DK175196B1 - Transformeret filamentös fungusvært med en ekspressionskassette, DNA-konstruktion med en sådan ekspressionskassette og fremgangsmåde til fremstilling af et protein under anvendelse af en sådan transformert filamentös fungusvært - Google Patents

Transformeret filamentös fungusvært med en ekspressionskassette, DNA-konstruktion med en sådan ekspressionskassette og fremgangsmåde til fremstilling af et protein under anvendelse af en sådan transformert filamentös fungusvært Download PDF

Info

Publication number
DK175196B1
DK175196B1 DK198903998A DK399889A DK175196B1 DK 175196 B1 DK175196 B1 DK 175196B1 DK 198903998 A DK198903998 A DK 198903998A DK 399889 A DK399889 A DK 399889A DK 175196 B1 DK175196 B1 DK 175196B1
Authority
DK
Denmark
Prior art keywords
gene
filamentous fungus
interest
transformed
expression
Prior art date
Application number
DK198903998A
Other languages
Danish (da)
English (en)
Other versions
DK399889A (da
DK399889D0 (da
Inventor
Annemarie E Veenstra
Johannes A Van Den Berg
Wim Van Hartingsveldt
Cees A M J J Van Den Hondel
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8199839&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK175196(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of DK399889D0 publication Critical patent/DK399889D0/da
Publication of DK399889A publication Critical patent/DK399889A/da
Application granted granted Critical
Publication of DK175196B1 publication Critical patent/DK175196B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
DK198903998A 1988-08-16 1989-08-15 Transformeret filamentös fungusvært med en ekspressionskassette, DNA-konstruktion med en sådan ekspressionskassette og fremgangsmåde til fremstilling af et protein under anvendelse af en sådan transformert filamentös fungusvært DK175196B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP88201743 1988-08-16
EP88201743 1988-08-16

Publications (3)

Publication Number Publication Date
DK399889D0 DK399889D0 (da) 1989-08-15
DK399889A DK399889A (da) 1990-02-17
DK175196B1 true DK175196B1 (da) 2004-07-05

Family

ID=8199839

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198903998A DK175196B1 (da) 1988-08-16 1989-08-15 Transformeret filamentös fungusvært med en ekspressionskassette, DNA-konstruktion med en sådan ekspressionskassette og fremgangsmåde til fremstilling af et protein under anvendelse af en sådan transformert filamentös fungusvært

Country Status (14)

Country Link
EP (1) EP0357127B1 (de)
JP (1) JP2752714B2 (de)
KR (1) KR900003366A (de)
AT (1) ATE181111T1 (de)
AU (1) AU636572B2 (de)
CA (1) CA1341226C (de)
DE (1) DE68929013T2 (de)
DK (1) DK175196B1 (de)
ES (1) ES2134754T3 (de)
FI (1) FI110693B (de)
GR (1) GR3030901T3 (de)
HU (1) HUT51336A (de)
IL (1) IL91309A (de)
PT (1) PT91453B (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2034487C (en) * 1989-06-16 2000-08-29 Virgil B. Lawlis Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
NL9001388A (nl) 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
IE20040144A1 (en) * 1990-07-24 2004-05-19 Dsm Nv Cloning and expression of xylanase genes from fungal origin
DE69231995T2 (de) * 1991-12-09 2002-04-04 Unilever Plc Verfahren zur Herstellung/Sekretion eines Proteins durch einen transformiertenSchimmelpilz unter Verwendung von Expressions-/Sekretions- Regulationsregionenaus Aspergillus
AU4383293A (en) * 1992-05-19 1993-12-13 Tsi Corporation Production of transgenics by joining regulatory and coding regions in vivo
WO1995017513A1 (en) * 1993-12-23 1995-06-29 Novo Nordisk A/S Retransformation of filamentous fungi
BE1009488A4 (fr) * 1994-06-17 1997-04-01 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
BE1008267A3 (fr) * 1994-01-28 1996-03-05 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
BE1008737A3 (fr) * 1994-01-28 1996-07-02 Solvay Systeme d'expression, vecteur d'integration et cellule transformee par ce vecteur d'integration.
WO1998046772A2 (en) * 1997-04-11 1998-10-22 Dsm N.V. Gene conversion as a tool for the construction of recombinant industrial filamentous fungi
TW409035B (en) 1997-06-04 2000-10-21 Gist Brocades Bv Starch-based enzyme granulates
EP2172557B1 (de) 2004-04-02 2018-03-14 DSM IP Assets B.V. Fadenpilzmutanten mit verbesserter homologer Rekombinationseffizient
EP2345726A3 (de) 2004-04-16 2011-12-14 DSM IP Assets B.V. Promotoren aus Fungi für die Expression eines Gens in einer Zelle aus Fungus
EP1799821A2 (de) 2004-10-15 2007-06-27 DSMIP Assets B.V. Homologe amds-gene als selektierbare marker
PL2410048T3 (pl) 2005-01-24 2017-02-28 Dsm Ip Assets B.V. Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego
AU2006316397B2 (en) 2005-11-28 2013-03-28 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
CA2630455A1 (en) 2005-11-29 2007-06-07 Dsm Ip Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
BRPI0619091A2 (pt) 2005-12-01 2011-09-13 Dsm Ip Assests Bv novos genes úteis para a produção industrial de ácido cìtrico
US8389269B2 (en) 2006-11-02 2013-03-05 Dsm Ip Assets B.V. Production of secreted proteins by filamentous fungi
WO2008098933A1 (en) 2007-02-15 2008-08-21 Dsm Ip Assets B.V. A recombinant host cell for the production of a compound of interest
WO2008138835A1 (en) 2007-05-09 2008-11-20 Novozymes A/S Expression cloning method suitable for selecting library clones producing a polypeptide of interest
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
EA201300074A1 (ru) 2010-07-01 2013-06-28 ДСМ АйПи АССЕТС Б.В. Способ получения представляющего интерес соединения
BR112014031526A2 (pt) 2012-06-19 2017-08-01 Dsm Ip Assets Bv promotores para expressar um gene em uma célula
WO2014003555A1 (en) 2012-06-27 2014-01-03 Rijksuniversiteit Groningen Improved penicillin production
DK2875119T3 (en) 2012-07-19 2018-04-03 Dsm Ip Assets Bv AGSE-DEFICIENT TRIALS
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
MX370305B (es) 2013-12-02 2019-12-09 Dsm Ip Assets Bv Proteína estructurante de hielo.
MX2017005981A (es) 2014-11-07 2017-06-29 Dupont Nutrition Biosci Aps Celula hospedadora recombinante que expresa actividad de beta-galactosidasa y/o transgalactosidante deficiente en mananasa, celulasa y pectinasa.
EP3242948B1 (de) 2015-01-06 2021-12-22 DSM IP Assets B.V. Crispr-cas-system für eine yarrowia wirtszelle
DK3242949T3 (da) 2015-01-06 2022-01-24 Dsm Ip Assets Bv Crispr-cas-system til en gærværtscelle
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
NZ737305A (en) 2015-06-02 2021-07-30 Dsm Ip Assets Bv Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP3596199A1 (de) 2017-03-13 2020-01-22 DSM IP Assets B.V. Transkriptioneller regulator-defizienz-stamm mit zweikernigem zink-cluster

