HUE028862T2 - A method for culturing cells for the preparation of compounds - Google Patents
A method for culturing cells for the preparation of compounds Download PDFInfo
- Publication number
- HUE028862T2 HUE028862T2 HUE10152393A HUE10152393A HUE028862T2 HU E028862 T2 HUE028862 T2 HU E028862T2 HU E10152393 A HUE10152393 A HU E10152393A HU E10152393 A HUE10152393 A HU E10152393A HU E028862 T2 HUE028862 T2 HU E028862T2
- Authority
- HU
- Hungary
- Prior art keywords
- glucose
- dgl
- cells
- cell
- specific
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 59
- 238000004519 manufacturing process Methods 0.000 title claims description 19
- 150000001875 compounds Chemical class 0.000 title description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 83
- 239000008103 glucose Substances 0.000 claims abstract description 82
- 239000000126 substance Substances 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 63
- 108060003951 Immunoglobulin Proteins 0.000 claims description 6
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 6
- 102000018358 immunoglobulin Human genes 0.000 claims description 6
- 229940072221 immunoglobulins Drugs 0.000 claims description 6
- 238000012423 maintenance Methods 0.000 claims description 4
- 230000004060 metabolic process Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 230000007967 glucose restriction Effects 0.000 claims 4
- 230000013595 glycosylation Effects 0.000 claims 2
- 238000006206 glycosylation reaction Methods 0.000 claims 2
- 229920001817 Agar Polymers 0.000 claims 1
- 235000016623 Fragaria vesca Nutrition 0.000 claims 1
- 240000009088 Fragaria x ananassa Species 0.000 claims 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims 1
- 240000002129 Malva sylvestris Species 0.000 claims 1
- 235000006770 Malva sylvestris Nutrition 0.000 claims 1
- 239000008272 agar Substances 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- 235000013351 cheese Nutrition 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000002431 foraging effect Effects 0.000 claims 1
- 230000035515 penetration Effects 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 239000002893 slag Substances 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 3
- 238000004321 preservation Methods 0.000 abstract 1
- 230000010412 perfusion Effects 0.000 description 12
- 230000007423 decrease Effects 0.000 description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 9
- 239000002609 medium Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 101001133081 Homo sapiens Mucin-2 Proteins 0.000 description 2
- 102100034263 Mucin-2 Human genes 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000004190 glucose uptake Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 101100290380 Caenorhabditis elegans cel-1 gene Proteins 0.000 description 1
- 241000288673 Chiroptera Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 101100452750 Ipomoea batatas IPO gene Proteins 0.000 description 1
- 101100502819 Mus musculus Fimp gene Proteins 0.000 description 1
- 206010034016 Paronychia Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000985694 Polypodiopsida Species 0.000 description 1
- 208000037656 Respiratory Sounds Diseases 0.000 description 1
- 101150001671 Tfec gene Proteins 0.000 description 1
- SGPGESCZOCHFCL-UHFFFAOYSA-N Tilisolol hydrochloride Chemical compound [Cl-].C1=CC=C2C(=O)N(C)C=C(OCC(O)C[NH2+]C(C)(C)C)C2=C1 SGPGESCZOCHFCL-UHFFFAOYSA-N 0.000 description 1
- 235000003560 Valerianella locusta Nutrition 0.000 description 1
