FI110693B - Menetelmä Aspergillus-kantojen rakentamiseksi geenikorvauksen avulla - Google Patents

Menetelmä Aspergillus-kantojen rakentamiseksi geenikorvauksen avulla Download PDF

Info

Publication number
FI110693B
FI110693B FI893818A FI893818A FI110693B FI 110693 B FI110693 B FI 110693B FI 893818 A FI893818 A FI 893818A FI 893818 A FI893818 A FI 893818A FI 110693 B FI110693 B FI 110693B
Authority
FI
Finland
Prior art keywords
gene
chymosin
expression
filamentous fungal
fungal host
Prior art date
Application number
FI893818A
Other languages
English (en)
Finnish (fi)
Swedish (sv)
Other versions
FI893818A0 (fi
FI893818A (fi
Inventor
Hartingsveldt Wim Van
Den Hondel Cees A M J J Van
Annemarie E Veenstra
Den Berg Johannes A Van
Original Assignee
Dsm Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8199839&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI110693(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Nv filed Critical Dsm Nv
Publication of FI893818A0 publication Critical patent/FI893818A0/fi
Publication of FI893818A publication Critical patent/FI893818A/fi
Application granted granted Critical
Publication of FI110693B publication Critical patent/FI110693B/fi

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
FI893818A 1988-08-16 1989-08-14 Menetelmä Aspergillus-kantojen rakentamiseksi geenikorvauksen avulla FI110693B (fi)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP88201743 1988-08-16
EP88201743 1988-08-16

Publications (3)

Publication Number Publication Date
FI893818A0 FI893818A0 (fi) 1989-08-14
FI893818A FI893818A (fi) 1990-02-17
FI110693B true FI110693B (fi) 2003-03-14

Family

ID=8199839

Family Applications (1)

Application Number Title Priority Date Filing Date
FI893818A FI110693B (fi) 1988-08-16 1989-08-14 Menetelmä Aspergillus-kantojen rakentamiseksi geenikorvauksen avulla

Country Status (14)

Country Link
EP (1) EP0357127B1 (de)
JP (1) JP2752714B2 (de)
KR (1) KR900003366A (de)
AT (1) ATE181111T1 (de)
AU (1) AU636572B2 (de)
CA (1) CA1341226C (de)
DE (1) DE68929013T2 (de)
DK (1) DK175196B1 (de)
ES (1) ES2134754T3 (de)
FI (1) FI110693B (de)
GR (1) GR3030901T3 (de)
HU (1) HUT51336A (de)
IL (1) IL91309A (de)
PT (1) PT91453B (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2034487C (en) * 1989-06-16 2000-08-29 Virgil B. Lawlis Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
NL9001388A (nl) 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
IE20040144A1 (en) * 1990-07-24 2004-05-19 Dsm Nv Cloning and expression of xylanase genes from fungal origin
DE69231995T2 (de) * 1991-12-09 2002-04-04 Unilever Plc Verfahren zur Herstellung/Sekretion eines Proteins durch einen transformiertenSchimmelpilz unter Verwendung von Expressions-/Sekretions- Regulationsregionenaus Aspergillus
AU4383293A (en) * 1992-05-19 1993-12-13 Tsi Corporation Production of transgenics by joining regulatory and coding regions in vivo
WO1995017513A1 (en) * 1993-12-23 1995-06-29 Novo Nordisk A/S Retransformation of filamentous fungi
BE1009488A4 (fr) * 1994-06-17 1997-04-01 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
BE1008267A3 (fr) * 1994-01-28 1996-03-05 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
BE1008737A3 (fr) * 1994-01-28 1996-07-02 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
WO1998046772A2 (en) * 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
EP2172557B1 (de) 2004-04-02 2018-03-14 DSM IP Assets B.V. Fadenpilzmutanten mit verbesserter homologer Rekombinationseffizient
EP2345726A3 (de) 2004-04-16 2011-12-14 DSM IP Assets B.V. Promotoren aus Fungi für die Expression eines Gens in einer Zelle aus Fungus
EP1799821A2 (de) 2004-10-15 2007-06-27 DSMIP Assets B.V. Homologe amds-gene als selektierbare marker
PL2410048T3 (pl) 2005-01-24 2017-02-28 Dsm Ip Assets B.V. Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego
AU2006316397B2 (en) 2005-11-28 2013-03-28 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
CA2630455A1 (en) 2005-11-29 2007-06-07 Dsm Ip Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
BRPI0619091A2 (pt) 2005-12-01 2011-09-13 Dsm Ip Assests Bv novos genes úteis para a produção industrial de ácido cìtrico
US8389269B2 (en) 2006-11-02 2013-03-05 Dsm Ip Assets B.V. Production of secreted proteins by filamentous fungi
WO2008098933A1 (en) 2007-02-15 2008-08-21 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
WO2008138835A1 (en) 2007-05-09 2008-11-20 Novozymes A/S Expression cloning method suitable for selecting library clones producing a polypeptide of interest
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
EA201300074A1 (ru) 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
BR112014031526A2 (pt) 2012-06-19 2017-08-01 Dsm Ip Assets Bv promotores para expressar um gene em uma célula
WO2014003555A1 (en) 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production
DK2875119T3 (en) 2012-07-19 2018-04-03 Dsm Ip Assets Bv AGSE-DEFICIENT TRIALS
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
MX370305B (es) 2013-12-02 2019-12-09 Dsm Ip Assets Bv Proteína estructurante de hielo.
MX2017005981A (es) 2014-11-07 2017-06-29 Dupont Nutrition Biosci Aps Celula hospedadora recombinante que expresa actividad de beta-galactosidasa y/o transgalactosidante deficiente en mananasa, celulasa y pectinasa.
EP3242948B1 (de) 2015-01-06 2021-12-22 DSM IP Assets B.V. Crispr-cas-system für eine yarrowia wirtszelle
DK3242949T3 (da) 2015-01-06 2022-01-24 Dsm Ip Assets Bv Crispr-cas-system til en gærværtscelle
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
NZ737305A (en) 2015-06-02 2021-07-30 Dsm Ip Assets Bv Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP3596199A1 (de) 2017-03-13 2020-01-22 DSM IP Assets B.V. Transkriptioneller regulator-defizienz-stamm mit zweikernigem zink-cluster

