ES2563482T3 - Mejoras en nitrilasa microbiana inmovilizada para la producción de ácido glicólico - Google Patents
Mejoras en nitrilasa microbiana inmovilizada para la producción de ácido glicólico Download PDFInfo
- Publication number
- ES2563482T3 ES2563482T3 ES08843625.8T ES08843625T ES2563482T3 ES 2563482 T3 ES2563482 T3 ES 2563482T3 ES 08843625 T ES08843625 T ES 08843625T ES 2563482 T3 ES2563482 T3 ES 2563482T3
- Authority
- ES
- Spain
- Prior art keywords
- glutaraldehyde
- nitrilase
- immobilized
- catalyst
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 108010033272 Nitrilase Proteins 0.000 title abstract description 37
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 title abstract description 8
- 230000000813 microbial effect Effects 0.000 title description 3
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000003054 catalyst Substances 0.000 abstract description 23
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 18
- 125000003275 alpha amino acid group Chemical group 0.000 abstract description 15
- 108090000790 Enzymes Proteins 0.000 abstract description 10
- 102000004190 Enzymes Human genes 0.000 abstract description 10
- 229920001184 polypeptide Polymers 0.000 abstract description 9
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 9
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 9
- 230000002255 enzymatic effect Effects 0.000 abstract description 6
- 238000000855 fermentation Methods 0.000 abstract description 6
- 230000004151 fermentation Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- LTYRAPJYLUPLCI-UHFFFAOYSA-N glycolonitrile Chemical compound OCC#N LTYRAPJYLUPLCI-UHFFFAOYSA-N 0.000 abstract description 4
- JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 abstract description 4
- 230000014759 maintenance of location Effects 0.000 abstract description 4
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract description 3
- 229940113118 carrageenan Drugs 0.000 abstract 2
- 235000010418 carrageenan Nutrition 0.000 abstract 2
- 229920001525 carrageenan Polymers 0.000 abstract 2
- 239000000679 carrageenan Substances 0.000 abstract 2
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 abstract 2
- 229920002873 Polyethylenimine Polymers 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- 230000003100 immobilizing effect Effects 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 18
- 239000008103 glucose Substances 0.000 description 18
- 241000726118 Acidovorax facilis Species 0.000 description 16
- 238000006467 substitution reaction Methods 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 4
- 239000008101 lactose Substances 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 241000726119 Acidovorax Species 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- 241000488157 Escherichia sp. Species 0.000 description 2
- 241000589774 Pseudomonas sp. Species 0.000 description 2
- 241000316848 Rhodococcus <scale insect> Species 0.000 description 2
- 241000187693 Rhodococcus rhodochrous Species 0.000 description 2
- DWAQJAXMDSEUJJ-UHFFFAOYSA-M Sodium bisulfite Chemical compound [Na+].OS([O-])=O DWAQJAXMDSEUJJ-UHFFFAOYSA-M 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000010267 sodium hydrogen sulphite Nutrition 0.000 description 2
- 235000011149 sulphuric acid Nutrition 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000588813 Alcaligenes faecalis Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241000228257 Aspergillus sp. Species 0.000 description 1
- 241000099686 Azotobacter sp. Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- GUBGYTABKSRVRQ-DCSYEGIMSA-N Beta-Lactose Chemical compound OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-DCSYEGIMSA-N 0.000 description 1
- 241001406045 Bordetella bronchiseptica RB50 Species 0.000 description 1
