GB2441718B - Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells - Google Patents

Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells

Info

Publication number
GB2441718B
GB2441718B GB0800264A GB0800264A GB2441718B GB 2441718 B GB2441718 B GB 2441718B GB 0800264 A GB0800264 A GB 0800264A GB 0800264 A GB0800264 A GB 0800264A GB 2441718 B GB2441718 B GB 2441718B
Authority
GB
United Kingdom
Prior art keywords
cells
cardiomyocyte
differentiation
embryonic stem
human embryonic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0800264A
Other languages
English (en)
Other versions
GB2441718A (en
GB0800264D0 (en
Inventor
Joseph D Gold
Mohammad Hassanipour
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Geron Corp
Original Assignee
Geron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Geron Corp filed Critical Geron Corp
Publication of GB0800264D0 publication Critical patent/GB0800264D0/en
Publication of GB2441718A publication Critical patent/GB2441718A/en
Application granted granted Critical
Publication of GB2441718B publication Critical patent/GB2441718B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB0800264A 2005-06-22 2006-06-20 Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells Expired - Fee Related GB2441718B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69314105P 2005-06-22 2005-06-22
PCT/US2006/024060 WO2007002136A2 (en) 2005-06-22 2006-06-20 Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells

Publications (3)

Publication Number Publication Date
GB0800264D0 GB0800264D0 (en) 2008-02-13
GB2441718A GB2441718A (en) 2008-03-12
GB2441718B true GB2441718B (en) 2010-10-06

Family

ID=37595761

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0800264A Expired - Fee Related GB2441718B (en) 2005-06-22 2006-06-20 Differentiation of human embryonic stem cells to cardiomyocyte-lineage cells

Country Status (10)

Country Link
US (2) US9062289B2 (cg-RX-API-DMAC7.html)
EP (1) EP1910518B1 (cg-RX-API-DMAC7.html)
JP (2) JP5560393B2 (cg-RX-API-DMAC7.html)
KR (2) KR20130099253A (cg-RX-API-DMAC7.html)
CN (1) CN101228264B (cg-RX-API-DMAC7.html)
AU (1) AU2006262329B2 (cg-RX-API-DMAC7.html)
CA (1) CA2611809C (cg-RX-API-DMAC7.html)
GB (1) GB2441718B (cg-RX-API-DMAC7.html)
IL (1) IL187611A (cg-RX-API-DMAC7.html)
WO (1) WO2007002136A2 (cg-RX-API-DMAC7.html)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7666423B2 (en) * 2006-07-28 2010-02-23 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
US8106004B2 (en) * 2006-07-28 2012-01-31 Children's Memorial Hospital Methods of inhibiting tumor cell aggressiveness using the microenvironment of human embryonic stem cells
EP2273996B8 (en) 2008-03-27 2017-04-26 Icahn School of Medicine at Mount Sinai Human cardiovascular progenitor cells
EP4364797A3 (en) 2009-10-19 2024-07-10 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte production
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
JP6090855B2 (ja) 2010-11-17 2017-03-08 国立大学法人京都大学 心筋細胞および／または心筋前駆細胞増殖剤ならびに心筋細胞および／または心筋前駆細胞の増殖方法
EP2691512B1 (en) 2011-03-29 2019-05-01 Asterias Biotherapeutics, Inc. Enriched populations of cardiomyocyte lineage cells from pluripotent stem cells
JP6185907B2 (ja) 2011-03-30 2017-08-23 セルラー ダイナミクス インターナショナル， インコーポレイテッド 神経分化のための多能性幹細胞の予備刺激
JP5840855B2 (ja) 2011-03-30 2016-01-06 学校法人東京女子医科大学 胚性幹細胞から心筋シートを製造する方法
EP2691513A4 (en) 2011-03-31 2014-09-03 Iheart Japan Corp NEW CARDIOMYOCYTE MARKER
AU2012268950B2 (en) 2011-06-16 2016-02-04 Dolby Video Compression, Llc Entropy coding of motion vector differences
US9395354B2 (en) 2011-07-21 2016-07-19 The Board Of Trustees Of The Leland Stanford Junior University Cardiomyocytes from induced pluripotent stem cells from patients and methods of use thereof
GB201121101D0 (en) * 2011-12-08 2012-01-18 Nhs Blood & Transplant A method of preparing isolated cells and uses
EP2826855B1 (en) 2012-03-15 2018-08-29 iHeart Japan Corporation Myocardial sheet
JP6373253B2 (ja) 2012-07-17 2018-08-15 国立大学法人京都大学 新規心筋細胞マーカー
US9822342B2 (en) 2013-05-14 2017-11-21 Kyoto University Method of efficiently inducing cardiomyocytes
CN105247041B (zh) 2013-05-31 2018-04-20 爱心细胞有限公司 组入有水凝胶的层叠细胞膜片
WO2014192312A1 (ja) * 2013-05-31 2014-12-04 国立大学法人 東京医科歯科大学 正常な内向きのカリウム電流特性を有するｉＰＳ細胞由来心筋モデル細胞
DE112014002780B4 (de) 2013-06-11 2025-05-22 President And Fellows Of Harvard College SC-ß-Zellen und Zusammensetzungen und Verfahren zur Erzeugung der Zellen
EP3121276B1 (en) 2014-03-20 2019-04-17 Kyoto University Cardiomyocyte sorting method
KR20160147800A (ko) * 2014-05-06 2016-12-23 에프. 호프만-라 로슈 아게 다능성 줄기세포의 심근세포로의 분화 방법
WO2016043168A1 (ja) * 2014-09-16 2016-03-24 国立大学法人大阪大学 多能性幹細胞由来心筋細胞集団の製造方法
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2016100909A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED β CELLS AND USES THEREOF
US10556388B2 (en) * 2015-04-22 2020-02-11 Eastman Chemical Company Polyester-based tape composites for wood reinforcement
US10968426B2 (en) 2015-05-08 2021-04-06 President And Fellows Of Harvard College Universal donor stem cells and related methods
AU2018370029B2 (en) 2017-11-15 2024-11-07 Vertex Pharmaceuticals Incorporated Islet cell manufacturing compositions and methods of use
CN111918961B (zh) 2018-03-30 2023-10-24 国立大学法人京都大学 心肌细胞成熟促进剂
JP7440868B2 (ja) 2018-03-30 2024-02-29 国立大学法人京都大学 細胞の製造方法
EP3778587B1 (en) 2018-03-30 2023-10-04 Orizuru Therapeutics, Inc. Heterocyclic compound
JP7436990B2 (ja) 2018-08-10 2024-02-22 国立大学法人京都大学 カチオン性脂質を用いた心筋細胞へのトランスフェクション方法
DK3835287T3 (da) 2018-08-10 2025-01-02 Takeda Pharmaceuticals Co Kationisk lipid
AU2019320072A1 (en) 2018-08-10 2021-02-25 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
EP4017508A1 (en) 2019-08-23 2022-06-29 Sana Biotechnology, Inc. Cd24 expressing cells and uses thereof
KR20230074718A (ko) 2020-08-13 2023-05-31 사나 바이오테크놀로지, 인크. 저면역원성 세포로 감작된 환자를 치료하는 방법 및 관련 방법 및 조성물
US20240226164A1 (en) 2021-05-27 2024-07-11 Sana Biotechnology, Inc. Hypoimmunogenic cells comprising engineered hla-e or hla-g
KR20240046319A (ko) 2021-07-14 2024-04-08 사나 바이오테크놀로지, 인크. 저면역원성 세포에서의 y 염색체-연결 항원의 변경된 발현
TW202321435A (zh) 2021-08-11 2023-06-01 美商薩那生物科技公司 用於改變低免疫原性細胞中之基因表現之可誘導系統
US20240425820A1 (en) 2021-08-11 2024-12-26 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy to reduce complement-mediated inflammatory reactions
JP2024535677A (ja) 2021-08-11 2024-10-02 サナ バイオテクノロジー，インコーポレイテッド 即時血液媒介性炎症反応を減少させるための同種細胞療法を目的とした遺伝子改変細胞
AU2022326565A1 (en) 2021-08-11 2024-02-08 Sana Biotechnology, Inc. Genetically modified cells for allogeneic cell therapy
KR20230073749A (ko) 2021-11-19 2023-05-26 주식회사 로킷헬스케어 박동형 심근 패치의 제조방법 및 이를 이용하여 제조된 박동형 심근 패치
WO2023183313A1 (en) 2022-03-22 2023-09-28 Sana Biotechnology, Inc. Engineering cells with a transgene in b2m or ciita locus and associated compositions and methods
WO2024243236A2 (en) 2023-05-22 2024-11-28 Sana Biotechnology, Inc. Methods of delivery of islet cells and related methods
WO2025054202A1 (en) 2023-09-05 2025-03-13 Sana Biotechnology, Inc. Method of screening a sample comprising a transgene with a unique barcode
WO2025151838A1 (en) 2024-01-12 2025-07-17 Sana Biotechnology, Inc. Safety switches to control in vitro and in vivo proliferation of cell therapy products