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) * 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
US4693536A (en) * 1986-06-12 1987-09-15 Molex Incorporated Insulation displacement terminal
EP0383779B2 (de) * 1987-09-04 2000-05-31 Novo Nordisk A/S VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN $i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN $i(ASPERGILLUS)

Also Published As

Publication number Publication date
CA1341226C (en) 2001-05-01
PT91453A (pt) 1990-03-08
JP2752714B2 (ja) 1998-05-18
EP0357127B1 (de) 1999-06-09
AU4002189A (en) 1990-02-22
HUT51336A (en) 1990-04-28
GR3030901T3 (en) 1999-11-30
IL91309A0 (en) 1990-03-19
IL91309A (en) 1997-11-20
FI893818A0 (fi) 1989-08-14
AU636572B2 (en) 1993-05-06
PT91453B (pt) 1995-05-31
KR900003366A (ko) 1990-03-26
ATE181111T1 (de) 1999-06-15
DK399889A (da) 1990-02-17
DE68929013D1 (de) 1999-07-15
DK175196B1 (da) 2004-07-05
FI893818A (fi) 1990-02-17
EP0357127A1 (de) 1990-03-07
DK399889D0 (da) 1989-08-15
DE68929013T2 (de) 1999-10-21
JPH02174673A (ja) 1990-07-06
ES2134754T3 (es) 1999-10-16

Similar Documents

Publication Publication Date Title
FI110693B (fi) Menetelmä Aspergillus-kantojen rakentamiseksi geenikorvauksen avulla
US11827891B2 (en) Protease deficient filamentous fungal cells and methods of use thereof
FI81379B (fi) Anvaendning av kluyveromyces-jaest saosom vaerd foer transformering och uttryckning av fraemmande gener.
Moralejo et al. Thaumatin production in Aspergillus awamori by use of expression cassettes with strong fungal promoters and high gene dosage
JP4896013B2 (ja) 改良された分泌によるポリペプチドの製造
Verdoes et al. The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene
FI108147B (fi) Menetelmä proteiinituotteiden tuottamiseksi Aspergillus oryzaella ja promoottori käytettävksi Aspergilluksessa
JP3068139B2 (ja) 酵母よりヒト血清アルブミンおよびその他の異種蛋白質を微生物学的に生産する方法
JP2016523552A (ja) 多重プロテアーゼ欠損糸状菌細胞及びその使用方法
US20180215797A1 (en) Regulatory Protein Deficient Trichoderma Cells and Methods of Use Thereof
CN104508114A (zh) 用于在细胞中表达基因的启动子
JPH04500313A (ja) 糸状菌からの目的とするポリペプチドの分泌を増幅するdna配列、ベクター、及び融合ポリペプチド
Klabunde et al. Increase of calnexin gene dosage boosts the secretion of heterologous proteins by Hansenula polymorpha
CN113015782A (zh) 用于酵母的前导序列
Michaelis et al. Identification of CBS2 as a mitochondrial protein in Saccharomyces cerevisiae
JPH08501695A (ja) 組換え真核細胞による分泌タンパク質の増大された産生
Heintel et al. Expression, processing and high level secretion of a virus toxin in fission yeast
AU734541B2 (en) Increased production of secreted proteins by recombinant yeast cells
JPH06503718A (ja) メチロトローフ酵母細胞におけるヒトリゾチムの産生とその効率的な分泌
SU1364241A3 (ru) Способ конструировани рекомбинантных векторов УСR р 2,УСR р 3 и УСR р 4
JPH03262487A (ja) ヒト血清アルブミンの安定な製造方法
TW394794B (en) D-amino acid oxidase promoter sequence from T.variabilis
Da Silva Expression and secretion of foreign proteins by'Aspergillus'
EP0245529A1 (de) Rekombinanter DNA-Vektor, der eine lytische bakterielle Transglykosylase kodiert und deren Anwendung zur Herstellung von 1,6-Anhydromuramylpeptiden
CS260920B1 (en) Recombination plasmid-coded polypeptide giving active calf's chymosin and method of its production

Legal Events

Date Code Title Description
GB Transfer or assigment of application

Owner name: DSM N.V.

MD Opposition filed

Owner name: DSM IP ASSETS B.V.

FG Patent granted

Ref document number: 110693

Country of ref document: FI

Effective date: 20030314

RF Appeal filed
FCK Appeal rejected

Free format text: PATENT IN FORCE