IL107815A - Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing - Google Patents
Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testingInfo
- Publication number
- IL107815A IL107815A IL10781593A IL10781593A IL107815A IL 107815 A IL107815 A IL 107815A IL 10781593 A IL10781593 A IL 10781593A IL 10781593 A IL10781593 A IL 10781593A IL 107815 A IL107815 A IL 107815A
- Authority
- IL
- Israel
- Prior art keywords
- stress
- pct
- methods
- genetic constructs
- responsive promoter
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL13979400A IL139794A0 (en) | 1992-12-04 | 2000-11-20 | A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US98842892A | 1992-12-04 | 1992-12-04 | |
US6317393A | 1993-05-14 | 1993-05-14 |
Publications (2)
Publication Number | Publication Date |
---|---|
IL107815A0 IL107815A0 (en) | 1994-04-12 |
IL107815A true IL107815A (en) | 2003-12-10 |
Family
ID=26743124
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL10781593A IL107815A (en) | 1992-12-04 | 1993-12-01 | Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing |
IL13979400A IL139794A0 (en) | 1992-12-04 | 2000-11-20 | A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL13979400A IL139794A0 (en) | 1992-12-04 | 2000-11-20 | A transformed bioluminescent microorganism comprising a stress-responsive promoter linked to a lux reporter operon and a tolc-mutation, which increases its bioluminescence upon environmental insult |
Country Status (11)
Country | Link |
---|---|
US (1) | US5683868A (ko) |
EP (1) | EP0673439B2 (ko) |
JP (1) | JPH08504101A (ko) |
KR (1) | KR100262681B1 (ko) |
AT (1) | ATE152181T1 (ko) |
AU (1) | AU5730494A (ko) |
CA (1) | CA2150232C (ko) |
DE (1) | DE69310207T3 (ko) |
ES (1) | ES2102811T5 (ko) |
IL (2) | IL107815A (ko) |
WO (1) | WO1994013831A1 (ko) |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69527850T2 (de) * | 1994-11-23 | 2003-04-24 | E.I. Du Pont De Nemours & Co., Inc. | Bioluminiscente lyophilisierte bakterien-reagenz zur giftstoff nachweit |
BRPI9612617B8 (pt) * | 1996-04-25 | 2021-05-25 | Vlaamse Instelling Voor Tech Onderzoek Vito | vetor recombinante, microorganismo hospedeiro geneticamente modificado, método para determinar a presença de compostos genotóxicos em uma amostra e método para determinar tanto a genotoxicidde quanto a mutagenicidade de uma amostra |
US5817463A (en) * | 1996-06-28 | 1998-10-06 | Abbott Laboratories | Nucleic acid primers and probes for detecting Mycoplasma pneumoniae |
AUPO128096A0 (en) * | 1996-07-29 | 1996-08-22 | Crc For Waste Management And Pollution Control Limited | Biosensors |
US6025131A (en) | 1996-10-23 | 2000-02-15 | E. I. Du Pont De Namours And Company | Facile method for identifying regulated promoters |
WO1998021347A1 (en) * | 1996-11-15 | 1998-05-22 | E.I. Du Pont De Nemours And Company | A small volume, highly sensitive method for detecting environmental insults |
WO1998038336A1 (en) * | 1997-02-28 | 1998-09-03 | E.I. Du Pont De Nemours And Company | A method for identifying the site of action of xenobiotic chemicals |
GB9713666D0 (en) | 1997-06-27 | 1997-09-03 | Univ Cambridge Tech | Biosensor materials and methods |
EP1023434A4 (en) * | 1997-09-18 | 2004-12-01 | Smithkline Beecham Corp | METHOD FOR SCREENING ANTIMICROBIAL COMPOUNDS |
IL137934A0 (en) * | 1998-03-06 | 2001-10-31 | Du Pont | Methods for identifying polycationic, peptide-like compounds with antibacterial activity |
US6703211B1 (en) * | 1998-03-13 | 2004-03-09 | Promega Corporation | Cellular detection by providing high energy phosphate donor other than ADP to produce ATP |
EP0950717A1 (en) * | 1998-04-14 | 1999-10-20 | "VLAAMSE INSTELLING VOOR TECHNOLOGISCH ONDERZOEK", afgekort "V.I.T.O." | Diagnostic system and method for determining the presence of a genotoxic compound in a sample |
GB9819666D0 (en) * | 1998-09-09 | 1998-11-04 | Univ Aberdeen | Biosensor |
US6720415B2 (en) * | 1998-12-02 | 2004-04-13 | Princeton University | Compositions and methods for regulating bacterial pathogenesis |
