other languages
English (en)
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=19839766&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL68733(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of IL68733A publication Critical patent/IL68733A/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/43Sweetening agents, e.g. thaumatin, monellin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pyrane Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
IL68733A 1982-05-19 1983-05-19 Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector IL68733A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL8202091 1982-05-19

Publications (1)

Publication Number Publication Date
IL68733A true IL68733A (en) 1989-01-31

Family

ID=19839766

Family Applications (1)

Application Number Title Priority Date Filing Date
IL68733A IL68733A (en) 1982-05-19 1983-05-19 Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector

Country Status (20)

Country Link
US (2) US4657857A (da)
EP (2) EP0096430B2 (da)
JP (3) JPS59501572A (da)
AR (1) AR241796A1 (da)
AT (2) ATE42338T1 (da)
AU (1) AU571181B2 (da)
BR (1) BR8307525A (da)
CA (1) CA1224735A (da)
DE (2) DE3381090D1 (da)
DK (1) DK173699B1 (da)
ES (1) ES522556A0 (da)
FI (1) FI81379C (da)
GR (1) GR78852B (da)
HU (1) HU204097B (da)
IE (1) IE55817B1 (da)
IL (1) IL68733A (da)
NO (1) NO172397C (da)
NZ (1) NZ204302A (da)
PT (1) PT76727B (da)
WO (2) WO1983004051A1 (da)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58146281A (ja) * 1981-10-19 1983-08-31 Suntory Ltd 酵母細胞の形質転換法
US4943529A (en) 1982-05-19 1990-07-24 Gist-Brocades Nv Kluyveromyces as a host strain
AR241796A1 (es) * 1982-05-19 1992-12-30 Gist Brocades Nv Procedimiento para preparar levadura klyveromyces que contienen vectores que regulan la expresion de proteina y/o capaces de la expresion de proteinas homologas o heterologas.
JPH0716432B2 (ja) * 1982-10-20 1995-03-01 サントリー株式会社 酵母の高発現ベクタープラスミドを用いたペプチドの生産方法
CA1341116C (en) * 1983-02-22 2000-10-17 Rae Lyn Burke Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
EP0129268B1 (en) * 1983-05-19 1989-10-04 Unilever N.V. Improvements in the expression of newly introduced genes in yeast cells
WO1985001746A1 (en) * 1983-10-11 1985-04-25 Beatrice Companies, Inc. The manufacture and expression of genes for thaumatin
US6214577B1 (en) 1984-05-22 2001-04-10 Robert Rogers Yocum Yeast vectors conferring antibiotic resistance
IL75210A0 (en) * 1984-05-22 1985-09-29 Bioteknika International Yeast vector
US5422267A (en) * 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
US5240838A (en) * 1984-07-27 1993-08-31 Internationale Otrool Maatschappij "Octropa" B.V. Regulatory sequences of alcohol oxidase (MOX) and dihydroxyacetonesynthase (DAS) of Hansenula polymorpha
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US5047340A (en) * 1985-06-05 1991-09-10 The University Of Kentucky Research Foundation LAC+ saccharomyces cerevisiae
CA1310923C (en) * 1985-11-13 1992-12-01 Joachim Ludwig Weickmann Dna encoding [asp --] and [lys -, asp --] thaumatin i
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
EP0260404B1 (en) * 1986-08-21 1995-05-31 VAN DEN BERG, Robert Amylolytic enzymes producing microorganisms constructed by recombinant DNA technology and their use for fermentation processes
EP0257115A1 (en) * 1986-08-21 1988-03-02 Heineken Technisch Beheer B.V. Amylolytic enzyme producing microorganisms, constructed by recombinant DNA technology, and their use in fermentation processes
US5151354A (en) * 1986-08-21 1992-09-29 Cornelius Hollenberg Fermentation processes using amylolytic enzyme producing microorganisms
US5882884A (en) * 1987-06-19 1999-03-16 Lucky Biotech Corporation Expression of proteinaceous sweeteners in yeast
DE3885668T3 (de) * 1987-07-28 2000-08-17 Dsm Nv Kluyveromyces als Wirtsstamm.
FR2649991B2 (fr) 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
US5221624A (en) * 1988-11-08 1993-06-22 International Genetic Engineering, Inc. DNA encoding (Lys46, Asp97, Asp113) and (Lys46, Asp.sup.137) thaumatin I polypeptides
US5204252A (en) * 1989-02-08 1993-04-20 Henkel Research Corporation Candida tropicalis transformation system
ES2164633T3 (es) * 1989-02-24 2002-03-01 Monsanto Technology Llc Genes vegetales sinteticos y procedimiento para su preparacion.
US20010003849A1 (en) 1989-08-07 2001-06-14 Kenneth A. Barton Expression of genes in plants
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
FR2678636A1 (fr) * 1991-07-02 1993-01-08 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes et cellules hote utilisees.
