ES2532641T3 - Marcadores para su utilización en la producción de líneas restauradoras doble cero de Brassica napus con un buen valor agronómico - Google Patents
Marcadores para su utilización en la producción de líneas restauradoras doble cero de Brassica napus con un buen valor agronómico Download PDFInfo
- Publication number
- ES2532641T3 ES2532641T3 ES10183322.6T ES10183322T ES2532641T3 ES 2532641 T3 ES2532641 T3 ES 2532641T3 ES 10183322 T ES10183322 T ES 10183322T ES 2532641 T3 ES2532641 T3 ES 2532641T3
- Authority
- ES
- Spain
- Prior art keywords
- markers
- production
- marker
- brassica napus
- good agronomic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 240000002791 Brassica napus Species 0.000 title description 2
- 235000011293 Brassica napus Nutrition 0.000 title description 2
- 238000004519 manufacturing process Methods 0.000 title description 2
- 230000009418 agronomic effect Effects 0.000 title 1
- 239000003550 marker Substances 0.000 abstract description 14
- 239000000203 mixture Substances 0.000 abstract description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 240000007124 Brassica oleracea Species 0.000 description 2
- 240000008100 Brassica rapa Species 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 101000733590 Streptomyces griseus subsp. griseus (strain JCM 4626 / NBRC 13350) Aminopeptidase S Proteins 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 235000011303 Brassica alboglabra Nutrition 0.000 description 1
- 235000011302 Brassica oleracea Nutrition 0.000 description 1
- 235000011292 Brassica rapa Nutrition 0.000 description 1
- 101100276976 Drosophila melanogaster Drak gene Proteins 0.000 description 1
- 101710129448 Glucose-6-phosphate isomerase 2 Proteins 0.000 description 1
- 101100067573 Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) pgi2 gene Proteins 0.000 description 1
- 235000000245 Raphanus raphanistrum subsp raphanistrum Nutrition 0.000 description 1
- 235000011380 Raphanus sativus Nutrition 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000005251 gamma ray Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
- A01H6/202—Brassica napus [canola]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Microbiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Composición de cinco marcadores, siendo dichos marcadores PGlol, PGIUNT, PGlint, BolJon y CP418, en la que el marcador PGlol presenta la secuencia de SEC ID nº: 1, el marcador PGIUNT presenta la secuencia de SEC ID nº: 2, el marcador PGlint presenta la secuencia de SEC ID nº: 3, el marcador BolJon presenta la secuencia de SEC ID nº: 4, y el marcador CP418 presenta la secuencia de SEC ID nº:5.
Description
E10183322
11-03-2015
marcador PGIol R2000:
marcador PGIUNT R2000:
marcador PGIint R2000:
marcador BoIJon R2000:
marcador CP418L R2000:
4 E10183322
11-03-2015
En el dibujo adjunto a continuación, se utilizan las siguientes abreviaturas:
- Dra.
- Drakkar
- Rel-15-1, E38, R15
- R2000
- Hete, Hel, R211.Drakkar
- R211 heterocigótico* Drakkar
- Darm
- Darmor
- Bol:
- Brassica oleracea
- Bra, B.rap:
- Brassica rapa
- GCPA18-A19, Wes, Aust:
- Wesroona
- Sam, SamlPGIolSunt5
- Samourai
- RRH1, ba2c
- RRH1
- rav, N.WR
- Brassica napus híbrida * Rábano silvestre
- 5
- - La figura 1 ilustra la irradiación con rayos gamma y la producción de F2.
- -
- La figura 2 ilustra semillas colocadas en 'R211' y 'R2000'.
- 10
- - La figura 3 muestra el número de semillas por vaina de diferentes líneas.
- -
- La figura 4 ilustra la localización del cebador PGIol en el segmento de la secuencia de PGI de la base de datos. En esa figura:
- 15
- PGIol: -cebador PGIol U (nombrado en SGAP: BnPGIch 1 U) -cebador PGIol L (nombrado en SGAP: Bn PGIch 1 L)
- 20
- - PGIint: -cebador PGIint U -cebador PGIint L (está fuera de la secuencia). La figura 5 ilustra la electroforesis en gel del gen PGI-2 (PGIol), marcador de RCP y SG34, marcador de RCP cerca de Rfo.
- 25
- -- La figura 6 ilustra el segmento Pgi-2 de ADN ampliado por RCP con cebadores PGIol. La figura 7 ilustra la digestión del producto de RCP PGIol por MseI.
- En esa figura:
- 30
- Sam y Darm tienen una banda de 75 pb. Drak, R211.Dk y R2000 mostró una banda de 70pb (acrilamida al 15%). 8 fue similar a Samourai (75 pb); la mezcla con Drakkar (70 pb) permitió la visualización de las dos bandas.
