ES2702621T3 - Microorganismos recombinantes y sus usos - Google Patents
Microorganismos recombinantes y sus usos Download PDFInfo
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- ES2702621T3 ES2702621T3 ES12749472T ES12749472T ES2702621T3 ES 2702621 T3 ES2702621 T3 ES 2702621T3 ES 12749472 T ES12749472 T ES 12749472T ES 12749472 T ES12749472 T ES 12749472T ES 2702621 T3 ES2702621 T3 ES 2702621T3
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- microorganism
- acetone
- isopropanol
- clostridium
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
- C12P7/28—Acetone-containing products
- C12P7/30—Acetone-containing products produced from substrate containing inorganic compounds other than water
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
Landscapes
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- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
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| NZ700609A (en) * | 2012-04-05 | 2016-07-29 | Lanzatech New Zealand Ltd | Enzyme-altered metabolite activity |
| US20130316364A1 (en) | 2012-05-23 | 2013-11-28 | Lanzatech New Zealand Limited | Selection Method and Recombinant Microorganisms and uses Therefor |
| KR102098849B1 (ko) | 2012-05-30 | 2020-04-09 | 란자테크 뉴질랜드 리미티드 | 재조합 미생물 및 이의 용도 |
| US20130323820A1 (en) | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
| EP2859089B1 (en) | 2012-06-08 | 2017-03-22 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
| US20150079650A1 (en) * | 2013-06-05 | 2015-03-19 | Lanzatech New Zealand Limited | Recombinant microorganisms exhibiting increased flux through a fermentation pathway |
| EA033071B1 (ru) * | 2013-09-22 | 2019-08-30 | Ланцатек Нью Зилэнд Лимитед | Способ увеличения выхода продуктов из ацетолактата |
| JP2016538869A (ja) * | 2013-12-03 | 2016-12-15 | ランザテク・ニュージーランド・リミテッド | ケトンの生成のための微生物及び方法 |
| AU2015211015B2 (en) | 2014-01-28 | 2018-11-15 | Lanzatech Nz, Inc. | Method of producing a recombinant microorganism |
| CN110066718B (zh) | 2014-10-22 | 2022-09-20 | 朗泽科技新西兰有限公司 | 多阶段系统和用于将co转化成乙醇的多阶段生物学方法 |
| WO2016065085A1 (en) | 2014-10-22 | 2016-04-28 | Lanzatech New Zealand Limited | Gas testing unit and method |
| EA035950B1 (ru) | 2014-12-08 | 2020-09-04 | Ланцатек Нью Зилэнд Лимитед | Рекомбинантные микроорганизмы, проявляющие повышенный поток при ферментационном пути |
| TWI739734B (zh) | 2015-02-23 | 2021-09-21 | 紐西蘭商藍瑟科技紐西蘭有限公司 | 用於將甲烷轉化為產物之重組產醋酸細菌 |
| JP6905518B2 (ja) | 2015-10-13 | 2021-07-21 | ランザテク・ニュージーランド・リミテッド | エネルギー発生発酵経路を含む遺伝子操作細菌 |
| KR20180127632A (ko) | 2015-12-03 | 2018-11-29 | 란자테크 뉴질랜드 리미티드 | 가스 발효 아세토젠에서의 효율을 개선하기 위한 아르기닌 보충 |
| US10358661B2 (en) | 2015-12-28 | 2019-07-23 | Lanzatech New Zealand Limited | Microorganism with modified hydrogenase activity |
| KR20180118651A (ko) | 2016-02-01 | 2018-10-31 | 란자테크 뉴질랜드 리미티드 | 통합형 발효 및 전해 공정 |
| EP3420089B1 (en) | 2016-02-26 | 2021-12-29 | LanzaTech NZ, Inc. | Crispr/cas systems for c-1 fixing bacteria |
| JP7502029B2 (ja) | 2016-05-14 | 2024-06-18 | ランザテク，インコーポレイテッド | 修飾されたアルデヒド：フェレドキシンオキシドレダクターゼ活性を有する微生物および関連方法 |
| AU2018329846B2 (en) | 2017-09-08 | 2024-01-11 | Lanzatech, Inc. | Processes and systems for metabolite production using hydrogen rich C1-containing substrates |
| AU2019218389B2 (en) | 2018-02-12 | 2024-09-05 | Lanzatech, Inc. | A process for improving carbon conversion efficiency |
| AU2019257224B2 (en) | 2018-04-20 | 2024-12-19 | Lanzatech, Inc. | Intermittent electrolysis streams |
| KR102549843B1 (ko) | 2018-11-19 | 2023-06-29 | 란자테크, 인크. | 발효와 가스화의 통합 |
| US11091415B2 (en) | 2019-02-08 | 2021-08-17 | Lanzatech, Inc. | Process for recovering close boiling products |
