HK1099938A1 - Gene expression technique - Google Patents

Gene expression technique

Info

Publication number
HK1099938A1
HK1099938A1 HK07103159.4A HK07103159A HK1099938A1 HK 1099938 A1 HK1099938 A1 HK 1099938A1 HK 07103159 A HK07103159 A HK 07103159A HK 1099938 A1 HK1099938 A1 HK 1099938A1
Authority
HK
Hong Kong
Prior art keywords
protein
gene encoding
host cell
heterologous protein
gene expression
Prior art date
Application number
HK07103159.4A
Other languages
English (en)
Inventor
Darrell Sleep
Gillian Shuttleworth
Christopher John Arthur Finnis
Original Assignee
Novozymes Biopharma Dk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biopharma Dk As filed Critical Novozymes Biopharma Dk As
Publication of HK1099938A1 publication Critical patent/HK1099938A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK07103159.4A 2003-12-23 2007-03-23 Gene expression technique HK1099938A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0329681.1A GB0329681D0 (en) 2003-12-23 2003-12-23 Gene expression technique
PCT/GB2004/005462 WO2005061718A1 (en) 2003-12-23 2004-12-23 Gene expression technique

Publications (1)

Publication Number Publication Date
HK1099938A1 true HK1099938A1 (en) 2007-08-31

Family

ID=30776264

Family Applications (1)

Application Number Title Priority Date Filing Date
HK07103159.4A HK1099938A1 (en) 2003-12-23 2007-03-23 Gene expression technique

Country Status (16)

