ES2963830T3 - Ensayos in vitro y basados en células para medir la actividad de las neurotoxinas botulínicas - Google Patents

Ensayos in vitro y basados en células para medir la actividad de las neurotoxinas botulínicas Download PDF

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Publication number
ES2963830T3
ES2963830T3 ES17801528T ES17801528T ES2963830T3 ES 2963830 T3 ES2963830 T3 ES 2963830T3 ES 17801528 T ES17801528 T ES 17801528T ES 17801528 T ES17801528 T ES 17801528T ES 2963830 T3 ES2963830 T3 ES 2963830T3
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bont
luciferase
cleavage site
single chain
linker
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English (en)
Spanish (es)
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Min Dong
Feifan Yu
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Harvard University
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Harvard University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24069Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES17801528T 2016-10-20 2017-10-19 Ensayos in vitro y basados en células para medir la actividad de las neurotoxinas botulínicas Active ES2963830T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662410558P 2016-10-20 2016-10-20
PCT/US2017/057411 WO2018075783A2 (en) 2016-10-20 2017-10-19 In vitro and cell based assays for measuring the activity of botulinum neurotoxins

Publications (1)

Publication Number Publication Date
ES2963830T3 true ES2963830T3 (es) 2024-04-02

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Family Applications (1)

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ES17801528T Active ES2963830T3 (es) 2016-10-20 2017-10-19 Ensayos in vitro y basados en células para medir la actividad de las neurotoxinas botulínicas

Country Status (21)

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US (2) US11306347B2 (enExample)
EP (1) EP3529616B1 (enExample)
JP (2) JP7227901B2 (enExample)
KR (1) KR102556363B1 (enExample)
CN (1) CN110088624B (enExample)
AU (1) AU2017345560C1 (enExample)
BR (1) BR112019007831A2 (enExample)
CA (1) CA3040507A1 (enExample)
DK (1) DK3529616T5 (enExample)
EA (1) EA039761B1 (enExample)
ES (1) ES2963830T3 (enExample)
FI (1) FI3529616T3 (enExample)
HU (1) HUE064332T2 (enExample)
MX (2) MX2019004431A (enExample)
PL (1) PL3529616T3 (enExample)
PT (1) PT3529616T (enExample)
SA (1) SA519401607B1 (enExample)
SG (1) SG11201903523YA (enExample)
UA (1) UA129533C2 (enExample)
WO (1) WO2018075783A2 (enExample)
ZA (1) ZA201902654B (enExample)

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WO2015134121A2 (en) 2014-01-20 2015-09-11 President And Fellows Of Harvard College Negative selection and stringency modulation in continuous evolution systems
US10920208B2 (en) 2014-10-22 2021-02-16 President And Fellows Of Harvard College Evolution of proteases
US11299729B2 (en) 2015-04-17 2022-04-12 President And Fellows Of Harvard College Vector-based mutagenesis system
US10392674B2 (en) 2015-07-22 2019-08-27 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
US10612011B2 (en) 2015-07-30 2020-04-07 President And Fellows Of Harvard College Evolution of TALENs
EP3365356B1 (en) 2015-10-23 2023-06-28 President and Fellows of Harvard College Nucleobase editors and uses thereof
CN109641941B (zh) * 2016-05-16 2023-06-16 哈佛大学校长及研究员协会 用于纯化和激活肉毒杆菌神经毒素的方法
EA039761B1 (ru) 2016-10-20 2022-03-10 Президент Энд Феллоуз Оф Гарвард Колледж In vitro и клеточные анализы измерения активности ботулинических нейротоксинов
WO2019010164A1 (en) 2017-07-06 2019-01-10 President And Fellows Of Harvard College Evolution of trna synthetases
EP3673059A4 (en) 2017-08-25 2021-09-01 President And Fellows Of Harvard College EVOLUTION OF BONT PEPTIDASES
US11624130B2 (en) 2017-09-18 2023-04-11 President And Fellows Of Harvard College Continuous evolution for stabilized proteins
US11913044B2 (en) 2018-06-14 2024-02-27 President And Fellows Of Harvard College Evolution of cytidine deaminases
WO2021011579A1 (en) * 2019-07-15 2021-01-21 President And Fellows Of Harvard College Evolved botulinum neurotoxins and uses thereof
WO2021195479A1 (en) * 2020-03-26 2021-09-30 Cellex, Inc. Protease assays and their applications
CN114540419A (zh) * 2022-03-04 2022-05-27 中国人民解放军军事科学院军事医学研究院 一种分析包膜病毒膜融合效率的三功能报告系统
GB202213479D0 (en) 2022-09-14 2022-10-26 Ipsen Biopharm Ltd Cell-free clostridial neurotoxin assays
CN121057740A (zh) * 2023-04-21 2025-12-02 深圳琅技生命科技有限公司 一种肽以及含有该肽的发光复合物
GB202404021D0 (en) 2024-03-20 2024-05-01 Ipsen Biopharm Ltd Cell-based neurotoxin assay
CN119039432B (zh) * 2024-10-31 2025-01-28 兰州生物技术开发有限公司 一种检测肉毒毒素活性或突触体相关蛋白的方法和试剂盒

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Also Published As

Publication number Publication date
DK3529616T5 (da) 2024-09-02
PL3529616T3 (pl) 2024-03-18
KR102556363B1 (ko) 2023-07-18
UA129533C2 (uk) 2025-05-28
US20190276873A1 (en) 2019-09-12
EA039761B1 (ru) 2022-03-10
KR20190068582A (ko) 2019-06-18
EP3529616B1 (en) 2023-09-06
CA3040507A1 (en) 2018-04-26
SG11201903523YA (en) 2019-05-30
AU2017345560A1 (en) 2019-05-02
US11306347B2 (en) 2022-04-19
US11788069B2 (en) 2023-10-17
AU2017345560C1 (en) 2025-04-17
PT3529616T (pt) 2023-12-06
JP2019532651A (ja) 2019-11-14
WO2018075783A3 (en) 2018-06-21
FI3529616T3 (fi) 2023-12-04
ZA201902654B (en) 2024-08-28
AU2017345560B2 (en) 2024-12-12
EP3529616A2 (en) 2019-08-28
MX2023004733A (es) 2023-05-10
DK3529616T3 (da) 2023-12-11
JP2023011700A (ja) 2023-01-24
CN110088624B (zh) 2024-01-16
HUE064332T2 (hu) 2024-03-28
SA519401607B1 (ar) 2022-08-04
US20220356508A1 (en) 2022-11-10
BR112019007831A2 (pt) 2019-10-01
WO2018075783A2 (en) 2018-04-26
CN110088624A (zh) 2019-08-02
JP7227901B2 (ja) 2023-02-22
MX2019004431A (es) 2019-08-14
EA201990929A1 (ru) 2019-10-31

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