PT1585810E - Processo de cultura de células para produzir substâncias - Google Patents
Processo de cultura de células para produzir substâncias Download PDFInfo
- Publication number
- PT1585810E PT1585810E PT03767406T PT03767406T PT1585810E PT 1585810 E PT1585810 E PT 1585810E PT 03767406 T PT03767406 T PT 03767406T PT 03767406 T PT03767406 T PT 03767406T PT 1585810 E PT1585810 E PT 1585810E
- Authority
- PT
- Portugal
- Prior art keywords
- glucose
- cells
- process according
- dgl
- limitation
- Prior art date
Links
- 239000000126 substance Substances 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims description 78
- 238000012258 culturing Methods 0.000 title abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 103
- 239000008103 glucose Substances 0.000 claims abstract description 102
- 210000004027 cell Anatomy 0.000 claims description 88
- 230000008569 process Effects 0.000 claims description 74
- 238000004519 manufacturing process Methods 0.000 claims description 18
- 238000012423 maintenance Methods 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 claims description 5
- 102000037865 fusion proteins Human genes 0.000 claims description 5
- 108020001507 fusion proteins Proteins 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims description 5
- 230000004060 metabolic process Effects 0.000 claims description 5
- 108090000288 Glycoproteins Proteins 0.000 claims description 4
- 102000003886 Glycoproteins Human genes 0.000 claims description 4
- 108060003951 Immunoglobulin Proteins 0.000 claims description 4
- 238000010924 continuous production Methods 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 102000018358 immunoglobulin Human genes 0.000 claims description 4
- 229940072221 immunoglobulins Drugs 0.000 claims description 4
- 210000004962 mammalian cell Anatomy 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 241000699802 Cricetulus griseus Species 0.000 claims description 2
- 102000004127 Cytokines Human genes 0.000 claims description 2
- 108090000695 Cytokines Proteins 0.000 claims description 2
- 108010092408 Eosinophil Peroxidase Proteins 0.000 claims description 2
- 102100028471 Eosinophil peroxidase Human genes 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 claims description 2
- 102000014150 Interferons Human genes 0.000 claims description 2
- 108010050904 Interferons Proteins 0.000 claims description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 2
- 238000010923 batch production Methods 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 239000003102 growth factor Substances 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- 210000004408 hybridoma Anatomy 0.000 claims description 2
- 229940047124 interferons Drugs 0.000 claims description 2
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims 1
- 235000012041 food component Nutrition 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract 1
- 229960001031 glucose Drugs 0.000 description 81
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 80
- 235000001727 glucose Nutrition 0.000 description 79
- 230000001965 increasing effect Effects 0.000 description 22
- 239000002609 medium Substances 0.000 description 18
- 230000007423 decrease Effects 0.000 description 14
- 230000010412 perfusion Effects 0.000 description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 11
- 239000000047 product Substances 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 6
- 238000001802 infusion Methods 0.000 description 6
- 230000019522 cellular metabolic process Effects 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 101001133056 Homo sapiens Mucin-1 Proteins 0.000 description 4
- 102100034256 Mucin-1 Human genes 0.000 description 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- VOZDNCFKGOLECZ-BTVCFUMJSA-N 2-hydroxypropanoic acid;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)C(O)=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O VOZDNCFKGOLECZ-BTVCFUMJSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004190 glucose uptake Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- UZOVYGYOLBIAJR-UHFFFAOYSA-N 4-isocyanato-4'-methyldiphenylmethane Chemical compound C1=CC(C)=CC=C1CC1=CC=C(N=C=O)C=C1 UZOVYGYOLBIAJR-UHFFFAOYSA-N 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 101001133081 Homo sapiens Mucin-2 Proteins 0.000 description 1
- 102100034263 Mucin-2 Human genes 0.000 description 1
- 101100346932 Mus musculus Muc1 gene Proteins 0.000 description 1
