CN100529096C - 在曲霉属突变细胞中产生多肽的方法 - Google Patents

在曲霉属突变细胞中产生多肽的方法 Download PDF

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CN100529096C
CN100529096C CNB99815766XA CN99815766A CN100529096C CN 100529096 C CN100529096 C CN 100529096C CN B99815766X A CNB99815766X A CN B99815766XA CN 99815766 A CN99815766 A CN 99815766A CN 100529096 C CN100529096 C CN 100529096C
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aspergillus
toxin
cell
mutant
polypeptide
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CN1333837A (zh
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比约恩·E·克里斯坦森
亨里克·莫尔加尔德
斯文德·卡斯加尔德
简·莱姆贝克
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Novo Nordisk AS
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae

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CNB99815766XA 1998-12-23 1999-12-22 在曲霉属突变细胞中产生多肽的方法 Expired - Lifetime CN100529096C (zh)

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DKPA199801726 1998-12-23
DKPA199801726 1998-12-23
DKPA199900745 1999-05-27
DKPA199900745 1999-05-27

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CN1333837A CN1333837A (zh) 2002-01-30
CN100529096C true CN100529096C (zh) 2009-08-19

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EP (1) EP1141371B1 (enExample)
JP (2) JP2002533133A (enExample)
CN (1) CN100529096C (enExample)
AT (1) ATE414782T1 (enExample)
AU (1) AU1774000A (enExample)
DE (1) DE69939947D1 (enExample)
DK (1) DK1141371T3 (enExample)
ES (1) ES2317706T3 (enExample)
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Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070957A2 (en) * 2002-02-20 2003-08-28 Novozymes A/S Plant polypeptide production
EP1886582B1 (en) 2002-10-11 2015-01-14 Novozymes A/S Method of preparing a heat-treated product
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
MXPA05011421A (es) 2003-04-28 2006-05-31 Novozymes As Fosfolipasa y metodo para producirla.
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
EP1814996A2 (en) 2004-11-19 2007-08-08 Novozymes A/S Polypeptides having antimicrobial activity and polynucleotides encoding same
DK2365068T3 (en) 2004-12-22 2017-05-15 Novozymes As ENZYMER FOR PROCESSING STARCH
EP2410048B1 (en) 2005-01-24 2016-08-10 DSM IP Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP1739428A1 (en) * 2005-07-01 2007-01-03 Institut Pasteur In vitro diagnostic method and kit for an aspergillus infection
EP1941025B1 (en) 2005-10-17 2011-06-29 Novozymes A/S Use of fungal mutants for expression of antibodies
EP2423315B1 (en) 2006-06-29 2015-01-07 DSM IP Assets B.V. A method for achieving improved polypeptide expression
WO2008008950A2 (en) 2006-07-14 2008-01-17 Novozymes, Inc. Methods for producing secreted polypeptides having biological activity
WO2009051152A1 (ja) * 2007-10-19 2009-04-23 Noda Institute For Scientific Research ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子
CN102292444A (zh) 2008-11-20 2011-12-21 诺维信股份有限公司 具有淀粉分解增强活性的多肽和编码该多肽的多核苷酸
WO2010084086A1 (en) 2009-01-21 2010-07-29 Novozymes A/S Polypeptides having esterase activity and nucleic acids encoding the same
DK2411511T3 (en) 2009-03-24 2018-11-26 Novozymes As POLYPEPTIDES WITH ACETYLXYLANESTERASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
CN102414323B (zh) 2009-04-22 2015-07-08 帝斯曼知识产权资产管理有限公司 用于生产感兴趣的重组多肽的方法
AU2010240861B2 (en) 2009-04-24 2015-02-05 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
CA2768608A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
US8586829B2 (en) 2009-09-30 2013-11-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
BR112012008260A2 (pt) 2009-11-06 2015-09-15 Novozymes Inc E Novozymes As polipeptídeo, polinucleotídeo, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, para inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para produzir um produto de fermentação, para fermentar um material celulósico, planta transgênica, parte da planta ou célula da planta,e, molécula de rna inibitória de filamento duplo.
WO2011057140A1 (en) 2009-11-06 2011-05-12 Novozymes, Inc. Compositions for saccharification of cellulosic material
ES2574054T3 (es) 2009-11-06 2016-06-14 Novozymes, Inc. Polipéptidos con actividad de xilanasa y polinucleótidos que los codifican
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
MX2012009274A (es) 2010-02-11 2012-12-05 Dsm Ip Assets Bv Celula hospedera capaz de producir enzimas utiles para la degradacion de material lignocelulosico.
US8735563B2 (en) 2010-06-25 2014-05-27 Novozymes A/S Polynucleotides having promoter activity
WO2011161208A1 (en) 2010-06-25 2011-12-29 Novozymes A/S Polynucleotides having promoter activity
US9222095B2 (en) 2010-06-25 2015-12-29 Novozymes A/S Polynucleotides having leader sequence function
EA201300054A1 (ru) 2010-06-29 2013-05-30 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий активностью бета-глюкозидазы, и его применение
