JPS57131062A - Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier - Google Patents

Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier

Info

Publication number
JPS57131062A
JPS57131062A JP1501381A JP1501381A JPS57131062A JP S57131062 A JPS57131062 A JP S57131062A JP 1501381 A JP1501381 A JP 1501381A JP 1501381 A JP1501381 A JP 1501381A JP S57131062 A JPS57131062 A JP S57131062A
Authority
JP
Japan
Prior art keywords
antigen
enzyme
antibody
labelled
labelled antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1501381A
Other languages
Japanese (ja)
Other versions
JPH0122905B2 (en
Inventor
Kanefusa Kato
Akira Kosaka
Ryohei Yamamoto
Akira Matsuura
Tadahiko Inukai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AMANO SEIYAKU KK
Amano Enzyme Inc
Original Assignee
AMANO SEIYAKU KK
Amano Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by AMANO SEIYAKU KK, Amano Pharmaceutical Co Ltd filed Critical AMANO SEIYAKU KK
Priority to JP1501381A priority Critical patent/JPS57131062A/en
Publication of JPS57131062A publication Critical patent/JPS57131062A/en
Publication of JPH0122905B2 publication Critical patent/JPH0122905B2/ja
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/538—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by sorbent column, particles or resin strip, i.e. sorbent materials

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To determine an antigen speedily and accurately, by a method wherein free enzyme-labelled antigen is measured using a column of a second antibody insolubilizing carrier. CONSTITUTION:An antigen to be examined and an enzyme-labelled antigen are allowed to compentitively react with antibody immunoglobulin associated with said antigen, and the reaction liquid obtained is made to flow into a column of a second antibody insolubilizing carrier. The obtained conjugate between the enzyme-labelled antigen and the antibody immunoglobulin is attached to the column by adsorption and removed from the reaction liquid. Then the amount of free enzyme-labelled antigen remaining in the liquid phase is obtained by measuring the enzymatic activity and the amount of the antigen is determined from the value measured. Any conventional enzyme such a beta- galactosidase or alkaline phosphateae may be used as labelling enzymes. For the preparation of the enzyme-labelled antigen, a bifunctional reagent such as glutaraldehyde or carbodimide may be used. As an insolubilizing carrier, it is preferable to employ polysaccharides such as agarose or dextran, resins such as polyethylene, or glass, polyacrylamide and the like.
JP1501381A 1981-02-05 1981-02-05 Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier Granted JPS57131062A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1501381A JPS57131062A (en) 1981-02-05 1981-02-05 Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1501381A JPS57131062A (en) 1981-02-05 1981-02-05 Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier

Publications (2)

Publication Number Publication Date
JPS57131062A true JPS57131062A (en) 1982-08-13
JPH0122905B2 JPH0122905B2 (en) 1989-04-28

Family

ID=11876988

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1501381A Granted JPS57131062A (en) 1981-02-05 1981-02-05 Quantitative determination of antigen using enzyme- labelled antigen and second antibody insolubilizing carrier

Country Status (1)

Country Link
JP (1) JPS57131062A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6345561A (en) * 1986-07-24 1988-02-26 マイルス・インコーポレーテッド Enzyme marker antibody reagent having polyalkylene glycol combination group, usage thereof and manufacture thereof
US4980278A (en) * 1985-05-28 1990-12-25 Olympus Optical Co., Ltd. Method of effecting immunological analysis and apparatus for carrying out the same
JPH08145995A (en) * 1994-11-24 1996-06-07 B S R:Kk Method for measuring gallic acid in serum by elisa and method for diagnosing liver disease

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49125517A (en) * 1973-03-19 1974-12-02
JPS51123819A (en) * 1975-04-07 1976-10-28 Summa Corp Fixed immune adsorbing agent
US4104026A (en) * 1976-03-12 1978-08-01 University Of Virginia Immunoassay separation technique
US4128628A (en) * 1976-03-12 1978-12-05 University Of Virginia Alumni Patents Foundation Automated immunoassay
JPS5598359A (en) * 1978-12-22 1980-07-26 Amano Pharmaceut Co Ltd Removing method of non-characteristic obstacle operation in immunity determination method
JPS55136261A (en) * 1979-04-12 1980-10-23 Toyo Jozo Co Ltd Novel compound and kit comprising it

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS49125517A (en) * 1973-03-19 1974-12-02
JPS51123819A (en) * 1975-04-07 1976-10-28 Summa Corp Fixed immune adsorbing agent
US4104026A (en) * 1976-03-12 1978-08-01 University Of Virginia Immunoassay separation technique
US4128628A (en) * 1976-03-12 1978-12-05 University Of Virginia Alumni Patents Foundation Automated immunoassay
JPS5598359A (en) * 1978-12-22 1980-07-26 Amano Pharmaceut Co Ltd Removing method of non-characteristic obstacle operation in immunity determination method
JPS55136261A (en) * 1979-04-12 1980-10-23 Toyo Jozo Co Ltd Novel compound and kit comprising it

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4980278A (en) * 1985-05-28 1990-12-25 Olympus Optical Co., Ltd. Method of effecting immunological analysis and apparatus for carrying out the same
JPS6345561A (en) * 1986-07-24 1988-02-26 マイルス・インコーポレーテッド Enzyme marker antibody reagent having polyalkylene glycol combination group, usage thereof and manufacture thereof
JPH0535990B2 (en) * 1986-07-24 1993-05-27 Miles Inc
JPH08145995A (en) * 1994-11-24 1996-06-07 B S R:Kk Method for measuring gallic acid in serum by elisa and method for diagnosing liver disease

Also Published As

Publication number Publication date
JPH0122905B2 (en) 1989-04-28

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