ES2530203T3 - Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución - Google Patents
Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución Download PDFInfo
- Publication number
- ES2530203T3 ES2530203T3 ES07751131.9T ES07751131T ES2530203T3 ES 2530203 T3 ES2530203 T3 ES 2530203T3 ES 07751131 T ES07751131 T ES 07751131T ES 2530203 T3 ES2530203 T3 ES 2530203T3
- Authority
- ES
- Spain
- Prior art keywords
- matrix
- under study
- analyte
- sites
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012491 analyte Substances 0.000 title claims abstract description 219
- 238000000034 method Methods 0.000 title claims abstract description 140
- 238000001514 detection method Methods 0.000 title description 55
- 239000011159 matrix material Substances 0.000 claims abstract description 268
- 102000004190 Enzymes Human genes 0.000 claims description 169
- 108090000790 Enzymes Proteins 0.000 claims description 169
- 239000000835 fiber Substances 0.000 claims description 83
- 230000003287 optical effect Effects 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 33
- 102000004169 proteins and genes Human genes 0.000 claims description 33
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 150000007523 nucleic acids Chemical class 0.000 claims description 26
- 102000005962 receptors Human genes 0.000 claims description 25
- 108020003175 receptors Proteins 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 25
- 230000008859 change Effects 0.000 claims description 16
- 150000001720 carbohydrates Chemical class 0.000 claims description 8
- 235000014633 carbohydrates Nutrition 0.000 claims description 8
- 239000000427 antigen Substances 0.000 claims description 6
- 108091007433 antigens Proteins 0.000 claims description 6
- 102000036639 antigens Human genes 0.000 claims description 6
- 229940088597 hormone Drugs 0.000 claims description 6
- 239000005556 hormone Substances 0.000 claims description 6
- 102000004127 Cytokines Human genes 0.000 claims description 5
- 108090000695 Cytokines Proteins 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 230000007613 environmental effect Effects 0.000 claims description 4
- 230000003054 hormonal effect Effects 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 4
- 230000001537 neural effect Effects 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 239000000575 pesticide Substances 0.000 claims description 4
- 239000003053 toxin Substances 0.000 claims description 4
- 231100000765 toxin Toxicity 0.000 claims description 4
- 108700012359 toxins Proteins 0.000 claims description 4
- 230000001413 cellular effect Effects 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 claims description 3
- 239000002917 insecticide Substances 0.000 claims description 3
- 230000027455 binding Effects 0.000 description 120
- 239000000523 sample Substances 0.000 description 108
- 239000000758 substrate Substances 0.000 description 107
- 238000006243 chemical reaction Methods 0.000 description 101
- 210000004027 cell Anatomy 0.000 description 84
- 239000000243 solution Substances 0.000 description 65
- 239000003446 ligand Substances 0.000 description 61
- 239000003112 inhibitor Substances 0.000 description 59
- 239000002105 nanoparticle Substances 0.000 description 44
- 230000002255 enzymatic effect Effects 0.000 description 42
- 239000000412 dendrimer Substances 0.000 description 37
- 229920000736 dendritic polymer Polymers 0.000 description 37
- 239000000463 material Substances 0.000 description 30
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 28
- 239000010931 gold Substances 0.000 description 28
- 229910052737 gold Inorganic materials 0.000 description 27
- 238000003556 assay Methods 0.000 description 26
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 25
- 229920000642 polymer Polymers 0.000 description 25
- 239000002245 particle Substances 0.000 description 24
- 108020004414 DNA Proteins 0.000 description 23
- 238000009826 distribution Methods 0.000 description 23
- 239000000203 mixture Substances 0.000 description 23
- 239000000047 product Substances 0.000 description 23
- 238000002474 experimental method Methods 0.000 description 18
- 238000011002 quantification Methods 0.000 description 16
- 239000000126 substance Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 16
- 239000012470 diluted sample Substances 0.000 description 14
- 229920001296 polysiloxane Polymers 0.000 description 14
- JCLFHZLOKITRCE-UHFFFAOYSA-N 4-pentoxyphenol Chemical compound CCCCCOC1=CC=C(O)C=C1 JCLFHZLOKITRCE-UHFFFAOYSA-N 0.000 description 13
- 229960002685 biotin Drugs 0.000 description 13
- 235000020958 biotin Nutrition 0.000 description 13
- 239000011616 biotin Substances 0.000 description 13
- 238000004519 manufacturing process Methods 0.000 description 13
- 230000004048 modification Effects 0.000 description 13
- 238000012986 modification Methods 0.000 description 13
- 239000013307 optical fiber Substances 0.000 description 13
- 125000000524 functional group Chemical group 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 11
- 239000011248 coating agent Substances 0.000 description 11
- 238000000576 coating method Methods 0.000 description 11
- 239000004065 semiconductor Substances 0.000 description 11
- 239000004971 Cross linker Substances 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 239000002096 quantum dot Substances 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 9
- 239000011521 glass Substances 0.000 description 9
- 239000002159 nanocrystal Substances 0.000 description 9
- -1 polypropylene Polymers 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 108020004999 messenger RNA Proteins 0.000 description 8
- 239000004054 semiconductor nanocrystal Substances 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 125000003277 amino group Chemical group 0.000 description 7
- 108010090804 Streptavidin Proteins 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 238000006911 enzymatic reaction Methods 0.000 description 6
- 230000005284 excitation Effects 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 239000012062 aqueous buffer Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- UHYPYGJEEGLRJD-UHFFFAOYSA-N cadmium(2+);selenium(2-) Chemical compound [Se-2].[Cd+2] UHYPYGJEEGLRJD-UHFFFAOYSA-N 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000007865 diluting Methods 0.000 description 5
- 150000003573 thiols Chemical group 0.000 description 5
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 125000000217 alkyl group Chemical group 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 102000005936 beta-Galactosidase Human genes 0.000 description 4
- 108010005774 beta-Galactosidase Proteins 0.000 description 4
- 230000003197 catalytic effect Effects 0.000 description 4
- 238000006555 catalytic reaction Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000003593 chromogenic compound Substances 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 4
- 239000002532 enzyme inhibitor Substances 0.000 description 4
- 229940125532 enzyme inhibitor Drugs 0.000 description 4
- 230000014509 gene expression Effects 0.000 description 4
- 238000003384 imaging method Methods 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000009871 nonspecific binding Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- HSSLDCABUXLXKM-UHFFFAOYSA-N resorufin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3N=C21 HSSLDCABUXLXKM-UHFFFAOYSA-N 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 238000002444 silanisation Methods 0.000 description 4
- 239000000377 silicon dioxide Substances 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000010998 test method Methods 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- YMXHPSHLTSZXKH-RVBZMBCESA-N (2,5-dioxopyrrolidin-1-yl) 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoate Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)ON1C(=O)CCC1=O YMXHPSHLTSZXKH-RVBZMBCESA-N 0.000 description 3
- ZPZDIFSPRVHGIF-UHFFFAOYSA-N 3-aminopropylsilicon Chemical compound NCCC[Si] ZPZDIFSPRVHGIF-UHFFFAOYSA-N 0.000 description 3
- 239000012109 Alexa Fluor 568 Substances 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 102100037362 Fibronectin Human genes 0.000 description 3
- 108010067306 Fibronectins Proteins 0.000 description 3
- 229910004042 HAuCl4 Inorganic materials 0.000 description 3
- 108010001267 Protein Subunits Proteins 0.000 description 3
- 102000002067 Protein Subunits Human genes 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000002902 bimodal effect Effects 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 239000011247 coating layer Substances 0.000 description 3
- 239000000084 colloidal system Substances 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 230000009089 cytolysis Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 108010037896 heparin-binding hemagglutinin Proteins 0.000 description 3
- 229940127121 immunoconjugate Drugs 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000005304 joining Methods 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 102000044158 nucleic acid binding protein Human genes 0.000 description 3
- 108700020942 nucleic acid binding protein Proteins 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 238000005498 polishing Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 210000001236 prokaryotic cell Anatomy 0.000 description 3
- 229920002379 silicone rubber Polymers 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 230000007306 turnover Effects 0.000 description 3
