FI120353B - Transgena Arabidopsis thaliana-växter som producerar polyhydroxialkanoat i form av granuler - Google Patents

Transgena Arabidopsis thaliana-växter som producerar polyhydroxialkanoat i form av granuler Download PDF

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Publication number
FI120353B
FI120353B FI940242A FI940242A FI120353B FI 120353 B FI120353 B FI 120353B FI 940242 A FI940242 A FI 940242A FI 940242 A FI940242 A FI 940242A FI 120353 B FI120353 B FI 120353B
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phb
gene
plants
plant
transgenic
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FI940242A
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FI940242A0 (sv
FI940242A (sv
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Christopher Roland Somerville
Yves Poirier
Douglas Edward Dennis
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Univ Michigan State
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Claims (9)

1. Transgent Arabidops is thaliana-växtmaterial, soin imieMllar främmande DNA, som härstammar frän en orga- 5 nism som förmAr produeera polyhydroxialkahoat och som kp-dar för en 3-ketotiolasgen, en acetoacetyl“CQA--reduktasgen och en PHB-syntasgen, vilket främmande DNA leder till produktion av pölyhydroxialkanoat, varvid polyhydroxialkanoa-tet ar f öreträdesvis: polyhydroxismörsyra och vilket poly-10 hydroxialkanoat Sr i form av granular.
2. VSxtmaterial enligt patentkrav 2, väri främmande DNAiS kodande sekvens Sr sSsom visats i SEQ ID N0:1.
3. VSxtmaterial enligt patentkrav 1 ellet 2, som Sr ett frö eller fröets förökningsorgan, 15 4.: Förfarande: för att föra in främmande DNA i en växt, värvid nämnda DNA kodar för polypeptider som leder tili en syntes av pölyhydroxialkanoat i form av granuler, vilket förfarande omfattar steg, i vilka erbjuds för an-vändning tvä växter och förs in i vardera växtens DNA-20 sekvens främmande DNA, som kodar för ett eller flera olika enzym av en rutt som leder tili polymerisering av hydroxi-alkyl-CoA som ästadkommits med polyhydroxialkanoatsyntas, för att ästadkomma tvä växter som innehäller främmande DNA, vilka inte bildar pölyhydroxialkanoat, och dessa tvä 25 växter som innehäller främmande DNA, vilka inte bildar po-lyhydroxialkanoat, paras genom könlig befruktning för att ästadkomma en växt som kodar för pölyhydroxialkanoat, varvid polyhydroxialkanoatet är företrädesvis polyhydroxis-mörsyra. 30 5. Växtmaterial, som innehäller ett gensegment, väri segmentet innehäller DNA som kodar för en gen av 3— ketotiolas och segmentet ingär i ett frö, som deponerats med ÄTGC-nummer 75042.
6. Växtmaterial, som innehäller ett gensegment, 35 väri segmentet. innehäller DNA som kodar för en gen av ace-toaeetyl-CoA-reduktas och segmentet ingär i ett frö, som deponerats med ATCC-nummer 75044. 43
7. Växtmaterial, sorti inneMller ett genseginent, väri segment et innelialler DNA sotu kodar för en gen av PHB-syntas och segment et ingär i ett frö, som deponerats med ATCC-nuitimer 75 043 . 5 8. Växtmaterial enligt nägot av patentkraven 5-7, varvi d genseginentet är underkastat transkriptionell re-glering av en DNA-sekvens som inducerar transkriptioner i växtceller.
9. Växtmaterial enligt patentkrav 8, varvid gen-10 segmentet är underkastat transkriptionell regiering av en CaMV 35S-promotor.
FI940242A 1991-07-19 1994-01-18 Transgena Arabidopsis thaliana-växter som producerar polyhydroxialkanoat i form av granuler FI120353B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US73224391A 1991-07-19 1991-07-19
US73224391 1991-07-19
PCT/US1992/005786 WO1993002187A1 (en) 1991-07-19 1992-07-13 Transgenic plants producing polyhydroxyalkanoates
US9205786 1992-07-13

Publications (3)

Publication Number Publication Date
FI940242A0 FI940242A0 (sv) 1994-01-18
FI940242A FI940242A (sv) 1994-03-11
FI120353B true FI120353B (sv) 2009-09-30

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FI940242A FI120353B (sv) 1991-07-19 1994-01-18 Transgena Arabidopsis thaliana-växter som producerar polyhydroxialkanoat i form av granuler

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US (1) US5650555A (sv)
EP (1) EP0595896B1 (sv)
JP (1) JP3696233B2 (sv)
AT (1) ATE295891T1 (sv)
AU (1) AU671953B2 (sv)
BE (1) BE1006851A5 (sv)
BR (1) BR9206297A (sv)
CA (1) CA2113412C (sv)
CZ (1) CZ10894A3 (sv)
DE (1) DE69233512T2 (sv)
ES (1) ES2046142B1 (sv)
FI (1) FI120353B (sv)
FR (1) FR2679735A1 (sv)
HU (1) HUT66825A (sv)
IT (1) IT1255348B (sv)
MX (1) MX9204245A (sv)
NL (1) NL195045C (sv)
NZ (1) NZ243626A (sv)
PL (1) PL170467B1 (sv)
PT (1) PT100705B (sv)
SK (1) SK6494A3 (sv)
WO (1) WO1993002187A1 (sv)

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ES2046142A1 (es) 1994-01-16
FR2679735A1 (fr) 1993-02-05
JP3696233B2 (ja) 2005-09-14
FI940242A0 (sv) 1994-01-18
IT1255348B (it) 1995-10-31
DE69233512T2 (de) 2005-10-13
MX9204245A (es) 1993-02-01
DE69233512D1 (de) 2005-06-23
NZ243626A (en) 1995-03-28
EP0595896B1 (en) 2005-05-18
EP0595896A1 (en) 1994-05-11
ITMI921750A1 (it) 1994-01-17
FR2679735B1 (sv) 1995-05-05
AU2323892A (en) 1993-02-23
CZ10894A3 (en) 1994-12-15
HUT66825A (en) 1995-01-30
SK6494A3 (en) 1994-10-05
ATE295891T1 (de) 2005-06-15
BE1006851A5 (fr) 1995-01-10
WO1993002187A1 (en) 1993-02-04
NL9201288A (nl) 1993-02-16
PT100705A (pt) 1993-10-29
AU671953B2 (en) 1996-09-19
ITMI921750A0 (it) 1992-07-17
PT100705B (pt) 1999-06-30
PL170467B1 (pl) 1996-12-31
ES2046142B1 (es) 1994-10-01
JPH06509233A (ja) 1994-10-20
CA2113412C (en) 2000-01-25
US5650555A (en) 1997-07-22
HU9400145D0 (en) 1994-05-30
PL295350A1 (en) 1993-04-05
NL195045C (nl) 2003-06-27
CA2113412A1 (en) 1993-02-04
EP0595896A4 (en) 1994-08-24
FI940242A (sv) 1994-03-11
BR9206297A (pt) 1995-10-17

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