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8610600D0 (en) * 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.
US4693536A (en) * 1986-06-12 1987-09-15 Molex Incorporated Insulation displacement terminal
EP0383779B2 (de) * 1987-09-04 2000-05-31 Novo Nordisk A/S VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN $i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN $i(ASPERGILLUS)

Also Published As

Publication number Publication date
FI110693B (fi) 2003-03-14
CA1341226C (en) 2001-05-01
PT91453A (pt) 1990-03-08
JP2752714B2 (ja) 1998-05-18
EP0357127B1 (de) 1999-06-09
AU4002189A (en) 1990-02-22
HUT51336A (en) 1990-04-28
GR3030901T3 (en) 1999-11-30
IL91309A0 (en) 1990-03-19
IL91309A (en) 1997-11-20
FI893818A0 (fi) 1989-08-14
AU636572B2 (en) 1993-05-06
PT91453B (pt) 1995-05-31
KR900003366A (ko) 1990-03-26
ATE181111T1 (de) 1999-06-15
DK399889A (da) 1990-02-17
DE68929013D1 (de) 1999-07-15
FI893818A (fi) 1990-02-17
EP0357127A1 (de) 1990-03-07
DK399889D0 (da) 1989-08-15
DE68929013T2 (de) 1999-10-21
JPH02174673A (ja) 1990-07-06
ES2134754T3 (es) 1999-10-16

Similar Documents

Publication Publication Date Title
DK175196B1 (da) Transformeret filamentös fungusvært med en ekspressionskassette, DNA-konstruktion med en sådan ekspressionskassette og fremgangsmåde til fremstilling af et protein under anvendelse af en sådan transformert filamentös fungusvært
US4859596A (en) Cloning system for Kluyveromyces species
JP3068139B2 (ja) 酵母よりヒト血清アルブミンおよびその他の異種蛋白質を微生物学的に生産する方法
Verdoes et al. The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene
Kawahara et al. Endoplasmic reticulum stress-induced mRNA splicing permits synthesis of transcription factor Hac1p/Ern4p that activates the unfolded protein response
Fu et al. Characterization of nit-2, the major nitrogen regulatory gene of Neurospora crassa
Jayaram et al. Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 μm circle
EP0316444B1 (de) Expressionsvektor für hefe
CN113423836A (zh) 重组宿主细胞中碳源调节的蛋白的产生
WO1991013971A1 (en) Neurospora expression system
Gitzinger et al. Functional cross‐kingdom conservation of mammalian and moss (Physcomitrella patens) transcription, translation and secretion machineries
JPH08500014A (ja) Ｋ．ラクチス（Ｋ．ｌａｃｔｉｓ）トランスアルドラーゼ遺伝子のプロモーターおよびその使用
JP2003125791A (ja) 酵母プロモーター
JP2019122386A (ja) 選択的オートファジー経路の不活性化成分を含む糸状真菌細胞及びその使用方法
Dobson et al. Reconstruction of the yeast 2 μ m plasmid partitioning mechanism
US5104795A (en) Shortened phosphoglycerate kinase promoter
JPH06503718A (ja) メチロトローフ酵母細胞におけるヒトリゾチムの産生とその効率的な分泌
JPH08500008A (ja) Ｋ．ラクチス（Ｋ．ｌａｃｔｉｓ）リボソームタンパク質ＲＰ２８の遺伝子のプロモーターおよびその使用
EP0245479B1 (de) Verkürzter phosphoglycerat-kinase-promotor
NO871886L (no) Produksjon av streptokinase ved hjelp av gjaer.
US5541084A (en) Hybrid yeast promoter comprising an ENO2 UAS and TATA region and an additional UAS located between said ENO2 UAS and TATA region
TW394794B (en) D-amino acid oxidase promoter sequence from T.variabilis
KR100726143B1 (ko) 재조합 인체 혈소판 유래 성장인자(ｐｄｇｆ-ｂｂ)를고효율로 생산·분비하는 균주 및 상기 균주의 제작방법
JPH03262487A (ja) ヒト血清アルブミンの安定な製造方法
WO2008020701A1 (en) A new strain highly producing pdgf-bb

Legal Events

Date Code Title Description
PUP Patent expired