HK1215053A1 - 用作生物合成罗汉果化合物的方法及材料 - Google Patents

用作生物合成罗汉果化合物的方法及材料 Download PDF

Info

Publication number
HK1215053A1
HK1215053A1 HK16102919.6A HK16102919A HK1215053A1 HK 1215053 A1 HK1215053 A1 HK 1215053A1 HK 16102919 A HK16102919 A HK 16102919A HK 1215053 A1 HK1215053 A1 HK 1215053A1
Authority
HK
Hong Kong
Prior art keywords
methods
biosynthesis
materials
useful
mogroside compounds
Prior art date
Application number
HK16102919.6A
Other languages
English (en)
Chinese (zh)
Inventor
Yaoquan Liu
Jo̸rgen HANSEN
Jens Houghton-Larsen
Muthuswamy Panchapagesa MURALI
Sathish Kumar
Nina Nicoline RASMUSSEN
Original Assignee
Evolva Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Sa filed Critical Evolva Sa
Publication of HK1215053A1 publication Critical patent/HK1215053A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/06—Hydroxylating
    • C12P33/08—Hydroxylating at 11 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/12—Acting on D ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y303/00—Hydrolases acting on ether bonds (3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
    • C12Y504/99033—Cucurbitadienol synthase (5.4.99.33)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13132—Squalene monooxygenase (1.14.13.132)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
HK16102919.6A 2012-12-04 2013-12-04 用作生物合成罗汉果化合物的方法及材料 HK1215053A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261733220P 2012-12-04 2012-12-04
US201261733220P 2012-12-04
PCT/EP2013/075510 WO2014086842A1 (en) 2012-12-04 2013-12-04 Methods and materials for biosynthesis of mogroside compounds

Publications (1)

Publication Number Publication Date
HK1215053A1 true HK1215053A1 (zh) 2016-08-12

Family

ID=49709683

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16102919.6A HK1215053A1 (zh) 2012-12-04 2013-12-04 用作生物合成罗汉果化合物的方法及材料

Country Status (10)

Country Link
US (4) US9932619B2 (https=)
EP (1) EP2929043A1 (https=)
JP (1) JP2015536157A (https=)
AU (3) AU2015200486A1 (https=)
BR (1) BR112015013129A2 (https=)
CA (1) CA2893462A1 (https=)
HK (1) HK1215053A1 (https=)
MX (1) MX367859B (https=)
SG (2) SG10201704575XA (https=)
WO (1) WO2014086842A1 (https=)