Country Link
US (3) US20070275889A1 (da)
EP (2) EP2048236B1 (da)
JP (1) JP5052899B2 (da)
KR (1) KR101262682B1 (da)
CN (2) CN1922325B (da)
AT (1) ATE421994T1 (da)
AU (2) AU2004303601C1 (da)
CA (1) CA2551503A1 (da)
DE (1) DE602004019304D1 (da)
DK (1) DK2048236T3 (da)
ES (1) ES2321948T3 (da)
GB (2) GB0329681D0 (da)
HK (1) HK1099938A1 (da)
SG (2) SG182155A1 (da)
WO (1) WO2005061718A1 (da)
ZA (1) ZA200605588B (da)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110014661A (ko) * 2002-02-07 2011-02-11 노보자임스 바이오파마 디케이 에이/에스 알부민-융합 쿠니츠 도메인 펩타이드
GB0329722D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
JP5631533B2 (ja) * 2004-12-23 2014-11-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 遺伝子発現技術
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
CN101511868B (zh) 2006-07-24 2013-03-06 比奥雷克西斯制药公司 毒蜥外泌肽融合蛋白
GB0711424D0 (en) * 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
CA2695830A1 (en) 2007-08-08 2009-02-12 Novozymes Biopharma Dk A/S Transferrin variants and conjugates
WO2009057813A1 (ja) 2007-10-31 2009-05-07 National Institute Of Advanced Industrial Science And Technology タンパク質の高分泌生産方法
FI3581650T3 (fi) 2008-09-15 2023-03-23 Uniqure Biopharma B V IX-tekijän polypeptidimutantti, sen käyttöjä ja menetelmä sen valmistamiseksi
WO2010034708A1 (en) * 2008-09-23 2010-04-01 Unilever Plc A polynucleotide expression cassette
JP5936112B2 (ja) 2009-02-11 2016-06-15 アルブミディクス アクティーゼルスカブ アルブミン変異体及び複合体
WO2010128142A1 (en) 2009-05-07 2010-11-11 Novozymes Biopharma Dk A/S Method for purifying albumin
EP2258854A1 (en) 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
FI20095615A0 (fi) * 2009-06-02 2009-06-02 Oulun Yliopisto Menetelmä luonnollisesti laskostuneiden proteiinien tuottamiseksi prokaryootti-isännässä
EP2493921B1 (en) 2009-10-30 2018-09-26 Albumedix Ltd Albumin variants
CN103641896B (zh) * 2009-11-19 2015-08-05 浙江大学 明胶样单元的用途
GB201000587D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
KR20130070576A (ko) 2010-04-09 2013-06-27 노보자임스 바이오파마 디케이 에이/에스 알부민 유도체 및 변이체
CN102533839B (zh) * 2010-12-21 2017-06-20 上海开阳生物科技有限公司 融合表达含有二硫键蛋白质的方法
EP2565262A1 (en) * 2011-08-31 2013-03-06 VTU Holding GmbH Protein expression
US20140315817A1 (en) 2011-11-18 2014-10-23 Eleven Biotherapeutics, Inc. Variant serum albumin with improved half-life and other properties
PL2825556T3 (pl) 2012-03-16 2018-10-31 Albumedix A/S Warianty albuminy
GB2512156A (en) 2012-11-08 2014-09-24 Novozymes Biopharma Dk As Albumin variants
CN102994541B (zh) * 2012-12-19 2015-04-15 江南大学 一种共表达upr关键基因和下游靶基因增强葡萄糖氧化酶分泌的方法
CN104937599B (zh) * 2013-02-28 2018-01-23 株式会社日立高新技术 数据解析装置及其方法
CN103436516B (zh) * 2013-08-23 2015-04-22 华南理工大学 利用大肠杆菌原核表达系统生产的小麦蛋白质二硫键异构酶及方法和应用
EP4311857A3 (en) 2014-04-17 2024-07-31 Boehringer Ingelheim RCV GmbH & Co KG Recombinant host cell engineered to overexpress helper proteins
CN106604986B (zh) 2014-04-17 2021-01-12 贝林格尔·英格海姆Rcv两合公司 用于表达目标蛋白的重组宿主细胞
EP3337816B1 (en) 2015-08-20 2024-02-14 Albumedix Ltd Albumin variants and conjugates
WO2017112847A1 (en) 2015-12-22 2017-06-29 Albumedix A/S Improved protein expression strains
WO2017190671A1 (zh) * 2016-05-04 2017-11-09 北京普罗吉生物科技发展有限公司 高效表达重组人血清白蛋白工程菌的构建
CN106834322B (zh) * 2017-03-14 2020-09-04 中国科学院南海海洋研究所 抗毒素so_1445或其编码基因在提高质粒在宿主细胞中的稳定性中的应用
BR112019027397A2 (pt) 2017-06-20 2020-07-14 Albumedix Ltd cepas de expressão de proteína melhoradas
US20210155674A1 (en) * 2017-06-30 2021-05-27 Western University Of Health Sciences Factor ix-transferrin fusion proteins
CN112004931A (zh) 2018-03-26 2020-11-27 诺维信公司 真菌伴侣蛋白
CN112391402B (zh) * 2020-11-17 2023-03-28 华中科技大学 一种提高解脂耶氏酵母中目的蛋白表达水平的方法
US20240150807A1 (en) * 2021-03-10 2024-05-09 Sumitomo Pharma Co., Ltd. Method for Producing Cysteine Knot Protein
CN114410496B (zh) * 2022-02-16 2023-10-03 江南大学 一种提高毕赤酵母外源蛋白产量的方法
CN117903295B (zh) * 2024-03-19 2024-05-31 北京国科星联科技有限公司 一种用于分泌表达乳铁蛋白的马克斯克鲁维酵母及其构建方法与应用

