ES2981865T3 - Conjugado que comprende un péptido de penetración celular y composiciones que comprenden el mismo - Google Patents
Conjugado que comprende un péptido de penetración celular y composiciones que comprenden el mismo Download PDFInfo
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- ES2981865T3 ES2981865T3 ES13816880T ES13816880T ES2981865T3 ES 2981865 T3 ES2981865 T3 ES 2981865T3 ES 13816880 T ES13816880 T ES 13816880T ES 13816880 T ES13816880 T ES 13816880T ES 2981865 T3 ES2981865 T3 ES 2981865T3
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| PCT/KR2013/006218 WO2014010971A1 (ko) | 2012-07-11 | 2013-07-11 | 세포 투과성 펩티드, 그를 포함한 컨쥬게이트 및 그를 포함한 조성물 |
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| US9631184B2 (en) | 2012-09-19 | 2017-04-25 | Gemvax & Kael Co., Ltd. | Cell penetrating peptide, conjugate comprising same, and composition comprising conjugate |
| ES2758451T3 (es) | 2012-09-19 | 2020-05-05 | Gemvax & Kael Co Ltd | Péptido de penetración celular, conjugado que comprende el mismo y composición que comprende el conjugado |
| EP2987497B1 (en) | 2013-04-19 | 2018-12-26 | Gemvax & Kael Co., Ltd. | Composition for treating and preventing ischemic damage |
| AU2014275610B2 (en) | 2013-06-07 | 2018-06-14 | Gemvax & Kael Co., Ltd. | Biological markers useful in cancer immunotherapy |
| JP6495899B2 (ja) | 2013-06-21 | 2019-04-03 | ジェムバックス アンド カエル カンパニー,リミティド | ホルモン分泌調節剤、及びそれを含む組成物 |
| KR101691479B1 (ko) | 2013-10-23 | 2017-01-02 | 주식회사 젬백스앤카엘 | 전립선 비대증 치료 및 예방용 조성물 |
| KR102694658B1 (ko) | 2013-11-22 | 2024-08-14 | 주식회사 젬백스앤카엘 | 혈관 신생 억제 활성을 가지는 펩티드 및 이를 포함하는 조성물 |
| JP6367950B2 (ja) | 2013-12-17 | 2018-08-01 | ジェムバックス アンド カエル カンパニー,リミティド | 前立腺癌治療用組成物 |
| US9937240B2 (en) | 2014-04-11 | 2018-04-10 | Gemvax & Kael Co., Ltd. | Peptide having fibrosis inhibitory activity and composition containing same |
| ES2962532T3 (es) | 2014-04-30 | 2024-03-19 | Gemvax & Kael Co Ltd | Composición para el trasplante de órganos, tejidos o células, kit y procedimiento de trasplante |
| KR102413243B1 (ko) | 2014-12-23 | 2022-06-27 | 주식회사 젬백스앤카엘 | 안질환 치료 펩티드 및 이를 포함하는 안질환 치료용 조성물 |
| JP6751097B2 (ja) | 2015-02-27 | 2020-09-02 | ジェムバックス アンド カエル カンパニー,リミティド | 聴力損傷予防用ペプチド及びそれを含む組成物 |
| KR20250073577A (ko) | 2015-05-26 | 2025-05-27 | 주식회사 젬백스앤카엘 | 신규 펩티드 및 이를 포함한 조성물 |
| KR102638286B1 (ko) | 2015-07-02 | 2024-02-20 | 주식회사 젬백스앤카엘 | 항바이러스 활성 효능을 가지는 펩티드 및 이를 포함하는 조성물 |
| CN105018499A (zh) * | 2015-08-25 | 2015-11-04 | 武汉大学 | 一种抗乙型肝炎病毒的肽核酸及其用途 |
| KR20180064434A (ko) * | 2015-11-03 | 2018-06-14 | 주식회사 젬백스앤카엘 | 신경세포 손실 예방 및 재생 효능을 가지는 펩티드 및 이를 포함하는 조성물 |
| CN109328068A (zh) | 2016-04-07 | 2019-02-12 | 珍白斯凯尔有限公司 | 具有增加端粒酶活性和延长端粒的效果的肽以及包含该肽的组合物 |
