ES2113546T3 - Metodos y estuches de diagnostico para determinar la toxicidad de un compuesto utilizando promotores de estres bacterianos fusionados con genes reporteros. - Google Patents

Metodos y estuches de diagnostico para determinar la toxicidad de un compuesto utilizando promotores de estres bacterianos fusionados con genes reporteros.

Info

Publication number
ES2113546T3
ES2113546T3 ES93917113T ES93917113T ES2113546T3 ES 2113546 T3 ES2113546 T3 ES 2113546T3 ES 93917113 T ES93917113 T ES 93917113T ES 93917113 T ES93917113 T ES 93917113T ES 2113546 T3 ES2113546 T3 ES 2113546T3
Authority
ES
Spain
Prior art keywords
stress
toxicity
compound
methods
diagnostic kits
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93917113T
Other languages
English (en)
Inventor
Spencer B Farr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard College
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College filed Critical Harvard College
Application granted granted Critical
Publication of ES2113546T3 publication Critical patent/ES2113546T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

ESTA INVENCION PROPORCIONA METODOS Y EQUIPOS DE DIAGNOSTICO PARA DETERMINAR LA TOXICIDAD DE UN COMPUESTO. LOS METODOS Y EQUIPOS DE DIAGNOSIS DE LA INVENCION EMPLEAN UNA PLURALIDAD DE ANFITRIONES BACTERIANOS, CADA UNO DE LOS CUALES DA COBIJO A SECUENCIAS DE DNA QUE CODIFICA UN DIFERENTE PROMOTOR DE ESTRES BACTERIANO FUSIONADO A UN GEN QUE CODIFICA UN PRODUCTO MUESTREABLE. CADA UNO DE ESTOS PROMOTORES DE ESTRES ES INDUCIDO POR EXPOSICION A UN DIFERENTE TIPO DE ESTRES CELULAR. LOS PROMOTORES DE ESTRES UTILIZADOS EN ESTA INVENCION, EN SU TOTALIDAD, COMPRENDEN AQUELLOS PROMOTORES QUE RESPONDEN A ESTRES REDOX, ESTRES DE DNA, ESTRES DE PROTEINA, ESTRES DE ENERGIA Y ESTRES DE PH. LOS METODOS Y EQUIPOS DE DIAGNOSTICO DE ESTA INVENCION PUEDE SER EMPLEADA PARA CARACTERIZAR Y CUANTIFICAR LA TOXICIDAD DE UN COMPUESTO, ASI COMO PARA IDENTIFICAR EL MECANISMO CELULAR DE SU ACCION TOXICA. ADEMAS, LOS METODOS DE ESTA INVENCION PRODUCEN INFORMACION QUE CONCIERNE A LA ACCION DE UN COMPUESTO SOBRE UN NIVEL SUBCELULAR. ESTA INFORMACION PUEDE SER UTILIZADA PARA DESIGNAR ANTITOXINAS EN COMPUESTOS QUE SE HAN DEMOSTRADO TOXICOS Y EN DROGAS ACTIVAS DE DISEÑO.
ES93917113T 1992-07-06 1993-07-06 Metodos y estuches de diagnostico para determinar la toxicidad de un compuesto utilizando promotores de estres bacterianos fusionados con genes reporteros. Expired - Lifetime ES2113546T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US91079392A 1992-07-06 1992-07-06

Publications (1)

Publication Number Publication Date
ES2113546T3 true ES2113546T3 (es) 1998-05-01

Family

ID=25429335

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93917113T Expired - Lifetime ES2113546T3 (es) 1992-07-06 1993-07-06 Metodos y estuches de diagnostico para determinar la toxicidad de un compuesto utilizando promotores de estres bacterianos fusionados con genes reporteros.

Country Status (15)

Country Link
US (2) US5589337A (es)
EP (1) EP0651825B1 (es)
JP (1) JP3456703B2 (es)
KR (1) KR100300932B1 (es)
AT (1) ATE162225T1 (es)
AU (1) AU687353B2 (es)
CA (1) CA2139667C (es)
DE (1) DE69316368T2 (es)
DK (1) DK0651825T3 (es)
ES (1) ES2113546T3 (es)
GR (1) GR3026639T3 (es)
HK (1) HK1008433A1 (es)
NO (1) NO319372B1 (es)
SG (1) SG77101A1 (es)
WO (1) WO1994001584A1 (es)

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JP2004535772A (ja) * 2000-11-21 2004-12-02 アイアールエム エルエルシー 溶解性レポーター遺伝子構造
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Also Published As

Publication number Publication date
JP3456703B2 (ja) 2003-10-14
CA2139667C (en) 2005-03-29
JPH08501930A (ja) 1996-03-05
WO1994001584A1 (en) 1994-01-20
US5585232A (en) 1996-12-17
DK0651825T3 (da) 1998-09-14
SG77101A1 (en) 2000-12-19
EP0651825B1 (en) 1998-01-14
CA2139667A1 (en) 1994-01-20
HK1008433A1 (en) 1999-05-07
DE69316368T2 (de) 1998-06-25
NO950040D0 (no) 1995-01-05
US5589337A (en) 1996-12-31
GR3026639T3 (en) 1998-07-31
AU4588493A (en) 1994-01-31
DE69316368D1 (de) 1998-02-19
AU687353B2 (en) 1998-02-26
EP0651825A1 (en) 1995-05-10
NO319372B1 (no) 2005-07-25
NO950040L (no) 1995-03-06
ATE162225T1 (de) 1998-01-15
KR100300932B1 (ko) 2001-10-22
KR950702639A (ko) 1995-07-29

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