ES2322841T3 - Aislamiento y purificacion magnetica de acidos nucleicos. - Google Patents
Aislamiento y purificacion magnetica de acidos nucleicos. Download PDFInfo
- Publication number
- ES2322841T3 ES2322841T3 ES01977661T ES01977661T ES2322841T3 ES 2322841 T3 ES2322841 T3 ES 2322841T3 ES 01977661 T ES01977661 T ES 01977661T ES 01977661 T ES01977661 T ES 01977661T ES 2322841 T3 ES2322841 T3 ES 2322841T3
- Authority
- ES
- Spain
- Prior art keywords
- cellulose
- magnetizable
- nucleic acids
- dna
- particles
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 81
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 81
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 81
- 238000000746 purification Methods 0.000 title claims abstract description 7
- 230000005291 magnetic effect Effects 0.000 title claims description 25
- 238000009413 insulation Methods 0.000 title description 5
- 239000001913 cellulose Substances 0.000 claims abstract description 133
- 229920002678 cellulose Polymers 0.000 claims abstract description 133
- 239000002245 particle Substances 0.000 claims abstract description 76
- 238000000034 method Methods 0.000 claims abstract description 75
- 150000003839 salts Chemical class 0.000 claims abstract description 32
- 238000002955 isolation Methods 0.000 claims abstract description 24
- 229920001515 polyalkylene glycol Polymers 0.000 claims abstract description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 26
- 239000011534 wash buffer Substances 0.000 claims description 24
- 229920001223 polyethylene glycol Polymers 0.000 claims description 21
- 239000011780 sodium chloride Substances 0.000 claims description 21
- 239000000463 material Substances 0.000 claims description 19
- 239000002202 Polyethylene glycol Substances 0.000 claims description 18
- 239000012149 elution buffer Substances 0.000 claims description 17
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000002253 acid Substances 0.000 claims description 13
- 230000001580 bacterial effect Effects 0.000 claims description 13
- 150000007513 acids Chemical class 0.000 claims description 9
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 8
- 239000013592 cell lysate Substances 0.000 claims description 6
- 241000282414 Homo sapiens Species 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 4
- VJHCJDRQFCCTHL-UHFFFAOYSA-N acetic acid 2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)=O.OCC(O)C(O)C(O)C(O)C=O VJHCJDRQFCCTHL-UHFFFAOYSA-N 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 230000003612 virological effect Effects 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000006166 lysate Substances 0.000 claims description 3
- 235000013311 vegetables Nutrition 0.000 claims description 3
- 229910000480 nickel oxide Inorganic materials 0.000 claims description 2
- GNRSAWUEBMWBQH-UHFFFAOYSA-N oxonickel Chemical compound [Ni]=O GNRSAWUEBMWBQH-UHFFFAOYSA-N 0.000 claims description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims 2
- 239000012535 impurity Substances 0.000 claims 1
- 229910052759 nickel Inorganic materials 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 106
- 239000012148 binding buffer Substances 0.000 description 18
- 239000000203 mixture Substances 0.000 description 17
- 239000013612 plasmid Substances 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 16
- 239000006228 supernatant Substances 0.000 description 16
- 239000000872 buffer Substances 0.000 description 15
- 229920002594 Polyethylene Glycol 8000 Polymers 0.000 description 12
- 210000004369 blood Anatomy 0.000 description 12
- 239000008280 blood Substances 0.000 description 12
- 210000001519 tissue Anatomy 0.000 description 12
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 11
- 238000005406 washing Methods 0.000 description 10
- 239000012139 lysis buffer Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 108010067770 Endopeptidase K Proteins 0.000 description 8
- 238000002156 mixing Methods 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 239000008367 deionised water Substances 0.000 description 7
- 229910021641 deionized water Inorganic materials 0.000 description 7
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 6
- 239000007983 Tris buffer Substances 0.000 description 6
- 238000010828 elution Methods 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical group OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- 238000007399 DNA isolation Methods 0.000 description 5
- 210000004690 animal fin Anatomy 0.000 description 5
- 210000004748 cultured cell Anatomy 0.000 description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 238000000246 agarose gel electrophoresis Methods 0.000 description 4
- 239000001110 calcium chloride Substances 0.000 description 4
- 229910001628 calcium chloride Inorganic materials 0.000 description 4
- 235000011148 calcium chloride Nutrition 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 239000002953 phosphate buffered saline Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 3
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 229920001281 polyalkylene Polymers 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 210000001541 thymus gland Anatomy 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 229960004198 guanidine Drugs 0.000 description 2
