FR3062394B1 - Microorganisme genetiquement optimise pour la production de molecules d'interet - Google Patents

Microorganisme genetiquement optimise pour la production de molecules d'interet Download PDF

Info

Publication number
FR3062394B1
FR3062394B1 FR1750694A FR1750694A FR3062394B1 FR 3062394 B1 FR3062394 B1 FR 3062394B1 FR 1750694 A FR1750694 A FR 1750694A FR 1750694 A FR1750694 A FR 1750694A FR 3062394 B1 FR3062394 B1 FR 3062394B1
Authority
FR
France
Prior art keywords
interest
molecules
production
microorganism
genetically modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1750694A
Other languages
English (en)
French (fr)
Other versions
FR3062394A1 (fr
Inventor
Cedric Boisart
Nicolas Morin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EPPEN EUROPE, FR
Original Assignee
Enobraq
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1750694A priority Critical patent/FR3062394B1/fr
Application filed by Enobraq filed Critical Enobraq
Priority to BR112019015458-2A priority patent/BR112019015458A2/pt
Priority to KR1020197024086A priority patent/KR102643557B1/ko
Priority to PCT/EP2018/052005 priority patent/WO2018138289A1/fr
Priority to JP2019561349A priority patent/JP7240329B2/ja
Priority to EP18702647.1A priority patent/EP3574083A1/fr
Priority to CN201880021659.1A priority patent/CN110678541A/zh
Priority to US16/480,569 priority patent/US20200277592A1/en
Publication of FR3062394A1 publication Critical patent/FR3062394A1/fr
Application granted granted Critical
Publication of FR3062394B1 publication Critical patent/FR3062394B1/fr
Priority to US17/573,877 priority patent/US12018300B2/en
Priority to JP2022182031A priority patent/JP2023011947A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01044—Phosphogluconate dehydrogenase (decarboxylating) (1.1.1.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01012—Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (1.2.1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02003—Phosphoglycerate kinase (2.7.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01031—6-Phosphogluconolactonase (3.1.1.31)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
FR1750694A 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet Active FR3062394B1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR1750694A FR3062394B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
US16/480,569 US20200277592A1 (en) 2017-01-27 2018-01-26 Genetically optimised microorganism for producing molecules of interest
PCT/EP2018/052005 WO2018138289A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
JP2019561349A JP7240329B2 (ja) 2017-01-27 2018-01-26 目的分子を産生するための遺伝的に最適化された微生物
EP18702647.1A EP3574083A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
CN201880021659.1A CN110678541A (zh) 2017-01-27 2018-01-26 用于产生感兴趣的分子的经遗传优化的微生物
BR112019015458-2A BR112019015458A2 (pt) 2017-01-27 2018-01-26 Microrganismo geneticamente otimizado para produzir moléculas de interesse
KR1020197024086A KR102643557B1 (ko) 2017-01-27 2018-01-26 대상 분자의 생성을 위한 유전자 최적화 미생물
US17/573,877 US12018300B2 (en) 2017-01-27 2022-01-12 Genetically optimised microorganism for producing molecules of interest
JP2022182031A JP2023011947A (ja) 2017-01-27 2022-11-14 目的分子を産生するための遺伝的に最適化された微生物

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1750694 2017-01-27
FR1750694A FR3062394B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet

Publications (2)

Publication Number Publication Date
FR3062394A1 FR3062394A1 (fr) 2018-08-03
FR3062394B1 true FR3062394B1 (fr) 2021-04-16

Family

ID=59070757

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1750694A Active FR3062394B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet

Country Status (8)

Country Link
US (2) US20200277592A1 (enExample)
EP (1) EP3574083A1 (enExample)
JP (2) JP7240329B2 (enExample)
KR (1) KR102643557B1 (enExample)
CN (1) CN110678541A (enExample)
BR (1) BR112019015458A2 (enExample)
FR (1) FR3062394B1 (enExample)
WO (1) WO2018138289A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2902149C (en) * 2013-02-22 2022-07-26 Technische Universiteit Delft Recombinant micro-organism for use in method with increased product yield
WO2017216136A1 (en) * 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
CA3136574A1 (en) * 2019-05-10 2020-11-19 Jung Joon Min Dna construct for diagnosing and treating cancer
JP6917434B2 (ja) * 2019-10-25 2021-08-11 長瀬産業株式会社 好気性菌の代謝酵素破壊株およびその培養法
CN112852664B (zh) * 2021-01-14 2022-07-15 西华大学 一种酿酒酵母以及提高酿酒酵母产γ-氨基丁酸的方法
CN112941061B (zh) * 2021-03-19 2021-09-07 中国农业大学 一种Tk-Rubisco突变体、线性组装材料及其制备方法和应用
CN114164237B (zh) * 2021-11-04 2023-03-17 山东阜丰发酵有限公司 一种生产琥珀酸的方法
CN114181879B (zh) * 2021-12-09 2022-11-22 中国科学院深圳先进技术研究院 具有自养能力的底盘细胞及其构建方法和应用
CN115851779B (zh) * 2022-10-29 2024-03-26 昆明理工大学 一种葡萄糖-6-磷酸脱氢酶基因RkZWF1及其应用
CN117210446A (zh) * 2023-03-21 2023-12-12 北京蓝晶微生物科技有限公司 一种重建卡尔文循环提高异养微生物代谢产物产量的方法
CN120384059B (zh) * 2025-06-27 2025-09-26 南京大学 葡萄糖-6-磷酸脱氢酶突变体、生物材料、催化剂及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9915191A (pt) 1998-11-10 2001-12-11 Maxygen Inc Métodos para obter um polinucleotìdeo isolado epara produzir uma célula recombinante,protoplasto de célula vegetal, coleção deprotoplastos de célula vegetal, vegetal,polinucleotìdeo, biblioteca, um ou mais membros debiblioteca e célula recombinante alvo
US7826975B2 (en) 2002-07-10 2010-11-02 The Penn State Research Foundation Method for redesign of microbial production systems
WO2009036095A1 (en) 2007-09-10 2009-03-19 Joule Biotechnologies, Inc. Engineered light-harvesting organisms
FR3016371B1 (fr) * 2014-01-16 2018-02-02 Institut National De La Recherche Agronomique Levures modifiees pour utiliser le dioxyde de carbone
EP3145947B1 (en) * 2014-05-22 2025-02-12 Yeda Research and Development Co., Ltd. Recombinant microorganisms capable of carbon fixation