- 35
- - La figura 8 ilustra la electroforesis en gel de agarosa del marcador PGIUNT. En esa figura:
- 40
- La banda PGIUNT (alrededor de 980 pb) está presente en B. oleracea, B. rapa cv (siglas en inglés de: variedad cultivada) Asko, líneas mantenedoras y restauradas, excepto en 'R211'. No existe ampliación en el rábano ni en Arabidopsis.
- 45
- - En varios genotipos de Brassica sólo se amplió una banda. Los tamaños de las bandas son similares, pero las secuencias son diferentes. La figura 9 ilustra la electroforesis en gel del marcador PGIint de RCP.
5
Claims (1)
-
imagen1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03291677 | 2003-07-04 | ||
| EP03291677 | 2003-07-04 | ||
| EP03293057A EP1493328A1 (en) | 2003-07-04 | 2003-12-08 | Method of producing double low restorer lines of brassica napus having a good agronomic value |
| EP03293057 | 2003-12-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2532641T3 true ES2532641T3 (es) | 2015-03-30 |
Family
ID=33436133
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09175998T Expired - Lifetime ES2394990T3 (es) | 2003-07-04 | 2004-07-05 | Procedimiento de producción de líneas restauradoras doble cero de Brassica napus con un buen valor agronómico |
| ES10183322.6T Expired - Lifetime ES2532641T3 (es) | 2003-07-04 | 2004-07-05 | Marcadores para su utilización en la producción de líneas restauradoras doble cero de Brassica napus con un buen valor agronómico |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09175998T Expired - Lifetime ES2394990T3 (es) | 2003-07-04 | 2004-07-05 | Procedimiento de producción de líneas restauradoras doble cero de Brassica napus con un buen valor agronómico |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7812217B2 (es) |
| EP (4) | EP1493328A1 (es) |
| CN (1) | CN1816276B (es) |
| AU (2) | AU2004253363A1 (es) |
| CA (1) | CA2531051C (es) |
| CY (1) | CY1116159T1 (es) |
| DK (2) | DK2179643T3 (es) |
| ES (2) | ES2394990T3 (es) |
| HK (1) | HK1094409A1 (es) |
| PL (2) | PL2179643T3 (es) |
| PT (2) | PT2179643E (es) |
| RU (1) | RU2373698C2 (es) |
| SI (2) | SI2298066T1 (es) |
| WO (1) | WO2005002324A2 (es) |
Families Citing this family (190)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CL2007003743A1 (es) | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | Composicion que comprende fenamidona y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos. |
| CL2007003744A1 (es) | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | Composicion que comprende un derivado 2-piridilmetilbenzamida y un compuesto insecticida; y metodo para controlar de forma curativa o preventiva hongos fitopatogenos de cultivos e insectos. |
| EP1969930A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Phenoxyphenylamidine und deren Verwendung als Fungizide |
| EP2136627B1 (de) | 2007-03-12 | 2015-05-13 | Bayer Intellectual Property GmbH | Dihalogenphenoxyphenylamidine und deren verwendung als fungizide |
| WO2008110281A2 (de) | 2007-03-12 | 2008-09-18 | Bayer Cropscience Ag | 3,4-disubstituierte phenoxyphenylamidine und deren verwendung als fungizide |
| EP1969931A1 (de) * | 2007-03-12 | 2008-09-17 | Bayer CropScience Aktiengesellschaft | Fluoalkylphenylamidine und deren Verwendung als Fungizide |
| EP1969929A1 (de) | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Substituierte Phenylamidine und deren Verwendung als Fungizide |
| EP1969934A1 (de) * | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | 4-Cycloalkyl-oder 4-arylsubstituierte Phenoxyphenylamidine und deren Verwendung als Fungizide |
| JP2010524869A (ja) | 2007-04-19 | 2010-07-22 | バイエル・クロツプサイエンス・アクチエンゲゼルシヤフト | チアジアゾリルオキシフェニルアミジンおよび殺菌剤としてのこれらの使用 |
| EP2016821A1 (en) | 2007-06-13 | 2009-01-21 | Syngeta Participations AG | New hybrid system for Brassica napus |
| DE102007045956A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombination mit insektiziden und akariziden Eigenschaften |