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061837A1 (en) * 1996-09-05 2002-05-23 Lough John W. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
US20050054092A1 (en) * 2001-07-12 2005-03-10 Chunhui Xu Process for making transplantable cardiomyocytes from human embryonic stem cells

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4491632A (en) 1979-10-22 1985-01-01 The Massachusetts General Hospital Process for producing antibodies to hepatitis virus and cell lines therefor
US4444887A (en) 1979-12-10 1984-04-24 Sloan-Kettering Institute Process for making human antibody producing B-lymphocytes
EP0043718B1 (en) 1980-07-07 1984-11-28 National Research Development Corporation Improvements in or relating to cell lines
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
WO1992013066A1 (en) 1991-01-24 1992-08-06 Loyola University Of Chicago Mammalian cardiac myocyte cell line
US5543318A (en) * 1991-06-12 1996-08-06 Smith; David A. Method of isolation, culture and proliferation of human atrial myocytes
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US5602301A (en) 1993-11-16 1997-02-11 Indiana University Foundation Non-human mammal having a graft and methods of delivering protein to myocardial tissue
US6015671A (en) 1995-06-07 2000-01-18 Indiana University Foundation Myocardial grafts and cellular compositions
DE4441327C1 (de) 1994-11-22 1995-11-09 Inst Pflanzengenetik & Kultur Embryonale Herzmuskelzellen, ihre Herstellung und ihre Verwendung
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU725084B2 (en) 1996-08-16 2000-10-05 Mcw Research Foundation, Inc. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
US6261836B1 (en) 1996-10-01 2001-07-17 Geron Corporation Telomerase
WO1998030679A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6110459A (en) 1997-05-28 2000-08-29 Mickle; Donald A. G. Transplants for myocardial scars and methods and cellular preparations
US6099832A (en) 1997-05-28 2000-08-08 Genzyme Corporation Transplants for myocardial scars
ATE307195T1 (de) 1997-07-14 2005-11-15 Osiris Therapeutics Inc Herzmuskelregenerierung unter verwendung mesenchymaler stammzellen
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
DE19755800A1 (de) * 1997-12-16 1999-06-17 Merck Patent Gmbh Cyclopeptidderivate
JP2002507407A (ja) 1998-03-23 2002-03-12 ザイモジェネティクス，インコーポレイティド 心臓由来幹細胞
IL138502A0 (en) 1998-04-09 2001-10-31 Bresagen Ltd Cell differentiation/ proliferation and maintenaince factor and uses thereof
ATE382681T1 (de) 1998-07-31 2008-01-15 Genzyme Corp Verbesserung der herzfunktion durch transplantation mesenchymaler stammzellen
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US20020168766A1 (en) 2000-01-11 2002-11-14 Gold Joseph D. Genetically altered human pluripotent stem cells
IL129966A (en) 1999-05-14 2009-12-24 Technion Res & Dev Foundation ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS
CA2377270A1 (en) 1999-06-18 2000-12-28 Advanced Research And Technology Institute, Inc. Cardiomyocytes with enhanced proliferative potential
EP1221956A2 (en) 1999-09-30 2002-07-17 McGILL UNIVERSITY Autologous marrow stem cell (msc) transplantation for myocardial regeneration
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
AU784618B2 (en) 1999-12-28 2006-05-18 Kyowa Hakko Kogyo Co. Ltd. Cells capable of differentiating into heart muscle cells
US20020142457A1 (en) * 1999-12-28 2002-10-03 Akihiro Umezawa Cell having the potentiality of differentiation into cardiomyocytes
EP1254211A4 (en) 2000-01-14 2003-07-16 Bresagen Ltd PRODUCTION OF CELLS
WO2001053465A1 (en) 2000-01-21 2001-07-26 The Johns Hopkins University School Of Medicine Human embryoid body-derived cells
