IL158487A0 - Method for the synthesis of dna sequences - Google Patents
Method for the synthesis of dna sequencesInfo
- Publication number
- IL158487A0 IL158487A0 IL15848702A IL15848702A IL158487A0 IL 158487 A0 IL158487 A0 IL 158487A0 IL 15848702 A IL15848702 A IL 15848702A IL 15848702 A IL15848702 A IL 15848702A IL 158487 A0 IL158487 A0 IL 158487A0
- Authority
- IL
- Israel
- Prior art keywords
- dna
- segments
- synthesis
- sequence
- substrate
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 3
- 230000015572 biosynthetic process Effects 0.000 title abstract 3
- 238000003786 synthesis reaction Methods 0.000 title abstract 3
- 108020004414 DNA Proteins 0.000 abstract 9
- 102000053602 DNA Human genes 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 238000000018 DNA microarray Methods 0.000 abstract 1
- 230000005540 biological transmission Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000002493 microarray Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
- C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00436—Maskless processes
- B01J2219/00439—Maskless processes using micromirror arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00436—Maskless processes
- B01J2219/00441—Maskless processes using lasers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00452—Means for the recovery of reactants or products
- B01J2219/00454—Means for the recovery of reactants or products by chemical cleavage from the solid support
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/0059—Sequential processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00675—In-situ synthesis on the substrate
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00686—Automatic
- B01J2219/00689—Automatic using computers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/007—Simulation or vitual synthesis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
- C07B2200/00—Indexing scheme relating to specific properties of organic compounds
- C07B2200/11—Compounds covalently bound to a solid support
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
- C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nanotechnology (AREA)
- Microbiology (AREA)
- Materials Engineering (AREA)
- General Physics & Mathematics (AREA)
- Composite Materials (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29216001P | 2001-05-18 | 2001-05-18 | |
PCT/US2002/015951 WO2002095073A1 (en) | 2001-05-18 | 2002-05-17 | Method for the synthesis of dna sequences |
Publications (1)
Publication Number | Publication Date |
---|---|
IL158487A0 true IL158487A0 (en) | 2004-05-12 |
Family
ID=23123483
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL15848702A IL158487A0 (en) | 2001-05-18 | 2002-05-17 | Method for the synthesis of dna sequences |
Country Status (7)
Country | Link |
---|---|
US (2) | US7183406B2 (de) |
EP (1) | EP1392868B2 (de) |
AT (1) | ATE403013T1 (de) |
CA (1) | CA2447240C (de) |
DE (1) | DE60227943D1 (de) |
IL (1) | IL158487A0 (de) |
WO (1) | WO2002095073A1 (de) |
Families Citing this family (86)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE60227943D1 (de) | 2001-05-18 | 2008-09-11 | Wisconsin Alumni Res Found | Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden |
KR101026816B1 (ko) * | 2002-02-28 | 2011-04-04 | 위스콘신 얼럼나이 리서어치 화운데이션 | 핵산 집단내 오류 저감 방법 |
US7452666B2 (en) | 2002-03-25 | 2008-11-18 | Lawrence Livermore National Security, Llc | Synthesis of DNA |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
US7871799B2 (en) | 2002-11-22 | 2011-01-18 | Lawrence Livermore National Security, Llc | Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions |
US7090979B2 (en) | 2002-11-22 | 2006-08-15 | The Regents Of The University Of California | Derivatized versions of ligase enzymes for constructing DNA sequences |
US7879580B2 (en) * | 2002-12-10 | 2011-02-01 | Massachusetts Institute Of Technology | Methods for high fidelity production of long nucleic acid molecules |
US20050106590A1 (en) * | 2003-05-22 | 2005-05-19 | Lathrop Richard H. | Method for producing a synthetic gene or other DNA sequence |
JP2007534303A (ja) * | 2003-05-22 | 2007-11-29 | ユニバーシティー オブ カリフォルニア | 合成遺伝子または他のdna配列を作製するための方法 |
EP1706488B1 (de) * | 2004-01-12 | 2013-07-24 | Roche NimbleGen, Inc. | Verfahren zur pcr-amplifikation auf einem microarray |
WO2005089110A2 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
CN1950519A (zh) * | 2004-02-27 | 2007-04-18 | 哈佛大学的校长及成员们 | 聚合酶群落荧光原位测序珠子 |
US20100016178A1 (en) | 2004-06-09 | 2010-01-21 | Sussman Michael R | Methods for rapid production of target double-stranded dna sequences |