- 241000589171 Bradyrhizobium sp. Species 0.000 description 1
- 241000186312 Brevibacterium sp. Species 0.000 description 1
- 241000873310 Citrobacter sp. Species 0.000 description 1
- 241000193464 Clostridium sp. Species 0.000 description 1
- 241001478312 Comamonas sp. Species 0.000 description 1
- 241000589518 Comamonas testosteroni Species 0.000 description 1
- 241000186249 Corynebacterium sp. Species 0.000 description 1
- 241001030162 Debaryomyces sp. Species 0.000 description 1
- 241001560459 Dunaliella sp. Species 0.000 description 1
- 241000147019 Enterobacter sp. Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000588754 Klebsiella sp. Species 0.000 description 1
- 241000170280 Kluyveromyces sp. Species 0.000 description 1
- 241000186610 Lactobacillus sp. Species 0.000 description 1
- 241001558145 Mucor sp. Species 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- 241001037736 Nocardia farcinica IFM 10152 Species 0.000 description 1
- 241000187681 Nocardia sp. Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 241000235061 Pichia sp. Species 0.000 description 1
- 241000514830 Pseudomonas entomophila L48 Species 0.000 description 1
- 241001358835 Pseudomonas fluorescens PF5 Species 0.000 description 1
- 241001209258 Pseudomonas syringae pv. syringae B728a Species 0.000 description 1
- 241000589187 Rhizobium sp. Species 0.000 description 1
- 241000187562 Rhodococcus sp. Species 0.000 description 1
- 241000235088 Saccharomyces sp. Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- 241000135402 Synechococcus elongatus PCC 6301 Species 0.000 description 1
- 241000192589 Synechococcus elongatus PCC 7942 Species 0.000 description 1
- 241000192560 Synechococcus sp. Species 0.000 description 1
- 241000192581 Synechocystis sp. Species 0.000 description 1
- 241000490645 Yarrowia sp. Species 0.000 description 1
- 241001030170 Zygosaccharomyces sp. Species 0.000 description 1
- 241000588901 Zymomonas Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 229940005347 alcaligenes faecalis Drugs 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000012840 feeding operation Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000001471 micro-filtration Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/42—Hydroxy-carboxylic acids
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/584—Recycling of catalysts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/930,550 US7695945B2 (en) | 2007-10-31 | 2007-10-31 | Immobilized microbial nitrilase for production of glycolic acid |
| US930550 | 2007-10-31 | ||
| PCT/US2008/081948 WO2009059101A1 (en) | 2007-10-31 | 2008-10-31 | Improvement in immobilized microbial nitrilase for production of glycolic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2563482T3 true ES2563482T3 (es) | 2016-03-15 |
Family
ID=40243876
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES08843625.8T Active ES2563482T3 (es) | 2007-10-31 | 2008-10-31 | Mejoras en nitrilasa microbiana inmovilizada para la producción de ácido glicólico |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7695945B2 (enExample) |
| EP (1) | EP2215225B1 (enExample) |
| JP (1) | JP5702604B2 (enExample) |
| CN (1) | CN101965399B (enExample) |
| DK (1) | DK2215225T3 (enExample) |
| ES (1) | ES2563482T3 (enExample) |
| PL (1) | PL2215225T3 (enExample) |
| WO (1) | WO2009059101A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012048865A2 (de) | 2010-10-12 | 2012-04-19 | C-Lecta Gmbh | Nitrilasen |
| GB2554708A (en) * | 2016-10-05 | 2018-04-11 | Chemoxy Int | Process for converting nitriles |
| CN111848495B (zh) * | 2020-06-02 | 2022-06-21 | 山东华素制药有限公司 | 一种1-苄基-3-哌啶醇的合成方法 |
| CN115845880B (zh) * | 2022-12-08 | 2024-06-14 | 复旦大学 | 一种水溶性高分子模板原位构筑过渡金属硫化物/碳复合材料及其制备方法和应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2175805A (en) * | 1937-06-08 | 1939-10-10 | Du Pont | Stabilized organic nitrile and process of making |
