HUE025068T2 - Növelt produktivitású pullulanáz változatok - Google Patents

Növelt produktivitású pullulanáz változatok Download PDF

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Publication number
HUE025068T2
HUE025068T2 HUE07837179A HUE07837179A HUE025068T2 HU E025068 T2 HUE025068 T2 HU E025068T2 HU E07837179 A HUE07837179 A HU E07837179A HU E07837179 A HUE07837179 A HU E07837179A HU E025068 T2 HUE025068 T2 HU E025068T2
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HU
Hungary
Prior art keywords
pullulanase
expression
sequence
host
bacillus
Prior art date
Application number
HUE07837179A
Other languages
English (en)
Inventor
George England
Marc Kolkman
Brian S Miller
Casper Vroemen
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of HUE025068T2 publication Critical patent/HUE025068T2/hu

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041—Pullulanase (3.2.1.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (11)

  1. SZABADALMI ίΟΕΝ^ΒΟΝΤΟΚ 1:, Izolált puífelaöáx, amely áll ^intinosav' szekvencia §E£| ID DD: 4~böl vagy SEQ ID NO: é-bol.
  2. 2. Izolált nnkMnsav molekula, amely I, igénypom szerinti pulhrDaázt kódol,
  3. 3. A 2, igénypont szerinti izolált nriklemsay molekula,, amely mrmlmazza SEí| ÍD NO; 3 vagy SLQ ID NO: 3; smkleolid: szekvenciáját.
  4. 4. Expresszié konstrukí, amely tartalmazza a 2. vagy 3. igénypont. szerinti nukleinsav molekulát.
  5. 5. A 4. igénypont szerinti express^» &»««&*. »Wl wáaaMft tsWeiosav nmlpM» van kötve a vezérlő szekwmciákhoz, amelyeket egy gazda aeft elismer, amely transzformálva es trafiszfektálva van az expresszió konstrukítal,
  6. 6. Gazda organizmus, amely tartalmazza az 5. igénypont szerinti expressziő konsteifctot.
  7. 7. A 6. igénypont szerinti gazda organizmus, ahol a gazda organizmus ki van választva a kővetkezőkből álló (Sportból: gombák, baktériumok és eukarióta sejtek,
  8. 8. A 6. igénypont szerinti gazda mganiznrus, ahol a gazda organizmus ki van választva a következőkből átló csoportból: Bacillus sp., Bacillus subtiks. Escherichia coli, Trichodenna reeset, Saeebaroittyees cerevisíaej Aspergillus ttiger ésB, ifotóiforipis,
  9. 9. Eljárás az 1, igénypont szerinti pallulanáz előállitásárá, amply íártalmazza a 6-8, igénypenfok: bárntelyike szerinti gazda organizmus tenyésztését egy ideig ss olyankörülményekkózüií,aróély alkaknas pulkiianáz e ioáliítására,
  10. 10. A ÍE igénypont szerinti eljárás* amely táriaimázzá az 5, igéitypriiK szerinti expresszié konstruiö rendelkezésére bocsátásnak és az expresszié konstraktnak a gazda organizmusba történő íraíisztorniálásának vagy tcaEBSzfektíl^Étak^^é^.
  11. 11. A 9. vagy i(>. igénypotU szerinti. eljárás, ahol a puiiuianáz izolál va van. a tenyészettől.
HUE07837179A 2006-08-23 2007-08-21 Növelt produktivitású pullulanáz változatok HUE025068T2 (hu)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83973506P 2006-08-23 2006-08-23
US90324707P 2007-02-23 2007-02-23

Publications (1)

Publication Number Publication Date
HUE025068T2 true HUE025068T2 (hu) 2016-01-28

Family

ID=38969548

Family Applications (1)

Application Number Title Priority Date Filing Date
HUE07837179A HUE025068T2 (hu) 2006-08-23 2007-08-21 Növelt produktivitású pullulanáz változatok

Country Status (15)

