HUE026720T2 - Xilózhasznosító zymomonas xilitol szintézis mutánsa etanol elõállítására - Google Patents

Xilózhasznosító zymomonas xilitol szintézis mutánsa etanol elõállítására Download PDF

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Publication number
HUE026720T2
HUE026720T2 HUE07874067A HUE07874067A HUE026720T2 HU E026720 T2 HUE026720 T2 HU E026720T2 HU E07874067 A HUE07874067 A HU E07874067A HU E07874067 A HUE07874067 A HU E07874067A HU E026720 T2 HUE026720 T2 HU E026720T2
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HU
Hungary
Prior art keywords
xylose
glucose
gfor
strain
gene
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HUE07874067A
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English (en)
Inventor
Paul V Viitanen
Carol M Mccutchen
Yat-Chen Chou
Min Zhang
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Du Pont
Alliance Sustainable Energy
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Publication of HUE026720T2 publication Critical patent/HUE026720T2/hu

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (5)

  1. SZABADALMI IGÉNYPONTOK Lä^kosÄite lymmemi lörgs; ätntdy igy vankmmdnáíva, hogy xilózí használjon eísnol elösOítására, xíSozmiSztadatáii gének, Siló* izomeráe, xilnlekináz, Msszkstölaz es ímtszaldeláz: bevitele által, alRd a ttelaslafew legalább egy genetikai módosítási a giuk«k-Míkiik oxldorednktax: .génen, amely esöRlteftti agkíkózslhdiiéz dxkkevdaktáz aktivitási, vagy ».hol: s giskéa-Míkísk «Ä*«dÄ£z, géft ésprexszldis. csökkentve van kosz-rmpresszióvsi, vagy a glukóz-lTXíktox »xxdoreáukiáz gért amiszenz- vagy jsserferaiö RNS Agressziójává!.
  2. 2. ,éz 1- szeri»» réRotbbloáns tywmipms törés*. Ahol a glnkbx'-tndöóz oklbempteáz: gén ;ge»$iai nKpositasa, amely esoRken» M giaMz-Srptba tAidtmsdakidz etóvkásí:, ki Wti valassitca a* kaveikezÓkbrd allé csoportból: iozereiö,: |#«^ΐ:«Βΐ|δ% feoszs^mm^ #«a*isze«s: RNA aspmssxàk
  3. 3. Ay; 1. igénypíxnt sxerínii mkombjtoáns gfotemoaax -fest, ád a genetikai módosítás, atóy csökkend glnköz-MAtóz oxidcsedukiáz aktivitási, rnzerció, amely a törzs glukóz- irnktóz oxidotednktáz génjébe be vpr homológ mkombimkaó tévén.
  4. 4. Az 1. Igénypont szerinti refeombináns: lemmas törzs:, amely törzsként vit» azormsitva, amely kr mo vdlasAva & Imvetkezokböl síié csoportból: RWbbö (ahogy Example (példa) 7-bea le van irva), ZWÍöíM· és ZWSOI-'é (ahogy Esmnplo (példa) ló-ben le van: Írva), ahol a törzsek száonaNnáimék .a törzsből EW65S, arfiély American Type Ckdture Cölíeedrm-nél ATGC » FTAsTS-Sii %%tm alatt van letétbe belyékve,
  5. 5. Az i. igénypont szerlrtir rekotnbinans Zi mv>worms törzs, ahoi a törzs csökkentett mennyiségi! M temtel összevetve genetikai módosítás óéiköií törzzsel, amely éaökketni a glukózdraktőz oxidoredekiáz aktivitást. & Az; 5. igénypont szerinti teRemhinájis: &mmmms: törzs, aired a törzs iényegébea tretn tértnél xiliioít. ?. 'tPms Zynwmonm. törzs gsnéfáráxára, amely képes sáé® hasznosítására etanol elöálitssálíim, atntiynek esökkenteti GFOE. aktivitása van, antely iaxSdmaxza: a) tendeikezésre bocsátunk rekombmáns Zmtímemrx: törzsei, amely ágy vaj; konstruálva, hogy xilözt bsszhálioa etanol előállítására alkalmas körűi rnények között a xöózláíSaMiati gének, xiiöz xzonteráz:, xilnlokináz, {nmsxkemláx és hanszakkdáz bevitele által, és b) bevezetjük a glukoz-irn kióz oxidoredukíá? gén legalább egy gene-íka: módosítását: a rekomblrutns Zymnnanm törzsbe (a)-böl, ahol a glukóz·· hulnöx oxidorsdnkiáx: pb módosítva van vagya ginköz-írukíóz oxidoreduknvz gén expressxiöja csökkentve van antíszenz RNS, nnedeniló RNS vagy kosztrprasszlö kehslxykr expsessxiója által a glnkóz'-feffeíéz oMdóredakíáz aktivitás esökkeslésére, ,1 A 7. Ígépypost azerirtii eljárás, ahol » reköiAbináns Zpmmmm törzs képes xiidz: hssznosMsára:, hogy |aj: szerlnlr elanolt állítson elő, amely M van választva a következőkből álló osoporibol: AT€€tl$2'iÄ Z. bb, £Ψ$$'$ (azÂmmém Typa Crdtare GtdNoSdmyîtei MOZ # PTA~'?|:5R szlírt alatt letétbe helyezve), 2M4(pZS5) és Z, mobilA CP4ïpZB5:. A A S, igénypont szerinti ebaz&s. ahol a gesKilikai métlosifâ» ikl van vpaszîva a következAkMi álló s&morsMl: m»«K:ié,.:Í^éc|% 'kosx^isjiaÄ;, és antiszeaz f IMS expressziö.
HUE07874067A 2006-09-28 2007-09-28 Xilózhasznosító zymomonas xilitol szintézis mutánsa etanol elõállítására HUE026720T2 (hu)

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US84781306P 2006-09-28 2006-09-28

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Country Link
US (1) US7741119B2 (hu)
EP (1) EP2066784B1 (hu)
JP (1) JP5346808B2 (hu)
CN (1) CN101861385B (hu)
BR (1) BRPI0715282A2 (hu)
CA (1) CA2661512C (hu)
ES (1) ES2554805T3 (hu)
HU (1) HUE026720T2 (hu)
PL (1) PL2066784T3 (hu)
WO (1) WO2008133638A2 (hu)

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BRPI0715282A2 (pt) 2013-07-16
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EP2066784B1 (en) 2015-09-16
CA2661512C (en) 2014-02-11
JP5346808B2 (ja) 2013-11-20
CA2661512A1 (en) 2008-11-06
CN101861385B (zh) 2013-02-20
WO2008133638A2 (en) 2008-11-06
WO2008133638A9 (en) 2008-12-31
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