- 102000005600 Cathepsins Human genes 0.000 description 2
- 108010084457 Cathepsins Proteins 0.000 description 2
- 229910004613 CdTe Inorganic materials 0.000 description 2
- 102000000844 Cell Surface Receptors Human genes 0.000 description 2
- 108010001857 Cell Surface Receptors Proteins 0.000 description 2
- YVECGMZCTULTIS-HSUXUTPPSA-N D-galactal Chemical compound OC[C@H]1OC=C[C@@H](O)[C@H]1O YVECGMZCTULTIS-HSUXUTPPSA-N 0.000 description 2
- 102000001301 EGF receptor Human genes 0.000 description 2
- 108060006698 EGF receptor Proteins 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 2
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 2
- 101001024703 Homo sapiens Nck-associated protein 5 Proteins 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- 229910000673 Indium arsenide Inorganic materials 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- 102000004856 Lectins Human genes 0.000 description 2
- PEEHTFAAVSWFBL-UHFFFAOYSA-N Maleimide Chemical compound O=C1NC(=O)C=C1 PEEHTFAAVSWFBL-UHFFFAOYSA-N 0.000 description 2
- 102000018697 Membrane Proteins Human genes 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 102100036946 Nck-associated protein 5 Human genes 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 229910007709 ZnTe Inorganic materials 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229920006397 acrylic thermoplastic Polymers 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 238000000089 atomic force micrograph Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000010511 deprotection reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000001493 electron microscopy Methods 0.000 description 2
- 238000001952 enzyme assay Methods 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 229910052732 germanium Inorganic materials 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- RPQDHPTXJYYUPQ-UHFFFAOYSA-N indium arsenide Chemical compound [In]#[As] RPQDHPTXJYYUPQ-UHFFFAOYSA-N 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000005291 magnetic effect Effects 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- NHBRUUFBSBSTHM-UHFFFAOYSA-N n'-[2-(3-trimethoxysilylpropylamino)ethyl]ethane-1,2-diamine Chemical compound CO[Si](OC)(OC)CCCNCCNCCN NHBRUUFBSBSTHM-UHFFFAOYSA-N 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- ISXSCDLOGDJUNJ-UHFFFAOYSA-N tert-butyl prop-2-enoate Chemical compound CC(C)(C)OC(=O)C=C ISXSCDLOGDJUNJ-UHFFFAOYSA-N 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- PMJWDPGOWBRILU-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-[4-(2,5-dioxopyrrol-1-yl)phenyl]butanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCCC(C=C1)=CC=C1N1C(=O)C=CC1=O PMJWDPGOWBRILU-UHFFFAOYSA-N 0.000 description 1
- YBNMDCCMCLUHBL-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 4-pyren-1-ylbutanoate Chemical compound C=1C=C(C2=C34)C=CC3=CC=CC4=CC=C2C=1CCCC(=O)ON1C(=O)CCC1=O YBNMDCCMCLUHBL-UHFFFAOYSA-N 0.000 description 1
- DYMYLBQTHCJHOQ-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) butanoate Chemical compound CCCC(=O)ON1C(=O)CCC1=O DYMYLBQTHCJHOQ-UHFFFAOYSA-N 0.000 description 1
- MIAKOEWBCMPCQR-YBXAARCKSA-N (2s,3r,4s,5r,6r)-2-(4-aminophenoxy)-6-(hydroxymethyl)oxane-3,4,5-triol Chemical group C1=CC(N)=CC=C1O[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MIAKOEWBCMPCQR-YBXAARCKSA-N 0.000 description 1
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 1
- VHYFNPMBLIVWCW-UHFFFAOYSA-N 4-Dimethylaminopyridine Chemical compound CN(C)C1=CC=NC=C1 VHYFNPMBLIVWCW-UHFFFAOYSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 108091023037 Aptamer Proteins 0.000 description 1
- 229910015808 BaTe Inorganic materials 0.000 description 1
- 229910004813 CaTe Inorganic materials 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000011727 Caspases Human genes 0.000 description 1
- 108010076667 Caspases Proteins 0.000 description 1
- 240000006670 Chlorogalum pomeridianum Species 0.000 description 1
- 235000007836 Chlorogalum pomeridianum Nutrition 0.000 description 1
- 102000016989 Ciliary Neurotrophic Factor Receptor Human genes 0.000 description 1
- 108010000063 Ciliary Neurotrophic Factor Receptor Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 101710095468 Cyclase Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000008836 DNA modification Effects 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108010092408 Eosinophil Peroxidase Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 239000004606 Fillers/Extenders Substances 0.000 description 1
- 229910002601 GaN Inorganic materials 0.000 description 1
- 229910005540 GaP Inorganic materials 0.000 description 1
- 229910005542 GaSb Inorganic materials 0.000 description 1
- 102000058061 Glucose Transporter Type 4 Human genes 0.000 description 1
- 108091052347 Glucose transporter family Proteins 0.000 description 1
- 102000042092 Glucose transporter family Human genes 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102100020948 Growth hormone receptor Human genes 0.000 description 1
- 208000010496 Heart Arrest Diseases 0.000 description 1
- 229910004262 HgTe Inorganic materials 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- GPXJNWSHGFTCBW-UHFFFAOYSA-N Indium phosphide Chemical compound [In]#P GPXJNWSHGFTCBW-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 102000003815 Interleukin-11 Human genes 0.000 description 1
- 108090000177 Interleukin-11 Proteins 0.000 description 1
- 102000013462 Interleukin-12 Human genes 0.000 description 1
- 108010065805 Interleukin-12 Proteins 0.000 description 1
- 102000003816 Interleukin-13 Human genes 0.000 description 1
- 108090000176 Interleukin-13 Proteins 0.000 description 1
- 102000003812 Interleukin-15 Human genes 0.000 description 1
- 108090000172 Interleukin-15 Proteins 0.000 description 1
- 108050003558 Interleukin-17 Proteins 0.000 description 1
- 102000013691 Interleukin-17 Human genes 0.000 description 1
- 108010002350 Interleukin-2 Proteins 0.000 description 1
- 108090000978 Interleukin-4 Proteins 0.000 description 1
- 102000004388 Interleukin-4 Human genes 0.000 description 1
- 108010002616 Interleukin-5 Proteins 0.000 description 1
- 102000000743 Interleukin-5 Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 102000004889 Interleukin-6 Human genes 0.000 description 1
- 108010002586 Interleukin-7 Proteins 0.000 description 1
- 102000000704 Interleukin-7 Human genes 0.000 description 1
- 108090001007 Interleukin-8 Proteins 0.000 description 1
- 102000004890 Interleukin-8 Human genes 0.000 description 1
- 108010002335 Interleukin-9 Proteins 0.000 description 1
- 102000000585 Interleukin-9 Human genes 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 102000004195 Isomerases Human genes 0.000 description 1
- 108090000769 Isomerases Proteins 0.000 description 1
- 108010001831 LDL receptors Proteins 0.000 description 1
- 102000000853 LDL receptors Human genes 0.000 description 1
- 241000713666 Lentivirus Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102100039364 Metalloproteinase inhibitor 1 Human genes 0.000 description 1
- 229910017680 MgTe Inorganic materials 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108091008606 PDGF receptors Proteins 0.000 description 1
- 102000011653 Platelet-Derived Growth Factor Receptors Human genes 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108010002519 Prolactin Receptors Proteins 0.000 description 1
- 102100029000 Prolactin receptor Human genes 0.000 description 1
- 108091006300 SLC2A4 Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 108010068542 Somatotropin Receptors Proteins 0.000 description 1
- 229910004411 SrTe Inorganic materials 0.000 description 1
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Natural products C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 102100027188 Thyroid peroxidase Human genes 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108091008605 VEGF receptors Proteins 0.000 description 1
- 102000009484 Vascular Endothelial Growth Factor Receptors Human genes 0.000 description 1
- 239000005083 Zinc sulfide Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- GZCGUPFRVQAUEE-KCDKBNATSA-N aldehydo-D-galactose Chemical class OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-KCDKBNATSA-N 0.000 description 1
- 150000001356 alkyl thiols Chemical class 0.000 description 1
- 230000008850 allosteric inhibition Effects 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 230000006287 biotinylation Effects 0.000 description 1
- 229920001400 block copolymer Polymers 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000001818 capillary gel electrophoresis Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 125000002843 carboxylic acid group Chemical group 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000002458 cell surface marker Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007398 colorimetric assay Methods 0.000 description 1
- 238000012875 competitive assay Methods 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000002330 electrospray ionisation mass spectrometry Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002270 exclusion chromatography Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- ZBKIUFWVEIBQRT-UHFFFAOYSA-N gold(1+) Chemical compound [Au+] ZBKIUFWVEIBQRT-UHFFFAOYSA-N 0.000 description 1