- 240000004668 Valerianella locusta Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 210000003254 palate Anatomy 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 206010037833 rales Diseases 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10255508A DE10255508A1 (de) | 2002-11-27 | 2002-11-27 | Verfahren zur Kultivierung von Zellen zur Produktion von Substanzen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE028862T2 true HUE028862T2 (en) | 2017-01-30 |
Family
ID=32318751
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE10152393A HUE028862T2 (en) | 2002-11-27 | 2003-11-07 | A method for culturing cells for the preparation of compounds |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20060127975A1 (enExample) |
| EP (3) | EP2275530B1 (enExample) |
| JP (2) | JP4469283B2 (enExample) |
| AT (1) | ATE462013T1 (enExample) |
| CY (2) | CY1110267T1 (enExample) |
| DE (2) | DE10255508A1 (enExample) |
| DK (2) | DK2226381T3 (enExample) |
| ES (3) | ES2340377T3 (enExample) |
| HU (1) | HUE028862T2 (enExample) |
| PT (1) | PT1585810E (enExample) |
| SI (3) | SI1585810T1 (enExample) |
| WO (1) | WO2004048556A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2312184T3 (es) | 1997-03-21 | 2009-02-16 | Chugai Seiyaku Kabushiki Kaisha | Agentes preventivos terapeuticos para el tratamiento de esclerosis multiple, que contienen anticuerpos anti-receptores de il-6 antagonistas. |
| UA80091C2 (en) | 2001-04-02 | 2007-08-27 | Chugai Pharmaceutical Co Ltd | Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist |
| GB2401040A (en) * | 2003-04-28 | 2004-11-03 | Chugai Pharmaceutical Co Ltd | Method for treating interleukin-6 related diseases |
| US7429491B2 (en) * | 2003-05-15 | 2008-09-30 | Wyeth | Restricted glucose feed for animal cell culture |
| PE20091174A1 (es) | 2007-12-27 | 2009-08-03 | Chugai Pharmaceutical Co Ltd | Formulacion liquida con contenido de alta concentracion de anticuerpo |
| DE102008002210A1 (de) * | 2008-06-04 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur fermentativen Herstellung von Erythropoietin |
| CN104928336B (zh) * | 2009-10-26 | 2020-05-08 | 弗·哈夫曼-拉罗切有限公司 | 用于生产糖基化免疫球蛋白的方法 |
| AU2016210748B2 (en) * | 2009-10-26 | 2018-03-01 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| AU2015203309B2 (en) * | 2009-10-26 | 2016-05-05 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| TWI537386B (zh) * | 2009-12-22 | 2016-06-11 | Ｄｓｍ智慧財產有限公司 | 由發酵法獲得的６－胺己酸製備己內醯胺 |
| EP2787007A3 (en) | 2010-11-08 | 2015-03-11 | F. Hoffmann-La Roche AG | Subcutaneously administered ANTI-IL-6 receptor antibody |
| BR112015032960B1 (pt) | 2013-07-04 | 2021-01-05 | F. Hoffmann-La Roche Ag | imunoensaio suprimido por interferência para detectar anticorpos anti-fármaco em amostras de soro |
| EP4512889A3 (en) | 2014-03-19 | 2025-05-07 | Pfizer Inc. | Method of cell culture |
| CA3214798A1 (en) | 2015-09-23 | 2017-03-30 | Pfizer Inc. | Cells and method of cell culture |
| JP2022513702A (ja) | 2018-12-06 | 2022-02-09 | ファイザー・インク | 阻害剤産生が低下した細胞およびその使用方法 |
| WO2025088516A1 (en) | 2023-10-26 | 2025-05-01 | Pfizer Inc. | Method of forming multi-specific antibodies from homodimer antibodies and pharmaceutical product including multi-specific antibodies thus obtained |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4657863A (en) * | 1982-07-02 | 1987-04-14 | Celanese Corporation | Stabilization of a mutant microorganism population |
| JPH066054B2 (ja) * | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