US6602666B2 (en) | 1998-12-02 | 2003-08-05 | Vlaamse Instelling Technologisch Onderzoek (V.I.T.O.) | Recombinant nucleic acid sequences and methods for determining both genotoxicity and mutagenicity of a sample and the kinetics of the genotoxicity |
US6677431B2 (en) | 1999-12-10 | 2004-01-13 | The Trustees Of The University Of Pennsylvania | Design, preparation, and properties of antibacterial β-peptides |
GB0000908D0 (en) * | 2000-01-14 | 2000-03-08 | Scient Generics Ltd | Parallel free-space optical communications |
KR100414784B1 (ko) * | 2000-01-18 | 2004-01-13 | (주)바이오니아 | 연속식 수질 독성 검사 장치 |
KR100377539B1 (ko) * | 2000-04-06 | 2003-03-26 | 광주과학기술원 | 식품내 잔류하는 농약에 대한 발광미생물을 이용한 독성탐지방법 |
US6716582B2 (en) * | 2000-04-14 | 2004-04-06 | E. I. Du Pont De Nemours And Company | Cellular arrays for the identification of altered gene expression |
KR20020041603A (ko) * | 2000-11-28 | 2002-06-03 | 박호군 | 발광유전자 luxCDABE를 포함하는 재조합플라스미드, 이 플라스미드에 의해 형질 전환된 대장균SB01(KFCC 11176) 및 이의 용도 |
CA2365791A1 (en) | 2000-12-22 | 2002-06-22 | Pfizer Products Inc. | Novel bioluminescent assays and bacterial strains useful therein |
US20040076954A1 (en) * | 2001-03-12 | 2004-04-22 | Irm, Llc | Genomics-driven high speed cellular assays, development thereof, and collections of cellular reporters |
DE10138766A1 (de) * | 2001-08-07 | 2003-02-20 | Bayer Cropscience Ag | Verfahren zum Identifizieren von makrocyclischen Polyketiden |
DE10242433A1 (de) * | 2002-09-11 | 2004-03-25 | Henkel Kgaa | DNA-Chips zur Bioprozeßkontrolle |
WO2005017198A2 (en) * | 2003-08-18 | 2005-02-24 | Aic | Surface display expression system |
WO2006018049A1 (en) * | 2004-08-18 | 2006-02-23 | Aic | Surface display expression system |
WO2006096063A1 (en) * | 2005-03-11 | 2006-09-14 | Stichting Voor De Technische Wetenschappen | Construct and method for screening, selection and identification of stress factors |
KR100697245B1 (ko) | 2005-03-28 | 2007-03-21 | 한국생명공학연구원 | 중금속 유도성 프로모터와 이의 생물공학적 활용 |
US20080268488A1 (en) * | 2005-11-10 | 2008-10-30 | Sturgess Michael A | Screening Method for Dnak Inhibitors |
KR100749493B1 (ko) | 2006-03-22 | 2007-08-14 | 한국원자력연구원 | 대장균에서 rpoS 유전자의 발현을 조절함으로서프로피온산에 대한 미생물 감수성을 증진시키는 방법 |
US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
EP2748338B1 (en) * | 2011-09-08 | 2017-04-26 | Attagene, Inc. | Systems and methods for assay of bio-contaminants in water |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4581335A (en) * | 1982-12-01 | 1986-04-08 | Texas A&M University System | Process for producing a cloned luciferase-synthesizing microorganism |
US5221623A (en) * | 1986-07-22 | 1993-06-22 | Boyce Thompson Institute For Plant Research, Inc. | Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms |
US5232833A (en) * | 1988-09-14 | 1993-08-03 | Stressgen Biotechnologies Corporation | Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants |
DE3833628A1 (de) * | 1988-10-03 | 1990-04-12 | Genlux Forschungsgesellschaft | Verfahren zum nachweis und zur identifikation toxischer substanzen mit hilfe klonierter mikroorganismen |
DE3902982A1 (de) * | 1989-02-01 | 1990-08-02 | Genlux Forschungsgesellschaft | Verfahren zum nachweis von quecksilber mit hilfe von durch quecksilber zu erhoehter biolumineszenz angeregter mikroorganismen |
ATE165394T1 (de) * | 1991-03-01 | 1998-05-15 | Vito | Fusionierte gene sowie deren verwendungzur bestimmung von metall- und xenobiotikagehalten |
JPH06509712A (ja) * | 1991-07-30 | 1994-11-02 | バイオ−テクニカル・リソーシス | 水の不純物を検出するための装置 |
ES2113546T3 (es) * | 1992-07-06 | 1998-05-01 | Harvard College | Metodos y estuches de diagnostico para determinar la toxicidad de un compuesto utilizando promotores de estres bacterianos fusionados con genes reporteros. |
-
1993
- 1993-12-01 IL IL10781593A patent/IL107815A/xx not_active IP Right Cessation
- 1993-12-02 US US08/244,376 patent/US5683868A/en not_active Expired - Lifetime
- 1993-12-02 EP EP94903321A patent/EP0673439B2/en not_active Expired - Lifetime
