ES2317706T3 - Metodo para produicir polipeptidos en celulas mutantes de aspergillus. - Google Patents
Metodo para produicir polipeptidos en celulas mutantes de aspergillus. Download PDFInfo
- Publication number
- ES2317706T3 ES2317706T3 ES99960956T ES99960956T ES2317706T3 ES 2317706 T3 ES2317706 T3 ES 2317706T3 ES 99960956 T ES99960956 T ES 99960956T ES 99960956 T ES99960956 T ES 99960956T ES 2317706 T3 ES2317706 T3 ES 2317706T3
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- Prior art keywords
- aspergillus
- toxin
- mutant
- polypeptide
- cell
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/69—Aspergillus oryzae
Landscapes
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- Botany (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA199801726 | 1998-12-23 | ||
| DK199801726 | 1998-12-23 | ||
| DKPA199900745 | 1999-05-27 | ||
| DK199900745 | 1999-05-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2317706T3 true ES2317706T3 (es) | 2009-04-16 |
Family
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES99960956T Expired - Lifetime ES2317706T3 (es) | 1998-12-23 | 1999-12-22 | Metodo para produicir polipeptidos en celulas mutantes de aspergillus. |
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| Country | Link |
|---|---|
| EP (1) | EP1141371B1 (enExample) |
| JP (2) | JP2002533133A (enExample) |
| CN (1) | CN100529096C (enExample) |
| AT (1) | ATE414782T1 (enExample) |
| AU (1) | AU1774000A (enExample) |
| DE (1) | DE69939947D1 (enExample) |
| DK (1) | DK1141371T3 (enExample) |
| ES (1) | ES2317706T3 (enExample) |
| WO (1) | WO2000039322A1 (enExample) |
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| EP2410048B1 (en) | 2005-01-24 | 2016-08-10 | DSM IP Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| EP1739428A1 (en) * | 2005-07-01 | 2007-01-03 | Institut Pasteur | In vitro diagnostic method and kit for an aspergillus infection |
| EP1941025B1 (en) | 2005-10-17 | 2011-06-29 | Novozymes A/S | Use of fungal mutants for expression of antibodies |
| EP2423315B1 (en) | 2006-06-29 | 2015-01-07 | DSM IP Assets B.V. | A method for achieving improved polypeptide expression |
| WO2008008950A2 (en) | 2006-07-14 | 2008-01-17 | Novozymes, Inc. | Methods for producing secreted polypeptides having biological activity |
| WO2009051152A1 (ja) * | 2007-10-19 | 2009-04-23 | Noda Institute For Scientific Research | ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子 |
| CN102292444A (zh) | 2008-11-20 | 2011-12-21 | 诺维信股份有限公司 | 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸 |
| WO2010084086A1 (en) | 2009-01-21 | 2010-07-29 | Novozymes A/S | Polypeptides having esterase activity and nucleic acids encoding the same |
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| CN102414323B (zh) | 2009-04-22 | 2015-07-08 | 帝斯曼知识产权资产管理有限公司 | 用于生产感兴趣的重组多肽的方法 |
| AU2010240861B2 (en) | 2009-04-24 | 2015-02-05 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| CA2768608A1 (en) | 2009-07-22 | 2011-01-27 | Dsm Ip Assets B.V. | Improved host cell for the production of a compound of interest |
| US8586829B2 (en) | 2009-09-30 | 2013-11-19 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| BR112012008260A2 (pt) | 2009-11-06 | 2015-09-15 | Novozymes Inc E Novozymes As | polipeptídeo, polinucleotídeo, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, planta transgênica, parte da planta ou célula da planta,e, molécula de rna inibitória de filamento duplo. |
| WO2011057140A1 (en) | 2009-11-06 | 2011-05-12 | Novozymes, Inc. | Compositions for saccharification of cellulosic material |
| ES2574054T3 (es) | 2009-11-06 | 2016-06-14 | Novozymes, Inc. | Polipéptidos con actividad de xilanasa y polinucleótidos que los codifican |
| US9133448B2 (en) | 2010-02-11 | 2015-09-15 | Dsm Ip Assets B.V. | Polypeptide having cellobiohydrolase activity and uses thereof |
| MX2012009274A (es) | 2010-02-11 | 2012-12-05 | Dsm Ip Assets Bv | Celula hospedera capaz de producir enzimas utiles para la degradacion de material lignocelulosico. |
| US8735563B2 (en) | 2010-06-25 | 2014-05-27 | Novozymes A/S | Polynucleotides having promoter activity |
| WO2011161208A1 (en) | 2010-06-25 | 2011-12-29 | Novozymes A/S | Polynucleotides having promoter activity |
| US9222095B2 (en) | 2010-06-25 | 2015-12-29 | Novozymes A/S | Polynucleotides having leader sequence function |
| EA201300054A1 (ru) | 2010-06-29 | 2013-05-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид, обладающий активностью бета-глюкозидазы, и его применение |
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| MX2012014993A (es) | 2010-06-29 | 2013-02-26 | Dsm Ip Assets Bv | Polipeptido que tiene actividad degradante de carbohidratos y usos de los mismos. |
| BR112012033703A2 (pt) | 2010-06-29 | 2016-10-11 | Dsm Ip Assets Bv | polipeptídeo tendo atividade beta-glicosidase e seus usos |
