ATE512229T1 - Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit - Google Patents
Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damitInfo
- Publication number
- ATE512229T1 ATE512229T1 AT07833373T AT07833373T ATE512229T1 AT E512229 T1 ATE512229 T1 AT E512229T1 AT 07833373 T AT07833373 T AT 07833373T AT 07833373 T AT07833373 T AT 07833373T AT E512229 T1 ATE512229 T1 AT E512229T1
- Authority
- AT
- Austria
- Prior art keywords
- recombinant protein
- cell line
- production
- new vector
- expression cell
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020060100507A KR100880509B1 (ko) | 2006-10-16 | 2006-10-16 | 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법 |
PCT/KR2007/005064 WO2008048037A1 (en) | 2006-10-16 | 2007-10-16 | A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE512229T1 true ATE512229T1 (de) | 2011-06-15 |
Family
ID=39314214
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07833373T ATE512229T1 (de) | 2006-10-16 | 2007-10-16 | Neuer vektor und expressionszelllinie zur massenproduktion von rekombinantem protein sowie verfahren zur produktion von rekombinantem protein damit |
Country Status (9)
Country | Link |
---|---|
US (1) | US8957196B2 (de) |
EP (1) | EP2087118B1 (de) |
JP (1) | JP5076084B2 (de) |
KR (1) | KR100880509B1 (de) |
CN (1) | CN101605899B (de) |
AT (1) | ATE512229T1 (de) |
ES (1) | ES2366061T3 (de) |
HK (1) | HK1137481A1 (de) |
WO (1) | WO2008048037A1 (de) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101030978B1 (ko) * | 2008-07-10 | 2011-04-28 | (주) 에이프로젠 | 동물세포용 재조합 발현벡터 |
KR101681513B1 (ko) * | 2010-02-04 | 2016-12-02 | 한미사이언스 주식회사 | 인간 난포자극 호르몬의 대량 생산 방법 |
WO2012057527A2 (en) | 2010-10-26 | 2012-05-03 | Hanmi Holdings Co., Ltd. | Method for mass production of factor vii/viia |
RU2607365C2 (ru) | 2011-06-17 | 2017-01-10 | Ханми Сайенс Ко., Лтд. | Конъюгат, содержащий оксинтомодулин и фрагмент иммуноглобулина, и его применение |
CN103974716B (zh) | 2011-10-06 | 2016-08-24 | 韩美科学株式会社 | 凝血因子Ⅶ和Ⅶa衍生物、包括其的缀合物和复合体及其用途 |
EP2809680B1 (de) | 2012-01-31 | 2016-06-15 | Canvax Biotech, S.L. | Gpcr mit verbesserter expression an der zelloberfläche |
TWI641382B (zh) | 2013-01-31 | 2018-11-21 | 韓美藥品股份有限公司 | 於包含因子vii之組成物中使病毒失活之方法 |
KR20150026024A (ko) * | 2013-08-30 | 2015-03-11 | 한미약품 주식회사 | 인간 혈액응고 7인자 유도체의 대량 생산 방법 |
EP3683314A1 (de) * | 2013-10-07 | 2020-07-22 | Prestige Biopharma Pte. Ltd. | Bicistronischer expressionsvektor zur antikörperexpression und verfahren zur antikörperherstellung damit |
RU2548806C1 (ru) * | 2014-01-20 | 2015-04-20 | Общество с ограниченной ответственностью "РД-БИОТЕХ" | Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина |
EP3101035B1 (de) | 2014-01-28 | 2021-10-06 | Beijing Hanmi Pharmaceutical Co., Ltd. | Bifunktionelles fusionsprotein, herstellungsverfahren dafür und verwendung davon |
KR102418477B1 (ko) | 2014-12-30 | 2022-07-08 | 한미약품 주식회사 | 글루카곤 유도체 |
CN106496302B (zh) * | 2015-09-08 | 2021-12-10 | 三生国健药业(上海)股份有限公司 | 一种用离子交换层析纯化蛋白质的方法 |
CN108753819B (zh) * | 2018-05-31 | 2022-05-20 | 暨南大学 | 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白 |
JP2024502365A (ja) | 2021-01-08 | 2024-01-18 | 北京韓美薬品有限公司 | 抗pd-l1/抗cd47天然抗体構造様ヘテロダイマー二重特異性抗体及びその製造方法 |
KR20230129483A (ko) | 2021-01-08 | 2023-09-08 | 베이징 한미 파마슈티컬 컴퍼니 리미티드 | Pd-l1과 특이적으로 결합하는 항체 및 그 항원 결합단편 |
EP4276111A1 (de) | 2021-01-08 | 2023-11-15 | Beijing Hanmi Pharmaceutical Co., Ltd. | Antikörper mit spezifischer bindung an 4-1bb und antigenbindendes fragment des antikörpers |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4399216A (en) | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
KR0162021B1 (ko) * | 1995-07-27 | 1998-11-16 | 김은영 | 에리트로포이에틴의 제조방법 |
KR100232640B1 (ko) * | 1997-06-03 | 1999-12-01 | 박호군 | 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법 |
US6391633B1 (en) * | 1997-07-23 | 2002-05-21 | Roche Diagnostics Gmbh | Production of erythropoietin by endogenous gene activation |
-
2006
- 2006-10-16 KR KR1020060100507A patent/KR100880509B1/ko active IP Right Grant
-
2007
- 2007-10-16 ES ES07833373T patent/ES2366061T3/es active Active
- 2007-10-16 JP JP2009533241A patent/JP5076084B2/ja not_active Expired - Fee Related
- 2007-10-16 AT AT07833373T patent/ATE512229T1/de not_active IP Right Cessation
- 2007-10-16 WO PCT/KR2007/005064 patent/WO2008048037A1/en active Application Filing
- 2007-10-16 EP EP07833373A patent/EP2087118B1/de not_active Not-in-force
- 2007-10-16 US US12/444,567 patent/US8957196B2/en not_active Expired - Fee Related
- 2007-10-16 CN CN2007800384033A patent/CN101605899B/zh not_active Expired - Fee Related
-
2010
- 2010-02-04 HK HK10101256.5A patent/HK1137481A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP2087118A1 (de) | 2009-08-12 |
KR100880509B1 (ko) | 2009-01-28 |
US20100120089A1 (en) | 2010-05-13 |
WO2008048037A1 (en) | 2008-04-24 |
CN101605899B (zh) | 2013-07-31 |
HK1137481A1 (en) | 2010-07-30 |
EP2087118B1 (de) | 2011-06-08 |
EP2087118A4 (de) | 2010-03-03 |
KR20080034364A (ko) | 2008-04-21 |
JP5076084B2 (ja) | 2012-11-21 |
CN101605899A (zh) | 2009-12-16 |
US8957196B2 (en) | 2015-02-17 |
JP2010506586A (ja) | 2010-03-04 |
ES2366061T3 (es) | 2011-10-14 |
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Legal Events
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |