ES8301367A1 - Un metodo para realizar un ensayo de ligandos en un medio poroso inerte. - Google Patents

Un metodo para realizar un ensayo de ligandos en un medio poroso inerte.

Info

Publication number
ES8301367A1
ES8301367A1 ES508875A ES508875A ES8301367A1 ES 8301367 A1 ES8301367 A1 ES 8301367A1 ES 508875 A ES508875 A ES 508875A ES 508875 A ES508875 A ES 508875A ES 8301367 A1 ES8301367 A1 ES 8301367A1
Authority
ES
Spain
Prior art keywords
zone
applying
amount
binding material
solid phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES508875A
Other languages
English (en)
Other versions
ES508875A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
American Hospital Supply Corp
Original Assignee
American Hospital Supply Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Hospital Supply Corp filed Critical American Hospital Supply Corp
Publication of ES508875A0 publication Critical patent/ES508875A0/es
Publication of ES8301367A1 publication Critical patent/ES8301367A1/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

UN METODO PARA REALIZAR UN ENSAYO DE LIGANDOS EN UN MEDIO POROSO INERTE. CONSTA DE LAS SIGUIENTES ETAPAS: 1<002> PRECIPITAR E INMOBILIZAR INMUNOLOGICAMENTE UN MATERIAL LIGANTE. 2<002> APLICAR A LA ZONA, BAJO CONDICIONES DE UNION UN ANALITICO DEL QUE ES ESPECIFICO EL MATERIAL LIGANTE. 3<002> APLICAR A LA ZONA UN INDICADOR MARCADO BAJO CONDICIONES QUE PERMITAN QUE EL MISMO RESULTE INMOVILIZADO DENTRO DE DICHA ZONA, EN UNA CANTIDAD QUE PUEDE SER CORRELACIONADA CON LA CANTIDAD DE ANALITO EN LA CITADA ZONA. 4<002> APLICAR AL CENTRO DE DICHA ZONA UNA CORRIENTE DE DISOLVENTE ELUYENTE SUFICIENTE PARA SEPARAR CROMATOGRAFICAMENTE EL INDICADOR QUE NO HA REACCIONADO. 5<002> DETERMINAR LA CANTIDAD DE INDICADOR MARCADO QUE PERMANECE EN LA ZONA.
ES508875A 1981-01-23 1982-01-19 Un metodo para realizar un ensayo de ligandos en un medio poroso inerte. Expired ES8301367A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/227,664 US4517288A (en) 1981-01-23 1981-01-23 Solid phase system for ligand assay

Publications (2)

Publication Number Publication Date
ES508875A0 ES508875A0 (es) 1982-12-01
ES8301367A1 true ES8301367A1 (es) 1982-12-01

Family

ID=22853988

Family Applications (1)

Application Number Title Priority Date Filing Date
ES508875A Expired ES8301367A1 (es) 1981-01-23 1982-01-19 Un metodo para realizar un ensayo de ligandos en un medio poroso inerte.

Country Status (7)

Country Link
US (1) US4517288A (es)
EP (1) EP0070300B1 (es)
CA (1) CA1179940A (es)
DE (1) DE3277704D1 (es)
ES (1) ES8301367A1 (es)
IT (1) IT1147804B (es)
WO (1) WO1982002601A1 (es)

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