BR112016012634A2 - bactéria carboxidotrófica acetogênica recombinante, e, métodos para produzir acetona e mek - Google Patents

bactéria carboxidotrófica acetogênica recombinante, e, métodos para produzir acetona e mek

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Publication number
BR112016012634A2
BR112016012634A2 BR112016012634A BR112016012634A BR112016012634A2 BR 112016012634 A2 BR112016012634 A2 BR 112016012634A2 BR 112016012634 A BR112016012634 A BR 112016012634A BR 112016012634 A BR112016012634 A BR 112016012634A BR 112016012634 A2 BR112016012634 A2 BR 112016012634A2
Authority
BR
Brazil
Prior art keywords
alcohol dehydrogenase
secondary alcohol
mek
methods
acetone
Prior art date
Application number
BR112016012634A
Other languages
English (en)
Inventor
Paul Mueller Alexander
Al-Sinawi Bakir
Koepke Michael
Overgaard Jensen Rasmus
Edward Hill Ryan
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of BR112016012634A2 publication Critical patent/BR112016012634A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y208/00—Transferases transferring sulfur-containing groups (2.8)
    • C12Y208/03—CoA-transferases (2.8.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01028—Propanediol dehydratase (4.2.1.28)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção fornece uma bactéria carboxidotrófica, acetogênica recombinante que não tem álcool desidrogenase secundária ou compreende umo álcool desidrogenase secundária inativada. o álcool desidrogenase secundária inativada pode ser codificada por um gene de álcool desidrogenase secundária que compreende uma mutação de inativação que reduz a capacidade da bactéria de converter acetona em isopropanol e de converter metil etil cetona em 2-butanol. visto que uma bactéria que não tem álcool desidrogenase secundária ou compreende umo álcool desidrogenase secundária inativada acumula compostos que contêm carbonila, a invenção fornece também um método de produzir compostos que contêm carbonila, tais como acetona e metil etil cetona.
BR112016012634A 2013-12-03 2014-12-03 bactéria carboxidotrófica acetogênica recombinante, e, métodos para produzir acetona e mek BR112016012634A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361911449P 2013-12-03 2013-12-03
PCT/US2014/068463 WO2015085015A1 (en) 2013-12-03 2014-12-03 Microorganisms and methods for the production of ketones

Publications (1)

Publication Number Publication Date
BR112016012634A2 true BR112016012634A2 (pt) 2017-09-26

Family

ID=53264867

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016012634A BR112016012634A2 (pt) 2013-12-03 2014-12-03 bactéria carboxidotrófica acetogênica recombinante, e, métodos para produzir acetona e mek

Country Status (8)

Country Link
US (1) US20150152445A1 (pt)
EP (1) EP3077502A1 (pt)
JP (1) JP2016538869A (pt)
KR (1) KR20160093648A (pt)
CN (1) CN105874057A (pt)
BR (1) BR112016012634A2 (pt)
CA (1) CA2931627A1 (pt)
WO (1) WO2015085015A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016138372A1 (en) * 2015-02-27 2016-09-01 White Dog Labs, Inc. Mixotrophic fermentation method for making acetone, isopropanol, butyric acid and other bioproducts, and mixtures thereof
KR20210037002A (ko) 2015-10-13 2021-04-05 란자테크 뉴질랜드 리미티드 에너지-생성 발효 경로를 포함하는 유전자 조작된 박테리아
CA3053053C (en) * 2017-03-20 2020-04-14 Lanzatech, Inc. A process and system for product recovery and cell recycle
JP7124075B2 (ja) * 2017-11-09 2022-08-23 ブラスケム・エス・エー 発酵からケトン及びグリコールを回収する方法
BR112021015449A2 (pt) 2019-02-08 2021-10-05 Lanzatech, Inc. Métodos para recuperar produto a partir de um caldo de fermentação e para recuperar produto a partir de uma corrente enriquecida com produto
MY197253A (en) * 2020-06-06 2023-06-08 Lanzatech Inc Microorganism with knock-in at acetolactate decarboxylase gene locus

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101437948A (zh) * 2006-05-02 2009-05-20 纳幕尔杜邦公司 四碳醇的发酵生产
US8962298B2 (en) * 2006-05-02 2015-02-24 Butamax Advanced Biofuels Llc Recombinant host cell comprising a diol dehydratase
US8039239B2 (en) * 2008-12-16 2011-10-18 Coskata, Inc. Recombinant microorganisms having modified production of alcohols and acids
KR102015672B1 (ko) * 2011-02-25 2019-08-29 란자테크 뉴질랜드 리미티드 재조합 미생물 및 이의 용도
EA031093B1 (ru) * 2012-01-31 2018-11-30 Ланцатек Нью Зилэнд Лимитед Рекомбинантный карбоксидотрофный ацетогенный микроорганизм, способ его получения и способ получения продуктов с его использованием

Also Published As

Publication number Publication date
CA2931627A1 (en) 2015-06-11
CN105874057A (zh) 2016-08-17
EP3077502A1 (en) 2016-10-12
JP2016538869A (ja) 2016-12-15
US20150152445A1 (en) 2015-06-04
WO2015085015A1 (en) 2015-06-11
KR20160093648A (ko) 2016-08-08

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal acc. article 33 of ipl - extension of time limit for request of examination expired
B350 Update of information on the portal [chapter 15.35 patent gazette]