HRP20200312T1 - Metoda diferencijacije pluripotentne matične stanice izvedene iz mezenhimalne matične stanice u osteoblast - Google Patents
Metoda diferencijacije pluripotentne matične stanice izvedene iz mezenhimalne matične stanice u osteoblast Download PDFInfo
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- HRP20200312T1 HRP20200312T1 HRP20200312TT HRP20200312T HRP20200312T1 HR P20200312 T1 HRP20200312 T1 HR P20200312T1 HR P20200312T T HRP20200312T T HR P20200312TT HR P20200312 T HRP20200312 T HR P20200312T HR P20200312 T1 HRP20200312 T1 HR P20200312T1
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- Prior art keywords
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- stem cells
- mem
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- stem cell
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- 238000000034 method Methods 0.000 title claims 7
- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims 4
- 210000000963 osteoblast Anatomy 0.000 title claims 4
- 210000001778 pluripotent stem cell Anatomy 0.000 title 1
- 239000002609 medium Substances 0.000 claims 19
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims 4
- 239000006147 Glasgow's Minimal Essential Medium Substances 0.000 claims 4
- 239000007760 Iscove's Modified Dulbecco's Medium Substances 0.000 claims 4
- 229940018973 ecklonia cava extract Drugs 0.000 claims 4
- 239000007758 minimum essential medium Substances 0.000 claims 4
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims 2
- 239000012980 RPMI-1640 medium Substances 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 230000032459 dedifferentiation Effects 0.000 claims 2
- 230000004069 differentiation Effects 0.000 claims 2
- KKCBUQHMOMHUOY-UHFFFAOYSA-N Na2O Inorganic materials [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 claims 1
- 229910010252 TiO3 Inorganic materials 0.000 claims 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims 1
- 229960005070 ascorbic acid Drugs 0.000 claims 1
- 235000010323 ascorbic acid Nutrition 0.000 claims 1
- 239000011668 ascorbic acid Substances 0.000 claims 1
- DHCLVCXQIBBOPH-UHFFFAOYSA-N beta-glycerol phosphate Natural products OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 claims 1
- GHRQXJHBXKYCLZ-UHFFFAOYSA-L beta-glycerolphosphate Chemical compound [Na+].[Na+].CC(CO)OOP([O-])([O-])=O GHRQXJHBXKYCLZ-UHFFFAOYSA-L 0.000 claims 1
- 210000000988 bone and bone Anatomy 0.000 claims 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims 1
- 239000006143 cell culture medium Substances 0.000 claims 1
- 229910052681 coesite Inorganic materials 0.000 claims 1
- 229910052593 corundum Inorganic materials 0.000 claims 1
- 229910052906 cristobalite Inorganic materials 0.000 claims 1
- 239000008367 deionised water Substances 0.000 claims 1
- 229910021641 deionized water Inorganic materials 0.000 claims 1
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 claims 1
- 229960003957 dexamethasone Drugs 0.000 claims 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims 1
- 238000011177 media preparation Methods 0.000 claims 1
- 230000000921 morphogenic effect Effects 0.000 claims 1
- NOTVAPJNGZMVSD-UHFFFAOYSA-N potassium monoxide Inorganic materials [K]O[K] NOTVAPJNGZMVSD-UHFFFAOYSA-N 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- 235000012239 silicon dioxide Nutrition 0.000 claims 1
- 210000000130 stem cell Anatomy 0.000 claims 1
- 229910052682 stishovite Inorganic materials 0.000 claims 1
- 229910052905 tridymite Inorganic materials 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
- 229910001845 yogo sapphire Inorganic materials 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N2500/00—Specific components of cell culture medium
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- C12N2500/38—Vitamins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/70—Undefined extracts
- C12N2500/76—Undefined extracts from plants
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
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- C12N2501/39—Steroid hormones
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
- C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
- C12N2506/1369—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Physical Education & Sports Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (7)
1. Postupak za diferencijaciju matičnih stanica mezenhima u osteoblast, koji obuhvaća korake:
(a) dodavanje ekstrakta Ecklonia cava u medij stanične kulture;
(b) dediferenciranje mezenhimalnih matičnih stanica u inducirane pluripotentne matične stanice u mediju; i
(c) diferenciranje induciranih pluripotentnih matičnih stanica u osteoblaste.