- -1 antibodies Proteins 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000012526 feed medium Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 230000005017 genetic modification Effects 0.000 description 1
- 235000013617 genetically modified food Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000037447 lactate metabolism Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10255508A DE10255508A1 (de) | 2002-11-27 | 2002-11-27 | Verfahren zur Kultivierung von Zellen zur Produktion von Substanzen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT1585810E true PT1585810E (pt) | 2010-05-05 |
Family
ID=32318751
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT03767406T PT1585810E (pt) | 2002-11-27 | 2003-11-07 | Processo de cultura de células para produzir substâncias |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20060127975A1 (enExample) |
| EP (3) | EP1585810B1 (enExample) |
| JP (2) | JP4469283B2 (enExample) |
| AT (1) | ATE462013T1 (enExample) |
| CY (2) | CY1110267T1 (enExample) |
| DE (2) | DE10255508A1 (enExample) |
| DK (2) | DK1585810T3 (enExample) |
| ES (3) | ES2749217T3 (enExample) |
| HU (1) | HUE028862T2 (enExample) |
| PT (1) | PT1585810E (enExample) |
| SI (3) | SI2226381T1 (enExample) |
| WO (1) | WO2004048556A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2284271C (en) | 1997-03-21 | 2012-05-08 | Chugai Seiyaku Kabushiki Kaisha | A preventive or therapeutic agent for sensitized t cell-mediated diseases comprising il-6 antagonist as an active ingredient |
| UA80091C2 (en) | 2001-04-02 | 2007-08-27 | Chugai Pharmaceutical Co Ltd | Remedies for infant chronic arthritis-relating diseases and still's disease which contain an interleukin-6 (il-6) antagonist |
| GB2401040A (en) | 2003-04-28 | 2004-11-03 | Chugai Pharmaceutical Co Ltd | Method for treating interleukin-6 related diseases |
| SI1623019T1 (sl) * | 2003-05-15 | 2010-10-29 | Wyeth Llc | Omejeno glukozno dovajanje za živalsko celično kulturo |
| PE20091174A1 (es) | 2007-12-27 | 2009-08-03 | Chugai Pharmaceutical Co Ltd | Formulacion liquida con contenido de alta concentracion de anticuerpo |
| DE102008002210A1 (de) * | 2008-06-04 | 2009-12-10 | Evonik Degussa Gmbh | Verfahren zur fermentativen Herstellung von Erythropoietin |
| AU2016210748B2 (en) * | 2009-10-26 | 2018-03-01 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| ES2981683T3 (es) * | 2009-10-26 | 2024-10-10 | Hoffmann La Roche | Procedimiento para la producción de una inmunoglobulina glucosilada |
| AU2015203309B2 (en) * | 2009-10-26 | 2016-05-05 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
| TWI563090B (en) * | 2009-12-22 | 2016-12-21 | Dsm Ip Assets Bv | The preparation of caprolactam from 6-amino caproic acid obtained in a fermentation process |
| KR20130086628A (ko) | 2010-11-08 | 2013-08-02 | 제넨테크, 인크. | 피하 투여용 항―ｉｌ―6 수용체 항체 |
| JP6563910B2 (ja) | 2013-07-04 | 2019-08-21 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 血清サンプル中の抗薬物抗体を検出するための干渉抑制イムノアッセイ |
| EP3119876B1 (en) | 2014-03-19 | 2024-12-18 | Pfizer Inc. | Method of cell culture |
| ES2989780T3 (es) | 2015-09-23 | 2024-11-27 | Pfizer | Células y procedimiento de cultivo celular |
| US20220073942A1 (en) | 2018-12-06 | 2022-03-10 | Pfizer Inc. | Cells with reduced inhibitor production and methods of use thereof |
| WO2025088516A1 (en) | 2023-10-26 | 2025-05-01 | Pfizer Inc. | Method of forming multi-specific antibodies from homodimer antibodies and pharmaceutical product including multi-specific antibodies thus obtained |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4657863A (en) * | 1982-07-02 | 1987-04-14 | Celanese Corporation | Stabilization of a mutant microorganism population |
| JPH066054B2 (ja) * | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
| ATE125157T1 (de) * | 1990-04-03 | 1995-08-15 | Genentech Inc | Methoden und zusammensetzungen zur impfung gegen hiv. |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| US5252216A (en) * | 1992-03-24 | 1993-10-12 | Smithkline Beecham Corporation | Protein purification |
| CA2111561A1 (en) | 1992-05-01 | 1993-11-11 | Yoshiharu Takazawa | Fed batch culture method for protein secreting cells |
| GB9506249D0 (en) * | 1995-03-27 | 1995-05-17 | Karobio Ab | Media for insect cell cultures |
| US6156570A (en) * | 1997-03-20 | 2000-12-05 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| US6338964B1 (en) * | 1999-05-07 | 2002-01-15 | Bayer Corporation | Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration |
-
2002