CA2578564C (en) | 2004-08-27 | 2013-05-28 | Wisconsin Alumni Research Foundation | Method of error reduction in nucleic acid populations |
US20070269870A1 (en) * | 2004-10-18 | 2007-11-22 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
US7560417B2 (en) * | 2005-01-13 | 2009-07-14 | Wisconsin Alumni Research Foundation | Method and apparatus for parallel synthesis of chain molecules such as DNA |
US20060234264A1 (en) * | 2005-03-14 | 2006-10-19 | Affymetrix, Inc. | Multiplex polynucleotide synthesis |
US20070009928A1 (en) * | 2005-03-31 | 2007-01-11 | Lathrop Richard H | Gene synthesis using pooled DNA |
WO2007005053A1 (en) * | 2005-06-30 | 2007-01-11 | Codon Devices, Inc. | Hierarchical assembly methods for genome engineering |
WO2007016502A2 (en) | 2005-08-02 | 2007-02-08 | Wisconsin Alumni Research Foundation | Synthesis of arrays of oligonucleotides and other chain molecules |
US20070196834A1 (en) * | 2005-09-09 | 2007-08-23 | Francesco Cerrina | Method and system for the generation of large double stranded DNA fragments |
US20070099195A1 (en) * | 2005-11-02 | 2007-05-03 | Huang Xiaohua C | Methods and compositions for separating nucleic acids from a solid support |
US7994098B2 (en) | 2005-12-09 | 2011-08-09 | Wisconsin Alumni Research Foundation | Light directed DNA synthesis using inverse capping for error reduction |
US20070148681A1 (en) * | 2005-12-16 | 2007-06-28 | Gene Oracle, Inc. | Gene synthesis kit |
US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
US20070275399A1 (en) * | 2006-05-04 | 2007-11-29 | Lathrop Richard H | Methods for calculating codon pair-based translational kinetics values, and methods for generating polypeptide-encoding nucleotide sequences from such values |
US20090087840A1 (en) * | 2006-05-19 | 2009-04-02 | Codon Devices, Inc. | Combined extension and ligation for nucleic acid assembly |
US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2008115632A2 (en) * | 2007-02-09 | 2008-09-25 | The Regents Of The University Of California | Method for recombining dna sequences and compositions related thereto |
EP2190988A4 (de) * | 2007-08-07 | 2010-12-22 | Agency Science Tech & Res | Integrierte mikrofluidvorrichtung für die gensynthese |
JP2009268665A (ja) * | 2008-05-07 | 2009-11-19 | Canon Inc | 吸入装置 |
EP2285974A2 (de) | 2008-05-14 | 2011-02-23 | British Columbia Cancer Agency Branch | Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen |
US20100047876A1 (en) * | 2008-08-08 | 2010-02-25 | President And Fellows Of Harvard College | Hierarchical assembly of polynucleotides |
US8808986B2 (en) | 2008-08-27 | 2014-08-19 | Gen9, Inc. | Methods and devices for high fidelity polynucleotide synthesis |
US10207240B2 (en) | 2009-11-03 | 2019-02-19 | Gen9, Inc. | Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly |
EP2504449B1 (de) | 2009-11-25 | 2016-03-23 | Gen9, Inc. | Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion |
US9216414B2 (en) | 2009-11-25 | 2015-12-22 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
WO2011085075A2 (en) | 2010-01-07 | 2011-07-14 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
US8716467B2 (en) * | 2010-03-03 | 2014-05-06 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
WO2011143556A1 (en) | 2010-05-13 | 2011-11-17 | Gen9, Inc. | Methods for nucleotide sequencing and high fidelity polynucleotide synthesis |
US9187777B2 (en) | 2010-05-28 | 2015-11-17 | Gen9, Inc. | Methods and devices for in situ nucleic acid synthesis |
US9268903B2 (en) | 2010-07-06 | 2016-02-23 | Life Technologies Corporation | Systems and methods for sequence data alignment quality assessment |
US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
CA3132011A1 (en) | 2010-11-12 | 2012-06-14 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
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2002
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- 2002-05-17 EP EP02737017.0A patent/EP1392868B2/de not_active Expired - Lifetime
- 2002-05-17 CA CA2447240A patent/CA2447240C/en not_active Expired - Fee Related
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- 2002-05-17 WO PCT/US2002/015951 patent/WO2002095073A1/en not_active Application Discontinuation
- 2002-05-17 AT AT02737017T patent/ATE403013T1/de not_active IP Right Cessation
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US8008005B2 (en) | 2011-08-30 |
ATE403013T1 (de) | 2008-08-15 |
CA2447240C (en) | 2013-02-19 |
EP1392868B2 (de) | 2013-09-04 |
EP1392868A4 (de) | 2005-11-16 |
EP1392868A1 (de) | 2004-03-03 |
EP1392868B1 (de) | 2008-07-30 |
CA2447240A1 (en) | 2002-11-28 |
DE60227943D1 (de) | 2008-09-11 |
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