ES2659730T3 - Nuevas enzimas de metanol deshidrogenasas procedentes de Bacillus - Google Patents

Nuevas enzimas de metanol deshidrogenasas procedentes de Bacillus Download PDF

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Publication number
ES2659730T3
ES2659730T3 ES13701767.9T ES13701767T ES2659730T3 ES 2659730 T3 ES2659730 T3 ES 2659730T3 ES 13701767 T ES13701767 T ES 13701767T ES 2659730 T3 ES2659730 T3 ES 2659730T3
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mga3
mdh
mdh2
seq
mdh3
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ES13701767.9T
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English (en)
Spanish (es)
Inventor
Trygve Brautaset
Tonje Marita Bjerkan HEGGESET
Anne KROG
Wilhelmus Johannes Quax
Mark Jan Jacobus Bernhard SIBBALD
Julia VORHOLT
Jonas Müller
Patrick Kiefer
Eva POTTHOFF
Volker F Wendisch
Lennart LESSMEIER
Stéphanie HEUX
Jean-Charles PORTAIS
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Eidgenoessische Technische Hochschule Zurich ETHZ
Rijksuniversiteit Groningen
Sintef Tto AS
Original Assignee
Eidgenoessische Technische Hochschule Zurich ETHZ
Rijksuniversiteit Groningen
Sintef Tto AS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01244—Methanol dehydrogenase (1.1.1.244)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES13701767.9T 2012-01-25 2013-01-25 Nuevas enzimas de metanol deshidrogenasas procedentes de Bacillus Active ES2659730T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201201178 2012-01-25
GBGB1201178.9A GB201201178D0 (en) 2012-01-25 2012-01-25 Novel enzymes
PCT/EP2013/051516 WO2013110797A1 (en) 2012-01-25 2013-01-25 Novel methanol dehydrogenase enzymes from bacillus

Publications (1)

Publication Number Publication Date
ES2659730T3 true ES2659730T3 (es) 2018-03-19

Family

ID=45840881

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13701767.9T Active ES2659730T3 (es) 2012-01-25 2013-01-25 Nuevas enzimas de metanol deshidrogenasas procedentes de Bacillus

Country Status (10)

Country Link
US (1) US9322000B2 (ru)
EP (1) EP2807253B1 (ru)
JP (1) JP6185484B2 (ru)
KR (1) KR102114507B1 (ru)
CN (1) CN104220591B (ru)
CA (1) CA2862558C (ru)
ES (1) ES2659730T3 (ru)
GB (1) GB201201178D0 (ru)
RU (1) RU2624017C2 (ru)
WO (1) WO2013110797A1 (ru)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8349587B2 (en) 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds
US9657316B2 (en) * 2012-08-27 2017-05-23 Genomatica, Inc. Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing 1,4-butanediol related thereto
KR20150100666A (ko) * 2012-12-27 2015-09-02 세키스이가가쿠 고교가부시키가이샤 재조합 세포, 및 이소프렌의 생산 방법
EP2947143B1 (en) * 2013-01-21 2018-07-11 Sekisui Chemical Co., Ltd. Recombinant cell, and method for producing 1,4-butanediol
WO2015051298A2 (en) * 2013-10-04 2015-04-09 Genomatica, Inc. Alcohol dehydrogenase variants
PL3077501T3 (pl) 2013-12-03 2022-01-31 Genomatica, Inc. Mikroorganizmy i sposoby poprawy wydajności produktu na metanolu z użyciem syntezy acetylo-coa
WO2015108777A1 (en) 2014-01-16 2015-07-23 Papoutsakis Eleftherios T Synthetic methylotrophy to liquid fuels and chemicals
KR20150101752A (ko) * 2014-02-27 2015-09-04 한국생명공학연구원 증대된 환원활성을 나타내는 변이 메탄올 탈수소화 효소
WO2015160848A1 (en) * 2014-04-15 2015-10-22 Industrial Microbes, Inc. Synthetic methanotrophic and methylotrophic microorganisms
CN115322261A (zh) 2015-10-30 2022-11-11 基因组股份公司 甲醇脱氢酶融合蛋白
CA3005460A1 (en) 2015-11-18 2017-05-26 Industrial Microbes, Inc. Functional expression of monooxygenases and methods of use
CN107227286B (zh) * 2017-06-13 2020-11-03 南京工业大学 一株高产琥珀酸的基因工程菌及其构建方法与应用
CN107267472B (zh) * 2017-06-21 2020-11-10 南京工业大学 提高大肠杆菌甲醇代谢途径中限速酶活性的方法
EP3642324A1 (en) 2017-06-23 2020-04-29 Institut National des Sciences Appliquées de Toulouse Synthetic methylotrophy
WO2020006058A2 (en) 2018-06-26 2020-01-02 Genomatica, Inc. Engineered microorganisms with g3p---> 3pg enzyme and/or fructose-1,6-bisphosphatase including those having synthetic or enhanced methylotrophy
JP2022529690A (ja) * 2019-04-19 2022-06-23 ギンゴー バイオワークス， インコーポレイテッド メタノールの利用
CN116355919B (zh) * 2021-12-28 2024-03-19 天津大学 高产黄素单核苷酸的大肠杆菌菌株及构建方法与用途