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
- 239000000383 hazardous chemical Substances 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- -1 CsCl2 Chemical compound 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 238000011530 RNeasy Mini Kit Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 229910001626 barium chloride Inorganic materials 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 229930013686 lignan Natural products 0.000 description 1
- 150000005692 lignans Chemical class 0.000 description 1
- 235000009408 lignans Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26972901P | 2001-02-16 | 2001-02-16 | |
| US269729P | 2001-02-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2322841T3 true ES2322841T3 (es) | 2009-06-30 |
Family
ID=23028437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES01977661T Expired - Lifetime ES2322841T3 (es) | 2001-02-16 | 2001-10-05 | Aislamiento y purificacion magnetica de acidos nucleicos. |
Country Status (8)
| Country | Link |
|---|---|
| EP (3) | EP2090655B1 (https=) |
| JP (1) | JP4399164B2 (https=) |
| AT (1) | ATE422542T1 (https=) |
| AU (1) | AU2001296762B2 (https=) |
| CA (1) | CA2438066A1 (https=) |
| DE (1) | DE60137652D1 (https=) |
| ES (1) | ES2322841T3 (https=) |
| WO (1) | WO2002066993A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US6855499B1 (en) | 2001-02-16 | 2005-02-15 | Cortex Biochem, Inc. | Magnetic isolation and purification of nucleic acids |
| EP1442045B1 (en) * | 2001-11-06 | 2009-01-07 | Cortex Biochem Inc. | Isolation and purification of nucleic acids |
| JP3983125B2 (ja) | 2002-07-19 | 2007-09-26 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| EP1510577A1 (en) * | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| WO2005026347A1 (en) | 2003-09-09 | 2005-03-24 | Fuji Photo Film Co., Ltd. | Method for isolating and purifying a nucleic acid |
| JP4284250B2 (ja) * | 2003-09-09 | 2009-06-24 | 富士フイルム株式会社 | 核酸分離精製方法 |
| US20050106602A1 (en) | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Simplified methods for isolating nucleic acids from cellular materials |
| US8426126B2 (en) | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| DE102007009347B4 (de) | 2007-02-27 | 2009-11-26 | Agowa Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| EP2157181A1 (de) | 2008-08-13 | 2010-02-24 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Verfahren zur Isolation von Nukleinsäuren und Testkit |
| TWI419974B (zh) | 2008-10-27 | 2013-12-21 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
| WO2010127228A1 (en) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
| CA2787924A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013517803A (ja) | 2010-01-29 | 2013-05-20 | キアジェン ゲイサーズバーグ インコーポレイテッド | サンプル中のｈｐｖの存在を決定および確認する方法 |
| EP2572001A2 (en) | 2010-05-19 | 2013-03-27 | QIAGEN Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| WO2012116220A2 (en) | 2011-02-24 | 2012-08-30 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acid |
| DE102012012523B4 (de) | 2012-06-26 | 2015-02-12 | Magnamedics Gmbh | Reinigung von Nukleinsäuren |
| EP2976424B1 (en) | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisation of biological samples |
| EP3077072B1 (en) | 2013-12-02 | 2019-03-06 | Biocartis N.V. | Extraction of circulating nucleic acids |
| EP3218480A1 (en) * | 2014-11-14 | 2017-09-20 | Corning Incorporated | Methods and kits for post-ivt rna purification |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8507706D0 (en) * | 1985-03-25 | 1985-05-01 | Genetics Int Inc | Magnetic nucleic acid sequences |
| US4921805A (en) * | 1987-07-29 | 1990-05-01 | Life Technologies, Inc. | Nucleic acid capture method |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| ATE167302T1 (de) * | 1989-08-04 | 1998-06-15 | Behringwerke Ag | Heterogene bindungstests |
| GB9003253D0 (en) | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
2001
- 2001-10-05 JP JP2002566667A patent/JP4399164B2/ja not_active Expired - Lifetime
- 2001-10-05 EP EP08021881.1A patent/EP2090655B1/en not_active Expired - Lifetime
- 2001-10-05 CA CA002438066A patent/CA2438066A1/en not_active Abandoned
- 2001-10-05 AT AT01977661T patent/ATE422542T1/de not_active IP Right Cessation
- 2001-10-05 DE DE60137652T patent/DE60137652D1/de not_active Expired - Lifetime
- 2001-10-05 AU AU2001296762A patent/AU2001296762B2/en not_active Ceased
- 2001-10-05 EP EP01977661A patent/EP1368629B1/en not_active Expired - Lifetime
- 2001-10-05 ES ES01977661T patent/ES2322841T3/es not_active Expired - Lifetime
- 2001-10-05 WO PCT/US2001/031637 patent/WO2002066993A1/en not_active Ceased
- 2001-10-05 EP EP10176947.9A patent/EP2363476B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002066993A1 (en) | 2002-08-29 |
| DE60137652D1 (de) | 2009-03-26 |
| EP1368629A1 (en) | 2003-12-10 |
| CA2438066A1 (en) | 2002-08-29 |
| ATE422542T1 (de) | 2009-02-15 |
| EP2363476A1 (en) | 2011-09-07 |
| EP1368629A4 (en) | 2005-07-06 |
| EP2090655A1 (en) | 2009-08-19 |
| JP4399164B2 (ja) | 2010-01-13 |
| EP2363476B1 (en) | 2013-05-29 |
| EP1368629B1 (en) | 2009-02-11 |
| JP2004523238A (ja) | 2004-08-05 |
| AU2001296762B2 (en) | 2007-03-15 |
| EP2090655B1 (en) | 2013-05-08 |
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