| DE102007045919B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
| DE102007045957A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akarziden Eigenschaften |
| DE102007045955A1 (de) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
| DE102007045953B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
| DE102007045920B4 (de) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Synergistische Wirkstoffkombinationen |
| DE102007045922A1 (de) | 2007-09-26 | 2009-04-02 | Bayer Cropscience Ag | Wirkstoffkombinationen mit insektiziden und akariziden Eigenschaften |
| EP2090168A1 (de) | 2008-02-12 | 2009-08-19 | Bayer CropScience AG | Methode zur Verbesserung des Pflanzenwachstums |
| EP2072506A1 (de) | 2007-12-21 | 2009-06-24 | Bayer CropScience AG | Thiazolyloxyphenylamidine oder Thiadiazolyloxyphenylamidine und deren Verwendung als Fungizide |
| US8466347B2 (en) | 2008-02-06 | 2013-06-18 | Pioneer Hi Bred International Inc | Brassica ogura restorer lines with shortened raphanus fragment (SRF) |
| EP2168434A1 (de) | 2008-08-02 | 2010-03-31 | Bayer CropScience AG | Verwendung von Azolen zur Steigerung der Resistenz von Pflanzen oder Pflanzenteilen gegenüber abiotischem Stress |
| US20110190365A1 (en) | 2008-08-14 | 2011-08-04 | Bayer Crop Science Ag | Insecticidal 4-phenyl-1H-pyrazoles |
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| JP2012521371A (ja) | 2009-03-25 | 2012-09-13 | バイエル・クロップサイエンス・アーゲー | 殺虫特性および殺ダニ特性を有する活性化合物の組合せ |
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| WO2011015524A2 (en) | 2009-08-03 | 2011-02-10 | Bayer Cropscience Ag | Fungicide heterocycles derivatives |
| FR2948533A1 (fr) * | 2009-08-03 | 2011-02-04 | Limagrain Verneuil Holding | Plante brassica restauratrice de la sterilite male cytoplasmique ogura, procede de production et utilisation de cette plante |
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| WO2018104392A1 (en) | 2016-12-08 | 2018-06-14 | Bayer Cropscience Aktiengesellschaft | Use of insecticides for controlling wireworms |
| EP3332645A1 (de) | 2016-12-12 | 2018-06-13 | Bayer Cropscience AG | Verwendung substituierter pyrimidindione oder jeweils deren salze als wirkstoffe gegen abiotischen pflanzenstress |
| WO2018108627A1 (de) | 2016-12-12 | 2018-06-21 | Bayer Cropscience Aktiengesellschaft | Verwendung substituierter indolinylmethylsulfonamide oder deren salze zur steigerung der stresstoleranz in pflanzen |
| CN106967803B (zh) * | 2017-03-21 | 2020-09-29 | 北京市农林科学院 | 一种检测萝卜Ogura-CMS育性恢复基因的高通量分子标记及应用 |
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| CN107593426B (zh) * | 2017-09-27 | 2019-12-10 | 上海市农业科学院 | 一种甘蓝型油菜显性核不育恢复系的选育方法 |
| CN112513033A (zh) | 2018-06-04 | 2021-03-16 | 拜耳公司 | 除草活性的双环苯甲酰基吡唑 |
| CN112689457A (zh) | 2018-07-26 | 2021-04-20 | 拜耳公司 | 琥珀酸脱氢酶抑制剂氟吡菌酰胺用于防治十字花科物种中由立枯丝核菌、镰刀菌属种和腐霉菌属种引起的根腐病复合症和/或苗期病害复合症的用途 |
| BR112021004865A2 (pt) | 2018-09-17 | 2021-06-01 | Bayer Aktiengesellschaft | uso do fungicida isoflucypram para controlar claviceps purpurea e reduzir esclerócios em cereais |
| US20220039383A1 (en) | 2018-09-17 | 2022-02-10 | Bayer Aktiengesellschaft | Use of the Succinate Dehydrogenase Inhibitor Fluopyram for Controlling Claviceps Purpurea and Reducing Sclerotia in Cereals |
| CN112259164B (zh) * | 2020-10-28 | 2022-07-05 | 湖南省作物研究所 | 一种萝卜细胞质不育恢复系共分离的竞争性indel分子标记的开发方法 |
| CA3201992A1 (en) | 2020-12-21 | 2022-06-30 | Thi Ninh Thuan NGUYEN | Brassica napus plants comprising an improved fertility restorer |
| CN113355453B (zh) * | 2021-08-09 | 2021-10-29 | 华智生物技术有限公司 | 一种甘蓝型油菜萝卜细胞质不育恢复基因Rfo的SNP分子标记及其应用 |
| CN116042889B (zh) * | 2022-09-29 | 2023-08-29 | 安徽科技学院 | 一种与甘蓝型油菜开花期主效qtl位点连锁的分子标记及其应用 |
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| CA2193938A1 (en) * | 1996-12-24 | 1998-06-24 | David G. Charne | Oilseed brassica containing an improved fertility restorer gene for ogura cytoplasmic male sterility |
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