- 1993-12-02 ES ES94903321T patent/ES2102811T5/es not_active Expired - Lifetime
- 1993-12-02 AT AT94903321T patent/ATE152181T1/de active
- 1993-12-02 WO PCT/US1993/011527 patent/WO1994013831A1/en active IP Right Grant
- 1993-12-02 KR KR1019950702274A patent/KR100262681B1/ko not_active IP Right Cessation
- 1993-12-02 AU AU57304/94A patent/AU5730494A/en not_active Abandoned
- 1993-12-02 JP JP6514216A patent/JPH08504101A/ja active Pending
- 1993-12-02 CA CA002150232A patent/CA2150232C/en not_active Expired - Fee Related
- 1993-12-02 DE DE69310207T patent/DE69310207T3/de not_active Expired - Fee Related
-
2000
- 2000-11-20 IL IL13979400A patent/IL139794A0/xx unknown
Also Published As
Publication number | Publication date |
---|---|
CA2150232A1 (en) | 1994-06-23 |
ATE152181T1 (de) | 1997-05-15 |
WO1994013831A1 (en) | 1994-06-23 |
AU5730494A (en) | 1994-07-04 |
IL107815A0 (en) | 1994-04-12 |
DE69310207T2 (de) | 1997-08-14 |
KR100262681B1 (ko) | 2000-08-01 |
US5683868A (en) | 1997-11-04 |
ES2102811T5 (es) | 2001-10-16 |
EP0673439B2 (en) | 2001-07-04 |
DE69310207T3 (de) | 2001-11-15 |
IL139794A0 (en) | 2002-02-10 |
DE69310207D1 (de) | 1997-05-28 |
KR950704511A (ko) | 1995-11-20 |
ES2102811T3 (es) | 1997-08-01 |
CA2150232C (en) | 2000-12-19 |
EP0673439B1 (en) | 1997-04-23 |
EP0673439A1 (en) | 1995-09-27 |
JPH08504101A (ja) | 1996-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IL107815A (en) | Genetic constructs comprising a stress-responsive promoter linked to a lux reporter operon and methods of use in environmental testing | |
Rosson et al. | Autoinduction of bacterial bioluminescence in a carbon limited chemostat | |
Fernandes et al. | Sequence‐specific binding by Aspergillus nidulans AflR, a C6 zinc cluster protein regulating mycotoxin biosynthesis | |
Somero et al. | Biochemical adaptation to the environment | |
Fischer et al. | Direct response of Bradyrhizobium japonicum nifA-mediated nif gene regulation to cellular oxygen status | |
GB9211416D0 (en) | Expression of genes in transgenic plants | |
Nunn et al. | Phenotypic characterization of 10 methanol oxidation mutant classes in Methylobacterium sp. strain AM1 | |
Ulitzur et al. | Evidence for tetradecanal as the natural aldehyde in bacterial bioluminescence. | |
JPS6474992A (en) | Dna sequence, plasmid and production of lipase | |
D'Ari | The SOS system | |
FI950409A (fi) | Yhdistelmä soluja, DNA-rakenteita, vektoreita ja menetelmiä fytaattia hajoittavien entsyymien ilmentämiseksi toivotuissa suhteissa | |
WO1996039429A3 (en) | Serum protein produced exclusively in adipocytes | |
Dubnau et al. | Regulation of spo0H, an early sporulation gene in bacilli | |
AU7076394A (en) | Intracellular binding proteins and use thereof | |
MXPA98003564A (es) | Genes de des-epoxidasa de violaxantina de planta ysus metodos relacionados. | |
ATE239793T1 (de) | Gensequenzen kodierend für enzyme des flavonoid stoffwechselweges mit einer flavonoid 3'- hydroxylase aktivität une ihre anwendungen | |
Galloway et al. | Signals sufficient for rho-dependent transcription termination at trp t'span a region centered 60 base pairs upstream of the earliest 3'end point. | |
DE69214136D1 (de) | Hitzebeständige pullulanase | |
Cline et al. | Bacterial bioluminescence in vivo: control and synthesis of aldehyde factor in temperature-conditional luminescence mutants | |
IL101996A (en) | Regulation of enzyme activities by contacting them with noble gases | |
Lidstrom | Genetics of carbon metabolism in methylotrophic bacteria | |
ES2161245T3 (es) | Gen marcador. | |
Real et al. | UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster | |
TERUI | Kinetics of hydrolase production by microorganisms | |
Lappano et al. | Evidence that intracellular cGMP is involved in regulating the extracellular cAMP phosphodiesterase and its specific inhibitor in Dictyostelium discoideum |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB | Patent renewed | ||
KB | Patent renewed | ||
MM9K | Patent not in force due to non-payment of renewal fees |