ATE512229T1 - Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit - Google Patents

Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit

Info

Publication number
ATE512229T1
ATE512229T1 AT07833373T AT07833373T ATE512229T1 AT E512229 T1 ATE512229 T1 AT E512229T1 AT 07833373 T AT07833373 T AT 07833373T AT 07833373 T AT07833373 T AT 07833373T AT E512229 T1 ATE512229 T1 AT E512229T1
Authority
AT
Austria
Prior art keywords
recombinant protein
cell line
production
new vector
expression cell
Prior art date
Application number
AT07833373T
Other languages
English (en)
Inventor
In Young Choi
Chang Hwan Kim
Hyun Ji Lee
Seong Hee Park
Se Chang Kwon
Gwan Sun Lee
Original Assignee
Hanmi Holdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Holdings Co Ltd filed Critical Hanmi Holdings Co Ltd
Application granted granted Critical
Publication of ATE512229T1 publication Critical patent/ATE512229T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT07833373T 2006-10-16 2007-10-16 Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit ATE512229T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법
PCT/KR2007/005064 WO2008048037A1 (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

Publications (1)

Publication Number Publication Date
ATE512229T1 true ATE512229T1 (de) 2011-06-15

Family

ID=39314214

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07833373T ATE512229T1 (de) 2006-10-16 2007-10-16 Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit

Country Status (8)

Country Link
US (1) US8957196B2 (de)
EP (1) EP2087118B1 (de)
JP (1) JP5076084B2 (de)
KR (1) KR100880509B1 (de)
CN (1) CN101605899B (de)
AT (1) ATE512229T1 (de)
ES (1) ES2366061T3 (de)
WO (1) WO2008048037A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030978B1 (ko) * 2008-07-10 2011-04-28 (주) 에이프로젠 동물세포용 재조합 발현벡터
KR101681513B1 (ko) * 2010-02-04 2016-12-02 한미사이언스 주식회사 인간 난포자극 호르몬의 대량 생산 방법
US9243237B2 (en) 2010-10-26 2016-01-26 Hanmi Science Co., Ltd Method for mass production of factor VII/VIIA
HRP20190265T1 (hr) 2011-06-17 2019-04-05 Hanmi Science Co., Ltd. Konjugat koji sadrži oksintomodulin i fragment imunoglobulina i njegova uporaba
CN103974716B (zh) 2011-10-06 2016-08-24 韩美科学株式会社 凝血因子Ⅶ和Ⅶa衍生物、包括其的缀合物和复合体及其用途
WO2013113370A1 (en) * 2012-01-31 2013-08-08 Canvax Biotech S.L Gpcr with improved cell surface expression
TWI641382B (zh) 2013-01-31 2018-11-21 韓美藥品股份有限公司 於包含因子ｖｉｉ之組成物中使病毒失活之方法
KR20150026024A (ko) * 2013-08-30 2015-03-11 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
EP3059319A4 (de) * 2013-10-07 2017-04-26 Prestige Biopharma Pte. Ltd. Bicistronischer expressionsvektor zur antikörperexpression und verfahren zur antikörperherstellung damit
RU2548806C1 (ru) * 2014-01-20 2015-04-20 Общество с ограниченной ответственностью "РД-БИОТЕХ" Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина
EP3101035B1 (de) * 2014-01-28 2021-10-06 Beijing Hanmi Pharmaceutical Co., Ltd. Bifunktionelles fusionsprotein, herstellungsverfahren dafür und verwendung davon
KR102418477B1 (ko) 2014-12-30 2022-07-08 한미약품 주식회사 글루카곤 유도체
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
CN108753819B (zh) * 2018-05-31 2022-05-20 暨南大学 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白
WO2022148414A1 (zh) 2021-01-08 2022-07-14 北京韩美药品有限公司 特异性结合pd-l1的抗体及其抗原结合片段
WO2022148411A1 (zh) 2021-01-08 2022-07-14 北京韩美药品有限公司 抗pd-l1/抗cd47天然抗体结构样异源二聚体形式双特异抗体及其制备
JP7744991B2 (ja) 2021-01-08 2025-09-26 北京韓美薬品有限公司 ４－１ｂｂと特異的に結合する抗体及びその抗原結合フラグメント

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
KR0162021B1 (ko) * 1995-07-27 1998-11-16 김은영 에리트로포이에틴의 제조방법
KR100232640B1 (ko) * 1997-06-03 1999-12-01 박호군 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
KR100641969B1 (ko) * 1997-07-23 2006-11-06 로셰 디아그노스틱스 게엠베하 내인성 유전자 활성화에 의한 에리트로포이에틴의제조방법

Also Published As

Publication number Publication date
CN101605899B (zh) 2013-07-31
CN101605899A (zh) 2009-12-16
WO2008048037A1 (en) 2008-04-24
EP2087118B1 (de) 2011-06-08
JP5076084B2 (ja) 2012-11-21
JP2010506586A (ja) 2010-03-04
KR20080034364A (ko) 2008-04-21
HK1137481A1 (en) 2010-07-30
ES2366061T3 (es) 2011-10-14
US8957196B2 (en) 2015-02-17
KR100880509B1 (ko) 2009-01-28
EP2087118A4 (de) 2010-03-03
EP2087118A1 (de) 2009-08-12
US20100120089A1 (en) 2010-05-13

Similar Documents

Publication Publication Date Title
ATE512229T1 (de) Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit
MX2007002382A (es) Producci??n de anticuerpos anti-amiloides beta.
MX2007002380A (es) Produccion de polipeptidos.
DK1664318T3 (da) L-aminosyre-producerende mikroorganisme og fremgangsmåde til fremstilling af L-aminosyre
MX2007003906A (es) Metodos y composiciones para mejorar la produccion de proteinas recombinantes.
TW200619388A (en) Production of TNFR-lg
DK1208205T3 (da) Fremgangsmåder til frembringelse af L-aminosyrer ved hjælp af en forögelse af cellulært NADPH
EA200801074A1 (ru) Способы получения искусственных гибридных семян
SG169359A1 (en) A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
ATE458064T1 (de) Verfahren zur wirksamen rna-interferenz in säugerzellen
MX2009007246A (es) Plantas con rasgos relacionados con el rendimiento mejorado y un metodo para producirlas.
EA201001881A1 (ru) Пролинспецифичная протеаза из penicillium chrysogenum
EA201070229A1 (ru) Нуклеотидные последовательности msca1, влияющие на мужскую фертильность растений, и способ их применения
ATE512228T1 (de) Induzierbare expressionssysteme
DK1573058T3 (da) Fremgangsmåde til forbedring af proteinproduktion
TW200638944A (en) Recombinant enterovirus 71 neutralizing antibodies and use thereof
SG158739A1 (en) Aggrecanase molecules
ATE446374T1 (de) Verfahren zur enantioselektiven enzymatischen reduktion von ketoverbindungen
GB2439039A (en) Identification of a sterol acyltransferase gene
MX2010004699A (es) Plantas que tienen rasgos incrementados relacionados con el rendimiento y un metodo para producirlas.
TW200602487A (en) Polypeptides having DNA polymerase activity
ATE553194T1 (de) Verfahren zur dipeptidherstellung
EA200800098A1 (ru) Экспрессионный вектор и способ продуцирования высоких уровней белков
UA86781C2 (ru) Промотор для эпидермис-специфической трансгенной экспрессии в растениях
ATE451457T1 (de) Verfahren zur dipeptidherstellung

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties