ES2702621T3 - Microorganismos recombinantes y sus usos - Google Patents
Microorganismos recombinantes y sus usos Download PDFInfo
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- ES2702621T3 ES2702621T3 ES12749472T ES12749472T ES2702621T3 ES 2702621 T3 ES2702621 T3 ES 2702621T3 ES 12749472 T ES12749472 T ES 12749472T ES 12749472 T ES12749472 T ES 12749472T ES 2702621 T3 ES2702621 T3 ES 2702621T3
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- microorganism
- acetone
- isopropanol
- clostridium
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/10—Transferases (2.)
- C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
- C12P7/26—Ketones
- C12P7/28—Acetone-containing products
- C12P7/30—Acetone-containing products produced from substrate containing inorganic compounds other than water
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
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- C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
- C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
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- C—CHEMISTRY; METALLURGY
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- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
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- C12Y—ENZYMES
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- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
- Y02P20/133—Renewable energy sources, e.g. sunlight
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- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US20130316364A1 (en) | 2012-05-23 | 2013-11-28 | Lanzatech New Zealand Limited | Selection Method and Recombinant Microorganisms and uses Therefor |
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| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
| WO2014197746A1 (en) * | 2013-06-05 | 2014-12-11 | Lanzatech New Zealand Limited | Recombinant microorganisms exhibiting increased flux through a fermentation pathway |
| DK3047028T5 (da) * | 2013-09-22 | 2024-10-14 | Lanzatech Nz Inc | Fermenteringsfremgangsmåde |
| US20150152445A1 (en) * | 2013-12-03 | 2015-06-04 | Lanzatech New Zealand Limited | Microorganisms and methods for the production of ketones |
| EP3099779B1 (en) | 2014-01-28 | 2020-09-02 | LanzaTech New Zealand Limited | Method of producing a recombinant microorganism |
| TWI689592B (zh) | 2014-10-22 | 2020-04-01 | 紐西蘭商藍瑟科技紐西蘭有限公司 | 多段生物反應器方法 |
| WO2016065085A1 (en) | 2014-10-22 | 2016-04-28 | Lanzatech New Zealand Limited | Gas testing unit and method |
| AU2015360786B2 (en) | 2014-12-08 | 2021-10-21 | Lanzatech Nz, Inc. | Recombinant microorganisms exhibiting increased flux through a fermentation pathway |
| WO2016138050A1 (en) | 2015-02-23 | 2016-09-01 | Lanzatech New Zealand Limited | Recombinant acetogenic bacterium for the conversion of methane to products |
| JP6905518B2 (ja) | 2015-10-13 | 2021-07-21 | ランザテク・ニュージーランド・リミテッド | エネルギー発生発酵経路を含む遺伝子操作細菌 |
| CA3010412C (en) | 2015-12-03 | 2020-04-14 | Lanzatech New Zealand Limited | Arginine supplementation to improve efficiency in gas fermenting acetogens |
| US10358661B2 (en) | 2015-12-28 | 2019-07-23 | Lanzatech New Zealand Limited | Microorganism with modified hydrogenase activity |
| US10358662B2 (en) | 2016-02-01 | 2019-07-23 | Lanzatech New Zealand Limited | Integrated fermentation and electrolysis process |
| SG11201807025SA (en) | 2016-02-26 | 2018-09-27 | Lanzatech New Zealand Ltd | Crispr/cas systems for c-1 fixing bacteria |
| KR20230044031A (ko) | 2016-05-14 | 2023-03-31 | 란자테크, 인크. | 알데히드:페레독신 옥시도리덕타제 활성이 변경된 미생물 및 관련 방법 |
| CN117551705A (zh) | 2017-09-08 | 2024-02-13 | 朗泽科技有限公司 | 用于使用富氢气的含有c1的底物生产代谢物的方法和系统 |
| CA3090411A1 (en) | 2018-02-12 | 2019-08-15 | Lanzatech, Inc. | A process for improving carbon conversion efficiency |
| MY204224A (en) | 2018-04-20 | 2024-08-16 | Lanzatech Inc | Intermittent electrolysis streams |
| CA3120151C (en) | 2018-11-19 | 2024-04-30 | Lanzatech, Inc. | Integration of fermentation and gasification |
| US11091415B2 (en) | 2019-02-08 | 2021-08-17 | Lanzatech, Inc. | Process for recovering close boiling products |
| CN113614240B (zh) | 2019-03-20 | 2024-12-13 | 环球生物能源公司 | 用于从乙酰-CoA生产异丁烯的改进手段和方法 |
| KR20240158377A (ko) | 2019-07-11 | 2024-11-04 | 란자테크, 인크. | 가스 이용률을 최적화하는 방법 |
| CN111057686B (zh) * | 2019-12-23 | 2021-05-04 | 浙江大学 | 一种醇脱氢酶突变体及应用 |
| US11932818B2 (en) | 2020-03-16 | 2024-03-19 | Lanzatech, Inc. | Tail gas of gas fermentation to dry gasification feedstock |
| AU2021237368B2 (en) | 2020-03-18 | 2025-05-08 | Lanzatech, Inc. | Fermentative production of 2-phenylethanol from gaseous substrates |
| JP2023520257A (ja) * | 2020-06-06 | 2023-05-16 | ランザテク，インコーポレイテッド | アセトラクテートデカルボキシラーゼ遺伝子座位でのノックインを有する微生物 |
| TW202212566A (zh) * | 2020-09-25 | 2022-04-01 | 美商朗澤科技有限公司 | 重組微生物及其用途 |
| AU2022218205A1 (en) | 2021-02-08 | 2023-05-25 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| WO2022217284A1 (en) | 2021-04-09 | 2022-10-13 | Lanzatech, Inc. | Flexible fermentation platform for improved conversion of carbon dioxide into products |
| WO2022217282A1 (en) | 2021-04-09 | 2022-10-13 | Lanzatech, Inc. | Process and apparatus for providing a feedstock |
| CN114561416B (zh) * | 2022-04-27 | 2022-07-15 | 深圳中科翎碳生物科技有限公司 | 利用电能固定co2及合成异丙醇的工程菌及构建方法 |
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| CN121464221A (zh) | 2023-05-11 | 2026-02-03 | 赢创运营有限公司 | 丙酮的生物技术生产 |
| CN117625571B (zh) * | 2023-12-05 | 2025-06-10 | 迪嘉药业集团股份有限公司 | 一种改进的酮还原酶及其在依折麦布制备中的应用 |
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| US5173429A (en) | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
| UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
| JP4883872B2 (ja) | 2000-07-25 | 2012-02-22 | エモース・フアンデーシヨン・インコーポレーテツド | 微生物発酵によるエタノール生産を向上させる方法 |
| EP1948814B1 (en) * | 2005-10-26 | 2018-11-21 | Butamax (TM) Advanced Biofuels LLC | Fermentive production of four carbon alcohols |
| NZ546496A (en) * | 2006-04-07 | 2008-09-26 | Lanzatech New Zealand Ltd | Gas treatment process |
| US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
| JP2011500031A (ja) * | 2007-10-12 | 2011-01-06 | ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア | イソプロパノールを生産するように操作された微生物 |
| KR101375029B1 (ko) * | 2007-11-13 | 2014-03-14 | 란자테크 뉴질랜드 리미티드 | 신규 세균 및 이의 이용 방법 |
| US8119378B2 (en) * | 2008-03-12 | 2012-02-21 | Lanzatech New Zealand Limited | Microbial alcohol production process |
| EP2270135A4 (en) | 2008-04-25 | 2013-04-10 | Res Inst Innovative Tech Earth | GENETICALLY MODIFIED CORYNEFORME BACTERIA FOR THE MANUFACTURE OF ISOPROPANOL |
| KR20110097951A (ko) * | 2008-12-16 | 2011-08-31 | 게노마티카 인코포레이티드 | 합성가스와 다른 탄소원을 유용 제품으로 전환시키기 위한 미생물 및 방법 |
| DE102009002583A1 (de) * | 2009-04-23 | 2010-10-28 | Evonik Degussa Gmbh | Zellen und Verfahren zur Herstellung von Aceton |
| CN102666862A (zh) * | 2009-08-21 | 2012-09-12 | 马斯科马公司 | 在联合生物加工生物体中生产丙醇、醇和多元醇 |
| US8143037B2 (en) | 2010-03-19 | 2012-03-27 | Coskata, Inc. | Ethanologenic Clostridium species, Clostridium coskatii |
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| CN103502435A (zh) | 2014-01-08 |
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| EP3401405A1 (en) | 2018-11-14 |
| WO2012115527A2 (en) | 2012-08-30 |
| AU2012221176B2 (en) | 2015-03-19 |
| BR112013021524A2 (pt) | 2017-06-13 |
| CN103502435B (zh) | 2018-05-18 |
| CA2825267A1 (en) | 2012-08-30 |
| TWI541348B (zh) | 2016-07-11 |
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