- 2002-11-27 DE DE10255508A patent/DE10255508A1/de not_active Withdrawn
-
2003
- 2003-11-07 WO PCT/DE2003/003693 patent/WO2004048556A1/de not_active Ceased
- 2003-11-07 JP JP2004554195A patent/JP4469283B2/ja not_active Expired - Lifetime
- 2003-11-07 HU HUE10152393A patent/HUE028862T2/en unknown
- 2003-11-07 SI SI200332473A patent/SI2226381T1/sl unknown
- 2003-11-07 SI SI200331791T patent/SI1585810T1/sl unknown
- 2003-11-07 DE DE50312554T patent/DE50312554D1/de not_active Expired - Lifetime
- 2003-11-07 AT AT03767406T patent/ATE462013T1/de active
- 2003-11-07 ES ES10176622T patent/ES2749217T3/es not_active Expired - Lifetime
- 2003-11-07 SI SI200332602T patent/SI2275530T1/sl unknown
- 2003-11-07 US US10/535,581 patent/US20060127975A1/en not_active Abandoned
- 2003-11-07 DK DK03767406.6T patent/DK1585810T3/da active
- 2003-11-07 EP EP03767406A patent/EP1585810B1/de not_active Expired - Lifetime
- 2003-11-07 ES ES10152393.4T patent/ES2567161T3/es not_active Expired - Lifetime
- 2003-11-07 PT PT03767406T patent/PT1585810E/pt unknown
- 2003-11-07 DK DK10152393.4T patent/DK2226381T3/en active
- 2003-11-07 EP EP10152393.4A patent/EP2226381B1/de not_active Expired - Lifetime
- 2003-11-07 ES ES03767406T patent/ES2340377T3/es not_active Expired - Lifetime
- 2003-11-07 EP EP10176622.8A patent/EP2275530B1/de not_active Expired - Lifetime
-
2010
- 2010-01-19 JP JP2010009094A patent/JP5221573B2/ja not_active Expired - Lifetime
- 2010-06-11 CY CY20101100518T patent/CY1110267T1/el unknown
-
2014
- 2014-01-21 US US14/160,336 patent/US20140329277A1/en not_active Abandoned
-
2016
- 2016-05-13 CY CY20161100413T patent/CY1117558T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140329277A1 (en) | 2014-11-06 |
| SI2226381T1 (sl) | 2016-05-31 |
| SI2275530T1 (sl) | 2019-11-29 |
| ES2340377T3 (es) | 2010-06-02 |
| ES2567161T3 (es) | 2016-04-20 |
| EP2275530A2 (de) | 2011-01-19 |
| CY1117558T1 (el) | 2017-04-26 |
| DE10255508A1 (de) | 2004-06-17 |
| EP2226381B1 (de) | 2016-03-09 |
| WO2004048556A1 (de) | 2004-06-10 |
| EP1585810B1 (de) | 2010-03-24 |
| EP2275530A3 (de) | 2012-05-23 |
| JP2010110331A (ja) | 2010-05-20 |
| CY1110267T1 (el) | 2015-01-14 |
| SI1585810T1 (sl) | 2010-06-30 |
| EP2275530B1 (de) | 2019-08-07 |
| EP1585810A1 (de) | 2005-10-19 |
| DK2226381T3 (en) | 2016-04-11 |
| JP2006507821A (ja) | 2006-03-09 |
| ES2749217T3 (es) | 2020-03-19 |
| HUE028862T2 (en) | 2017-01-30 |
| DK1585810T3 (da) | 2010-06-07 |
| JP5221573B2 (ja) | 2013-06-26 |
| DE50312554D1 (de) | 2010-05-06 |
| US20060127975A1 (en) | 2006-06-15 |
| ATE462013T1 (de) | 2010-04-15 |
| EP2226381A1 (de) | 2010-09-08 |
| JP4469283B2 (ja) | 2010-05-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| PT1585810E (pt) | Processo de cultura de células para produzir substâncias | |
| CN107254499B (zh) | 改进的细胞培养方法 | |
| US7390660B2 (en) | Methods for growing mammalian cells in vitro | |
| JP2011521660A (ja) | エリスロポイエチンを発酵により製造する方法 | |
| AU2011246502A1 (en) | Improved cell cultivation process | |
| IT1256566B (it) | Procedimento e apparecchiatura per controllare la concentrazione della sorgente di carbonio in una coltura aerobica di un microorganismo | |
| RU2003122076A (ru) | Способ ферментативного получения аминокислот и производных аминокислот из семейства фосфоглицератов | |
| US6680181B2 (en) | Production of peptides | |
| RU2004103494A (ru) | Способ ферментативного получения 1-метионина | |
| EP3495491A1 (en) | Method for producing recombinant protein | |
| CN103627655A (zh) | 室内生物絮团的培养方法 | |
| JP7061577B2 (ja) | Ｄ－キシロネートの製造方法およびコリネ型細菌 | |
| CN1009095B (zh) | 改进的玉米浸泡液 | |
| JP2006500045A (ja) | ファフィア・ロドジマによる供給−バッチ発酵プロセスを用いたアスタキサンチン産生 | |
| AU780122B2 (en) | Production of peptides by fedbatch cultivation of a microorganism | |
| Leegwater et al. | Aspects of microbial physiology in relation to process control | |
| CN115404222B (zh) | 一种cho细胞培养方法 | |
| CN119040246B (zh) | 一种适用于cho细胞高密度瞬时转染的培养基、方法及应用 | |
| LU601397B1 (en) | A breeding method for Micropterus salmoides | |
| RU2099423C1 (ru) | Способ получения лимонной кислоты | |
| RU2180912C1 (ru) | Способ получения дрожжей для производства игристых вин | |
| SU789066A1 (ru) | Способ культивировани планктонного рачка | |
| Chen et al. | Polypeptide production in animal cell culture | |
| Ljunggren | Metabolic control of animal cells by substrate-limited fed batch cultures. | |
| Dardenne et al. | KINETIC STUDIES OF FED-BATCH HYBRIDOMA CULTURES: EFFECT OF VARIOUS FEEDING COMPOSITIONS AND FLOW RATES |