HRP20200475T1 - Postupak za razlikovanje pluripotentne matične stanice inducirane od mezenhimalne matične stanice u neuron - Google Patents
Postupak za razlikovanje pluripotentne matične stanice inducirane od mezenhimalne matične stanice u neuron Download PDFInfo
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- HRP20200475T1 HRP20200475T1 HRP20200475TT HRP20200475T HRP20200475T1 HR P20200475 T1 HRP20200475 T1 HR P20200475T1 HR P20200475T T HRP20200475T T HR P20200475TT HR P20200475 T HRP20200475 T HR P20200475T HR P20200475 T1 HRP20200475 T1 HR P20200475T1
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- Prior art keywords
- medium
- stem cells
- mem
- differentiation
- dmem
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- 238000000034 method Methods 0.000 title claims 7
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims 4
- 210000002569 neuron Anatomy 0.000 title claims 4
- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
- 239000002609 medium Substances 0.000 claims 22
- 230000004069 differentiation Effects 0.000 claims 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims 4
- 239000006147 Glasgow's Minimal Essential Medium Substances 0.000 claims 4
- 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 claims 4
- 229940018973 ecklonia cava extract Drugs 0.000 claims 4
- 239000007758 minimum essential medium Substances 0.000 claims 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims 2
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims 2
- 239000012980 RPMI-1640 medium Substances 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 239000012894 fetal calf serum Substances 0.000 claims 2
- 210000000130 stem cell Anatomy 0.000 claims 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 1
- 229930182816 L-glutamine Natural products 0.000 claims 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 claims 1
- 108010025020 Nerve Growth Factor Proteins 0.000 claims 1
- 102000007072 Nerve Growth Factors Human genes 0.000 claims 1
- 229910010252 TiO3 Inorganic materials 0.000 claims 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims 1
- 239000006143 cell culture medium Substances 0.000 claims 1
- 229910052681 coesite Inorganic materials 0.000 claims 1
- 229910052593 corundum Inorganic materials 0.000 claims 1
- 229910052906 cristobalite Inorganic materials 0.000 claims 1
- 230000032459 dedifferentiation Effects 0.000 claims 1
- 239000008367 deionised water Substances 0.000 claims 1
- 229910021641 deionized water Inorganic materials 0.000 claims 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 1
- 239000003900 neurotrophic factor Substances 0.000 claims 1
- NOTVAPJNGZMVSD-UHFFFAOYSA-N potassium monoxide Inorganic materials [K]O[K] NOTVAPJNGZMVSD-UHFFFAOYSA-N 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- 235000012239 silicon dioxide Nutrition 0.000 claims 1
- 229910052682 stishovite Inorganic materials 0.000 claims 1
- 239000013589 supplement Substances 0.000 claims 1
- 229910052905 tridymite Inorganic materials 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 229910001845 yogo sapphire Inorganic materials 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1369—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
Claims (7)
1. Metoda za diferencijaciju matičnih stanica mezenhima u neurone, koja obuhvaća korake:
(a) dodavanje ekstrakta Ecklonia cava medij stanične kulture;
(b) dediferenciranje mezenhimalnih matičnih stanica u inducirane pluripotentne matične stanice u mediju; i
(c) diferenciranje induciranih pluripotentnih matičnih stanica u neurone.
2. Postupak prema zahtjevu 1, naznačen time što se ekstrakt Ecklonia cava dodaje mediju izabranom iz skupine koja se sastoji od DMEM (Dulbeccov modificirani Eagle medij), MEM (minimalni esencijalni medij), BME (bazni Eagle medij), RPMI 1640, F- 10, F-12, DMEM-F12, α-MEM (α-minimalni esencijalni medij), G-MEM (Glasgowski minimalni esencijalni medij), IMDM (Iscove modificirani Dulbeccov medij), Mac-CoyeV 5A medij, AmnioMax kompletni medij, AminoMax II kompletni medij, Changov medij i MesemCult-XF Medium.
3. Postupak prema zahtjevu 1, naznačen time da se ekstrakt Ecklonia cava dodaje mediju u količini od 100-400 µg/ml.
4. Postupak prema zahtjevu 1, naznačen time da medij nadalje sadrži 0.01-10% v/v pročišćene deionizirane vode koja sadrži SiO2, Al2O3, TiO3, Fe2O3, CaO, Na2O, K2O i LiO.
5. Postupak prema zahtjevu 1, naznačen time da se korak c) provodi kultiviranjem induciranih pluripotentnih matičnih stanica u prvom mediju za diferencijaciju koji sadrži dodatak B-27, L-glutamin, EGF i bFGF, a zatim ih kultivira u drugom mediju za diferencijaciju koji sadrži fetalni serum teleta (FCS), bFGF i neurotrofni faktor (BDNF).