| CA2078546A1 (en) * | 1990-04-03 | 1991-10-04 | Phillip W. Berman | Methods and compositions for vaccination against hiv |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| US5252216A (en) * | 1992-03-24 | 1993-10-12 | Smithkline Beecham Corporation | Protein purification |
| WO1993022448A1 (en) * | 1992-05-01 | 1993-11-11 | Teijin Limited | Fed batch culture method for protein secreting cells |
| GB9506249D0 (en) * | 1995-03-27 | 1995-05-17 | Karobio Ab | Media for insect cell cultures |
| WO1998041611A1 (en) * | 1997-03-20 | 1998-09-24 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| US6338964B1 (en) * | 1999-05-07 | 2002-01-15 | Bayer Corporation | Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration |
-
2002
- 2002-11-27 DE DE10255508A patent/DE10255508A1/de not_active Withdrawn
-
2003
- 2003-11-07 HU HUE10152393A patent/HUE028862T2/en unknown
- 2003-11-07 ES ES03767406T patent/ES2340377T3/es not_active Expired - Lifetime
- 2003-11-07 WO PCT/DE2003/003693 patent/WO2004048556A1/de not_active Ceased
- 2003-11-07 DK DK10152393.4T patent/DK2226381T3/en active
- 2003-11-07 ES ES10152393.4T patent/ES2567161T3/es not_active Expired - Lifetime
- 2003-11-07 SI SI200331791T patent/SI1585810T1/sl unknown
- 2003-11-07 EP EP10176622.8A patent/EP2275530B1/de not_active Expired - Lifetime
- 2003-11-07 ES ES10176622T patent/ES2749217T3/es not_active Expired - Lifetime
- 2003-11-07 JP JP2004554195A patent/JP4469283B2/ja not_active Expired - Lifetime
- 2003-11-07 SI SI200332473A patent/SI2226381T1/sl unknown
- 2003-11-07 EP EP10152393.4A patent/EP2226381B1/de not_active Expired - Lifetime
- 2003-11-07 PT PT03767406T patent/PT1585810E/pt unknown
- 2003-11-07 DK DK03767406.6T patent/DK1585810T3/da active
- 2003-11-07 AT AT03767406T patent/ATE462013T1/de active
- 2003-11-07 US US10/535,581 patent/US20060127975A1/en not_active Abandoned
- 2003-11-07 DE DE50312554T patent/DE50312554D1/de not_active Expired - Lifetime
- 2003-11-07 EP EP03767406A patent/EP1585810B1/de not_active Expired - Lifetime
- 2003-11-07 SI SI200332602T patent/SI2275530T1/sl unknown
-
2010
- 2010-01-19 JP JP2010009094A patent/JP5221573B2/ja not_active Expired - Lifetime
- 2010-06-11 CY CY20101100518T patent/CY1110267T1/el unknown
-
2014
- 2014-01-21 US US14/160,336 patent/US20140329277A1/en not_active Abandoned
-
2016
- 2016-05-13 CY CY20161100413T patent/CY1117558T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP4469283B2 (ja) | 2010-05-26 |
| EP1585810A1 (de) | 2005-10-19 |
| EP2275530A2 (de) | 2011-01-19 |
| SI1585810T1 (sl) | 2010-06-30 |
| EP1585810B1 (de) | 2010-03-24 |
| US20060127975A1 (en) | 2006-06-15 |
| EP2226381B1 (de) | 2016-03-09 |
| JP5221573B2 (ja) | 2013-06-26 |
| JP2010110331A (ja) | 2010-05-20 |
| WO2004048556A1 (de) | 2004-06-10 |
| CY1117558T1 (el) | 2017-04-26 |
| ATE462013T1 (de) | 2010-04-15 |
| EP2275530B1 (de) | 2019-08-07 |
| DK1585810T3 (da) | 2010-06-07 |
| ES2340377T3 (es) | 2010-06-02 |
| SI2275530T1 (sl) | 2019-11-29 |
| EP2275530A3 (de) | 2012-05-23 |
| JP2006507821A (ja) | 2006-03-09 |
| DK2226381T3 (en) | 2016-04-11 |
| DE10255508A1 (de) | 2004-06-17 |
| ES2749217T3 (es) | 2020-03-19 |
| ES2567161T3 (es) | 2016-04-20 |
| SI2226381T1 (sl) | 2016-05-31 |
| CY1110267T1 (el) | 2015-01-14 |
| US20140329277A1 (en) | 2014-11-06 |
| DE50312554D1 (de) | 2010-05-06 |
| EP2226381A1 (de) | 2010-09-08 |
| PT1585810E (pt) | 2010-05-05 |
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