- 108010064060 high density lipoprotein receptors Proteins 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 108091008039 hormone receptors Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 238000013095 identification testing Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012216 imaging agent Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- WPYVAWXEWQSOGY-UHFFFAOYSA-N indium antimonide Chemical compound [Sb]#[In] WPYVAWXEWQSOGY-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 229910021432 inorganic complex Inorganic materials 0.000 description 1
- 229940047124 interferons Drugs 0.000 description 1
- 102000002467 interleukin receptors Human genes 0.000 description 1
- 108010093036 interleukin receptors Proteins 0.000 description 1
- 229940047122 interleukins Drugs 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 102000005861 leptin receptors Human genes 0.000 description 1
- 108010019813 leptin receptors Proteins 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002122 magnetic nanoparticle Substances 0.000 description 1
- 125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000000051 modifying effect Effects 0.000 description 1
- 230000009149 molecular binding Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000000569 multi-angle light scattering Methods 0.000 description 1
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 108091008104 nucleic acid aptamers Proteins 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 125000004043 oxo group Chemical group O=* 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000011049 pearl Substances 0.000 description 1
- 230000001175 peptic effect Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229940127557 pharmaceutical product Drugs 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001748 polybutylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 101150106084 queG gene Proteins 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 235000020095 red wine Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- SBIBMFFZSBJNJF-UHFFFAOYSA-N selenium;zinc Chemical compound [Se]=[Zn] SBIBMFFZSBJNJF-UHFFFAOYSA-N 0.000 description 1
- 238000001338 self-assembly Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 150000003376 silicon Chemical class 0.000 description 1
- ADZWSOLPGZMUMY-UHFFFAOYSA-M silver bromide Chemical compound [Ag]Br ADZWSOLPGZMUMY-UHFFFAOYSA-M 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 125000000547 substituted alkyl group Chemical group 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- 125000001174 sulfone group Chemical group 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- DRDVZXDWVBGGMH-UHFFFAOYSA-N zinc;sulfide Chemical compound [S-2].[Zn+2] DRDVZXDWVBGGMH-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Nanotechnology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Chemical Kinetics & Catalysis (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US77569206P | 2006-02-21 | 2006-02-21 | |
| US775692P | 2006-02-21 | ||
| US79273606P | 2006-04-17 | 2006-04-17 | |
| US792736P | 2006-04-17 | ||
| PCT/US2007/004349 WO2007098148A2 (en) | 2006-02-21 | 2007-02-20 | Mthods and arrays for target analyte detection and determination of target analyte concentration in solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2530203T3 true ES2530203T3 (es) | 2015-02-27 |
Family
ID=38437946
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07751131.9T Active ES2530203T3 (es) | 2006-02-21 | 2007-02-20 | Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución |
| ES12177276.8T Active ES2613083T3 (es) | 2006-02-21 | 2007-02-20 | Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES12177276.8T Active ES2613083T3 (es) | 2006-02-21 | 2007-02-20 | Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución |
Country Status (7)
| Country | Link |
|---|---|
| US (8) | US8460879B2 (OSRAM) |
| EP (2) | EP1996717B1 (OSRAM) |
| JP (2) | JP4790026B2 (OSRAM) |
| AU (1) | AU2007217819B2 (OSRAM) |
| CA (1) | CA2643993C (OSRAM) |
| ES (2) | ES2530203T3 (OSRAM) |
| WO (1) | WO2007098148A2 (OSRAM) |
Families Citing this family (115)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006047038A1 (en) * | 2004-09-28 | 2006-05-04 | Trustees Of Tufts College | Apparatus and method for cell migration assays |
| EP2620510B2 (en) * | 2005-06-15 | 2020-02-19 | Complete Genomics Inc. | Single molecule arrays for genetic and chemical analysis |
| US8460879B2 (en) | 2006-02-21 | 2013-06-11 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| US11237171B2 (en) | 2006-02-21 | 2022-02-01 | Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| EP2201374B1 (en) | 2007-08-30 | 2015-10-07 | Trustees Of Tufts College | Methods for determining the concentration of an analyte in solution. |
| US8214646B2 (en) * | 2008-05-06 | 2012-07-03 | Research In Motion Limited | Bundle verification |
| US8222047B2 (en) * | 2008-09-23 | 2012-07-17 | Quanterix Corporation | Ultra-sensitive detection of molecules on single molecule arrays |
| US20100075439A1 (en) * | 2008-09-23 | 2010-03-25 | Quanterix Corporation | Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification |
| US20100075862A1 (en) * | 2008-09-23 | 2010-03-25 | Quanterix Corporation | High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample |
| WO2010039179A1 (en) * | 2008-09-23 | 2010-04-08 | Quanterix Corporation | Ultra-sensitive detection of molecules or enzymes |
| WO2010088547A1 (en) * | 2009-01-29 | 2010-08-05 | Bayer Healthcare Llc | Assays for detecting pegylated blood coagulation factors |
| US9063156B2 (en) * | 2009-06-12 | 2015-06-23 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
| GB0912231D0 (en) * | 2009-07-14 | 2009-08-26 | Imp Innovations Ltd | Method and apparatus for determining an analyte parameter |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| US8415171B2 (en) | 2010-03-01 | 2013-04-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US9678068B2 (en) | 2010-03-01 | 2017-06-13 | Quanterix Corporation | Ultra-sensitive detection of molecules using dual detection methods |
| WO2011109379A1 (en) | 2010-03-01 | 2011-09-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US20120040843A1 (en) | 2010-08-12 | 2012-02-16 | Dublin City University | Centrifugal capture system |
| FR2968314B1 (fr) * | 2010-12-01 | 2016-03-18 | Commissariat Energie Atomique | Procede de detection et de quantification de microorganismes |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| EP2683484A2 (en) | 2011-03-09 | 2014-01-15 | Seng Enterprises Ltd. | Arresting objects |
| US20140302532A1 (en) | 2011-04-12 | 2014-10-09 | Quanterix Corporation | Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury |
| US10941396B2 (en) | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| JP6200948B2 (ja) | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| US9657290B2 (en) * | 2012-07-03 | 2017-05-23 | The Board Of Trustees Of The Leland Stanford Junior University | Scalable bio-element analysis |
| EP4148118A1 (en) | 2012-08-24 | 2023-03-15 | Yale University | System, device and method for high-throughput multi-plexed detection |
| US9932626B2 (en) | 2013-01-15 | 2018-04-03 | Quanterix Corporation | Detection of DNA or RNA using single molecule arrays and other techniques |
| JP6408552B2 (ja) | 2013-03-13 | 2018-10-17 | メソ スケール テクノロジーズ エルエルシー | 改善されたアッセイ方法 |
| US10114015B2 (en) | 2013-03-13 | 2018-10-30 | Meso Scale Technologies, Llc. | Assay methods |
| WO2014143623A1 (en) | 2013-03-15 | 2014-09-18 | The Lubrizol Corporation | Heavy metal free cpvc compositions |
| EP3039158B1 (en) | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| JP6076500B2 (ja) * | 2014-03-31 | 2017-02-08 | 日本碍子株式会社 | 標的物質の検出方法 |
| CN112239774B (zh) | 2014-05-15 | 2024-12-03 | 中尺度技术有限责任公司 | 改进的测定方法 |
| EP3227684B1 (en) | 2014-12-03 | 2019-10-02 | Isoplexis Corporation | Analysis and screening of cell secretion profiles |
| US11353448B2 (en) | 2015-02-13 | 2022-06-07 | California Institute Of Technology | Methods and compositions for quantifying metabolites and proteins from single cells |
| US11434264B2 (en) | 2015-02-13 | 2022-09-06 | Quanterix Corporation | Immunoassays for differential detection of clostridium difficile |
| WO2016134370A1 (en) | 2015-02-22 | 2016-08-25 | The Board Of Trustees Of The Leland Stanford Junior University | Micro-screening apparatus, process, and products |
| US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| JP7508191B2 (ja) | 2015-03-30 | 2024-07-01 | ベクトン・ディキンソン・アンド・カンパニー | コンビナトリアルバーコーディングのための方法および組成物 |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| KR102395450B1 (ko) | 2015-09-11 | 2022-05-09 | 셀룰러 리서치, 인크. | 핵산 라이브러리 정규화를 위한 방법 및 조성물 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| CN109964126B (zh) | 2016-09-12 | 2022-12-27 | 伊索普莱克西斯公司 | 用于细胞治疗法和其他免疫治疗法的多重分析的系统和方法 |
| EP3516400B1 (en) | 2016-09-26 | 2023-08-16 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| JP7348066B2 (ja) | 2016-11-11 | 2023-09-20 | アイソプレキシス コーポレイション | 単一細胞のゲノム、トランスクリプトームおよびプロテオームの同時解析のための組成物および方法 |
| CN115254210A (zh) | 2016-11-14 | 2022-11-01 | 浩康生物系统公司 | 用于分选目标颗粒的方法和装置 |
| WO2018098372A1 (en) | 2016-11-22 | 2018-05-31 | IsoPlexis Corporation | Systems, devices and methods for cell capture and methods of manufacture thereof |
| CN110249082B (zh) | 2016-12-01 | 2023-07-07 | 诺迪勒思附属公司 | 测定蛋白质的方法 |
| US11085039B2 (en) | 2016-12-12 | 2021-08-10 | xCella Biosciences, Inc. | Methods and systems for screening using microcapillary arrays |
| AU2017375631B2 (en) | 2016-12-12 | 2023-06-15 | xCella Biosciences, Inc. | Methods and systems for screening using microcapillary arrays |