IL151605A0 (en) 2000-03-10 2003-04-10 Advanced Res & Tech Inst Cardiomyocyte cell populations, methods for obtaining the same and devices utilizing the same
US20020061587A1 (en) 2000-07-31 2002-05-23 Piero Anversa Methods and compositions for the repair and/or regeneration of damaged myocardium
EP1315510A4 (en) 2000-07-31 2005-08-03 New York Medical College METHODS AND COMPOSITIONS FOR THE REPAIR AND / OR REGENERATION OF DAMAGED MYOCARDE
AU2001286173B2 (en) * 2000-08-01 2007-10-25 Yissum Research Development Company Directed differentiation of embryonic cells
US6607720B1 (en) 2000-09-05 2003-08-19 Yong-Fu Xiao Genetically altered mammalian embryonic stem cells, their living progeny, and their therapeutic application for improving cardiac function after myocardial infarction
US6534052B1 (en) 2000-09-05 2003-03-18 Yong-Fu Xiao Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated
EP1335972A2 (en) 2000-11-22 2003-08-20 Geron Corporation Tolerizing allografts of pluripotent stem cells
WO2002083864A2 (en) 2001-04-13 2002-10-24 Anterogen Co., Ltd. Methods and reagents for cell transplantation
KR101073411B1 (ko) * 2001-07-12 2011-10-17 제론 코포레이션 인간 다분화능 줄기 세포로부터 제조된 심근 세포 계통의 세포
WO2003008535A2 (en) 2001-07-20 2003-01-30 Technion Research And Development Foundation Ltd. Methods of generating human cardiac cells and tissues and uses thereof
AU2002317039B2 (en) 2001-07-24 2007-10-04 Es Cell International Pte Ltd Methods of inducing differentiation of stem cells
IL162130A0 (en) 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
US8298606B2 (en) * 2002-03-08 2012-10-30 The Regents Of The University Of California Methods and compositions for stabilizing the myocardium
CN100523213C (zh) * 2002-03-15 2009-08-05 威塞尔研究所股份有限公司 产生灵长类动物滋养层的方法
US7247477B2 (en) 2002-04-16 2007-07-24 Technion Research & Development Foundation Ltd. Methods for the in-vitro identification, isolation and differentiation of vasculogenic progenitor cells
US6898272B2 (en) * 2002-08-01 2005-05-24 Spirent Communications System and method for testing telecommunication devices
US7569222B2 (en) * 2002-11-18 2009-08-04 Woerly Stephane Hydrogel membrane composition and use thereof
US20050031598A1 (en) 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
AU2003901099A0 (en) 2003-03-11 2003-03-27 Es Cell International Pte Ltd. Methods of inducing differentiation of stem cells
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US20050266554A1 (en) * 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
US20050142121A1 (en) 2003-12-24 2005-06-30 Gold Joseph D. System for improving acceptance of tissue grafts made from embryonic stem cells
CA2559854C (en) * 2004-03-19 2014-12-02 Geron Corporation Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
US20050214938A1 (en) * 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
US7452718B2 (en) * 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US8932620B2 (en) * 2005-06-17 2015-01-13 Drexel University Three-dimensional scaffolds for tissue engineering made by processing complex extracts of natural extracellular matrices
JP2010530955A (ja) * 2006-12-29 2010-09-16 ユニヴァーシティ オブ ワシントン 二重機能性非汚染表面および材料
CA2713754A1 (en) * 2008-01-30 2009-08-13 Geron Corporation Synthetic surfaces for culturing cells in chemically defined media
JP5792714B2 (ja) * 2009-05-28 2015-10-14 コーニング インコーポレイテッド 細胞培養のための膨潤性の合成マイクロキャリア

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020061837A1 (en) * 1996-09-05 2002-05-23 Lough John W. Bone morphogenetic protein and fibroblast growth factor compositions and methods for the induction of cardiogenesis
US20050054092A1 (en) * 2001-07-12 2005-03-10 Chunhui Xu Process for making transplantable cardiomyocytes from human embryonic stem cells

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