| EA201300056A1 (ru) | 2010-06-29 | 2013-07-30 | ДСМ АйПи АССЕТС Б.В. | Полипептид с активностью ацетилксиланэстеразы и его применения |
| BR112012033404B1 (pt) | 2010-06-29 | 2021-05-18 | Dsm Ip Assets B.V. | célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo |
| US8581042B2 (en) | 2010-06-30 | 2013-11-12 | Novozymes A/S | Polypeptides having beta-glucosidase activity and polynucleotides encoding same |
| US8945898B2 (en) * | 2010-07-01 | 2015-02-03 | Dsm Ip Assets B.V. | Recombinant host cell with deficiency in non-ribosomal peptide synthase production |
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| WO2012062220A1 (en) | 2010-11-12 | 2012-05-18 | Novozymes A/S | Polypeptides having endoglucanase activity and polynucleotides encoding same |
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| WO2012103350A1 (en) | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides having cellobiohydrolase activity and polynucleotides encoding same |
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| DK2702153T3 (en) | 2011-04-28 | 2019-03-18 | Novozymes Inc | Polypeptides with endoglucanase activity and polynucleotides encoding them |
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| MY208100A (en) | 2017-08-25 | 2025-04-15 | Novozymes As | Enzyme assisted crude palm oil extraction |
| US11667922B2 (en) | 2018-03-26 | 2023-06-06 | Novozymes A/S | Fungal chaperone proteins |
| CN108795903B (zh) * | 2018-06-08 | 2020-12-15 | 南京林业大学 | 一种高酶活淀粉酶的表达方法 |
| CN108823178B (zh) * | 2018-06-08 | 2021-12-10 | 中国中医科学院中药研究所 | 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用 |
| CN108753748B (zh) * | 2018-06-08 | 2021-12-10 | 中国中医科学院中药研究所 | 大黄素糖基转移酶蛋白FtUGT75R2及其编码基因与应用 |
| EP3894551A1 (en) | 2018-12-12 | 2021-10-20 | Novozymes A/S | Polypeptides having xylanase activity and polynucleotides encoding same |
| FI3898985T3 (fi) | 2018-12-21 | 2023-05-05 | Novozymes As | Proteiinien tandemekspressio |
| CN110218750B (zh) * | 2019-04-24 | 2023-04-11 | 郑州轻工业学院 | 杂色曲霉菌株在发酵生产曲酸中应用 |
| CN110106152B (zh) * | 2019-04-30 | 2021-08-17 | 中国农业科学院油料作物研究所 | 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体 |
| CN110108874B (zh) * | 2019-04-30 | 2022-09-20 | 中国农业科学院油料作物研究所 | 快速检测环匹阿尼酸的胶体金免疫分析试纸条、制备及其应用 |
| US20220267783A1 (en) | 2019-07-25 | 2022-08-25 | Novozymes A/S | Filamentous fungal expression system |
| CN111139189B (zh) * | 2020-01-14 | 2022-04-19 | 浙江工业大学 | 曲霉wbx-38及其在生产环匹阿尼酸中的应用 |
| CN116024096B (zh) * | 2022-07-14 | 2025-06-03 | 浙江树人学院 | 泡盛曲霉sr3-28及其在从朱砂七提取大黄素中的应用 |
| AU2023410701A1 (en) | 2022-12-21 | 2025-05-22 | Novo Nordisk Pharmatech A/S | Microbial proteases for cell detachment |
| CN116064437B (zh) * | 2023-02-20 | 2025-09-23 | 武汉轻工大学 | 用于降解黄曲霉毒素的复合酶制剂、复合酶制剂溶液及其制备方法和应用 |
| WO2025133003A2 (en) | 2023-12-19 | 2025-06-26 | 21St.Bio A/S | Non-animal bovine beta lactoglobulin |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE730656T1 (de) * | 1993-12-01 | 1998-04-30 | Novo Nordisk Biotech Inc | Aspergillus poetidus exprimierungssystem |
| AU695766B2 (en) * | 1993-12-01 | 1998-08-20 | Novo Nordisk Biotech, Inc. | (Aspergillus) expression system |
| EP0966521A1 (en) * | 1996-09-13 | 1999-12-29 | Novo Nordisk Biotech, Inc. | Cells having dna insertion mutations which produce altered amounts of a polypeptide |
| ATE313620T1 (de) * | 1996-09-19 | 2006-01-15 | Novozymes As | Wirtszellen und methoden für die produktion von proteinen |
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1999
- 1999-12-22 AT AT99960956T patent/ATE414782T1/de active
- 1999-12-22 JP JP2000591212A patent/JP2002533133A/ja not_active Withdrawn
- 1999-12-22 EP EP99960956A patent/EP1141371B1/en not_active Expired - Lifetime
- 1999-12-22 WO PCT/DK1999/000726 patent/WO2000039322A1/en not_active Ceased
- 1999-12-22 AU AU17740/00A patent/AU1774000A/en not_active Abandoned
- 1999-12-22 DE DE69939947T patent/DE69939947D1/de not_active Expired - Lifetime
- 1999-12-22 ES ES99960956T patent/ES2317706T3/es not_active Expired - Lifetime
- 1999-12-22 DK DK99960956T patent/DK1141371T3/da active
- 1999-12-22 CN CNB99815766XA patent/CN100529096C/zh not_active Expired - Lifetime
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2011
- 2011-05-06 JP JP2011103808A patent/JP5453343B2/ja not_active Expired - Lifetime
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|---|---|
| AU1774000A (en) | 2000-07-31 |
| CN100529096C (zh) | 2009-08-19 |
| EP1141371B1 (en) | 2008-11-19 |
| EP1141371A1 (en) | 2001-10-10 |
| WO2000039322A1 (en) | 2000-07-06 |
| JP2011188863A (ja) | 2011-09-29 |
| JP5453343B2 (ja) | 2014-03-26 |
| JP2002533133A (ja) | 2002-10-08 |
| DE69939947D1 (de) | 2009-01-02 |
| ATE414782T1 (de) | 2008-12-15 |
| CN1333837A (zh) | 2002-01-30 |
| DK1141371T3 (da) | 2009-02-02 |
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