| WO2020188033A1 (en) | 2019-03-20 | 2020-09-24 | Global Bioenergies | Improved means and methods for producing isobutene from acetyl-coa |
| KR20240158377A (ko) | 2019-07-11 | 2024-11-04 | 란자테크, 인크. | 가스 이용률을 최적화하는 방법 |
| CN111057686B (zh) * | 2019-12-23 | 2021-05-04 | 浙江大学 | 一种醇脱氢酶突变体及应用 |
| US11932818B2 (en) | 2020-03-16 | 2024-03-19 | Lanzatech, Inc. | Tail gas of gas fermentation to dry gasification feedstock |
| BR112022018609A2 (pt) | 2020-03-18 | 2022-11-08 | Lanzatech Inc | Microrganismo capaz de produzir 2-feniletanol, e, método de produção de 2-feniletanol |
| MY197253A (en) * | 2020-06-06 | 2023-06-08 | Lanzatech Inc | Microorganism with knock-in at acetolactate decarboxylase gene locus |
| JP2023542338A (ja) * | 2020-09-25 | 2023-10-06 | ランザテク，インコーポレイテッド | 組換え微生物及びそのための使用 |
| AU2022218205A1 (en) | 2021-02-08 | 2023-05-25 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| WO2022217282A1 (en) | 2021-04-09 | 2022-10-13 | Lanzatech, Inc. | Process and apparatus for providing a feedstock |
| CN114561416B (zh) * | 2022-04-27 | 2022-07-15 | 深圳中科翎碳生物科技有限公司 | 利用电能固定co2及合成异丙醇的工程菌及构建方法 |
| WO2024036187A1 (en) | 2022-08-10 | 2024-02-15 | Lanzatech, Inc. | Carbon sequestration in soils with production of chemical products |
| WO2024231219A1 (en) | 2023-05-11 | 2024-11-14 | Evonik Operations Gmbh | Biotechnological production of acetone |
| CN117625571B (zh) * | 2023-12-05 | 2025-06-10 | 迪嘉药业集团股份有限公司 | 一种改进的酮还原酶及其在依折麦布制备中的应用 |
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| US5173429A (en) | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
| UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
| NZ523484A (en) | 2000-07-25 | 2005-01-28 | Emmaus Foundation Inc | Methods for increasing the production of ethanol from microbial fermentation |
| NZ566406A (en) * | 2005-10-26 | 2012-04-27 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| NZ546496A (en) * | 2006-04-07 | 2008-09-26 | Lanzatech New Zealand Ltd | Gas treatment process |
| US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
| AU2008310573A1 (en) * | 2007-10-12 | 2009-04-16 | The Regents Of The University Of California | Microorganism engineered to produce isopropanol |
| EP2217696B1 (en) | 2007-11-13 | 2015-09-16 | Lanzatech New Zealand Limited | Novel bacteria and methods of use thereof |
| KR20130084704A (ko) * | 2008-03-12 | 2013-07-25 | 란자테크 뉴질랜드 리미티드 | 미생물 알콜 생산 방법 |
| CN102016006B (zh) * | 2008-04-25 | 2013-04-10 | 财团法人地球环境产业技术研究机构 | 具有异丙醇生产能力的棒状细菌转化体 |
| KR20110097951A (ko) * | 2008-12-16 | 2011-08-31 | 게노마티카 인코포레이티드 | 합성가스와 다른 탄소원을 유용 제품으로 전환시키기 위한 미생물 및 방법 |
| DE102009002583A1 (de) * | 2009-04-23 | 2010-10-28 | Evonik Degussa Gmbh | Zellen und Verfahren zur Herstellung von Aceton |
| CA2772081A1 (en) * | 2009-08-21 | 2011-02-24 | Mascoma Corporation | Production of propanols, alcohols, and polyols in consolidated bioprocessing organisms |
| US8143037B2 (en) | 2010-03-19 | 2012-03-27 | Coskata, Inc. | Ethanologenic Clostridium species, Clostridium coskatii |
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| EP2678432A4 (en) | 2016-06-22 |
| KR20190097292A (ko) | 2019-08-20 |
| WO2012115527A2 (en) | 2012-08-30 |
| CA2825267A1 (en) | 2012-08-30 |
| TW201309795A (zh) | 2013-03-01 |
| JP2014506478A (ja) | 2014-03-17 |
| CA2995872A1 (en) | 2012-08-30 |
| JP6199747B2 (ja) | 2017-09-20 |
| CA2995872C (en) | 2019-09-17 |
| KR102121886B1 (ko) | 2020-06-15 |
| CA2825267C (en) | 2018-04-03 |
| MY193665A (en) | 2022-10-24 |
| EP2678432B1 (en) | 2018-08-01 |
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