| US2890238A (en) * | 1957-01-31 | 1959-06-09 | Dow Chemical Co | Preparation of glyconitrile |
| GB1553662A (en) | 1978-04-13 | 1979-09-26 | Union Carbide Corp | Sterilization of fermentation media |
| DK146942C (da) * | 1978-04-19 | 1984-07-30 | Novo Industri As | Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt |
| US4355105A (en) * | 1981-03-30 | 1982-10-19 | Miles Laboratories, Inc. | Glutaraldehyde/polyethylenimine immobilization of whole microbial cells |
| US5187301A (en) * | 1989-10-26 | 1993-02-16 | W. R. Grace & Co.-Conn. | Preparation of iminodiacetonitrile from glycolonitrile |
| DE69126762T2 (de) * | 1990-11-14 | 1997-10-23 | Nitto Chemical Industry Co Ltd | Biologisches Verfahren zur Herstellung von Alpha-Hydroxyamid und Alpha-Hydroxysäure |
| JP3354688B2 (ja) * | 1994-01-28 | 2002-12-09 | 三菱レイヨン株式会社 | 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法 |
| US6037155A (en) * | 1997-02-27 | 2000-03-14 | Nippon Soda Co., Ltd. | Process for preparing α-hydroxy acids using microorganism and novel microorganism |
| US6251646B1 (en) * | 1999-07-12 | 2001-06-26 | E. I. Du Pont De Nemours And Company | Method for stabilizing nitrilase activity and preserving microbial cells |
| US6551804B2 (en) * | 1999-07-12 | 2003-04-22 | E. I. Du Pont De Nemours And Company | Process for preparing 4-cyanopentanoic acid |
| KR100501864B1 (ko) * | 1999-11-12 | 2005-07-20 | 미츠비시 레이온 가부시키가이샤 | 사멸화 균체 |
| ATE495244T1 (de) * | 2000-03-31 | 2011-01-15 | Du Pont | Isolierung und expression eines nitrilase-gens von acidovorax facilis 72w |
| US6416980B1 (en) * | 2001-02-23 | 2002-07-09 | E. I. Du Pont De Nemours & Company | Method for producing glycolic acid from glycolonitrile using nitrilase |
| KR100869623B1 (ko) | 2004-04-27 | 2008-11-21 | 미쓰이 가가쿠 가부시키가이샤 | 하이드록시 카복실산류의 생산 방법 |
| US7148051B2 (en) * | 2004-08-16 | 2006-12-12 | E. I. Du Pont De Nemours And Company | Production of 3-hydroxycarboxylic acid using nitrilase |
| EP1828393B1 (en) | 2004-12-22 | 2012-11-07 | E.I. Du Pont De Nemours And Company | Process for producing glycolic acid from formaldehyde and hydrogen cyanide |
| US7198927B2 (en) * | 2004-12-22 | 2007-04-03 | E. I. Du Pont De Nemours And Company | Enzymatic production of glycolic acid |
| WO2006071663A1 (en) * | 2004-12-22 | 2006-07-06 | E.I. Dupont De Nemours And Company | Process for the synthesis of glycolonitrile |
| US7445917B2 (en) * | 2004-12-22 | 2008-11-04 | E.I. Du Pont De Nemours And Company | Process for producing glycolic acid from formaldehyde and hydrogen cyanide |
| CN101278047B (zh) * | 2005-09-30 | 2012-12-12 | 诺维信公司 | 酶的固定化 |
-
2007
- 2007-10-31 US US11/930,550 patent/US7695945B2/en active Active
-
2008
- 2008-10-31 JP JP2010532267A patent/JP5702604B2/ja not_active Expired - Fee Related
- 2008-10-31 CN CN200880124121.XA patent/CN101965399B/zh not_active Expired - Fee Related
- 2008-10-31 EP EP08843625.8A patent/EP2215225B1/en active Active
- 2008-10-31 WO PCT/US2008/081948 patent/WO2009059101A1/en not_active Ceased
- 2008-10-31 DK DK08843625.8T patent/DK2215225T3/en active
- 2008-10-31 ES ES08843625.8T patent/ES2563482T3/es active Active
- 2008-10-31 PL PL08843625T patent/PL2215225T3/pl unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20090111158A1 (en) | 2009-04-30 |
| US7695945B2 (en) | 2010-04-13 |
| CN101965399B (zh) | 2016-06-08 |
| PL2215225T3 (pl) | 2016-05-31 |
| EP2215225B1 (en) | 2015-12-02 |
| JP5702604B2 (ja) | 2015-04-15 |
| JP2011501969A (ja) | 2011-01-20 |
| WO2009059101A1 (en) | 2009-05-07 |
| EP2215225A1 (en) | 2010-08-11 |
| CN101965399A (zh) | 2011-02-02 |
| DK2215225T3 (en) | 2016-03-07 |
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