| US10324041B2 (en) | 2016-12-21 | 2019-06-18 | Abbott Japan Co., Ltd. | Optical imaging system using lateral illumination for digital assays |
| EP3562796B1 (en) | 2016-12-30 | 2023-04-19 | Xcella Biosciences, Inc. | Multi-stage sample recovery system |
| US11319583B2 (en) | 2017-02-01 | 2022-05-03 | Becton, Dickinson And Company | Selective amplification using blocking oligonucleotides |
| US11047854B2 (en) | 2017-02-06 | 2021-06-29 | Abbott Japan Llc | Methods for reducing noise in signal-generating digital assays |
| EP3580563A4 (en) | 2017-02-09 | 2020-11-18 | Promega Corporation | ANALYTE DETECTION IMMUNOLOGICAL ASSAY |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| WO2019060607A1 (en) | 2017-09-20 | 2019-03-28 | Quanterix Corporation | METHODS AND SYSTEMS FOR DETECTING MOLECULES OF ANALYTES |
| JP7458678B2 (ja) | 2017-12-29 | 2024-04-01 | ノーティラス・サブシディアリー・インコーポレイテッド | タンパク質同定のためのデコーディングアプローチ方法 |
| CN108169480B (zh) * | 2018-02-07 | 2024-03-08 | 上海澜澈生物科技有限公司 | 一种生物标志物的分子数量的检测方法及系统、芯片 |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| CN118910215A (zh) | 2018-05-03 | 2024-11-08 | 贝克顿迪金森公司 | 在相对的转录物末端进行分子条形码化 |
| US12234377B2 (en) | 2018-05-18 | 2025-02-25 | The University Of North Carolina At Chapel Hill | Compositions, devices, and methods for improving a surface property of a substrate |
| WO2020036906A1 (en) * | 2018-08-17 | 2020-02-20 | President And Fellows Of Harvard College | Methods and compositions for detection and quantification of small molecules and other analytes |
| US11639517B2 (en) | 2018-10-01 | 2023-05-02 | Becton, Dickinson And Company | Determining 5′ transcript sequences |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| CA3117813A1 (en) | 2018-12-06 | 2020-06-11 | xCella Biosciences, Inc. | Lateral loading of microcapillary arrays |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| US12071617B2 (en) | 2019-02-14 | 2024-08-27 | Becton, Dickinson And Company | Hybrid targeted and whole transcriptome amplification |
| KR20220010471A (ko) | 2019-03-01 | 2022-01-25 | 메소 스케일 테크놀러지즈, 엘엘시 | 면역검정 방법에서 사용을 위한 전기화학발광성 표지화된 프로브, 이의 사용 방법 및 이를 포함하는 키트 |
| JP7281545B2 (ja) | 2019-07-02 | 2023-05-25 | 旭化成株式会社 | バイオアッセイ用微細ウェルフィルム、バイオアッセイ用微細ウェルフィルム形成用感光性樹脂組成物、及び、バイオアッセイ用微細ウェルフィルムの製造方法 |
| US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
| JP7479051B2 (ja) * | 2019-09-27 | 2024-05-08 | 国立大学法人広島大学 | プラスチック粒子の検出方法およびプラスチック粒子の検出キット |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| WO2021211754A2 (en) | 2020-04-15 | 2021-10-21 | Quanterix Corporation | Methods and systems related to highly sensitive assays and delivering capture objects |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| WO2021242804A1 (en) | 2020-05-26 | 2021-12-02 | Bio-Rad Laboratories, Inc. | Apparatus and method for fast digital detection |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| EP4231174A3 (en) | 2020-06-11 | 2023-11-01 | Nautilus Subsidiary, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| WO2022109343A1 (en) | 2020-11-20 | 2022-05-27 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| EP4281774B1 (en) | 2021-01-20 | 2025-11-26 | Nautilus Subsidiary, Inc. | Biomolecule quantitation |
| US20220227890A1 (en) | 2021-01-21 | 2022-07-21 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule preparation |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| AU2022352593A1 (en) | 2021-09-22 | 2024-02-15 | Nautilus Subsidiary, Inc. | Methods and systems for determining polypeptide interactions |
| US20230149883A1 (en) | 2021-11-03 | 2023-05-18 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| AU2023241893A1 (en) | 2022-03-29 | 2024-09-26 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| US20230360732A1 (en) | 2022-04-25 | 2023-11-09 | Nautilus Subsidiary, Inc. | Systems and methods for assessing and improving the quality of multiplex molecular assays |
| EP4544077A1 (en) | 2022-06-21 | 2025-04-30 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024059655A1 (en) | 2022-09-15 | 2024-03-21 | Nautilus Subsidiary, Inc. | Characterizing accessibility of macromolecule structures |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| WO2024107857A1 (en) | 2022-11-15 | 2024-05-23 | Nautilus Subsidiary, Inc. | Standard polypeptides |
| EP4630816A1 (en) | 2022-12-09 | 2025-10-15 | Nautilus Subsidiary, Inc. | A method comprising performing on a single-analyte array at least 50 cycles of a process |
| EP4634285A1 (en) | 2022-12-15 | 2025-10-22 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| WO2024151373A1 (en) | 2023-01-12 | 2024-07-18 | Nautilus Subsidiary, Inc. | Characterization of glycans and glycoconjugates |
| EP4666071A2 (en) | 2023-02-13 | 2025-12-24 | Nautilus Subsidiary, Inc. | Affinity reagents having artificial polymer scaffolds |
| EP4669966A1 (en) | 2023-02-24 | 2025-12-31 | Nautilus Subsidiary, Inc. | MODIFICATION, SEPARATION AND DETECTION OF PROTEOFORMS |
| WO2024206122A1 (en) | 2023-03-24 | 2024-10-03 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| WO2024216233A1 (en) | 2023-04-13 | 2024-10-17 | Nautilus Subsidiary, Inc. | Artificial proteins for displaying epitopes |
| US20240394902A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| WO2024263696A1 (en) | 2023-06-21 | 2024-12-26 | Nautilus Subsidiary, Inc. | Compositions and methods for improving affinity reagent avidity |
| US20250066841A1 (en) | 2023-08-24 | 2025-02-27 | Nautilus Subsidiary, Inc. | Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions |
| WO2025128588A1 (en) | 2023-12-11 | 2025-06-19 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| US20250205706A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| US20250298016A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| WO2025212338A1 (en) | 2024-04-01 | 2025-10-09 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
Family Cites Families (297)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US423119A (en) * | 1890-03-11 | Punching-machine | ||
| US3212986A (en) | 1964-11-05 | 1965-10-19 | Robert T Pennington | Three tank separate superheat reactor |
| US3712986A (en) | 1969-04-03 | 1973-01-23 | Westinghouse Electric Corp | Electron imaging device utilizing a fiber optic input window |
| SE427505B (sv) | 1977-03-04 | 1983-04-11 | Pharmacia Diagnostics Ab | Reagens till anvendning vid immunkemiska bestemningsmetoder |
| US4200110A (en) | 1977-11-28 | 1980-04-29 | United States Of America | Fiber optic pH probe |
| JPS58219456A (ja) | 1982-06-16 | 1983-12-20 | Aloka Co Ltd | 生化学分析装置 |
| US4558120A (en) | 1983-01-07 | 1985-12-10 | The Dow Chemical Company | Dense star polymer |
| US4631337A (en) | 1983-01-07 | 1986-12-23 | The Dow Chemical Company | Hydrolytically-stable dense star polyamine |
| US4507466A (en) | 1983-01-07 | 1985-03-26 | The Dow Chemical Corporation | Dense star polymers having core, core branches, terminal groups |
| US4568737A (en) | 1983-01-07 | 1986-02-04 | The Dow Chemical Company | Dense star polymers and dendrimers |
| US4737550A (en) | 1983-01-07 | 1988-04-12 | The Dow Chemical Company | Bridged dense star polymers |
| FR2565350B1 (fr) | 1984-06-05 | 1986-10-10 | Paris Nord Universite | Moyens propres a permettre le support, le traitement, le stockage et l'analyse automatiques en continu d'echantillons biologiques |
| US4587329A (en) | 1984-08-17 | 1986-05-06 | The Dow Chemical Company | Dense star polymers having two dimensional molecular diameter |
| US4631211A (en) | 1985-03-25 | 1986-12-23 | Scripps Clinic & Research Foundation | Means for sequential solid phase organic synthesis and methods using the same |
| US4871779A (en) | 1985-12-23 | 1989-10-03 | The Dow Chemical Company | Ion exchange/chelation resins containing dense star polymers having ion exchange or chelate capabilities |
| US4694064A (en) | 1986-02-28 | 1987-09-15 | The Dow Chemical Company | Rod-shaped dendrimer |
| US4780421A (en) | 1986-04-03 | 1988-10-25 | Sclavo Inc. | Cleavable labels for use in binding assays |
| US4713975A (en) | 1986-05-30 | 1987-12-22 | The Dow Chemical Company | Dense star polymers for calibrating/characterizing sub-micron apertures |
| US5252494A (en) | 1986-06-25 | 1993-10-12 | Trustees Of Tufts College | Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix |
| US5560929A (en) | 1986-08-18 | 1996-10-01 | The Dow Chemical Company | Structured copolymers and their use as absorbents, gels and carriers of metal ions |
| US5527524A (en) | 1986-08-18 | 1996-06-18 | The Dow Chemical Company | Dense star polymer conjugates |
| US5338532A (en) | 1986-08-18 | 1994-08-16 | The Dow Chemical Company | Starburst conjugates |
| WO1988005533A1 (en) | 1987-01-16 | 1988-07-28 | Kelsius, Inc. | Amplification of signals from optical fibers |
| FR2613074B1 (fr) | 1987-03-27 | 1990-06-08 | Commissariat Energie Atomique | Capteur chimique actif, a fibres optiques |
| US4962037A (en) | 1987-10-07 | 1990-10-09 | United States Of America | Method for rapid base sequencing in DNA and RNA |
| US4857599A (en) | 1988-02-08 | 1989-08-15 | The Dow Chemical Company | Modified dense star polymers |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| US5028535A (en) | 1989-01-10 | 1991-07-02 | Biosite Diagnostics, Inc. | Threshold ligand-receptor assay |
| US4924870A (en) | 1989-01-13 | 1990-05-15 | Fiberoptic Sensor Technologies, Inc. | Fiber optic sensors |
| US5196306A (en) | 1989-03-29 | 1993-03-23 | E. I. Du Pont De Nemours And Company | Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system |