| CN109563131A (zh) * | 2016-04-11 | 2019-04-02 | 卡诺有限责任公司 | 手性肽 |
| CN106236695A (zh) * | 2016-06-02 | 2016-12-21 | 哈尔滨源茂达生物技术有限公司 | 一种多肽祛痘修复精华液及其制备方法 |
| US11458208B2 (en) | 2016-06-06 | 2022-10-04 | Asclepiumm Taiwan Co., Ltd | Desmoglein 2 antibody fusion proteins for drug delivery |
| KR101797167B1 (ko) * | 2016-11-09 | 2017-11-13 | 주식회사 바이오셀트란 | 신규의 프로테인 트랜스덕션 도메인 및 이의 용도 |
| CN107759698A (zh) * | 2017-09-18 | 2018-03-06 | 广东工业大学 | 一种egfp‑cta2‑tat融合蛋白在制备荧光探针中的应用 |
| WO2019070962A1 (en) | 2017-10-04 | 2019-04-11 | Ohio State Innovation Foundation | BICYCLIC PEPTIDE INHIBITORS |
| CN117567639A (zh) * | 2017-10-27 | 2024-02-20 | 俄亥俄州国家创新基金会 | 用于细胞内递送装订肽的多肽缀合物 |
| EP3790890A4 (en) | 2018-05-09 | 2022-03-02 | Ohio State Innovation Foundation | CYCLIC PEPTIDES OF CELL PENETRATION WITH ONE OR MORE HYDROPHOBIC RESIDUES |
| CN109553659B (zh) * | 2018-11-26 | 2022-06-21 | 上海华新生物高技术有限公司 | 一种细胞穿透肽及透皮干扰素 |
| KR102351041B1 (ko) * | 2018-12-19 | 2022-01-13 | 한국화학연구원 | 인간 lrrc24 단백질 유래 세포막 투과 도메인 |
| WO2020130547A1 (ko) * | 2018-12-19 | 2020-06-25 | 한국화학연구원 | 인간 엘알알씨24 단백질 유래 세포막 투과 도메인 |
| WO2020141930A1 (ko) * | 2019-01-03 | 2020-07-09 | 김성천 | 피에이치 의존적 막 투과성 펩타이드를 이용한 약물 운반체 및 그것과 약물의 복합체 |
| KR102182985B1 (ko) * | 2019-01-03 | 2020-11-26 | 주식회사 바이오이즈 | pH 의존적 막 투과성 펩타이드를 이용한 약물 운반체 및 그것과 약물의 복합체 |
| CN114007654A (zh) * | 2019-04-17 | 2022-02-01 | 阿迪根有限公司 | 用于分子的细胞内递送的肽和纳米颗粒 |
| EP4058032A4 (en) * | 2019-12-19 | 2024-01-10 | Entrada Therapeutics, Inc. | Compositions for delivery of antisense compounds |
| WO2022012539A1 (zh) * | 2020-07-17 | 2022-01-20 | 养生堂有限公司 | 一种细胞穿膜肽及其应用 |
| US20230279143A1 (en) * | 2020-08-13 | 2023-09-07 | Nibec Co., Ltd. | Protein comprising antibody for targeting oncogenic protein or single chain fragment variable thereof and cancer cell penetrating peptide, and use of the same |
| CN114731988B (zh) * | 2022-05-23 | 2023-10-20 | 昆明市第一人民医院 | 一种hbv感染的树鼩模型构建方法及树鼩模型 |
Family Cites Families (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU207799B (en) | 1991-07-24 | 1993-06-28 | Beres Export Import Rt | Process for producing pharmaceutical composition for influencing the reticuloendothelial system, for treating chronic pain symptomes of degenerative locomotor disorders or tumors, and for treating mucoviscidosis |
| US5885782A (en) * | 1994-09-13 | 1999-03-23 | Nce Pharmaceuticals, Inc. | Synthetic antibiotics |
| CA2261263C (en) | 1996-07-22 | 2008-10-07 | The Victoria University Of Manchester | Use of sex steroid function modulators to treat wounds and fibrotic disorders |
| US6610839B1 (en) | 1997-08-14 | 2003-08-26 | Geron Corporation | Promoter for telomerase reverse transcriptase |
| ATE361099T1 (de) | 1997-05-15 | 2007-05-15 | Chugai Pharmaceutical Co Ltd | Heilmittel für kachexie |
| AU748442B2 (en) | 1997-07-01 | 2002-06-06 | Cambia | Vertebrate telomerase genes and proteins and uses thereof |
| US6639057B1 (en) * | 1998-03-26 | 2003-10-28 | Kyowa Hakko Kogyo Co., Ltd. | Monoclonal antibody against human telomerase catalytic subunit |
| US7030211B1 (en) * | 1998-07-08 | 2006-04-18 | Gemvax As | Antigenic peptides derived from telomerase |
| US6378526B1 (en) | 1998-08-03 | 2002-04-30 | Insite Vision, Incorporated | Methods of ophthalmic administration |
| IL132406A0 (en) | 1998-10-21 | 2001-03-19 | Pfizer Prod Inc | Treatment of bph with cgmp elevators |
| US7078491B1 (en) * | 2000-09-21 | 2006-07-18 | Amgen Inc. | Selective binding agents of telomerase |
| US6815426B2 (en) | 2001-02-16 | 2004-11-09 | E. I. Du Pont De Nemours And Company | Angiogenesis-inhibitory tripeptides, compositions and their methods of use |
| US6967211B2 (en) | 2001-04-10 | 2005-11-22 | Nippon Shinyaku Co. Ltd. | Remedial agent for chronic articular rheumatism |
| ES2334773T3 (es) | 2001-05-16 | 2010-03-16 | Yeda Research And Development Co. Ltd. | Uso de inhibidores de il-18 para el tratamiento o prevencion de la sepsis. |
| WO2003038047A2 (en) | 2001-10-29 | 2003-05-08 | Baylor College Of Medicine | Human telomerase reverse transcriptase as a class-ii restricted tumor-associated antigen |
| US7786084B2 (en) | 2001-12-21 | 2010-08-31 | Biotempt B.V. | Treatment of burns |
| MXPA04009884A (es) | 2002-04-10 | 2004-12-07 | Applied Research Systems | Uso de osteoprotegerina para el tratamiento y/o prevencion de enfermedad fibrotica. |
| KR20050020987A (ko) | 2002-06-12 | 2005-03-04 | 바이오겐 아이덱 엠에이 인코포레이티드 | 아데노신 수용체 길항제를 사용하여 허혈 재관류 손상을치료하는 방법 |
| WO2005000227A2 (en) | 2003-06-06 | 2005-01-06 | Intermune, Inc. | Methods of treating tnf-mediated disorders |
| CA2530900A1 (en) | 2003-06-25 | 2004-12-29 | Canbas Co., Ltd. | Peptides and peptidomimetics having immune-modulating, anti-inflammatory, and anti-viral activity |
| KR20050040517A (ko) | 2003-10-29 | 2005-05-03 | 주식회사 오리엔트 | 허혈성 질환에 대한 저항성을 나타내는 형질전환 생쥐 |
| SI1530965T1 (sl) | 2003-11-11 | 2006-06-30 | Mattern Udo | Formulacija za nasalno aplikacijo z nadzorovanim sproscanjem spolnih hormonov |
| GB0426146D0 (en) | 2004-11-29 | 2004-12-29 | Bioxell Spa | Therapeutic peptides and method |
| WO2006102476A2 (en) | 2005-03-21 | 2006-09-28 | Vicus Therapeutics Spe 1, Llc | Compositions and methods for ameliorating cachexia |
| AR057941A1 (es) | 2005-11-25 | 2007-12-26 | Univ Keio | Agentes terapeuticos para el cancer de prostata |