FR2680518A1 (fr) * 1991-08-21 1993-02-26 Rhone Poulenc Rorer Sa Promoteur de levure et son utilisation.
ES2080689B1 (es) * 1994-04-21 1996-09-01 Urquima Sa Procedimiento de obtencion de un edulcorante natural proteico.
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
ES2308805T3 (es) * 1997-04-11 2008-12-01 Dsm Ip Assets B.V. Celulas de levadura que comprenden al menos dos copias de un gen deseado integradas en el genoma cromosomico en mas de un domicio que codifica el arn no ribosomico particularmente con kluyveromyces.
ES2154995B1 (es) * 1998-11-20 2001-11-16 Consejo Superior Investigacion Levadura panadera recombinante capaz de asimilar lactosa.
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
JP4637372B2 (ja) 1999-05-21 2011-02-23 カーギル ダウ エルエルシー 有機生成物の合成方法および合成材料
DE19943383B4 (de) 1999-09-10 2006-03-16 Tad Pharma Gmbh Regulatorische Sequenzen und Expressionskassetten für Hefen
CA2418777A1 (en) * 2000-08-10 2003-02-10 Ono Pharmaceutical Co., Ltd. Glycerylaldehyde-3-phosphate dehydrogenase promoter which induces apoptosis
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
BR0115558A (pt) 2000-11-22 2004-08-17 Cargill Dow Polymers Llc ácido nucleico isolado, construção de expressão recombinante, célula de levedura transformada com a construção de expressão recombinante, e, método para produzir ácido lático
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
DE60103174T2 (de) 2000-12-07 2005-05-12 Dsm Ip Assets B.V. Verfahren zur verhinderung oder verminderung der trübung in getränken
KR20030085112A (ko) 2000-12-07 2003-11-03 디에스엠 아이피 어셋츠 비.브이. 카르복시 말단 프롤린 잔기를 갖는 펩티드가 풍부한단백질 가수분해물
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
SG177002A1 (en) 2001-10-10 2012-01-30 Novo Nordisk As Remodeling and glycoconjugation of peptides
EP2305313B1 (en) 2001-10-10 2014-03-26 ratiopharm GmbH Remodelling and glycoconjugation of interferon-alpha (IFNa)
US7517671B2 (en) 2002-02-28 2009-04-14 New England Biolabs, Inc. Methods and compositions for concentrating secreted recombinant protein
BR0311655A (pt) 2002-06-07 2005-03-15 Dsm Ip Assets Bv Método melhorado para a prevenção ou redução de turbidez em bebidas
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
MXPA05010773A (es) 2003-04-09 2005-12-12 Neose Technologies Inc Metodos de glicopegilacion y proteinas/peptidos producidos por los metodos.
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
ES2755775T3 (es) 2005-11-28 2020-04-23 Dsm Ip Assets Bv Preparación de enzima que produce un sabor puro
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
CN101636489B (zh) 2007-02-20 2014-08-13 帝斯曼知识产权资产管理有限公司 新颖的唾液酸酶
EP2126060B1 (en) 2007-03-22 2016-10-05 DSM IP Assets B.V. Novel lysyl oxidases
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2011105841A2 (ko) * 2010-02-24 2011-09-01 중앙대학교 산학협력단 브라제인 발현용 재조합 발현벡터 및 높은 단맛을 가지는 신규한 브라제인 다중 변이체
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
FR2441659A1 (fr) * 1978-11-14 1980-06-13 Anvar Nouveaux plasmides hybrides et microorganismes les contenant
IL63270A0 (en) * 1980-08-05 1981-10-30 Univ Leland Stanford Junior Eukaryotic autonomously replicating segment
IL63035A (en) * 1980-09-09 1985-05-31 Univ California Dnas capable of replication and stable mitotic maintenance in a host eukaryote,their preparation and eukaryotic cells comprising them
EP0066617A1 (en) * 1980-12-10 1982-12-15 Beckman Instruments, Inc. Entraining vaporized sample in metastable gas
IE52417B1 (en) 1980-12-12 1987-10-28 Unilever Plc Dna sequences encoding various allelic forms of mature thaumatin,recombinant plasmids comprising said dnas and a process for their preparation,bacterial cultures comprising said recombinant plasmids,and method for producing mature thaumatin
EP0054331B1 (en) 1980-12-12 1992-06-03 Unilever N.V. Structural genes encoding the various allelic and maturation forms of preprothaumatin and mutations thereof, recombinant cloning vehicles comprising said structural genes and expression thereof in transformed microbial host cells
US4666847A (en) * 1981-01-16 1987-05-19 Collaborative Research, Inc. Recombinant DNA means and method
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
JPS57146571A (en) * 1981-03-09 1982-09-10 Mitsubishi Chem Ind Ltd Killer yeast
US4615974A (en) * 1981-08-25 1986-10-07 Celltech Limited Yeast expression vectors
BR8205954A (pt) 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
AR241796A1 (es) * 1982-05-19 1992-12-30 Gist Brocades Nv Procedimiento para preparar levadura klyveromyces que contienen vectores que regulan la expresion de proteina y/o capaces de la expresion de proteinas homologas o heterologas.

