HUP0105331A2 - Új karbonil-reduktáz, ennek génje és alkalmazásának módszere - Google Patents

Új karbonil-reduktáz, ennek génje és alkalmazásának módszere

Info

Publication number
HUP0105331A2
HUP0105331A2 HU0105331A HUP0105331A HUP0105331A2 HU P0105331 A2 HUP0105331 A2 HU P0105331A2 HU 0105331 A HU0105331 A HU 0105331A HU P0105331 A HUP0105331 A HU P0105331A HU P0105331 A2 HUP0105331 A2 HU P0105331A2
Authority
HU
Hungary
Prior art keywords
polypeptide
gene
same
chloro
butyl
Prior art date
Application number
HU0105331A
Other languages
English (en)
Inventor
Junzo Hasegawa
Noriyuki Kizaki
Yukio Yamada
Yoshihiko Yasohara
Original Assignee
Kaneka Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corporation filed Critical Kaneka Corporation
Publication of HUP0105331A2 publication Critical patent/HUP0105331A2/hu
Publication of HUP0105331A3 publication Critical patent/HUP0105331A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters

Abstract

Egy a tercier-butil (3R,5S)-6-kloro-3,5-dihidroxi-hexanoát termeléséreszolgáló, új polipeptidet, egy a polipeptidet kódoló gént, és apolipeptid alkalmazására szolgáló módszert biztosítanak. A polipeptidolyan enzimatikus aktivitással rendelkezik, hogy aszimmetrikusanredukálja a tercier-butit (S)-6-kloro-5-hidroxi-3-oxohexanoátottercier- butil (3R,5S)-6-kloro-3,5-dihidroxi-hexanoáttá. A polipeptida szekvencialistában megadott 1. számú szekvencia által képviseltaminosav szekvenciát tartalmaz, vagy olyan aminosav szekvenciát, amiehhez képest legalább egy aminosav helyettesítést, inzerciót, deléciótvagy addíciót tartalmaz. Ó
HU0105331A 1999-12-03 2000-11-24 Novel carbonyl reductase, gene thereof and method of using the same HUP0105331A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP34554199 1999-12-03

Publications (2)

Publication Number Publication Date
HUP0105331A2 true HUP0105331A2 (hu) 2002-05-29
HUP0105331A3 HUP0105331A3 (en) 2005-10-28

Family

ID=18377296

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0105331A HUP0105331A3 (en) 1999-12-03 2000-11-24 Novel carbonyl reductase, gene thereof and method of using the same

Country Status (15)

Country Link
US (1) US6645746B1 (hu)
EP (1) EP1152054B1 (hu)
JP (1) JP4510351B2 (hu)
KR (1) KR100512080B1 (hu)
AT (1) ATE291616T1 (hu)
AU (1) AU1551701A (hu)
CA (1) CA2360376C (hu)
CZ (1) CZ20012792A3 (hu)
DE (1) DE60018909T2 (hu)
ES (1) ES2240205T3 (hu)
HU (1) HUP0105331A3 (hu)
MX (1) MXPA01007858A (hu)
NO (1) NO20013801L (hu)
SI (1) SI20642A (hu)
WO (1) WO2001040450A1 (hu)

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WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
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US8426178B2 (en) 2008-08-27 2013-04-23 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
SI2329014T1 (sl) 2008-08-29 2015-01-30 Codexis, Inc. Polipeptidi ketoreduktaze za stereoselektivno produkcijo (4s)-3(5s)-5(4-fluorofenil)-5-hidroksipentanoil)-4-fenil-1,3-oksazolidin -2-ona
US8187856B2 (en) * 2008-12-18 2012-05-29 Codexis, Inc. Recombinant halohydrin dehalogenase polypeptides
EP2226386A1 (de) 2009-03-05 2010-09-08 IEP GmbH Verfahren zur stereoselektiven enzymatischen Reduktion von Ketoverbindungen
JP5683580B2 (ja) * 2009-06-22 2015-03-11 エスケー バイオファーマシューティカル カンパニー リミテッド カルバミン酸（ｒ）−１−アリール−２−テトラゾリル−エチルエステルの製造方法
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US9102959B2 (en) 2009-08-19 2015-08-11 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
US8404461B2 (en) * 2009-10-15 2013-03-26 SK Biopharmaceutical Co. Ltd. Method for preparation of carbamic acid (R)-1-aryl-2-tetrazolyl-ethyl ester
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KR101446551B1 (ko) 2013-02-26 2014-10-06 주식회사 아미노로직스 (2rs)-아미노-(3s)-히드록시-부티르산 또는 이의 유도체의 제조방법
CA2907576C (en) 2013-03-27 2021-05-04 Annikki Gmbh Method for isomerisation of glucose
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CN104328148A (zh) * 2014-11-04 2015-02-04 尚科生物医药（上海）有限公司 酶法制备(3r,5s)-6-氯-3,5-二羟基己酸叔丁酯
DK3256577T3 (da) 2015-02-10 2020-09-14 Codexis Inc Ketoreduktase polypeptider til syntesen af chirale forbindelser
CN104830921B (zh) * 2015-04-27 2019-07-02 上海工业生物技术研发中心 一种酶法制备他汀类化合物中间体的方法
CN105087685A (zh) * 2015-09-16 2015-11-25 连云港宏业化工有限公司 一种合成（3r，5s）-6-氯-3,5-二羟基己酸叔丁酯的方法
CN105087684A (zh) * 2015-09-16 2015-11-25 连云港宏业化工有限公司 一种（3r，5s）-6-氯-3,5-二羟基己酸叔丁酯的制备方法
CN106011092A (zh) * 2016-06-20 2016-10-12 苏州汉酶生物技术有限公司 一种工程化酮还原酶多肽及其用于制备孟鲁斯特中间体的方法
CN106011095B (zh) * 2016-07-27 2021-02-26 苏州汉酶生物技术有限公司 工程化酮还原酶多肽及使用其制备依替米贝中间体的方法
CN117511890A (zh) 2017-04-27 2024-02-06 科德克希思公司 酮还原酶多肽及多核苷酸
CN110914446B (zh) 2017-07-14 2024-02-06 C-乐克塔股份有限公司 酮还原酶
CN108441523A (zh) * 2018-03-22 2018-08-24 南京工业大学 （3r,5s）-6-氯-3,5,-二羟基己酸叔丁酯的制备方法
CN110387359B (zh) * 2018-04-17 2021-04-20 湖州颐盛生物科技有限公司 羰基还原酶突变体及其应用
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Also Published As

Publication number Publication date
AU1551701A (en) 2001-06-12
MXPA01007858A (es) 2003-07-14
CA2360376C (en) 2005-04-26
EP1152054A4 (en) 2002-11-20
DE60018909D1 (de) 2005-04-28
KR20020008116A (ko) 2002-01-29
NO20013801D0 (no) 2001-08-02
ATE291616T1 (de) 2005-04-15
EP1152054B1 (en) 2005-03-23
HUP0105331A3 (en) 2005-10-28
WO2001040450A1 (fr) 2001-06-07
SI20642A (sl) 2002-02-28
EP1152054A1 (en) 2001-11-07
NO20013801L (no) 2001-10-03
CZ20012792A3 (cs) 2002-03-13
DE60018909T2 (de) 2006-03-30
US6645746B1 (en) 2003-11-11
ES2240205T3 (es) 2005-10-16
CA2360376A1 (en) 2001-06-07
KR100512080B1 (ko) 2005-09-02
JP4510351B2 (ja) 2010-07-21

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