6. Metoda iz patentnog zahtjeva 5, naznačena time što je prvi i/ili drugi medij odabran iz skupine koja se sastoji od DMEM (Dulbeccov modificirani Eagle medij), MEM (minimalni esencijalni medij), BME (bazni Eagle medij), RPMI 1640, F- 10, F-12, DMEM-F12, α-MEM (α-minimalni esencijalni medij), G-MEM (Glasgow minimalni esencijalni medij), IMDM (Iscoveov modificirani Dulbeccov medij), MacCoyev 5A medij, AmnioMax kompletni medij, AminoMax II kompletni medij, Chang's Medium i Mesem-Cult-XF medij.
7. Uporaba medija pripravka koji sadrži ekstrakt Ecklonia cava za izdvajanje diferencijala mezenhimskih matičnih stanica u inducirane matične stanice pluripotencije, naznačene matičnim stanicama inducirane pluripotencije, koje se mogu diferencirati u neurone.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020130132056A KR101542847B1 (ko) | 2013-11-01 | 2013-11-01 | 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 신경세포로 분화시키는 방법 |
EP13896759.1A EP3064573B1 (en) | 2013-11-01 | 2013-11-05 | Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into neuron |
PCT/KR2013/009951 WO2015064804A1 (ko) | 2013-11-01 | 2013-11-05 | 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 신경세포로 분화시키는 방법 |
Publications (1)
Publication Number | Publication Date |
---|---|
HRP20200475T1 true HRP20200475T1 (hr) | 2020-10-02 |
Family
ID=53004392
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HRP20200475TT HRP20200475T1 (hr) | 2013-11-01 | 2020-03-20 | Postupak za razlikovanje pluripotentne matične stanice inducirane od mezenhimalne matične stanice u neuron |
Country Status (17)
Country | Link |
---|---|
US (1) | US10000735B2 (hr) |
EP (1) | EP3064573B1 (hr) |
JP (1) | JP6711759B2 (hr) |
KR (1) | KR101542847B1 (hr) |
CN (1) | CN105683360B (hr) |
AU (1) | AU2013403887B2 (hr) |
CY (1) | CY1122954T1 (hr) |
DK (1) | DK3064573T3 (hr) |
ES (1) | ES2776182T3 (hr) |
HR (1) | HRP20200475T1 (hr) |
HU (1) | HUE048803T2 (hr) |
LT (1) | LT3064573T (hr) |
PL (1) | PL3064573T3 (hr) |
PT (1) | PT3064573T (hr) |
RS (1) | RS60147B1 (hr) |
SI (1) | SI3064573T1 (hr) |
WO (1) | WO2015064804A1 (hr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2790669T3 (es) | 2013-11-01 | 2020-10-28 | Bbhc Co Ltd | Método para la producción de célula madre pluripotente inducida a partir de célula madre mesenquimatosa y célula madre pluripotente inducida producida mediante el método |
KR101699761B1 (ko) * | 2014-05-23 | 2017-01-25 | 주식회사 비비에이치씨 | 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 |
KR101592401B1 (ko) * | 2014-06-13 | 2016-02-05 | 주식회사 비비에이치씨 | 지방-유래 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포 |
KR20170024940A (ko) | 2015-08-26 | 2017-03-08 | 조선대학교산학협력단 | 신경세포로의 분화 촉진을 위한 중간엽 줄기세포의 전처리 배지 및 상기 전처리 배지를 이용한 중간엽 줄기세포의 신경세포로의 분화 촉진 방법 |
CN105907705B (zh) * | 2016-06-28 | 2020-01-24 | 广州市搏克生物技术有限公司 | 一种多能干细胞培养基 |
CN106479977B (zh) * | 2016-08-29 | 2018-02-23 | 广东依浦赛生物科技有限公司 | 人源iPS干细胞体外定向分化为神经细胞的试剂盒及方法 |
CN111004778A (zh) * | 2019-12-16 | 2020-04-14 | 江苏艾洛特生物科技有限公司 | 一种细胞无血清培养基添加物、培养基及用途 |
CN114990065A (zh) * | 2022-05-16 | 2022-09-02 | 康妍葆(北京)干细胞科技有限公司 | 神经干细胞的定向诱导剂、诱导方法及应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