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9019919D0 (en) 1990-09-12 1990-10-24 Delta Biotechnology Ltd Purification of proteins
US4302386A (en) 1978-08-25 1981-11-24 The Ohio State University Antigenic modification of polypeptides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
IL66614A (en) 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
CA1205935A (en) 1981-09-14 1986-06-10 Union Carbide Corporation Process for producing a polymer water-in-oil emulsion
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
GB8620926D0 (en) 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
JP2873012B2 (ja) * 1987-04-09 1999-03-24 デルタ バイオテクノロジー リミテッド 酵母ベクター
IL86455A0 (en) 1987-06-01 1988-11-15 Takeda Chemical Industries Ltd Polypeptide and production thereof
IL88326A (en) 1987-11-18 1993-03-15 Gist Brocades Nv Purification of serum albumin
JP3092811B2 (ja) 1988-07-23 2000-09-25 デルタ バイオテクノロジー リミテッド ペプチドおよびdna配列
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
US5759802A (en) 1988-10-26 1998-06-02 Tonen Corporation Production of human serum alubumin A
GB8909916D0 (en) 1989-04-29 1989-06-14 Delta Biotechnology Ltd Polypeptides
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms
GB8927480D0 (en) 1989-12-05 1990-02-07 Delta Biotechnology Ltd Mutant fungal strain detection and new promoter
JP3230091B2 (ja) 1990-06-25 2001-11-19 ウェルファイド株式会社 ヒト血清アルブミンの着色抑制方法
JPH05508547A (ja) * 1990-07-13 1993-12-02 トランスカリオティック セラピーズ,インコーポレイテッド ライブラリースクリーニング法
EP0509841A3 (en) 1991-04-18 1993-08-18 Tonen Corporation Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
US6291205B1 (en) 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
AU4571993A (en) * 1992-07-29 1994-03-03 Unilever Plc Process for producing anti-freeze peptides
DE4244915C2 (de) 1992-08-14 1998-12-03 Widmar Prof Dr Tanner DNA-Moleküle codierend Proteine, die an der O-Glykosylierung von Proteinen beteiligt sind
US5728553A (en) 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
DE69329202T2 (de) * 1992-10-02 2001-03-29 Research Corp. Technologies, Inc. Methoden der erhöhung der sekretion von überexpremierten proteinen
GB9404270D0 (en) 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
GB9411356D0 (en) * 1994-06-07 1994-07-27 Delta Biotechnology Ltd Yeast strains
JP3945866B2 (ja) * 1996-09-04 2007-07-18 サントリー株式会社 メタノール酵母由来のプロテインジスルフィドイソメラーゼ遺伝子
WO1999013093A1 (en) 1997-09-05 1999-03-18 Pentapharm Ag Expression vector for improved production of polypeptides in yeast
CN1111602C (zh) * 1998-05-15 2003-06-18 中国科学院上海生物化学研究所 一种使用分子伴侣促进蛋白质分泌的方法
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
EP1077263A1 (de) * 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
EP1278544A4 (en) 2000-04-12 2004-08-18 Human Genome Sciences Inc ALBUMIN FUSION PROTEINS
US6358733B1 (en) * 2000-05-19 2002-03-19 Apolife, Inc. Expression of heterologous multi-domain proteins in yeast
DE10121235A1 (de) * 2001-04-30 2002-10-31 Roche Diagnostics Gmbh Verfahren zur Expression von Proteinen in in vitro Translations-Systemen unter Ko-Expression von Faltungshelferproteinen
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
US20030226155A1 (en) 2001-08-30 2003-12-04 Biorexis Pharmaceutical Corporation Modified transferrin-antibody fusion proteins
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
WO2003057897A2 (en) * 2002-01-07 2003-07-17 European Molecular Biology Laboratory Recombinant protein expression
KR20110014661A (ko) 2002-02-07 2011-02-11 노보자임스 바이오파마 디케이 에이/에스 알부민-융합 쿠니츠 도메인 펩타이드
JP2005534330A (ja) 2002-08-07 2005-11-17 デルタ バイオテクノロジー リミテッド アルブミン融合繊毛様神経栄養因子
US6939676B2 (en) * 2002-12-30 2005-09-06 The United States Of America As Represented By The Secretary Of Argriculture Selection procedure for identifying transgenic cells, embryos, and plants without the use of antibiotics
US7244616B2 (en) * 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
US7226781B1 (en) * 2003-07-24 2007-06-05 Belyaev Alexander S Chaperone expression genomes
CN101031655A (zh) * 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法