| US5190857A (en) | 1989-05-19 | 1993-03-02 | Acrogen, Inc. | Optical method for measuring an analyte using area-modulated luminescence |
| US5026159A (en) | 1989-05-19 | 1991-06-25 | Acrogen, Inc. | Area-modulated luminescence (AML) |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5091300A (en) | 1989-08-03 | 1992-02-25 | Merck & Co., Inc. | Radio-immuno assay for hepatitis b virus pres2 antibodies |
| US5152816A (en) | 1989-10-16 | 1992-10-06 | Corning Incorporated | Method of enlarging end of capillary tube bore |
| US5108961A (en) | 1989-12-29 | 1992-04-28 | Circon Corporation | Etchable core glass compositions and method for manufacturing a high performance microchannel plate |
| US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
| US5532138A (en) | 1990-04-26 | 1996-07-02 | Behringwerke Ag | Method and kits for determining peroxidatively active catalysts |
| AU663053B2 (en) | 1990-06-11 | 1995-09-28 | Gilead Sciences, Inc. | Nucleic acid ligands |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US5705337A (en) | 1990-06-11 | 1998-01-06 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chemi-SELEX |
| US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
| US5637459A (en) | 1990-06-11 | 1997-06-10 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chimeric selex |
| US5683867A (en) | 1990-06-11 | 1997-11-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: blended SELEX |
| US5773527A (en) | 1990-08-27 | 1998-06-30 | Dendritech, Inc. | Non-crosslinked, polybranched polymers |
| US5631329A (en) | 1990-08-27 | 1997-05-20 | Dendritech, Inc. | Process for producing hyper-comb-branched polymers |
| US6582908B2 (en) | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
| US5244813A (en) | 1991-01-25 | 1993-09-14 | Trustees Of Tufts College | Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample |
| US5244636A (en) | 1991-01-25 | 1993-09-14 | Trustees Of Tufts College | Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample |
| US5250264A (en) | 1991-01-25 | 1993-10-05 | Trustees Of Tufts College | Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample |
| US5320814A (en) | 1991-01-25 | 1994-06-14 | Trustees Of Tufts College | Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample |
| EP0773227A1 (en) | 1991-09-18 | 1997-05-14 | Affymax Technologies N.V. | Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides |
| US6943034B1 (en) | 1991-11-22 | 2005-09-13 | Affymetrix, Inc. | Combinatorial strategies for polymer synthesis |
| US5315375A (en) | 1992-02-11 | 1994-05-24 | Acrogen, Inc. | Sensitive light detection system |
| US6113946A (en) | 1992-04-03 | 2000-09-05 | The Regents Of The University Of California | Self-assembling polynucleotide delivery system comprising dendrimer polycations |
| HU9201687D0 (en) | 1992-05-21 | 1992-08-28 | Arpad Furka | Preparation of sets from peptid mixtures with polycomponents and their use for the identification of biologically active peptides |
| US6156270A (en) | 1992-05-21 | 2000-12-05 | Biosite Diagnostics, Inc. | Diagnostic devices and apparatus for the controlled movement of reagents without membranes |
| US5329461A (en) | 1992-07-23 | 1994-07-12 | Acrogen, Inc. | Digital analyte detection system |
| US5298741A (en) | 1993-01-13 | 1994-03-29 | Trustees Of Tufts College | Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample |
| US7241569B2 (en) | 1993-01-18 | 2007-07-10 | Olympus Corporation | Method and a device for the evaluation of biopolymer fitness |
| US5387617A (en) | 1993-01-22 | 1995-02-07 | The Dow Chemical Company | Small cell foams and blends and a process for their preparation |
| AU6267794A (en) | 1993-02-24 | 1994-09-14 | Dade International Inc. | Immobilization of specific binding assay reagents |
| US5898005A (en) | 1993-02-24 | 1999-04-27 | Dade Behring Inc. | Rapid detection of analytes with receptors immobilized on soluble submicron particles |
| JP3441763B2 (ja) | 1993-06-30 | 2003-09-02 | キリン−アムジエン・インコーポレーテツド | モノクローナル抗体及び該モノクローナル抗体を用いたヒトｇ−ｃｓｆの測定法 |
| US6087186A (en) | 1993-07-16 | 2000-07-11 | Irori | Methods and apparatus for synthesizing labeled combinatorial chemistry libraries |
| US5374395A (en) | 1993-10-14 | 1994-12-20 | Amoco Corporation | Diagnostics instrument |
| US6015880A (en) | 1994-03-16 | 2000-01-18 | California Institute Of Technology | Method and substrate for performing multiple sequential reactions on a matrix |
| JPH10500951A (ja) | 1994-05-23 | 1998-01-27 | スミスクライン・ビーチャム・コーポレイション | コードされた組合せライブラリー |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| WO1995035506A2 (en) | 1994-06-17 | 1995-12-28 | Kensington Laboratories, Inc. | Scribe mark reader |
| US5512490A (en) | 1994-08-11 | 1996-04-30 | Trustees Of Tufts College | Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns |
| US6001564A (en) | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US5620850A (en) | 1994-09-26 | 1997-04-15 | President And Fellows Of Harvard College | Molecular recognition at surfaces derivatized with self-assembled monolayers |
| US6013445A (en) | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
| US20030104361A1 (en) | 1997-09-29 | 2003-06-05 | Susan Weininger | Method of detection of nucleic acids with a specific sequence composition |
| US6884357B2 (en) | 1995-02-21 | 2005-04-26 | Iqbal Waheed Siddiqi | Apparatus and method for processing magnetic particles |
| US6329139B1 (en) | 1995-04-25 | 2001-12-11 | Discovery Partners International | Automated sorting system for matrices with memory |
| US5532379A (en) | 1995-05-05 | 1996-07-02 | Pierce Chemical Company | Biotin containing heterobifunctional cleavable compounds |
| US5690894A (en) | 1995-05-23 | 1997-11-25 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
| US5545531A (en) | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
| IL115465A0 (en) | 1995-09-29 | 1995-12-31 | Yeda Res & Dev | Assay for the diagnosis of dementia |
| US5981180A (en) | 1995-10-11 | 1999-11-09 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and methods |
| DE19540098C2 (de) | 1995-10-27 | 2001-05-17 | Inst Chemo Biosensorik | Verfahren und Mehrkanalbiosensor zur Mehrkomponentenanalyse von Mischungen und/oder Gemischen |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6143495A (en) | 1995-11-21 | 2000-11-07 | Yale University | Unimolecular segment amplification and sequencing |
| US5633972A (en) | 1995-11-29 | 1997-05-27 | Trustees Of Tufts College | Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy |
| US5641634A (en) | 1995-11-30 | 1997-06-24 | Mandecki; Wlodek | Electronically-indexed solid-phase assay for biomolecules |
| US5814524A (en) | 1995-12-14 | 1998-09-29 | Trustees Of Tufts College | Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations |
| US5846839A (en) | 1995-12-22 | 1998-12-08 | Glaxo Group Limited | Methods for hard-tagging an encoded synthetic library |
| US5837196A (en) | 1996-01-26 | 1998-11-17 | The Regents Of The University Of California | High density array fabrication and readout method for a fiber optic biosensor |
| US6586193B2 (en) | 1996-04-25 | 2003-07-01 | Genicon Sciences Corporation | Analyte assay using particulate labels |
| US6387707B1 (en) | 1996-04-25 | 2002-05-14 | Bioarray Solutions | Array Cytometry |
| US5824478A (en) | 1996-04-30 | 1998-10-20 | Vysis, Inc. | Diagnostic methods and probes |
| US5885529A (en) | 1996-06-28 | 1999-03-23 | Dpc Cirrus, Inc. | Automated immunoassay analyzer |
| US7169556B2 (en) | 1996-07-29 | 2007-01-30 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| EP0925494B1 (en) | 1996-09-04 | 2001-12-19 | Scandinavian Micro Biodevices A/S | A micro flow system for particle separation and analysis |
| US5900481A (en) | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
| US6133436A (en) | 1996-11-06 | 2000-10-17 | Sequenom, Inc. | Beads bound to a solid support and to nucleic acids |
| JP2001524808A (ja) | 1996-12-10 | 2001-12-04 | ジーントレイス・システムズ・インコーポレイテッド | 放出可能な不揮発性の質量標識分子 |
| SE9700384D0 (sv) | 1997-02-04 | 1997-02-04 | Biacore Ab | Analytical method and apparatus |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| US20030027126A1 (en) | 1997-03-14 | 2003-02-06 | Walt David R. | Methods for detecting target analytes and enzymatic reactions |
| US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
| US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
| US6406845B1 (en) | 1997-05-05 | 2002-06-18 | Trustees Of Tuft College | Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample |
| US6203983B1 (en) | 1997-06-16 | 2001-03-20 | Affymetrix, Inc. | Method for detecting chemical interactions between naturally occurring bio-polymers which are non-identical binding partners |
| FR2764989B1 (fr) | 1997-06-20 | 1999-08-27 | Pasteur Sanofi Diagnostics | Procede de dosage du c-peptide |
| US6174695B1 (en) | 1997-08-12 | 2001-01-16 | The Regents Of The University Of California | Epoxide hydrolase inhibitor methods |
| US6838051B2 (en) | 1999-05-03 | 2005-01-04 | Ljl Biosystems, Inc. | Integrated sample-processing system |
| US5987180A (en) | 1997-09-26 | 1999-11-16 | Sarnoff Corporation | Multiple component compression encoder motion search method and apparatus |
| US7115884B1 (en) | 1997-10-06 | 2006-10-03 | Trustees Of Tufts College | Self-encoding fiber optic sensor |
| US7348181B2 (en) | 1997-10-06 | 2008-03-25 | Trustees Of Tufts College | Self-encoding sensor with microspheres |