Also Published As

Publication number Publication date
AU571181B2 (en) 1988-04-14
DK11384A (da) 1984-01-11
AR241796A1 (es) 1992-12-30
FI81379C (fi) 1990-10-10
JPH0543348B2 (da) 1993-07-01
CA1224735A (en) 1987-07-28
GR78852B (da) 1984-10-02
ES8402874A1 (es) 1984-03-01
US4657857A (en) 1987-04-14
IE831185L (en) 1983-11-19
DE3379665D1 (en) 1989-05-24
JP2608540B2 (ja) 1997-05-07
PT76727A (en) 1983-06-01
NO172397B (no) 1993-04-05
FI840149A (fi) 1984-01-16
WO1983004051A1 (en) 1983-11-24
DK11384D0 (da) 1984-01-11
JPS59500848A (ja) 1984-05-17
JPH0685720B2 (ja) 1994-11-02
ES522556A0 (es) 1984-03-01
IE55817B1 (en) 1991-01-30
AU1553983A (en) 1983-12-02
WO1983004050A1 (en) 1983-11-24
FI840149A0 (fi) 1984-01-16
EP0096430A1 (en) 1983-12-21
ATE42338T1 (de) 1989-05-15
EP0096430B2 (en) 1998-04-01
HU204097B (en) 1991-11-28
PT76727B (en) 1985-12-27
US4859596A (en) 1989-08-22
EP0096910B1 (en) 1989-04-19
EP0096910A1 (en) 1983-12-28
NO172397C (no) 1993-07-14
FI81379B (fi) 1990-06-29
DE3381090D1 (de) 1990-02-15
EP0096430B1 (en) 1990-01-10
NZ204302A (en) 1987-03-06
BR8307525A (pt) 1984-08-21
ATE49421T1 (de) 1990-01-15
DK173699B1 (da) 2001-07-02
NO840133L (no) 1984-01-13
JPS61275227A (ja) 1986-12-05
JPS59501572A (ja) 1984-09-06

Similar Documents

Publication Publication Date Title
IL68733A (en) Kluyveromyces cells transformed by a dna sequence encoding a polypeptide,expression vectors containing said dna sequence and process for the transformation of kluyveromyces cells by said vector
ATA392082A (de) Verfahren und vorrichtung zum handhaben von gegenstaenden mittels kontinuierlich bewegter arbeitsmittel
YU87581A (en) Process for producing an improved cloning vector
IE801137L (en) Dna transfer vector
IL72642A (en) Circular dna plasmid for use as transformation vector
GB2182043B (en) Precursor polypeptide dna sequence coding therefor, vectors, host organisms and processes involving same
EG16397A (en) Reactor vessel stud closure system
DE3272083D1 (en) Reactor for reactive ion etching, and etching process
GB2024229B (en) Process for producing proteins by the expression of the corresponding adn in nbacteria dna and vectors applicable insuch processes
GB2124232B (en) An expression vector carrying a gene coding for a phosphate-binding protein
DE3477809D1 (en) Dna coding for a signal amino acid sequence and vectors containing the same
ZA873461B (en) Hybrid polypeptides comprising somatocrinine and alpha-antitrypsin,method for their production from bacterial clones and use thereof for the production of somatocrinine
GB2018778B (en) Vectors derived from lambda bacteriophage
PH18653A (en) Hybrid vector and process for improving the application and expression of hybrid vectors by the use of mitochodrial dna
PT76648A (en) Process for preparing immune modulator peptides
DE3572688D1 (en) Transformation vector for yeast yarrowia lipolytica, transformation process and transformed yeast
GB2083484B (en) Reinforced reaction injection molded elastometers
EP0207518A3 (en) Lymphotoxin dna, lymphotoxin expression vector, lymphotoxin resistant cell, transformant with lymphotoxin expression vector and process for preparing lymphotoxin
AU582416B2 (en) Polypeptide secretion-causing vector, microorganisms transformed by said vector, and process for preparing polypeptide using said microorganisms
GB8400828D0 (en) Dna expression vectors
IL72426A (en) Dna encoding a truncated hygromycin b phosphotransferase and vectors and transformants containing the dna
GR78104B (da)
DE3570248D1 (en) Use of a dna sequence for expression, and dna structure and expression vector containing said dna sequence
DE2960600D1 (en) Confinement vessel,in particular for nuclear reactor
AU9054882A (en) Process for the genetic modification of cerials with transformation vectors

Legal Events

Date Code Title Description
KB Patent renewed
HP Change in proprietorship