ES2366061T3 - Un vector y una línea celular de expresión novedosos para la producción en masa de una proteína recombinante y un proceso de producción de proteína recombinante usando los mismos. - Google Patents

Un vector y una línea celular de expresión novedosos para la producción en masa de una proteína recombinante y un proceso de producción de proteína recombinante usando los mismos. Download PDF

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Publication number
ES2366061T3
ES2366061T3 ES07833373T ES07833373T ES2366061T3 ES 2366061 T3 ES2366061 T3 ES 2366061T3 ES 07833373 T ES07833373 T ES 07833373T ES 07833373 T ES07833373 T ES 07833373T ES 2366061 T3 ES2366061 T3 ES 2366061T3
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Prior art keywords
cell line
dhfr
expression vector
gene
expression
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Active
Application number
ES07833373T
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English (en)
Spanish (es)
Inventor
In Young Choi
Chang Hwan Kim
Hyun Ji Lee
Seong Hee Park
Se Chang Kwon
Gwan Sun Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hanmi Pharmaceutical Co Ltd
Hanmi Holdings Co Ltd
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Hanmi Pharmaceutical Co Ltd
Hanmi Holdings Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
ES07833373T 2006-10-16 2007-10-16 Un vector y una línea celular de expresión novedosos para la producción en masa de una proteína recombinante y un proceso de producción de proteína recombinante usando los mismos. Active ES2366061T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20060100507 2006-10-16
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법

Publications (1)

Publication Number Publication Date
ES2366061T3 true ES2366061T3 (es) 2011-10-14

Family

ID=39314214

Family Applications (1)

Application Number Title Priority Date Filing Date
ES07833373T Active ES2366061T3 (es) 2006-10-16 2007-10-16 Un vector y una línea celular de expresión novedosos para la producción en masa de una proteína recombinante y un proceso de producción de proteína recombinante usando los mismos.

Country Status (8)

Country Link
US (1) US8957196B2 (cg-RX-API-DMAC7.html)
EP (1) EP2087118B1 (cg-RX-API-DMAC7.html)
JP (1) JP5076084B2 (cg-RX-API-DMAC7.html)
KR (1) KR100880509B1 (cg-RX-API-DMAC7.html)
CN (1) CN101605899B (cg-RX-API-DMAC7.html)
AT (1) ATE512229T1 (cg-RX-API-DMAC7.html)
ES (1) ES2366061T3 (cg-RX-API-DMAC7.html)
WO (1) WO2008048037A1 (cg-RX-API-DMAC7.html)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030978B1 (ko) * 2008-07-10 2011-04-28 (주) 에이프로젠 동물세포용 재조합 발현벡터
KR101681513B1 (ko) * 2010-02-04 2016-12-02 한미사이언스 주식회사 인간 난포자극 호르몬의 대량 생산 방법
EP2633058B1 (en) * 2010-10-26 2016-07-20 Hanmi Science Co., Ltd. Method for mass production of factor vii/viia
ES2710356T3 (es) 2011-06-17 2019-04-24 Hanmi Science Co Ltd Conjugado que comprende oxintomodulina y un fragmento de inmunoglobulina, y uso del mismo
AR088267A1 (es) 2011-10-06 2014-05-21 Hanmi Science Co Ltd Derivados, de los factores de coagulacion sanguinea vii y viia, conjugados y complejos que comprenden a los mismos y sus usos
EP2809680B1 (en) * 2012-01-31 2016-06-15 Canvax Biotech, S.L. Gpcr with improved cell suface expression
TWI641382B (zh) 2013-01-31 2018-11-21 韓美藥品股份有限公司 於包含因子ｖｉｉ之組成物中使病毒失活之方法
KR20150026024A (ko) * 2013-08-30 2015-03-11 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
EP3059319A4 (en) * 2013-10-07 2017-04-26 Prestige Biopharma Pte. Ltd. Bicistronic expression vector for antibody expression and method for producing antibody using same
RU2548806C1 (ru) * 2014-01-20 2015-04-20 Общество с ограниченной ответственностью "РД-БИОТЕХ" Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина
CN105916883B (zh) 2014-01-28 2019-12-03 北京韩美药品有限公司 双功能融合蛋白及其制备方法和用途
KR102418477B1 (ko) 2014-12-30 2022-07-08 한미약품 주식회사 글루카곤 유도체
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
CN108753819B (zh) * 2018-05-31 2022-05-20 暨南大学 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白
US20240301068A1 (en) 2021-01-08 2024-09-12 Beijing Hanmi Pharmaceutical Co., Ltd. Antibody specifically binding with pd-l1 and antigen-binding fragment of antibody
US20250277044A1 (en) 2021-01-08 2025-09-04 Beijing Hanmi Pharmaceutical Co., Ltd. Antibody specifically binding to 4-1bb and antigen-binding fragment of antibody
EP4276115A4 (en) 2021-01-08 2024-12-18 Beijing Hanmi Pharmaceutical Co., Ltd. BISPECIFIC ANTIBODY IN HETERODIMERIC FORM SIMILAR TO A NATURAL ANTI-PD-1/ANTI-CD47 ANTIBODY STRUCTURE AND PRODUCTION THEREOF

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
KR0162021B1 (ko) * 1995-07-27 1998-11-16 김은영 에리트로포이에틴의 제조방법
KR100232640B1 (ko) * 1997-06-03 1999-12-01 박호군 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
PT986644E (pt) * 1997-07-23 2007-01-31 Roche Diagnostics Gmbh Preparação de eritropoietina por activação genética endógena com promotores virais

Also Published As

Publication number Publication date
EP2087118B1 (en) 2011-06-08
CN101605899A (zh) 2009-12-16
US20100120089A1 (en) 2010-05-13
WO2008048037A1 (en) 2008-04-24
EP2087118A4 (en) 2010-03-03
KR20080034364A (ko) 2008-04-21
EP2087118A1 (en) 2009-08-12
KR100880509B1 (ko) 2009-01-28
HK1137481A1 (en) 2010-07-30
US8957196B2 (en) 2015-02-17
JP2010506586A (ja) 2010-03-04
ATE512229T1 (de) 2011-06-15
JP5076084B2 (ja) 2012-11-21
CN101605899B (zh) 2013-07-31

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