Family Cites Families (13)

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BR9006409A (pt) * 1989-04-10 1991-08-06 Univ Minnesota Producao de aminoacidos por bacilo metilotrofico
US6261825B1 (en) 1989-04-10 2001-07-17 Regents Of The University Of Minnesota Production of amino acids using auxotrophic mutants of methylotrophic bacillus
US6331428B1 (en) 1997-07-09 2001-12-18 Ajinomoto Co., Inc. Hexulose phosphate isomerase gene
US6083728A (en) * 1997-10-17 2000-07-04 Regents Of The University Of Minnesota Production of glutamate using wild type Bacillus methanolicus
JP2000069976A (ja) * 1998-09-02 2000-03-07 Ajinomoto Co Inc メタノール脱水素酵素の新規活性促進因子及びその遺伝子
UA67804C2 (ru) 1998-10-02 2004-07-15 Роналд Нортедж Клапан
JP2001057896A (ja) * 1999-06-15 2001-03-06 Ajinomoto Co Inc Ｌ−リジンの製造法
JP2002017351A (ja) 2000-07-07 2002-01-22 Ajinomoto Co Inc ホスホヘキシュロイソメラーゼ及びその遺伝子
EP1266966B1 (en) 2001-06-12 2009-04-29 Ajinomoto Co., Inc. Method for producing L-lysine or L-arginine by using methanol assimilating bacterium
BR0203531A (pt) 2001-09-06 2003-09-09 Ajinomoto Kk Método para produzir uma substáncia alvo pelo uso de um microorganismo, e, microorganismo no qual um gene da metanol desidrogenase é introduzido
JP2004166595A (ja) 2002-11-20 2004-06-17 Ajinomoto Co Inc メチロトローフを用いたｌ−アミノ酸の製造法
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BR112012001351A2 (pt) 2009-07-22 2015-09-01 Univ California Método, célula de levedura engenheirada geneticamente, e, co-cultura de bactéria-levedura.

Also Published As

Publication number Publication date
CN104220591A (zh) 2014-12-17
US9322000B2 (en) 2016-04-26
KR102114507B1 (ko) 2020-05-25
US20150267177A1 (en) 2015-09-24
JP6185484B2 (ja) 2017-08-23
CA2862558A1 (en) 2013-08-01
KR20140131932A (ko) 2014-11-14
RU2014132203A (ru) 2016-03-20
GB201201178D0 (en) 2012-03-07
CN104220591B (zh) 2016-12-28
EP2807253A1 (en) 2014-12-03
RU2624017C2 (ru) 2017-06-30
WO2013110797A1 (en) 2013-08-01
EP2807253B1 (en) 2017-12-13
CA2862558C (en) 2021-07-06
JP2015504686A (ja) 2015-02-16

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