| US6878345B1 (en) | 1997-12-08 | 2005-04-12 | Thomas W. Astle | Ultra high throughput bioassay screening system |
| SE9800360D0 (sv) | 1998-02-06 | 1998-02-06 | Goeteborg University Science I | Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules |
| US6210910B1 (en) | 1998-03-02 | 2001-04-03 | Trustees Of Tufts College | Optical fiber biosensor array comprising cell populations confined to microcavities |
| EP1066414B1 (en) | 1998-03-25 | 2009-06-10 | Olink AB | Rolling circle replication of padlock probes |
| US6713908B1 (en) * | 1998-03-31 | 2004-03-30 | California Institute Of Technology | Using a micromachined magnetostatic relay in commutating a DC motor |
| EP1188059A2 (en) | 1998-05-13 | 2002-03-20 | Accelr8 Technology Corporation | Enumeration method of analyte detection |
| EP2360271A1 (en) | 1998-06-24 | 2011-08-24 | Illumina, Inc. | Decoding of array sensors with microspheres |
| US6908770B1 (en) | 1998-07-16 | 2005-06-21 | Board Of Regents, The University Of Texas System | Fluid based analysis of multiple analytes by a sensor array |
| ATE357289T1 (de) | 1998-08-28 | 2007-04-15 | Febit Biotech Gmbh | Träger für analytbestimmungsverfahren und verfahren zur herstellung des trägers |
| US5971180A (en) * | 1998-09-02 | 1999-10-26 | Wu; Mao Sheng | Sealing plug cap for a sealing container |
| US6285807B1 (en) | 1998-11-16 | 2001-09-04 | Trustees Of Tufts College | Fiber optic sensor for long-term analyte measurements in fluids |
| DE19854003A1 (de) | 1998-11-18 | 2000-05-25 | Jenoptik Jena Gmbh | Simultanes Magnetpartikelhandling in zweidimensionaler Anordnung |
| US6429027B1 (en) | 1998-12-28 | 2002-08-06 | Illumina, Inc. | Composite arrays utilizing microspheres |
| JP2002536665A (ja) | 1999-02-09 | 2002-10-29 | イルミナ インコーポレイテッド | ランダムに並んだアレイにおける自動情報処理 |
| ATE341003T1 (de) | 1999-02-16 | 2006-10-15 | Applera Corp | Vorrichtung zur handhabung von kügelchen |
| JP2002544488A (ja) | 1999-05-07 | 2002-12-24 | クアンタム ドット コーポレイション | 半導体ナノクリスタルを用いて分析物を検出する方法 |
| DE60030436T2 (de) | 1999-05-20 | 2007-03-29 | Illumina, Inc., San Diego | Vorrichtung zur halterung und präsentation von mindestens einer mikrokugelmatrix zu lösungen und/oder zu optischen abbildungssystemen |
| EP1190100B1 (en) * | 1999-05-20 | 2012-07-25 | Illumina, Inc. | Combinatorial decoding of random nucleic acid arrays |
| US8080380B2 (en) | 1999-05-21 | 2011-12-20 | Illumina, Inc. | Use of microfluidic systems in the detection of target analytes using microsphere arrays |
| US6706519B1 (en) | 1999-06-22 | 2004-03-16 | Tecan Trading Ag | Devices and methods for the performance of miniaturized in vitro amplification assays |
| CA2379130A1 (en) | 1999-07-16 | 2001-01-25 | Board Of Regents, The University Of Texas System | Method and apparatus for the delivery of samples to a chemical sensor array |
| US6713309B1 (en) | 1999-07-30 | 2004-03-30 | Large Scale Proteomics Corporation | Microarrays and their manufacture |
| EP1212599A2 (en) | 1999-08-30 | 2002-06-12 | Illumina, Inc. | Methods for improving signal detection from an array |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US7211390B2 (en) | 1999-09-16 | 2007-05-01 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| JP2001249079A (ja) | 1999-12-28 | 2001-09-14 | Fujirebio Inc | 発光量調節剤を用いた化学発光測定方法及び酵素免疫測定方法 |
| AU2001234709A1 (en) | 2000-01-31 | 2001-08-07 | Board Of Regents, The University Of Texas System | Method of preparing a sensor array |
| DE10005301A1 (de) | 2000-02-07 | 2001-08-09 | Trutnau Hans Heinrich | Multi-Schritt-Kinetik molekularer Interaktionen als analytisches Mess- und Auswerte-Verfahren |
| AU2001247417A1 (en) | 2000-03-14 | 2001-09-24 | Trustees Of Tufts College | Cross-reactive sensors |
| JP2001269196A (ja) | 2000-03-24 | 2001-10-02 | Hamamatsu Photonics Kk | 被検体核酸の定量方法、および被検体核酸の分子数の計数方法 |
| US6734420B2 (en) | 2000-04-06 | 2004-05-11 | Quantum Dot Corporation | Differentiable spectral bar code methods and systems |
| US20020090650A1 (en) | 2000-04-06 | 2002-07-11 | Quantum Dot Corporation | Two-dimensional spectral imaging system |
| AU2001293336A1 (en) | 2000-04-11 | 2001-10-23 | Ulf Landegren | Nucleic acid detection medium |
| US7682837B2 (en) | 2000-05-05 | 2010-03-23 | Board Of Trustees Of Leland Stanford Junior University | Devices and methods to form a randomly ordered array of magnetic beads and uses thereof |
| US20040009614A1 (en) | 2000-05-12 | 2004-01-15 | Ahn Chong H | Magnetic bead-based arrays |
| US6471968B1 (en) | 2000-05-12 | 2002-10-29 | Regents Of The University Of Michigan | Multifunctional nanodevice platform |
| WO2005000087A2 (en) * | 2003-06-03 | 2005-01-06 | Chiron Corporation | Gene products differentially expressed in cancerous colon cells and their methods of use ii |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
| EP1180679B1 (en) | 2000-08-14 | 2012-10-10 | Evotec AG | A method for sensing fluorescent samples utilizing moment analysis |
| WO2002027031A2 (en) | 2000-09-28 | 2002-04-04 | Cellomics, Inc. | Methods and reagents for live-cell gene expression quantification |
| US20060068409A1 (en) | 2000-11-27 | 2006-03-30 | Phan Brigitte C | Use of restriction enzymes and other chemical methods to decrease non-specific binding in dual bead assays and related bio-discs, methods, and system apparatus for detecting medical targets |
| EP1336106B1 (en) | 2001-02-15 | 2004-11-17 | Unibio S.R.L. | Diagnostic method for bacterial vaginosis based on detecting antibodies against gvh toxin of gardnerella vaginalis |
| US6573051B2 (en) | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
| US20030162236A1 (en) | 2001-03-26 | 2003-08-28 | Response Biomedical Corporation | Compensation for variability in specific binding in quantitative assays |
| US20040091939A1 (en) | 2001-03-28 | 2004-05-13 | To Cheung | Device and method for detection of multiple analytes |
| US7060441B2 (en) | 2001-05-04 | 2006-06-13 | Biomerieux | Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling |
| US20030008313A1 (en) | 2001-06-19 | 2003-01-09 | Wiltshire Richard S. | Process for enhanced molecular target detection using layered rolling circle amplification |
| US6991939B2 (en) | 2001-07-19 | 2006-01-31 | Tufts University | Optical array device and methods of use thereof for screening, analysis and manipulation of particles |
| US20040241688A1 (en) | 2001-07-19 | 2004-12-02 | Cuneyt Bukusoglu | Human tissue specific drug screening procedure |
| GB2378245A (en) | 2001-08-03 | 2003-02-05 | Mats Nilsson | Nucleic acid amplification method |
| US20030040129A1 (en) | 2001-08-20 | 2003-02-27 | Shah Haresh P. | Binding assays using magnetically immobilized arrays |
| US6616554B2 (en) * | 2001-08-22 | 2003-09-09 | Grace Liao | Training device for baseball hitting |
| JP2005518553A (ja) | 2001-09-06 | 2005-06-23 | ジェノミック プロファイリング システムズ インコーポレイティッド | 細胞およびウイルスの迅速かつ高感度な検出方法 |
| US20030091475A1 (en) | 2001-09-26 | 2003-05-15 | Micralyne Inc. | Bead trapping device |
| US7195913B2 (en) | 2001-10-05 | 2007-03-27 | Surmodics, Inc. | Randomly ordered arrays and methods of making and using |
| US6902921B2 (en) | 2001-10-30 | 2005-06-07 | 454 Corporation | Sulfurylase-luciferase fusion proteins and thermostable sulfurylase |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| US7632671B2 (en) | 2001-11-29 | 2009-12-15 | Sun-Wing Tong | Molecular detection and assay by electrobiochip micro-array |
| WO2003052173A1 (en) | 2001-12-14 | 2003-06-26 | Corning Incorporated | Two step etching process for an optical fiber preform |
| US20030119203A1 (en) | 2001-12-24 | 2003-06-26 | Kimberly-Clark Worldwide, Inc. | Lateral flow assay devices and methods for conducting assays |
| WO2003065019A1 (en) | 2002-01-25 | 2003-08-07 | Illumina, Inc. | Sensor arrays for detecting analytes in fluids |
| US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
| WO2003071223A2 (en) | 2002-02-19 | 2003-08-28 | Tufts University | Electro-optical stimulation/measurement |
| US7232547B2 (en) | 2002-03-22 | 2007-06-19 | Marshfield Clinic | Apparatus and method for testing and continuously reading low-volume samples |
| US7312085B2 (en) | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| WO2003089901A2 (en) | 2002-04-19 | 2003-10-30 | Palsson Bernhard O | Methods for preparing libraries of unique tags and related screening methods |
| PL374438A1 (en) | 2002-05-10 | 2005-10-17 | Pharmacia Corporation | Peptide compounds and their use as protease substrates |
| CA2487126A1 (en) | 2002-05-24 | 2003-12-04 | F. Sperling Aps | A method for testing the interaction between at least one liquid sample and a respective solid sample |
| US7387889B2 (en) | 2002-08-22 | 2008-06-17 | Massachusetts Institute Of Technology | Measurement of concentrations and binding energetics |
| US7285424B2 (en) | 2002-08-27 | 2007-10-23 | Kimberly-Clark Worldwide, Inc. | Membrane-based assay devices |
| US7682688B2 (en) | 2002-11-26 | 2010-03-23 | University Of Utah Research Foundation | Microporous materials, methods, and articles for localizing and quantifying analytes |
| US7597936B2 (en) | 2002-11-26 | 2009-10-06 | University Of Utah Research Foundation | Method of producing a pigmented composite microporous material |
| US7046888B2 (en) | 2002-12-18 | 2006-05-16 | The Regents Of The University Of Michigan | Enhancing fiber-optic sensing technique using a dual-core fiber |
| WO2004057386A1 (en) | 2002-12-18 | 2004-07-08 | The Regents Of The University Of Michigan | Enhancing fiber-optic sensing technique using a dual-core fiber |