Also Published As

Publication number Publication date
AU2004303601A1 (en) 2005-07-07
ES2321948T3 (es) 2009-06-15
JP5052899B2 (ja) 2012-10-17
EP2048236A1 (en) 2009-04-15
CN101818121A (zh) 2010-09-01
ZA200605588B (en) 2009-02-25
US20120231503A1 (en) 2012-09-13
GB2424889A (en) 2006-10-11
DE602004019304D1 (de) 2009-03-19
US20070275889A1 (en) 2007-11-29
US20180105818A1 (en) 2018-04-19
EP1709181A1 (en) 2006-10-11
SG182155A1 (en) 2012-07-30
ATE421994T1 (de) 2009-02-15
GB0329681D0 (en) 2004-01-28
DK2048236T3 (da) 2013-05-21
SG149003A1 (en) 2009-01-29
CA2551503A1 (en) 2005-07-07
EP2048236B1 (en) 2013-03-20
CN1922325A (zh) 2007-02-28
KR101262682B1 (ko) 2013-05-15
JP2007515962A (ja) 2007-06-21
AU2008202538A1 (en) 2008-06-26
KR20070005568A (ko) 2007-01-10
WO2005061718A1 (en) 2005-07-07
CN1922325B (zh) 2010-06-09
AU2004303601C1 (en) 2009-01-08
AU2004303601B2 (en) 2008-03-06
GB0614614D0 (en) 2006-08-30
EP1709181B1 (en) 2009-01-28

Similar Documents

Publication Publication Date Title
HK1099938A1 (en) Gene expression technique
EP2330200A3 (en) Gene expression technique
EP1824977A4 (en) NOVEL PROMOTER NUCLEIC ACID FROM CORYNEBACTERIUM GENE BACTERIUM, EXPRESSION CASSETTE CONTAINING THE PROMOTER AND VECTOR CONTAINING THE CASSETTE, HOST CELL COMPRISING THE VECTOR, AND METHOD FOR EXPRESSION OF A GENE USING THE CELL
WO2003012058A3 (en) Media and methods for cultivation of microorganisms
AU4200897A (en) Novel host cells and methods of producing proteins
ATE405586T1 (de) Verfahren zur regulierung der immunfunktion in primaten unter verwendung des foxp3-proteins
ATE245701T1 (de) Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte
BRPI0515748A (pt) proteìna recombinante produzida por bactérias gram-positivas, método para fabricá-la, para isolar ìons de metal de um lìquido, para converter um substrato em um produto, seu uso e respectivo kit
IL155482A (en) Method for increasing product yield of a mammalian polypeptide produced by recombinant bacterial host cells
WO2002014468A3 (en) Phage-dependent superproduction of biologically active protein and peptides
AU2003238717A1 (en) POLYPEPTIDES HAVING Alpha- H-Alpha-AMINO ACID AMIDE RACEMASE ACTIVITY AND NUCLEIC ACIDS ENCODING THE SAME
DE60225312D1 (de) Samenspezifische 7s-alpha-promoter zur expression von genen in pflanzen
ATE555194T1 (de) Methode zur herstellung von proliferierenden und differenzierenden zelllinien
GB0321100D0 (en) Biological products
ATE486935T1 (de) Mikroorganismenstamm zur produktion von rekombinanten proteinen
EP1870454A4 (en) PROCESS FOR DIPEPTIDE PRODUCTION
AU2002214085A1 (en) Method for producing proteins by electroporation
DK1639119T3 (da) Fremgangsmåde til mikrobiologisk isomerisering af alpha-hydroxycarboxylsyrer
EP1262551A3 (en) A sorbitol dehydrogenase gene, a recombinant DNA, and a process for producing sorbitol dehydrogenase
NZ515762A (en) Method of producing a heparin-binding protein by expressing it in a mammalian cell that can be cultured in anaerobic conditions
WO2004027043A3 (en) Method for production of a multimeric protein by cell fusion
WO2003012098A1 (fr) Levure modifiee presentant un potentiel secretoire eleve de proteine etrangere et procede permettant de produire une proteine etrangere a l'aide de la levure
MX2023015460A (es) Celula huesped de bacillus mejorada con proteina rema/remb alterada.
CY1116010T1 (el) Διαδικασια για παραγωγη πολυπεπτιδιων
EP1561810A3 (en) Mutant microorganism and method for producing peptide using the same

Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20191223