ATE512229T1 - Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit - Google Patents

Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit

Info

Publication number
ATE512229T1
ATE512229T1 AT07833373T AT07833373T ATE512229T1 AT E512229 T1 ATE512229 T1 AT E512229T1 AT 07833373 T AT07833373 T AT 07833373T AT 07833373 T AT07833373 T AT 07833373T AT E512229 T1 ATE512229 T1 AT E512229T1
Authority
AT
Austria
Prior art keywords
recombinant protein
cell line
production
new vector
expression cell
Prior art date
Application number
AT07833373T
Other languages
English (en)
Inventor
In Young Choi
Chang Hwan Kim
Hyun Ji Lee
Seong Hee Park
Se Chang Kwon
Gwan Sun Lee
Original Assignee
Hanmi Holdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Holdings Co Ltd filed Critical Hanmi Holdings Co Ltd
Application granted granted Critical
Publication of ATE512229T1 publication Critical patent/ATE512229T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AT07833373T 2006-10-16 2007-10-16 Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit ATE512229T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법
PCT/KR2007/005064 WO2008048037A1 (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

Publications (1)

Publication Number Publication Date
ATE512229T1 true ATE512229T1 (de) 2011-06-15

Family

ID=39314214

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07833373T ATE512229T1 (de) 2006-10-16 2007-10-16 Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit

Country Status (9)

Country Link
US (1) US8957196B2 (de)
EP (1) EP2087118B1 (de)
JP (1) JP5076084B2 (de)
KR (1) KR100880509B1 (de)
CN (1) CN101605899B (de)
AT (1) ATE512229T1 (de)
ES (1) ES2366061T3 (de)
HK (1) HK1137481A1 (de)
WO (1) WO2008048037A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030978B1 (ko) * 2008-07-10 2011-04-28 (주) 에이프로젠 동물세포용 재조합 발현벡터
KR101681513B1 (ko) * 2010-02-04 2016-12-02 한미사이언스 주식회사 인간 난포자극 호르몬의 대량 생산 방법
WO2012057527A2 (en) 2010-10-26 2012-05-03 Hanmi Holdings Co., Ltd. Method for mass production of factor vii/viia
RU2607365C2 (ru) 2011-06-17 2017-01-10 Ханми Сайенс Ко., Лтд. Конъюгат, содержащий оксинтомодулин и фрагмент иммуноглобулина, и его применение
CN103974716B (zh) 2011-10-06 2016-08-24 韩美科学株式会社 凝血因子Ⅶ和Ⅶa衍生物、包括其的缀合物和复合体及其用途
EP2809680B1 (de) 2012-01-31 2016-06-15 Canvax Biotech, S.L. Gpcr mit verbesserter expression an der zelloberfläche
TWI641382B (zh) 2013-01-31 2018-11-21 韓美藥品股份有限公司 於包含因子ｖｉｉ之組成物中使病毒失活之方法
KR20150026024A (ko) * 2013-08-30 2015-03-11 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
EP3683314A1 (de) * 2013-10-07 2020-07-22 Prestige Biopharma Pte. Ltd. Bicistronischer expressionsvektor zur antikörperexpression und verfahren zur antikörperherstellung damit
RU2548806C1 (ru) * 2014-01-20 2015-04-20 Общество с ограниченной ответственностью "РД-БИОТЕХ" Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина
EP3101035B1 (de) 2014-01-28 2021-10-06 Beijing Hanmi Pharmaceutical Co., Ltd. Bifunktionelles fusionsprotein, herstellungsverfahren dafür und verwendung davon
KR102418477B1 (ko) 2014-12-30 2022-07-08 한미약품 주식회사 글루카곤 유도체
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
CN108753819B (zh) * 2018-05-31 2022-05-20 暨南大学 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白
JP2024502365A (ja) 2021-01-08 2024-01-18 北京韓美薬品有限公司 抗ｐｄ－ｌ１／抗ｃｄ４７天然抗体構造様ヘテロダイマー二重特異性抗体及びその製造方法
KR20230129483A (ko) 2021-01-08 2023-09-08 베이징 한미 파마슈티컬 컴퍼니 리미티드 Pd-l1과 특이적으로 결합하는 항체 및 그 항원 결합단편
EP4276111A1 (de) 2021-01-08 2023-11-15 Beijing Hanmi Pharmaceutical Co., Ltd. Antikörper mit spezifischer bindung an 4-1bb und antigenbindendes fragment des antikörpers

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
KR0162021B1 (ko) * 1995-07-27 1998-11-16 김은영 에리트로포이에틴의 제조방법
KR100232640B1 (ko) * 1997-06-03 1999-12-01 박호군 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
US6391633B1 (en) * 1997-07-23 2002-05-21 Roche Diagnostics Gmbh Production of erythropoietin by endogenous gene activation

Also Published As

Publication number Publication date
EP2087118A1 (de) 2009-08-12
KR100880509B1 (ko) 2009-01-28
US20100120089A1 (en) 2010-05-13
WO2008048037A1 (en) 2008-04-24
CN101605899B (zh) 2013-07-31
HK1137481A1 (en) 2010-07-30
EP2087118B1 (de) 2011-06-08
EP2087118A4 (de) 2010-03-03
KR20080034364A (ko) 2008-04-21
JP5076084B2 (ja) 2012-11-21
CN101605899A (zh) 2009-12-16
US8957196B2 (en) 2015-02-17
JP2010506586A (ja) 2010-03-04
ES2366061T3 (es) 2011-10-14

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