ES2940071T3 - Componentes y procedimientos de amplificación isotérmica - Google Patents

Componentes y procedimientos de amplificación isotérmica Download PDF

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ES2940071T3
ES2940071T3 ES20182776T ES20182776T ES2940071T3 ES 2940071 T3 ES2940071 T3 ES 2940071T3 ES 20182776 T ES20182776 T ES 20182776T ES 20182776 T ES20182776 T ES 20182776T ES 2940071 T3 ES2940071 T3 ES 2940071T3
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nucleic acid
sequence
primer
amplification
cases
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Andrew P Miller
Honghua Zhang
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National Diagnostics Inc
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National Diagnostics Inc
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
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    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/107RNA dependent DNA polymerase,(i.e. reverse transcriptase)
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    • C12Q2527/101Temperature
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    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR
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    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/113Real time assay
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432Quenching

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Tires In General (AREA)
  • Amplifiers (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
ES20182776T 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica Active ES2940071T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15/090,405 US9617587B1 (en) 2016-04-04 2016-04-04 Isothermal amplification components and processes

Publications (1)

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ES2940071T3 true ES2940071T3 (es) 2023-05-03

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ES20182776T Active ES2940071T3 (es) 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica
ES22165594T Active ES2988526T3 (es) 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica
ES17711527T Active ES2881509T3 (es) 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica

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ES22165594T Active ES2988526T3 (es) 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica
ES17711527T Active ES2881509T3 (es) 2016-04-04 2017-03-06 Componentes y procedimientos de amplificación isotérmica

Country Status (8)

Country Link
US (4) US9617587B1 (https=)
EP (4) EP3760735B1 (https=)
JP (4) JP6758474B2 (https=)
CN (1) CN109196114A (https=)
AU (2) AU2017247133B2 (https=)
CA (1) CA3018202A1 (https=)
ES (3) ES2940071T3 (https=)
WO (1) WO2017176404A1 (https=)

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