Also Published As

Publication number Publication date
EP3574083A1 (fr) 2019-12-04
JP7240329B2 (ja) 2023-03-15
WO2018138289A1 (fr) 2018-08-02
US12018300B2 (en) 2024-06-25
JP2023011947A (ja) 2023-01-24
US20200277592A1 (en) 2020-09-03
CN110678541A (zh) 2020-01-10
US20220348897A1 (en) 2022-11-03
FR3062394A1 (fr) 2018-08-03
JP2020506723A (ja) 2020-03-05
KR20190115447A (ko) 2019-10-11
KR102643557B1 (ko) 2024-03-07
BR112019015458A2 (pt) 2020-03-31

Similar Documents

Publication Publication Date Title
FR3062395B1 (fr) Microorganisme genetiquement optimise pour la production de molecules d'interet
Lloyd et al. Characterization of tRNA-dependent peptide bond formation by MurM in the synthesis of Streptococcus pneumoniae peptidoglycan
Otsuka et al. Application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation
DK2049673T3 (da) Native kornamylaser i enzymkombinationer til hydrolyse af granulær stivelse
BR0009362A (pt) Variante de uma alfa-amilase semelhante atermamyl precursora, construção de dna, vetorde expressão recombinante, célula, composição,processo para gerar uma variante de umaalfa-amilase semelhante a termamyl precursora,e, uso de uma variante de alfa-amilase
WO2010101628A3 (en) Methods and products for in vivo enzyme profiling
RU2012128537A (ru) Композиция из гидролизованного цельного зерна
Mine et al. Enhanced enzyme activity and enantioselectivity of lipases in organic solvents by crown ethers and cyclodextrins
WO2008088493A3 (en) Compositions and uses for an alpha-amylase polypeptide of bacillus species 195
MX344970B (es) Medio de cultivo celular para la expresion de la proteina adamts.
BR112021017128A2 (pt) Composições conservantes e métodos de uso das mesmas
WO2018226170A3 (en) MUTANT STRAIN OF CLOSTRIDIUM THERMOCELLUM FOR THE PRODUCTION OF CELLULASE AND XYLANASE AND PREPARATION METHOD THEREOF
Haddad Momeni et al. Loss of AA13 LPMOs impairs degradation of resistant starch and reduces the growth of Aspergillus nidulans
Abdullah et al. Reversible action of pullulanase
NO20083035L (no) Enzymer for redusert immunologisk stress
Pozelli Macedo et al. Biochemical properties of a Flavobacterium johnsoniae dextranase and its biotechnological potential for Streptococcus mutans biofilm degradation
Pérez et al. Aspergillus fumigatus produces two arabinofuranosidases from glycosyl hydrolase family 62: comparative properties of the recombinant enzymes
WO2023122689A3 (en) Complement factor i-related compositions and methods
Spinelli et al. Biochemical characterization of glucoamylase from the hyperproducer exo-1 mutant strain of Neurospora crassa
Boucherba et al. Purification and characterization of the xylanase produced by Jonesia denitrificans BN-13
Hemamalini et al. Halophilic lipase does forms catalytically active aggregates: Evidence from Marinobacter sp. EMB5 lipase (LipEMB5)
Mortensen et al. Recognition of C-terminal amide groups by (serine) carboxypeptidase Y investigated by site-directed mutagenesis.
Shehata et al. Extraction, purification and characterization of endo-acting pullulanase type I from white edible mushrooms
Leo et al. Characterization of MdpS: an in-depth analysis of a MUC5B-degrading protease from Streptococcus oralis
Kröger et al. Influence of cortisone on the substrate-induction of enzymes in rat liver

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20180803

PLFP Fee payment

Year of fee payment: 4

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6

TP Transmission of property

Owner name: EPPEN EUROPE, FR

Effective date: 20220801

PLFP Fee payment

Year of fee payment: 7

PLFP Fee payment

Year of fee payment: 8

PLFP Fee payment

Year of fee payment: 9

PLFP Fee payment

Year of fee payment: 10