Families Citing this family (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014012543A2 (pt) 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
EP2929043A1 (en) 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds
CA2972739A1 (en) * 2014-09-11 2016-03-17 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
CA2963300C (en) * 2014-10-01 2024-04-30 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
CA2968331C (en) * 2014-10-17 2024-02-06 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CN104988167A (zh) * 2014-11-21 2015-10-21 中国科学院天津工业生物技术研究所 罗汉果葫芦二烯醇合成酶基因SgCbQ及其应用
CN105801658B (zh) * 2014-12-30 2019-03-19 北京农学院 甜甙及其类似物的制备及作为stat3、erk信号通路靶位点药物在抗肿瘤中的应用
CN106350564A (zh) * 2015-07-20 2017-01-25 中国科学院天津工业生物技术研究所 罗汉果醇合成途径中萜类化合物及其糖基化产物的合成方法
CA3003163A1 (en) 2015-10-29 2017-05-04 Senomyx, Inc. High intensity sweeteners
CN105713060A (zh) * 2016-01-29 2016-06-29 滁州润海甜叶菊高科有限公司 一种罗汉果中提取糖分工艺
CN105713061A (zh) * 2016-03-03 2016-06-29 湖南绿蔓生物科技股份有限公司 一种罗汉果苷v含量≥95％的罗汉果提取物的制备方法
CN105777837A (zh) * 2016-05-06 2016-07-20 深圳以诺生物制药有限公司 一种新型罗汉果醇衍生物单体
WO2017198681A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
JP6605179B2 (ja) * 2017-03-01 2019-11-13 桂林莱菌生物科技股▲ふん▼有限公司 ラカンカ懸濁細胞中のモグロシドｖ含有量を高める方法
US11060124B2 (en) 2017-05-03 2021-07-13 Firmenich Incorporated Methods for making high intensity sweeteners
CA3064334A1 (en) 2017-06-02 2018-12-06 Givaudan Sa Compositions
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN109385377A (zh) * 2017-08-04 2019-02-26 中国科学院天津工业生物技术研究所 用于生产葫芦二烯醇的工程菌及其制备方法与应用
CN112041457A (zh) * 2018-02-27 2020-12-04 马努斯生物合成股份有限公司 包括罗汉果苷在内的三萜类化合物的微生物产生
JP6814182B2 (ja) * 2018-09-10 2021-01-13 アナリティコン ディスカバリー ゲーエムベーハー 甘味料または甘味増強剤として使用される新規なトリペルテングリコシド
CN109679987A (zh) * 2018-09-27 2019-04-26 怀化兴科创生物技术有限公司 一个提高甜甙含量相关基因的应用
JP2022512931A (ja) * 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
CA3118467A1 (en) * 2018-11-07 2020-05-14 Firmenich Incorporated Methods for making high intensity sweeteners
EP3877519B1 (en) 2018-11-09 2026-03-11 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
US12557831B2 (en) * 2019-02-26 2026-02-24 The Coca-Cola Company Mogrosides and uses of the same
JP7707084B2 (ja) * 2019-06-25 2025-07-14 ザ リージェンツ オブ ザ ユニヴァーシティ オブ カリフォルニア トリテルペンの製造
CN112194568B (zh) * 2019-07-08 2023-04-14 福建中医药大学 一种长链三萜类化合物及其制备方法和用途
CN110283224B (zh) * 2019-07-16 2020-08-28 湖南华诚生物资源股份有限公司 一种罗汉果甜苷v的半合成方法
WO2021126960A1 (en) * 2019-12-16 2021-06-24 Manus Bio, Inc. Microbial production of mogrol and mogrosides
MX2022011627A (es) * 2020-03-17 2022-12-15 Coca Cola Co Nuevo sistema y métodos de producción de mogrósidos.
EP4138574A1 (en) 2020-04-20 2023-03-01 Givaudan SA Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112903835B (zh) * 2021-01-12 2022-08-12 广州泽力医药科技有限公司 罗汉果橘红固体饮料的指纹图谱构建方法及检测方法
KR20240005708A (ko) * 2021-04-02 2024-01-12 징코 바이오웍스, 인크. 이소프레노이드 및 그의 전구체의 생합성
EP4314272A1 (en) * 2021-04-02 2024-02-07 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
WO2023278226A1 (en) * 2021-06-29 2023-01-05 Firmenich Incorporated Mogroside compounds and their comestible use
WO2023278976A2 (en) 2021-06-29 2023-01-05 Firmenich Incorporated Methods for making high intensity sweeteners
CN113755355A (zh) * 2021-09-30 2021-12-07 河北维达康生物科技有限公司 一种以葡萄糖为底物生物合成罗汉果醇的工程菌株、构建及其应用
TWI843509B (zh) * 2022-03-31 2024-05-21 國立臺灣大學 製備三萜類化合物的方法
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
WO2024218244A1 (en) 2023-04-20 2024-10-24 Dsm Ip Assets B.V. Strains and methods for the production of mogrosides
WO2025144795A1 (en) 2023-12-28 2025-07-03 Dsm Ip Assets B.V. Cells and methods for the production of glycosylated compounds