| AU2006325030B2 (en) | 2005-12-16 | 2012-07-26 | Cellectis | Cell penetrating peptide conjugates for delivering nucleic acids into cells |
| WO2007097561A1 (en) * | 2006-02-20 | 2007-08-30 | Ewha University - Industry Collaboration Foundation | Peptide having cell membrane penetrating activity |
| JP2009544688A (ja) | 2006-07-24 | 2009-12-17 | フォーヒューマンテック カンパニー リミテッド | 虚血性疾患の緩和及び治療のための医薬組成物並びにそれを伝達するための方法 |
| KR20090103957A (ko) | 2007-01-29 | 2009-10-01 | 주식회사 프로셀제약 | 신규한 거대분자 전달 도메인 및 이의 동정 방법 및 용도 |
| KR20120087885A (ko) | 2007-06-29 | 2012-08-07 | 안국약품 주식회사 | 난소암의 예측 마커 |
| CN103298935A (zh) | 2007-08-15 | 2013-09-11 | 阿穆尼克斯公司 | 用于改善生物活性多肽性能的组合物和方法 |
| WO2009025871A1 (en) | 2007-08-23 | 2009-02-26 | University Of Medicine And Dentistry Of Nj | Telomerase reverse transcriptase variant |
| WO2009054996A2 (en) | 2007-10-25 | 2009-04-30 | Genelux Corporation | Systems and methods for viral therapy |
| GB2455539B (en) | 2007-12-12 | 2012-01-18 | Cambridge Entpr Ltd | Anti-inflammatory compositions and combinations |
| TW200946541A (en) | 2008-03-27 | 2009-11-16 | Idenix Pharmaceuticals Inc | Solid forms of an anti-HIV phosphoindole compound |
| HUE030984T2 (en) | 2008-06-16 | 2017-06-28 | Mediolanum Farm S P A | Antitumor immunotherapy |
| US8252282B2 (en) | 2008-06-19 | 2012-08-28 | University Of Medicine & Dentistry Of New Jersey | Nuclear telomerase reverse transcriptase variant |
| ES2334315B1 (es) | 2008-07-29 | 2011-02-28 | Universitat Pompeu Fabra | Peptidos con capacidad de penetracion celular y sus usos. |
| AR072940A1 (es) | 2008-08-20 | 2010-09-29 | Schering Corp | Derivados de piridina y pirimidina sustituidos con etinilo y su uso en el tratamiento de infecciones virales |
| KR20110060940A (ko) | 2008-09-22 | 2011-06-08 | 닛신 파마 가부시키가이샤 | 항염증성 펩티드 |
| WO2010037395A2 (en) * | 2008-10-01 | 2010-04-08 | Dako Denmark A/S | Mhc multimers in cancer vaccines and immune monitoring |
| KR101169030B1 (ko) * | 2009-01-21 | 2012-07-26 | 애니젠 주식회사 | 신규한 세포막 투과 도메인 및 이를 포함하는 세포내 전달 시스템 |
| KR101237927B1 (ko) | 2009-05-07 | 2013-03-04 | (주)문엔제이 | 신경손상 및 신경질환 예방 또는 치료용 약학조성물 |
| EP2251028A1 (en) | 2009-05-12 | 2010-11-17 | Biocompatibles Uk Ltd. | Treatment of eye diseases using encapsulated cells encoding and secreting an anti-angiogenic factor and/or a neuroprotective factor |
| US7928067B2 (en) | 2009-05-14 | 2011-04-19 | Ischemix Llc | Compositions and methods for treating ischemia and ischemia-reperfusion injury |
| RU2548807C2 (ru) | 2009-05-20 | 2015-04-20 | Торэй Индастриз, Инк. | Пептиды, проникающие в клетку |
| KR20110057049A (ko) | 2009-11-23 | 2011-05-31 | 박의신 | 기능성 전립선염 치료제 |
| KR20110062943A (ko) | 2009-12-04 | 2011-06-10 | 주식회사종근당 | 퀴나졸린 유도체를 유효성분으로 하는 전립선 비대증 예방 또는 치료제 |
| EP2524039B1 (en) | 2010-01-11 | 2017-11-29 | CuRNA, Inc. | Treatment of sex hormone binding globulin (shbg) related diseases by inhibition of natural antisense transcript to shbg |
| CN108383894A (zh) | 2010-02-16 | 2018-08-10 | 阿尔特公司 | 多肽及其应用 |
| FR2960542B1 (fr) | 2010-05-27 | 2012-08-17 | Esther Suzy Arlette Fellous | Peptide en tant que medicament, en particulier pour le traitement du cancer |
| KR101263212B1 (ko) * | 2010-05-28 | 2013-05-10 | 성신여자대학교 산학협력단 | 신규한 세포막 투과성 펩타이드 및 그의 용도 |
| WO2011150494A1 (en) | 2010-05-30 | 2011-12-08 | The Governing Council Of The University Of Toronto | Mitochondrial penetrating peptides as carriers for anticancer compounds |
| WO2011155803A2 (ko) | 2010-06-11 | 2011-12-15 | 아주대학교산학협력단 | 청각보호 작용을 하는 신규 화합물 |
| KR101348284B1 (ko) | 2010-09-09 | 2014-01-03 | 주식회사 나이벡 | 인간 유래 세포 투과성 펩타이드와 생리활성 펩타이드 결합체 및 그 용도 |
| US20120208755A1 (en) | 2011-02-16 | 2012-08-16 | Intarcia Therapeutics, Inc. | Compositions, Devices and Methods of Use Thereof for the Treatment of Cancers |
| KR20120121196A (ko) | 2011-04-26 | 2012-11-05 | 주식회사 글루칸 | 관절염 치료제 |
| KR101284772B1 (ko) | 2011-05-24 | 2013-07-17 | 정종문 | 항염증, 진통효과를 가지는 기능성 식품 조성물 |
| KR20120133661A (ko) | 2011-05-31 | 2012-12-11 | 주식회사 바이오포트코리아 | 아스타잔틴을 포함하는 항염증제 |
| KR101288053B1 (ko) | 2011-07-04 | 2013-07-23 | 동국대학교 산학협력단 | 필발 추출물을 유효성분으로 포함하는 내이손상 예방 및 치료용 조성물 |
| KR101361445B1 (ko) | 2011-12-26 | 2014-02-12 | 성균관대학교산학협력단 | 펩타이드, 5-플루오로우라실, 및 성숙수지상세포를 포함하는 암 치료용 약학적 조성물 |
| SG11201404570WA (en) | 2012-02-10 | 2014-11-27 | Hakushinkouseikai Foundation | Proliferating agent for monocyte, culture medium for proliferating monocyte, method for producing monocyte, method for producing dendritic cell, and method for producing dendritic cell vaccine |
| WO2013135266A1 (en) | 2012-03-12 | 2013-09-19 | Gemvax As | Treatment of non-small cell lung carcinoma by active immunotherapy |
| ES2691070T3 (es) | 2012-05-11 | 2018-11-23 | Kael-Gemvax Co.,Ltd | Péptidos antiinflamatorios y composición que comprende los mismos |
| CN104661672B (zh) | 2012-05-11 | 2017-03-08 | 珍白斯凯尔有限公司 | 用于预防或治疗败血症的组合物 |
| US20150125438A1 (en) | 2012-07-20 | 2015-05-07 | Sang Jae Kim | Anti-Inflammatory Peptides and Composition Comprising the Same |
| ES2758451T3 (es) | 2012-09-19 | 2020-05-05 | Gemvax & Kael Co Ltd | Péptido de penetración celular, conjugado que comprende el mismo y composición que comprende el conjugado |
| KR102038487B1 (ko) | 2012-09-19 | 2019-10-30 | 주식회사 젬백스앤카엘 | 텔로머라제 펩티드를 포함하는 항균 또는 항진균용 조성물 |
| US9631184B2 (en) | 2012-09-19 | 2017-04-25 | Gemvax & Kael Co., Ltd. | Cell penetrating peptide, conjugate comprising same, and composition comprising conjugate |
