ATE422542T1 - Magnetische isolierung und reinigung von nukleinsäuren - Google Patents
Magnetische isolierung und reinigung von nukleinsäurenInfo
- Publication number
- ATE422542T1 ATE422542T1 AT01977661T AT01977661T ATE422542T1 AT E422542 T1 ATE422542 T1 AT E422542T1 AT 01977661 T AT01977661 T AT 01977661T AT 01977661 T AT01977661 T AT 01977661T AT E422542 T1 ATE422542 T1 AT E422542T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acids
- purification
- derivatives
- magnetic isolation
- magnetizable cellulose
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 229920002678 cellulose Polymers 0.000 abstract 3
- 239000001913 cellulose Substances 0.000 abstract 3
- 239000002245 particle Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 1
- 229920001515 polyalkylene glycol Polymers 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26972901P | 2001-02-16 | 2001-02-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE422542T1 true ATE422542T1 (de) | 2009-02-15 |
Family
ID=23028437
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01977661T ATE422542T1 (de) | 2001-02-16 | 2001-10-05 | Magnetische isolierung und reinigung von nukleinsäuren |
Country Status (8)
| Country | Link |
|---|---|
| EP (3) | EP2090655B1 (https=) |
| JP (1) | JP4399164B2 (https=) |
| AT (1) | ATE422542T1 (https=) |
| AU (1) | AU2001296762B2 (https=) |
| CA (1) | CA2438066A1 (https=) |
| DE (1) | DE60137652D1 (https=) |
| ES (1) | ES2322841T3 (https=) |
| WO (1) | WO2002066993A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| US6855499B1 (en) | 2001-02-16 | 2005-02-15 | Cortex Biochem, Inc. | Magnetic isolation and purification of nucleic acids |
| EP1442045B1 (en) * | 2001-11-06 | 2009-01-07 | Cortex Biochem Inc. | Isolation and purification of nucleic acids |
| JP3983125B2 (ja) | 2002-07-19 | 2007-09-26 | 富士フイルム株式会社 | 核酸の分離精製方法 |
| EP1510577A1 (en) * | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| WO2005026347A1 (en) | 2003-09-09 | 2005-03-24 | Fuji Photo Film Co., Ltd. | Method for isolating and purifying a nucleic acid |
| JP4284250B2 (ja) * | 2003-09-09 | 2009-06-24 | 富士フイルム株式会社 | 核酸分離精製方法 |
| US20050106602A1 (en) | 2003-11-17 | 2005-05-19 | Hashem Akhavan-Tafti | Simplified methods for isolating nucleic acids from cellular materials |
| US8426126B2 (en) | 2004-03-18 | 2013-04-23 | Applied Biosystems, Llc | Modified surfaces as solid supports for nucleic acid purification |
| DE102007009347B4 (de) | 2007-02-27 | 2009-11-26 | Agowa Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| EP2157181A1 (de) | 2008-08-13 | 2010-02-24 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Verfahren zur Isolation von Nukleinsäuren und Testkit |
| TWI419974B (zh) | 2008-10-27 | 2013-12-21 | Qiagen Gaithersburg Inc | 於自動平台上之快速結果雜交捕捉法 |
| WO2010127228A1 (en) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
| CA2787924A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013517803A (ja) | 2010-01-29 | 2013-05-20 | キアジェン ゲイサーズバーグ インコーポレイテッド | サンプル中のｈｐｖの存在を決定および確認する方法 |
| EP2572001A2 (en) | 2010-05-19 | 2013-03-27 | QIAGEN Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP6153866B2 (ja) | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| WO2012116220A2 (en) | 2011-02-24 | 2012-08-30 | Qiagen Gaithersburg, Inc. | Materials and methods for detection of hpv nucleic acid |
| DE102012012523B4 (de) | 2012-06-26 | 2015-02-12 | Magnamedics Gmbh | Reinigung von Nukleinsäuren |
| EP2976424B1 (en) | 2013-03-18 | 2018-10-03 | Qiagen GmbH | Stabilisation of biological samples |
| EP3077072B1 (en) | 2013-12-02 | 2019-03-06 | Biocartis N.V. | Extraction of circulating nucleic acids |
| EP3218480A1 (en) * | 2014-11-14 | 2017-09-20 | Corning Incorporated | Methods and kits for post-ivt rna purification |
| US20180135040A1 (en) | 2016-02-16 | 2018-05-17 | Life Magnetics, Inc. | Methods for separating nucleic acids with graphene coated magnetic beads |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8507706D0 (en) * | 1985-03-25 | 1985-05-01 | Genetics Int Inc | Magnetic nucleic acid sequences |
| US4921805A (en) * | 1987-07-29 | 1990-05-01 | Life Technologies, Inc. | Nucleic acid capture method |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| ATE167302T1 (de) * | 1989-08-04 | 1998-06-15 | Behringwerke Ag | Heterogene bindungstests |
| GB9003253D0 (en) | 1990-02-13 | 1990-04-11 | Amersham Int Plc | Precipitating polymers |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
2001
- 2001-10-05 JP JP2002566667A patent/JP4399164B2/ja not_active Expired - Lifetime
- 2001-10-05 EP EP08021881.1A patent/EP2090655B1/en not_active Expired - Lifetime
- 2001-10-05 CA CA002438066A patent/CA2438066A1/en not_active Abandoned
- 2001-10-05 AT AT01977661T patent/ATE422542T1/de not_active IP Right Cessation
- 2001-10-05 DE DE60137652T patent/DE60137652D1/de not_active Expired - Lifetime
- 2001-10-05 AU AU2001296762A patent/AU2001296762B2/en not_active Ceased
- 2001-10-05 EP EP01977661A patent/EP1368629B1/en not_active Expired - Lifetime
- 2001-10-05 ES ES01977661T patent/ES2322841T3/es not_active Expired - Lifetime
- 2001-10-05 WO PCT/US2001/031637 patent/WO2002066993A1/en not_active Ceased
- 2001-10-05 EP EP10176947.9A patent/EP2363476B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2322841T3 (es) | 2009-06-30 |
| WO2002066993A1 (en) | 2002-08-29 |
| DE60137652D1 (de) | 2009-03-26 |
| EP1368629A1 (en) | 2003-12-10 |
| CA2438066A1 (en) | 2002-08-29 |
| EP2363476A1 (en) | 2011-09-07 |
| EP1368629A4 (en) | 2005-07-06 |
| EP2090655A1 (en) | 2009-08-19 |
| JP4399164B2 (ja) | 2010-01-13 |
| EP2363476B1 (en) | 2013-05-29 |
| EP1368629B1 (en) | 2009-02-11 |
| JP2004523238A (ja) | 2004-08-05 |
| AU2001296762B2 (en) | 2007-03-15 |
| EP2090655B1 (en) | 2013-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1368629 Country of ref document: EP |
|
| REN | Ceased due to non-payment of the annual fee |