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US7998460B2 (en) * | 2003-04-17 | 2011-08-16 | The Board Of Trustees Of The Leland Stanford Junior University | Prevention of deficits in neurogenesis with anti-inflammatory agents |
GB2414995B (en) * | 2004-06-09 | 2006-11-15 | Univ Edinburgh | Neural stem cells |
US20100135970A1 (en) * | 2006-10-27 | 2010-06-03 | Caritas St. Elizabeth Medical Center Of Boston, In | Methods for Reprogramming Adult Somatic Cells and Uses Thereof |
KR101016761B1 (ko) | 2009-02-26 | 2011-02-25 | 부경대학교 산학협력단 | 피부 미백활성을 갖는 감태 추출물 |
JP2012210154A (ja) * | 2009-08-03 | 2012-11-01 | Keio Gijuku | 分化細胞由来多能性幹細胞の樹立方法 |
WO2011037301A1 (ko) * | 2009-09-22 | 2011-03-31 | 서울대학교병원 | 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포 |
KR101548616B1 (ko) * | 2010-02-18 | 2015-09-01 | 연세대학교 산학협력단 | 중간엽 줄기세포를 gaβa성 신경세포로 분화시키는 방법 및 bdnf를 포함하는 gaβa성 신경세포 분화유도용 조성물 |
KR20120002134A (ko) * | 2010-06-30 | 2012-01-05 | 서울대학교산학협력단 | 지방 기질 세포의 역분화를 유도하는 방법 |
CN101914493B (zh) * | 2010-07-16 | 2012-07-04 | 山东省齐鲁干细胞工程有限公司 | 脐带间充质干细胞分化为神经细胞的诱导方法 |
KR20120040488A (ko) | 2010-10-19 | 2012-04-27 | 충남대학교산학협력단 | 곰피와 감태 추출물 유래의 플로로탄닌을 포함하는 화장품 조성물 |
KR101887956B1 (ko) | 2011-08-10 | 2018-08-13 | (주)아모레퍼시픽 | 효소 처리 감태 추출물을 포함하는 피부 미백용 조성물 |
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2013
- 2013-11-01 KR KR1020130132056A patent/KR101542847B1/ko active IP Right Grant
- 2013-11-05 DK DK13896759.1T patent/DK3064573T3/da active
- 2013-11-05 AU AU2013403887A patent/AU2013403887B2/en active Active
- 2013-11-05 RS RS20200313A patent/RS60147B1/sr unknown
- 2013-11-05 JP JP2016552369A patent/JP6711759B2/ja active Active
- 2013-11-05 US US15/033,375 patent/US10000735B2/en active Active
- 2013-11-05 WO PCT/KR2013/009951 patent/WO2015064804A1/ko active Application Filing
- 2013-11-05 CN CN201380080669.XA patent/CN105683360B/zh active Active
- 2013-11-05 ES ES13896759T patent/ES2776182T3/es active Active
- 2013-11-05 SI SI201331689T patent/SI3064573T1/sl unknown
- 2013-11-05 HU HUE13896759A patent/HUE048803T2/hu unknown
- 2013-11-05 PT PT138967591T patent/PT3064573T/pt unknown
- 2013-11-05 EP EP13896759.1A patent/EP3064573B1/en active Active
- 2013-11-05 PL PL13896759T patent/PL3064573T3/pl unknown
- 2013-11-05 LT LTEP13896759.1T patent/LT3064573T/lt unknown
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2020
- 2020-03-19 CY CY20201100260T patent/CY1122954T1/el unknown
- 2020-03-20 HR HRP20200475TT patent/HRP20200475T1/hr unknown
Also Published As
Publication number | Publication date |
---|---|
KR20150050862A (ko) | 2015-05-11 |
WO2015064804A1 (ko) | 2015-05-07 |
JP2016540521A (ja) | 2016-12-28 |
CN105683360B (zh) | 2020-12-11 |
US20160272939A1 (en) | 2016-09-22 |
EP3064573A4 (en) | 2017-09-06 |
JP6711759B2 (ja) | 2020-06-17 |
AU2013403887B2 (en) | 2018-04-05 |
CN105683360A (zh) | 2016-06-15 |
PL3064573T3 (pl) | 2020-08-10 |
SI3064573T1 (sl) | 2020-07-31 |
HUE048803T2 (hu) | 2020-08-28 |
PT3064573T (pt) | 2020-04-02 |
ES2776182T3 (es) | 2020-07-29 |
RS60147B1 (sr) | 2020-05-29 |
LT3064573T (lt) | 2020-06-10 |
EP3064573A1 (en) | 2016-09-07 |
CY1122954T1 (el) | 2021-10-29 |
US10000735B2 (en) | 2018-06-19 |
KR101542847B1 (ko) | 2015-08-10 |
AU2013403887A1 (en) | 2016-06-23 |
DK3064573T3 (da) | 2020-03-23 |
EP3064573B1 (en) | 2020-02-26 |
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