2. Postupak sukladno zahtjevu 1, pri čemu je ekstrakt Ecklonia cava dodan u skupinu koja se sastoji od DMEM (Dulbeccov modificirani Eagle medij), MEM (minimalni esencijalni medij), BME (osnovni medij Eagle), RPMI 1640, F-10, F-12, DMEM-F12, α-MEM (α-minimalni osnovni medij), G-MEM (Glasgow minimalni osnovni medij), IMDM (Iscove modificirani Dulbecco medij), Mac-Coyev 5A medij, AmnioMax kompletni medij, AminoMax II kompletni medij, Changov medij i MesemCult-XF medij.
3. Postupak sukladno zahtjevu 1, pri čemu je ekstrakt Ecklonia cava dodan mediju u količini od 100-400 µg/ml.
4. Postupak sukladno zahtjevu 1, pri čemu medij nadalje sadrži 0.01-10 % v/v pročišćene deionizirane vode koja sadrži SiO2, Al2O3, TiO3, Fe2O3, CaO, Na2O, K2O, i LiO.
5. Postupak sukladno zahtjevu 1, pri čemu je korak c) izveden pomoću medija koji sadrži deksametazon, ß-glicerol fosfat, askorbinsku kiselinu i koštani morfogeni protein (BMP).
6. Postupak sukladno zahtjevu 5, pri čemu je medij odabran iz skupine koja se sastoji od DMEM (Dulbeccov modificirani Eagle medij), MEM (minimalni esencijalni medij), BME (osnovni medij Eagle), RPMI 1640, F-10, F-12, DMEM-F12, α-MEM (α-minimalni esencijalni medij), G-MEM (Glasgow minimalni esencijalni medij), IMDM (Iscove modificirani Dulbeccov medij), MacCoyev 5A medij, AmnioMax kompletni medij, AminoMax II kompletni medij, Changov medij i MesemCult-XF medij.
7. Uporaba pripravka medija koji obuhvaća ekstrakt Ecklonia cava za dediferencijaciju mezenhimalnih matičnih stanica u inducirane pluripotentne matične stanice, naznačena time što se matične stanice inducirane pluripotencije mogu diferencirati u osteoblaste.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020130132057A KR101542848B1 (ko) | 2013-11-01 | 2013-11-01 | 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 골아세포로 분화시키는 방법 |
| EP13896338.4A EP3064571B1 (en) | 2013-11-01 | 2013-11-05 | Method for differentiating pluripotent stem cell induced from mesenchymal stem cell into osteoblast |
| PCT/KR2013/009949 WO2015064803A1 (ko) | 2013-11-01 | 2013-11-05 | 중간엽 줄기세포로부터 유도된 만능 줄기세포를 이용하여 골아세포로 분화시키는 방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200312T1 true HRP20200312T1 (hr) | 2020-10-16 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200312TT HRP20200312T1 (hr) | 2013-11-01 | 2020-02-24 | Metoda diferencijacije pluripotentne matične stanice izvedene iz mezenhimalne matične stanice u osteoblast |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US10053669B2 (hr) |
| EP (1) | EP3064571B1 (hr) |
| JP (1) | JP6711758B2 (hr) |
| KR (1) | KR101542848B1 (hr) |
| CN (1) | CN105705631B (hr) |
| AU (1) | AU2013403886B2 (hr) |
| CY (1) | CY1122842T1 (hr) |
| DK (1) | DK3064571T3 (hr) |
| ES (1) | ES2773922T3 (hr) |
| HR (1) | HRP20200312T1 (hr) |
| HU (1) | HUE048416T2 (hr) |
| LT (1) | LT3064571T (hr) |
| PL (1) | PL3064571T3 (hr) |
| PT (1) | PT3064571T (hr) |
| RS (1) | RS60112B1 (hr) |
| SI (1) | SI3064571T1 (hr) |
| WO (1) | WO2015064803A1 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015064795A1 (ko) | 2013-11-01 | 2015-05-07 | 주식회사 비비에이치씨 | 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 및 그 방법에 의해 제조된 유도만능 줄기세포 |
| KR101699761B1 (ko) * | 2014-05-23 | 2017-01-25 | 주식회사 비비에이치씨 | 플로로탄닌 분획물을 이용한 중간엽 줄기세포로부터 유도만능 줄기세포를 제조하는 방법 |
| CN105802906A (zh) * | 2016-05-27 | 2016-07-27 | 中国医学科学院医学生物学研究所 | 树鼩骨髓间充质干细胞成骨诱导分化培养基及其制备方法与应用 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100449141B1 (ko) * | 2001-04-19 | 2004-09-21 | (주)라이프코드 | 간엽 간세포를 신경세포로 분화시키는 방법 |
| WO2006123699A1 (ja) * | 2005-05-17 | 2006-11-23 | Kyowa Hakko Kogyo Co., Ltd. | 幹細胞に対する骨芽細胞への分化促進剤 |
| US20100135970A1 (en) * | 2006-10-27 | 2010-06-03 | Caritas St. Elizabeth Medical Center Of Boston, In | Methods for Reprogramming Adult Somatic Cells and Uses Thereof |
| KR101016761B1 (ko) | 2009-02-26 | 2011-02-25 | 부경대학교 산학협력단 | 피부 미백활성을 갖는 감태 추출물 |
| JP2012210154A (ja) | 2009-08-03 | 2012-11-01 | Keio Gijuku | 分化細胞由来多能性幹細胞の樹立方法 |
| WO2011037301A1 (ko) * | 2009-09-22 | 2011-03-31 | 서울대학교병원 | 성체세포로부터 만능줄기세포를 유도하는 방법 및 그 방법에 의해 제조된 만능줄기세포 |
| JP6263329B2 (ja) | 2009-11-24 | 2018-01-17 | ザ ユニバーシティ オブ コネチカット | ヒト胚性および人工多能性幹細胞の分化 |
| KR20120002134A (ko) * | 2010-06-30 | 2012-01-05 | 서울대학교산학협력단 | 지방 기질 세포의 역분화를 유도하는 방법 |
| KR20120040488A (ko) | 2010-10-19 | 2012-04-27 | 충남대학교산학협력단 | 곰피와 감태 추출물 유래의 플로로탄닌을 포함하는 화장품 조성물 |
| KR101309538B1 (ko) * | 2010-11-17 | 2013-09-23 | 한양대학교 산학협력단 | 엑콜 화합물을 함유하는 암 줄기세포 성장 억제용 조성물 |
| KR101887956B1 (ko) | 2011-08-10 | 2018-08-13 | (주)아모레퍼시픽 | 효소 처리 감태 추출물을 포함하는 피부 미백용 조성물 |
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2013
- 2013-11-01 KR KR1020130132057A patent/KR101542848B1/ko active IP Right Grant
- 2013-11-05 DK DK13896338.4T patent/DK3064571T3/da active
- 2013-11-05 PT PT138963384T patent/PT3064571T/pt unknown
- 2013-11-05 RS RS20200233A patent/RS60112B1/sr unknown
- 2013-11-05 JP JP2016552368A patent/JP6711758B2/ja active Active
- 2013-11-05 HU HUE13896338A patent/HUE048416T2/hu unknown
- 2013-11-05 ES ES13896338T patent/ES2773922T3/es active Active
- 2013-11-05 SI SI201331670T patent/SI3064571T1/sl unknown
- 2013-11-05 LT LTEP13896338.4T patent/LT3064571T/lt unknown
- 2013-11-05 CN CN201380080661.3A patent/CN105705631B/zh active Active
- 2013-11-05 EP EP13896338.4A patent/EP3064571B1/en active Active
- 2013-11-05 WO PCT/KR2013/009949 patent/WO2015064803A1/ko active Application Filing
- 2013-11-05 PL PL13896338T patent/PL3064571T3/pl unknown
- 2013-11-05 US US15/033,393 patent/US10053669B2/en active Active
- 2013-11-05 AU AU2013403886A patent/AU2013403886B2/en active Active
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- 2020-02-21 CY CY20201100164T patent/CY1122842T1/el unknown
- 2020-02-24 HR HRP20200312TT patent/HRP20200312T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3064571A1 (en) | 2016-09-07 |
| AU2013403886B2 (en) | 2018-04-05 |
| KR20150050863A (ko) | 2015-05-11 |
| HUE048416T2 (hu) | 2020-07-28 |
| KR101542848B1 (ko) | 2015-08-10 |
| DK3064571T3 (da) | 2020-03-16 |
| WO2015064803A1 (ko) | 2015-05-07 |
| US10053669B2 (en) | 2018-08-21 |
| CN105705631B (zh) | 2021-08-03 |
| RS60112B1 (sr) | 2020-05-29 |
| EP3064571B1 (en) | 2020-01-22 |
| JP6711758B2 (ja) | 2020-06-17 |
| JP2016536019A (ja) | 2016-11-24 |
| PL3064571T3 (pl) | 2020-10-05 |
| PT3064571T (pt) | 2020-03-06 |
| CN105705631A (zh) | 2016-06-22 |
| CY1122842T1 (el) | 2021-05-05 |
| EP3064571A4 (en) | 2017-09-06 |
| LT3064571T (lt) | 2020-06-10 |
| AU2013403886A1 (en) | 2016-06-23 |
| ES2773922T3 (es) | 2020-07-15 |
| US20160257935A1 (en) | 2016-09-08 |
| SI3064571T1 (sl) | 2020-07-31 |
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