Country Link
US (2) US7968691B2 (hu)
EP (1) EP2054512B1 (hu)
JP (1) JP2010501181A (hu)
KR (1) KR20090041410A (hu)
CN (2) CN101506360B (hu)
BR (1) BRPI0715619A2 (hu)
CA (2) CA2663948C (hu)
CO (1) CO6160247A2 (hu)
EA (1) EA017712B1 (hu)
ES (1) ES2547857T3 (hu)
HK (1) HK1135137A1 (hu)
HU (1) HUE025068T2 (hu)
MX (1) MX2009001717A (hu)
PL (1) PL2054512T3 (hu)
WO (1) WO2008024372A2 (hu)

Families Citing this family (22)

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Publication number Priority date Publication date Assignee Title
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
CA2766975A1 (en) 2009-07-01 2011-01-06 Bayer Bioscience N.V. Methods and means for obtaining plants with enhanced glyphosate tolerance
EP3978604A1 (en) 2013-03-11 2022-04-06 Danisco US Inc. Alpha-amylase combinatorial variants
CA2915336C (en) 2013-07-17 2024-03-12 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
CN105722969A (zh) 2013-10-28 2016-06-29 丹尼斯科美国公司 大规模基因工程化活性干酵母
WO2015077126A1 (en) 2013-11-20 2015-05-28 Danisco Us Inc. Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof
AR102419A1 (es) 2014-10-23 2017-03-01 Novozymes As Variantes de glucoamilasa y polinucleótidos que las codifican
JP6770519B2 (ja) 2014-12-23 2020-10-14 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 酵素により製造されるセルロース
EP3253869A4 (en) * 2015-02-04 2018-11-07 Nanjing Bestzyme Bio-Engineering Co. Ltd. Truncated pullulanases, methods of production, and methods of use thereof
KR20170116159A (ko) 2015-02-19 2017-10-18 다니스코 유에스 인크. 향상된 단백질 발현
US11447782B2 (en) 2016-03-04 2022-09-20 Danisco Us Inc. Engineered ribosomal promoters for protein production in microorganisms
CN105925594A (zh) * 2016-06-13 2016-09-07 广西大学 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用
DK3571217T5 (da) 2017-01-23 2024-09-02 Danisco Us Inc Fremgangsmåder og sammensætninger til opnåelse af naturlig kompetence i bacillus-værtsceller
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
DK3735478T5 (da) 2018-01-03 2024-08-26 Danisco Us Inc Mutante og genmodificerede bacillus-celler og fremgangsmåder dertil til forøget proteinproduktion
WO2020172465A1 (en) * 2019-02-20 2020-08-27 Duke University Compositions and methods for the treatment of genetic diseases
CN110184258B (zh) * 2019-06-11 2020-11-06 南京林业大学 一种普鲁兰酶突变体
CN110791513B (zh) * 2019-11-15 2021-05-25 中国科学院南海海洋研究所 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用
CN111662933B (zh) * 2020-06-24 2023-03-24 江南大学 一种利用重组酵母进行高支链淀粉酒精发酵的方法
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023023642A2 (en) * 2021-08-20 2023-02-23 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells

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Also Published As

Publication number Publication date
CN101506360A (zh) 2009-08-12
CA2663948C (en) 2017-01-03
MX2009001717A (es) 2009-02-25
KR20090041410A (ko) 2009-04-28
CN103898080A (zh) 2014-07-02
CN101506360B (zh) 2014-06-11
EP2054512A2 (en) 2009-05-06
CA2949536A1 (en) 2008-02-28
EA017712B1 (ru) 2013-02-28
EA200970212A1 (ru) 2009-08-28
JP2010501181A (ja) 2010-01-21
EP2054512B1 (en) 2015-04-22
CO6160247A2 (es) 2010-05-20
WO2008024372A2 (en) 2008-02-28
US20080108126A1 (en) 2008-05-08
US7968691B2 (en) 2011-06-28
CA2663948A1 (en) 2008-02-28
ES2547857T3 (es) 2015-10-09
BRPI0715619A2 (pt) 2013-06-25
HK1135137A1 (en) 2010-05-28
WO2008024372A3 (en) 2008-04-24
US20110281326A1 (en) 2011-11-17
US8354101B2 (en) 2013-01-15
CN103898080B (zh) 2017-04-12
PL2054512T3 (pl) 2015-09-30

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