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US7569389B2 (en) * 2004-09-30 2009-08-04 Ceres, Inc. Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
WO2007087815A2 (en) * 2004-12-17 2007-08-09 Metanomics Gmbh Process for the control of production of fine chemicals
CA2598792A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2437573C2 (ru) 2005-11-23 2011-12-27 Дзе Кока-Кола Компани Композиция интенсивного подсластителя с кальцием и подслащенные ею композиции
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
WO2008062165A2 (en) 2006-11-22 2008-05-29 The University Of York Monoterpenoid modifying enzymes
US20100132073A1 (en) 2006-12-01 2010-05-27 The University Of York Sesquiterpenoid modifying enzymes
DE112008002456T5 (de) * 2007-09-21 2011-01-13 Basf Plant Science Gmbh Pflanzen mit erhöhtem Ertrag
GB0904639D0 (en) 2009-03-18 2009-04-29 Univ York Assay
EP2575432B1 (en) * 2010-06-02 2019-08-14 Evolva, Inc. Recombinant production of steviol glycosides
BR112014012543A2 (pt) * 2011-11-23 2017-06-13 Evolva Sa métodos e materiais para síntese enzimática de compostos de mogrosídeo
EP2929043A1 (en) * 2012-12-04 2015-10-14 Evolva SA Methods and materials for biosynthesis of mogroside compounds

Also Published As

Publication number Publication date
SG10201704575XA (en) 2017-07-28
AU2019250137B2 (en) 2022-03-24
BR112015013129A2 (pt) 2017-07-11
JP2015536157A (ja) 2015-12-21
WO2014086842A9 (en) 2015-06-18
US10662458B2 (en) 2020-05-26
AU2022204430A1 (en) 2022-07-14
AU2019250137A1 (en) 2019-10-31
EP2929043A1 (en) 2015-10-14
US9932619B2 (en) 2018-04-03
US20180251806A1 (en) 2018-09-06
AU2015200486A1 (en) 2015-03-05
SG11201503757RA (en) 2015-06-29
WO2014086842A1 (en) 2014-06-12
US10465222B2 (en) 2019-11-05
US20150322473A1 (en) 2015-11-12
US20150064743A1 (en) 2015-03-05
CA2893462A1 (en) 2014-06-12
US10011859B2 (en) 2018-07-03
US20180346953A1 (en) 2018-12-06
MX367859B (es) 2019-09-09
MX2015007039A (es) 2016-02-25

Similar Documents

Publication Publication Date Title
HK1215053A1 (zh) 用作生物合成罗汉果化合物的方法及材料
EP2670889A1 (fr) Electrolyseur et ensemble le comportant, notamment pour la production de h2 et de o2
WO2011061032A3 (de) Zellen, nukleinsäuren, enzyme und deren verwendung sowie verfahren zur herstellung von sophorolipiden
EP2675294A4 (en) Glucosyl stevia composition
PL2748300T3 (pl) Szczepy Bacillus wytwarzające enzym
WO2012159051A3 (en) Chemically modified graphene
EP2670852A4 (en) Microorganisms and methods for the biosynthesis of butadiene
MX346518B (es) Metodos de desarrollo de variantes de terpeno sintasa.
WO2013021279A3 (en) Highly galactosylated antibodies
HK1201071A1 (en) Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene
BR112013025862A2 (pt) composições de lignina, métodos de produzir as composições, métodos de utilizar composições de lignina e produtos produzidos desse modo
EP3034614A4 (en) Method for preparing rebaudioside m by using enzyme method
WO2014028429A3 (en) Enzymes and polymerases for the synthesis of rna
MX2012007710A (es) Alfa-amilasas.
WO2012058494A3 (en) Isoprene synthase variants for improved production of isoprene
WO2014172596A3 (en) Biosynthetic pathways and products
MY182946A (en) Production of organic acids by fermentation at low ph
WO2012136898A3 (en) Novel cutinases, their production and uses
EP2669353A4 (en) Antioxidant, antioxidant composition and production method therefor
WO2012104726A3 (en) Stevia sweetener with surfactant
EP2735612A4 (en) MUTANT BETA GLUCOSIDASE, ENZYME COMPOSITION FOR DECOMPOSITION OF A BIOMASS AND METHOD FOR PRODUCING A SUGAR SOLUTION
AU2012285475A8 (en) Novel IL-17R-ECD mutants
WO2012064743A3 (en) Methods for improving heart function
WO2012021678A3 (en) Isolated bacteria, methods for use, and methods for isolation
WO2011101312A3 (de) Heizanordnung