| CA2510166A1 (en) | 2002-12-20 | 2004-09-30 | Caliper Life Sciences, Inc. | Single molecule amplification and detection of dna |
| US20050221281A1 (en) | 2003-01-08 | 2005-10-06 | Ho Winston Z | Self-contained microfluidic biochip and apparatus |
| CA2513985C (en) | 2003-01-21 | 2012-05-29 | Illumina Inc. | Chemical reaction monitor |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
| US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
| AU2004228655A1 (en) | 2003-04-02 | 2004-10-21 | Northwestern University | Methods of controlling nanoparticle growth |
| AU2004228678A1 (en) | 2003-04-03 | 2004-10-21 | Fluidigm Corp. | Microfluidic devices and methods of using same |
| US7148043B2 (en) | 2003-05-08 | 2006-12-12 | Bio-Rad Laboratories, Inc. | Systems and methods for fluorescence detection with a movable detection module |
| JP2004354164A (ja) | 2003-05-28 | 2004-12-16 | Olympus Corp | 微粒子を用いた検体の検査方法及びその検査システム |
| US6999657B2 (en) | 2003-05-28 | 2006-02-14 | Tufts University | High density optical data storage |
| US20040248103A1 (en) | 2003-06-04 | 2004-12-09 | Feaver William John | Proximity-mediated rolling circle amplification |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| WO2005019419A2 (en) | 2003-07-31 | 2005-03-03 | Singulex, Inc. | Co-detection of single polypeptide and polynucleotide molecules |
| US8022013B2 (en) | 2003-08-29 | 2011-09-20 | Illumina, Inc. | Method of forming and using solid-phase support |
| WO2005029041A2 (en) | 2003-09-19 | 2005-03-31 | Applera Corporation | High density sequence detection methods and apparatus |
| US20050226780A1 (en) | 2003-09-19 | 2005-10-13 | Donald Sandell | Manual seal applicator |
| SE0302525D0 (sv) | 2003-09-24 | 2003-09-24 | Biacore Ab | Method and system for interaction analysis |
| JP2007533971A (ja) | 2003-09-30 | 2007-11-22 | シンギュレックス・インコーポレイテッド | 粒子検出の分析精度を改善する方法 |
| JP2007518065A (ja) | 2003-10-28 | 2007-07-05 | バイオアレイ ソリューションズ リミテッド | 吸着又は結合生体分子を有するゲル−シェルビード |
| WO2005050224A2 (en) | 2003-11-13 | 2005-06-02 | Epitome Biosystems Inc. | Small molecule and peptide arrays and uses thereof |
| US20060019264A1 (en) | 2003-12-01 | 2006-01-26 | Said Attiya | Method for isolation of independent, parallel chemical micro-reactions using a porous filter |
| JP2007533305A (ja) | 2004-03-03 | 2007-11-22 | ザ ジェネラル ホスピタル コーポレーション | 微小流動環境において細胞および生体分子を単離するための磁気装置 |
| US8634072B2 (en) | 2004-03-06 | 2014-01-21 | Michael Trainer | Methods and apparatus for determining characteristics of particles |
| WO2005095262A1 (en) | 2004-04-01 | 2005-10-13 | Nanyang Technological University | Microchip and method for detecting molecules and molecular interactions |
| US8961900B2 (en) | 2004-04-28 | 2015-02-24 | Yokogawa Electric Corporation | Chemical reaction cartridge, method of producing chemical reaction cartridge, and mechanism for driving chemical reaction cartridge |
| DE102004021780B4 (de) | 2004-04-30 | 2008-10-02 | Siemens Ag | Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien |
| CA2565166C (en) | 2004-05-12 | 2017-12-12 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
| US6999647B2 (en) * | 2004-05-25 | 2006-02-14 | Honeywell International Inc. | Fast pivot mechanism |
| US7307802B2 (en) | 2004-06-07 | 2007-12-11 | Fluidigm Corporation | Optical lens system and method for microfluidic devices |
| CA2475240A1 (en) * | 2004-07-20 | 2006-01-20 | Biophys, Inc. | Method and device to measure dynamic internal calibration true dose response curves |
| KR100806568B1 (ko) | 2004-08-21 | 2008-02-28 | 주식회사 엘지생명과학 | 미세 유체 소자 및 이를 구비한 진단 및 분석 장치 |
| CN1286987C (zh) | 2004-09-23 | 2006-11-29 | 湖南大学 | 一维生物芯片及其在基因、蛋白表达分析中的应用 |
| WO2006047038A1 (en) | 2004-09-28 | 2006-05-04 | Trustees Of Tufts College | Apparatus and method for cell migration assays |
| US8685711B2 (en) | 2004-09-28 | 2014-04-01 | Singulex, Inc. | Methods and compositions for highly sensitive detection of molecules |
| US7572640B2 (en) | 2004-09-28 | 2009-08-11 | Singulex, Inc. | Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties |
| JP2008514955A (ja) | 2004-09-28 | 2008-05-08 | シンギュレックス・インコーポレイテッド | サンプル分析システムおよび方法 |
| WO2006055735A2 (en) | 2004-11-16 | 2006-05-26 | Illumina, Inc | Scanner having spatial light modulator |
| US20090068162A1 (en) | 2004-11-17 | 2009-03-12 | Robert Sack | Stationary Phase Antibody Arrays for Trace Protein Analysis |
| EP1851554A2 (en) | 2005-01-26 | 2007-11-07 | Enigma Diagnostics Ltd | Method for carrying out a multi-step reaction, breakable container for storing reagents and method for transferring solid reagent using an electrostatically charged wand |
| WO2006102297A1 (en) | 2005-03-22 | 2006-09-28 | Applera Corporation | Automated seal applicator |
| US7682816B2 (en) | 2005-04-07 | 2010-03-23 | 454 Life Sciences Corporation | Thin film coated microwell arrays and methods of using same |
| CN101238221B (zh) | 2005-04-12 | 2011-11-16 | Novia公司名下的现场Rcp公司 | 新型环形探针及其在鉴定生物分子中的用途 |
| EP1871897B1 (en) | 2005-04-12 | 2010-09-29 | Olink AB | Methods for production of oligonucleotides |
| EP1721657A1 (en) | 2005-05-13 | 2006-11-15 | SONY DEUTSCHLAND GmbH | A method of fabricating a polymeric membrane having at least one pore |
| EP2703499A1 (en) | 2005-06-02 | 2014-03-05 | Fluidigm Corporation | Analysis using microfluidic partitioning devices to generate single cell samples |
| US20070125942A1 (en) | 2005-07-06 | 2007-06-07 | The Regents Of The University Of California | Apparatuses, systems and methods for isolating and separating biological materials |
| US7595473B2 (en) | 2005-08-22 | 2009-09-29 | Tufts University | Method and system of array imaging |
| WO2007033385A2 (en) | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
| AU2006336290A1 (en) | 2005-09-16 | 2007-07-26 | The Regents Of The University Of California | A colorimetric bio-barcode amplification assay for analyte detection |
| WO2007035633A2 (en) | 2005-09-16 | 2007-03-29 | President & Fellows Of Harvard College | Screening assays and methods |
| WO2007044974A2 (en) | 2005-10-12 | 2007-04-19 | The Research Foundation Of State University Of New York | Absolute pcr quantification |
| US20070116607A1 (en) | 2005-11-23 | 2007-05-24 | Pharmacom Microlelectronics, Inc. | Microsystems that integrate three-dimensional microarray and multi-layer microfluidics for combinatorial detection of bioagent at single molecule level |
| WO2007072290A2 (en) | 2005-12-21 | 2007-06-28 | Koninklijke Philips Electronics N.V. | Method of performing a microarray assay |
| US20090246795A1 (en) | 2005-12-22 | 2009-10-01 | Rohm Co., Ltd. | Immunoassay device and method |
| CA2636855C (en) | 2006-01-11 | 2016-09-27 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
| GB0600927D0 (en) | 2006-01-17 | 2006-02-22 | Glaxosmithkline Biolog Sa | Assay and materials therefor |
| US8460879B2 (en) | 2006-02-21 | 2013-06-11 | The Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| US11237171B2 (en) | 2006-02-21 | 2022-02-01 | Trustees Of Tufts College | Methods and arrays for target analyte detection and determination of target analyte concentration in solution |
| US20100255518A1 (en) | 2006-04-04 | 2010-10-07 | Goix Philippe J | Highly sensitive system and methods for analysis of troponin |
| US7838250B1 (en) | 2006-04-04 | 2010-11-23 | Singulex, Inc. | Highly sensitive system and methods for analysis of troponin |
| US8470606B2 (en) | 2006-04-18 | 2013-06-25 | Duke University | Manipulation of beads in droplets and methods for splitting droplets |
| US7759062B2 (en) | 2006-06-09 | 2010-07-20 | Third Wave Technologies, Inc. | T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection |
| WO2008016335A1 (en) | 2006-08-03 | 2008-02-07 | National University Of Singapore | A microarray system and a process for producing microarrays |
| CN1928561A (zh) | 2006-09-15 | 2007-03-14 | 东南大学 | 基于光子晶体微球的多元免疫检测方法 |
| EP2089712A4 (en) | 2006-11-22 | 2010-09-22 | Life Technologies Corp | BIOMARKERS OF AUTOIMMUNE DISEASES |
| EP2111303B1 (de) | 2007-01-26 | 2014-10-15 | Haas-Mondomix B.V. | Vorrichtung und verfahren zum dosieren geschäumter massen |
| AU2008276024A1 (en) | 2007-07-16 | 2009-01-22 | California Institute Of Technology | Microfluidic devices, methods and systems for detecting target molecules |
| US20090053719A1 (en) | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
| US20090087860A1 (en) | 2007-08-24 | 2009-04-02 | Todd John A | Highly sensitive system and methods for analysis of prostate specific antigen (psa) |
| EP2201374B1 (en) | 2007-08-30 | 2015-10-07 | Trustees Of Tufts College | Methods for determining the concentration of an analyte in solution. |
| US8093063B2 (en) | 2007-11-29 | 2012-01-10 | Quest Diagnostics Investments Incorporated | Assay for detecting genetic abnormalities in genomic nucleic acids |
| US20090254180A1 (en) | 2008-01-14 | 2009-10-08 | Walt Pazanowski | Customizable vertebral replacement assembly system |
| US9487822B2 (en) | 2008-03-19 | 2016-11-08 | Fluidigm Corporation | Method and apparatus for determining copy number variation using digital PCR |
| US20100189338A1 (en) | 2008-04-09 | 2010-07-29 | Nexcelom Bioscience | Systems and methods for counting cells and biomolecules |
| ITTO20080314A1 (it) | 2008-04-23 | 2009-10-24 | Milano Politecnico | Biosensori spintronici con area attiva localizzata su una parete di dominio magnetico. |
| US8093064B2 (en) | 2008-05-15 | 2012-01-10 | The Regents Of The University Of California | Method for using magnetic particles in droplet microfluidics |