| JP6352923B2 (ja) | 2012-09-19 | 2018-07-04 | ジェムバックス アンド カエル カンパニー,リミティド | 細胞透過性ペプチド、それを含んだコンジュゲート、及びそれを含んだ組成物 |
| KR20140104288A (ko) | 2013-02-20 | 2014-08-28 | 주식회사 카엘젬백스 | Tnf-알파 저해제 |
| SI2959005T1 (sl) | 2013-02-22 | 2022-01-31 | The Board Of Trustees Of The Leland Stanford Junior University | Medicinska uporaba v zvezi z ekstenzijo telomera |
| EP2987497B1 (en) | 2013-04-19 | 2018-12-26 | Gemvax & Kael Co., Ltd. | Composition for treating and preventing ischemic damage |
| AU2014275610B2 (en) | 2013-06-07 | 2018-06-14 | Gemvax & Kael Co., Ltd. | Biological markers useful in cancer immunotherapy |
| JP6495899B2 (ja) | 2013-06-21 | 2019-04-03 | ジェムバックス アンド カエル カンパニー,リミティド | ホルモン分泌調節剤、及びそれを含む組成物 |
| KR101691479B1 (ko) | 2013-10-23 | 2017-01-02 | 주식회사 젬백스앤카엘 | 전립선 비대증 치료 및 예방용 조성물 |
| KR102694658B1 (ko) | 2013-11-22 | 2024-08-14 | 주식회사 젬백스앤카엘 | 혈관 신생 억제 활성을 가지는 펩티드 및 이를 포함하는 조성물 |
| JP6367950B2 (ja) | 2013-12-17 | 2018-08-01 | ジェムバックス アンド カエル カンパニー,リミティド | 前立腺癌治療用組成物 |
| US9937240B2 (en) | 2014-04-11 | 2018-04-10 | Gemvax & Kael Co., Ltd. | Peptide having fibrosis inhibitory activity and composition containing same |
| ES2962532T3 (es) | 2014-04-30 | 2024-03-19 | Gemvax & Kael Co Ltd | Composición para el trasplante de órganos, tejidos o células, kit y procedimiento de trasplante |
| KR102413243B1 (ko) | 2014-12-23 | 2022-06-27 | 주식회사 젬백스앤카엘 | 안질환 치료 펩티드 및 이를 포함하는 안질환 치료용 조성물 |
| JP6751097B2 (ja) | 2015-02-27 | 2020-09-02 | ジェムバックス アンド カエル カンパニー,リミティド | 聴力損傷予防用ペプチド及びそれを含む組成物 |
| KR20180064434A (ko) | 2015-11-03 | 2018-06-14 | 주식회사 젬백스앤카엘 | 신경세포 손실 예방 및 재생 효능을 가지는 펩티드 및 이를 포함하는 조성물 |
| KR20170054310A (ko) | 2015-11-09 | 2017-05-17 | 주식회사 젬백스앤카엘 | 텔로머라제 유래 펩티드를 포함하는 수지상세포 치료제 및 면역 치료제, 및 이를 사용하는 치료방법 |
-
2013
- 2013-07-11 ES ES13816880T patent/ES2981865T3/es active Active
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- 2013-07-11 US US14/413,732 patent/US10967000B2/en active Active
- 2013-07-11 EP EP13816880.2A patent/EP2873678B8/en active Active
- 2013-07-11 WO PCT/KR2013/006218 patent/WO2014010971A1/ko not_active Ceased
- 2013-07-11 CN CN201380044357.3A patent/CN104822698B/zh active Active
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| EP2873678C0 (en) | 2024-06-26 |
| EP2873678B1 (en) | 2024-06-26 |
| JP2015530358A (ja) | 2015-10-15 |
| CN104822698A (zh) | 2015-08-05 |
| US10967000B2 (en) | 2021-04-06 |
| EP2873678A4 (en) | 2016-05-18 |
| EP2873678B8 (en) | 2024-07-17 |
| CN104822698B (zh) | 2018-08-10 |
| KR101799904B1 (ko) | 2017-11-22 |
| EP2873678A1 (en) | 2015-05-20 |
| WO2014010971A1 (ko) | 2014-01-16 |
| JP6272853B2 (ja) | 2018-01-31 |
| KR20150034746A (ko) | 2015-04-03 |
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