| US20090307772A1 (en) | 2008-05-21 | 2009-12-10 | Honeywell International Inc. | framework for scalable state estimation using multi network observations |
| US20090289834A1 (en) | 2008-05-23 | 2009-11-26 | Honeywell International Inc. | High integrity radio altimeter |
| US8297355B2 (en) | 2008-08-22 | 2012-10-30 | Texaco Inc. | Using heat from produced fluids of oil and gas operations to produce energy |
| US8222047B2 (en) | 2008-09-23 | 2012-07-17 | Quanterix Corporation | Ultra-sensitive detection of molecules on single molecule arrays |
| US20100075355A1 (en) | 2008-09-23 | 2010-03-25 | Quanterix Corporation | Ultra-sensitive detection of enzymes by capture-and-release followed by quantification |
| US20100075862A1 (en) | 2008-09-23 | 2010-03-25 | Quanterix Corporation | High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample |
| US20100075439A1 (en) | 2008-09-23 | 2010-03-25 | Quanterix Corporation | Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification |
| US20100204335A1 (en) | 2008-12-01 | 2010-08-12 | Allergan, Inc. | Kit and composition for eyelash growth |
| TWI373617B (en) | 2009-03-03 | 2012-10-01 | Univ Nat Taiwan | Compact disk based system for separating immunomagnetic bead labeled particulates and method thereof |
| US8415171B2 (en) | 2010-03-01 | 2013-04-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| US9678068B2 (en) | 2010-03-01 | 2017-06-13 | Quanterix Corporation | Ultra-sensitive detection of molecules using dual detection methods |
| US8236574B2 (en) | 2010-03-01 | 2012-08-07 | Quanterix Corporation | Ultra-sensitive detection of molecules or particles using beads or other capture objects |
| WO2011109379A1 (en) | 2010-03-01 | 2011-09-09 | Quanterix Corporation | Methods and systems for extending dynamic range in assays for the detection of molecules or particles |
| EP2663654A1 (en) | 2011-01-14 | 2013-11-20 | Life Technologies Corporation | Methods, compositions, and kits for detecting rare cells |
| US9952237B2 (en) | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
| US20140302532A1 (en) | 2011-04-12 | 2014-10-09 | Quanterix Corporation | Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury |
| WO2012142300A2 (en) | 2011-04-12 | 2012-10-18 | Quanterix Corporation | Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury resulting from a hypoxic event |
| DE202012013668U1 (de) | 2011-06-02 | 2019-04-18 | Raindance Technologies, Inc. | Enzymquantifizierung |
| WO2012170776A2 (en) | 2011-06-09 | 2012-12-13 | Quanterix Corporation | Methods of determining a patient's prognosis for recurrence of prostate cancer and/or determining a course of treatment for prostate cancer following a radical prostatectomy |
| US9932626B2 (en) | 2013-01-15 | 2018-04-03 | Quanterix Corporation | Detection of DNA or RNA using single molecule arrays and other techniques |
| WO2014183096A1 (en) | 2013-05-09 | 2014-11-13 | Quanterix Corporation | Methods, materials, and kits for covalently associating molecular species with a surface of an object |
| US9482622B2 (en) | 2014-05-16 | 2016-11-01 | Massachusetts Institute Of Technology | Methods and apparatus for surface classification |
| WO2016115256A1 (en) | 2015-01-13 | 2016-07-21 | Quanterix Corporation | Methods relating to improving accuracy of capture object-based assays |
| US11434264B2 (en) | 2015-02-13 | 2022-09-06 | Quanterix Corporation | Immunoassays for differential detection of clostridium difficile |
| US9809163B2 (en) | 2015-04-14 | 2017-11-07 | Harman International Industries, Incorporation | Techniques for transmitting an alert towards a target area |
| US20200123592A1 (en) | 2017-05-30 | 2020-04-23 | Quanterix Corporation | Single molecule detection and quantification of nucleic acids with single base specificity |
| WO2019060607A1 (en) | 2017-09-20 | 2019-03-28 | Quanterix Corporation | METHODS AND SYSTEMS FOR DETECTING MOLECULES OF ANALYTES |
| US20200032326A1 (en) | 2018-05-29 | 2020-01-30 | Trustees Of Tufts College | Ultrasensitive assays for detection of short nucleic acids |
| US20220205992A1 (en) | 2020-12-28 | 2022-06-30 | Quanterix Corporation | Materials and kits relating to association of reporter species and targeting entities with beads |
-
2007
- 2007-02-16 US US11/707,385 patent/US8460879B2/en active Active
- 2007-02-16 US US11/707,383 patent/US8460878B2/en active Active
- 2007-02-16 US US11/707,384 patent/US8492098B2/en active Active
- 2007-02-20 WO PCT/US2007/004349 patent/WO2007098148A2/en not_active Ceased
- 2007-02-20 ES ES07751131.9T patent/ES2530203T3/es active Active
- 2007-02-20 EP EP07751131.9A patent/EP1996717B1/en active Active
- 2007-02-20 ES ES12177276.8T patent/ES2613083T3/es active Active
- 2007-02-20 EP EP12177276.8A patent/EP2538220B1/en active Active
- 2007-02-20 JP JP2008556383A patent/JP4790026B2/ja active Active
- 2007-02-20 AU AU2007217819A patent/AU2007217819B2/en active Active
- 2007-02-20 CA CA2643993A patent/CA2643993C/en active Active
-
2011
- 2011-02-18 JP JP2011034007A patent/JP5410464B2/ja active Active
-
2013
- 2013-04-25 US US13/870,596 patent/US20130345078A1/en not_active Abandoned
-
2015
- 2015-03-04 US US14/638,245 patent/US9395359B2/en active Active
-
2016
- 2016-07-08 US US15/205,497 patent/US10261089B2/en active Active
-
2021
- 2021-10-08 US US17/496,926 patent/US11874279B2/en active Active
-
2023
- 2023-11-22 US US18/518,117 patent/US20240159765A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8492098B2 (en) | 2013-07-23 |
| EP2538220A1 (en) | 2012-12-26 |
| US20070259448A1 (en) | 2007-11-08 |
| US10261089B2 (en) | 2019-04-16 |
| US20070259381A1 (en) | 2007-11-08 |
| JP5410464B2 (ja) | 2014-02-05 |
| AU2007217819A1 (en) | 2007-08-30 |
| AU2007217819B2 (en) | 2013-09-19 |
| ES2613083T3 (es) | 2017-05-22 |
| EP1996717A2 (en) | 2008-12-03 |
| JP2009529658A (ja) | 2009-08-20 |
| WO2007098148A2 (en) | 2007-08-30 |
| US8460879B2 (en) | 2013-06-11 |
| CA2643993A1 (en) | 2007-08-30 |
| EP1996717B1 (en) | 2014-11-12 |
| JP2011137830A (ja) | 2011-07-14 |
| US20150233905A1 (en) | 2015-08-20 |
| CA2643993C (en) | 2015-12-15 |
| US20130345078A1 (en) | 2013-12-26 |
| JP4790026B2 (ja) | 2011-10-12 |
| US9395359B2 (en) | 2016-07-19 |
| WO2007098148A3 (en) | 2008-10-16 |
| US20220099678A1 (en) | 2022-03-31 |
| US8460878B2 (en) | 2013-06-11 |
| EP2538220B1 (en) | 2016-11-09 |
| US20170038390A1 (en) | 2017-02-09 |
| US11874279B2 (en) | 2024-01-16 |
| US20240159765A1 (en) | 2024-05-16 |
| US20070259385A1 (en) | 2007-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2530203T3 (es) | Métodos y matrices para detección de analito objeto de estudio y determinación de concentración de analito objeto de estudio en disolución | |
| Jans et al. | Gold nanoparticle-enabled biological and chemical detection and analysis | |
| Sánchez-Purrà et al. | Reporter selection for nanotags in multiplexed surface enhanced Raman spectroscopy assays | |
| Lin et al. | On‐line SERS detection of single bacterium using novel SERS nanoprobes and a microfluidic dielectrophoresis device | |
| Cao et al. | Raman dye-labeled nanoparticle probes for proteins | |
| Svedhem et al. | Patterns of DNA‐Labeled and scFv‐Antibody‐Carrying Lipid Vesicles Directed by Material‐Specific Immobilization of DNA and Supported Lipid Bilayer Formation on an Au/SiO2 Template | |
| Wilson | The use of gold nanoparticles in diagnostics and detection | |
| Ebrahimi et al. | Nucleic acid based fluorescent nanothermometers | |
| Wang et al. | Detection of the potential pancreatic cancer marker MUC4 in serum using surface-enhanced Raman scattering | |
| Geho et al. | Pegylated, steptavidin-conjugated quantum dots are effective detection elements for reverse-phase protein microarrays | |
| Larmour et al. | Surface enhanced optical spectroscopies for bioanalysis | |
| Wang et al. | Duplex microfluidic SERS detection of pathogen antigens with nanoyeast single-chain variable fragments | |
| JP5860922B2 (ja) | ビーズまたは他の捕捉物を用いた分子または粒子の超高感度検出 | |
| Ko et al. | SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres | |
| US8158340B2 (en) | Methods for detecting conformational changes in bioentities | |
| Tang et al. | New surface-enhanced Raman sensing chip designed for on-site detection of active ricin in complex matrices based on specific depurination | |
| Yao et al. | Detection of femtomolar proteins by nonfluorescent ZnS nanocrystal clusters | |
| Kuo et al. | Sensitive oligonucleotide detection using resonant coupling between fano resonance and image dipoles of gold nanoparticles | |
| Hinman et al. | DNA linkers and diluents for ultrastable gold nanoparticle bioconjugates in multiplexed assay development | |
| Huang et al. | Rapid real-time electrical detection of proteins using single conducting polymer nanowire-based microfluidic aptasensor | |
| Mata Calidonio et al. | Nanomaterial and interface advances in immunoassay biosensors | |
| Oropesa-Nuñez et al. | Insights into the formation of DNA–magnetic nanoparticle hybrid structures: correlations between morphological characterization and output from magnetic biosensor measurements | |
| Hartman et al. | Supported lipid bilayers as dynamic platforms for tethered particles | |
| Ranzoni et al. | Improved immunoassay sensitivity in serum as a result of polymer-entrapped quantum dots:‘papaya particles’ | |
| Shin et al. | Use of multiple peptide-based SERS probes binding to different epitopes on a protein biomarker to improve detection sensitivity |