CN103180337A (zh) 2010-06-29 2013-06-26 帝斯曼知识产权资产管理有限公司 具有膨胀因子活性的多肽及其用途
MX2012014993A (es) 2010-06-29 2013-02-26 Dsm Ip Assets Bv Polipeptido que tiene actividad degradante de carbohidratos y usos de los mismos.
BR112012033703A2 (pt) 2010-06-29 2016-10-11 Dsm Ip Assets Bv polipeptídeo tendo atividade beta-glicosidase e seus usos
EA201300056A1 (ru) 2010-06-29 2013-07-30 ДСМ АйПи АССЕТС Б.В. Полипептид с активностью ацетилксиланэстеразы и его применения
BR112012033404B1 (pt) 2010-06-29 2021-05-18 Dsm Ip Assets B.V. célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo
US8581042B2 (en) 2010-06-30 2013-11-12 Novozymes A/S Polypeptides having beta-glucosidase activity and polynucleotides encoding same
US8945898B2 (en) * 2010-07-01 2015-02-03 Dsm Ip Assets B.V. Recombinant host cell with deficiency in non-ribosomal peptide synthase production
MX2013001540A (es) 2010-08-12 2013-04-24 Novozymes Inc Composiciones que comprende un polipeptido que tiene actividad de incremento celulolititico y un compuesto de quinona, y uso de las mismas.
WO2012030844A1 (en) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
US9187742B2 (en) 2010-08-30 2015-11-17 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2012062220A1 (en) 2010-11-12 2012-05-18 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012103322A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP3235903B1 (en) 2011-01-26 2021-07-07 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK2668266T3 (en) 2011-01-26 2018-03-26 Novozymes Inc POLYPEPTIDES WITH CELLOBIO HYDROASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
BR112013018695B1 (pt) 2011-01-26 2021-03-30 Novozymes A / S Célula hospedeira microbiana recombinante, processos para produzir um polipeptídeo, para produzir uma proteína, para degradar um material celulósico, e, construto de ácido nucleico ou vetor de expressão
WO2012103350A1 (en) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
DK3339442T3 (da) 2011-03-09 2022-06-20 Novozymes Inc Fremgangsmåder til forøgelse af den cellulolyseforbedrende aktivitet af et polypeptid
DK2702153T3 (en) 2011-04-28 2019-03-18 Novozymes Inc Polypeptides with endoglucanase activity and polynucleotides encoding them
CN103797126A (zh) 2011-04-29 2014-05-14 诺维信股份有限公司 用于增强纤维素材料的降解或转化的方法
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
US9663772B2 (en) 2011-08-24 2017-05-30 Novozymes, Inc. Aspergillus fumigatus cellulolytic enzyme compositions and uses thereof
DK2773656T3 (da) 2011-10-31 2019-09-09 Novozymes Inc Polypeptider med cellulolyseforbedrende aktivitet og polynukleotider, som koder for dem
EP3219794B1 (en) 2011-11-21 2019-09-11 Novozymes A/S Gh61 polypeptide variants and polynucleotides encoding same
CN113234695A (zh) 2012-04-27 2021-08-10 诺维信股份有限公司 Gh61多肽变体以及编码其的多核苷酸
AU2013311668B2 (en) 2012-09-05 2019-02-28 Novozymes A/S Polypeptides having protease activity
WO2014092832A2 (en) 2012-09-19 2014-06-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
WO2014058896A1 (en) 2012-10-08 2014-04-17 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
WO2014093835A1 (en) 2012-12-14 2014-06-19 Novozymes A/S Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
US9556465B2 (en) 2013-05-10 2017-01-31 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
WO2016037096A1 (en) 2014-09-05 2016-03-10 Novozymes A/S Carbohydrate binding module variants and polynucleotides encoding same
AU2015366380B2 (en) 2014-12-19 2021-07-22 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
DK3262165T3 (da) 2015-02-24 2020-08-24 Novozymes As Cellobiohydrolasevarianter og polynukleotider, som koder for dem
US10519520B2 (en) 2015-05-27 2019-12-31 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
EP3353195B1 (en) 2015-09-22 2021-11-10 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
SG11201911802TA (en) * 2017-06-07 2020-01-30 Ptt Global Chemical Public Co Ltd Mutant strain aspergillus aculeatus for producing cellulase and xylanase and preparation method thereof
MY208100A (en) 2017-08-25 2025-04-15 Novozymes As Enzyme assisted crude palm oil extraction
US11667922B2 (en) 2018-03-26 2023-06-06 Novozymes A/S Fungal chaperone proteins
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EP3894551A1 (en) 2018-12-12 2021-10-20 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
FI3898985T3 (fi) 2018-12-21 2023-05-05 Novozymes As Proteiinien tandemekspressio
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015391A2 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus expression system
WO1995015390A1 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus foetidus expression system

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0966521A1 (en) * 1996-09-13 1999-12-29 Novo Nordisk Biotech, Inc. Cells having dna insertion mutations which produce altered amounts of a polypeptide
ATE313620T1 (de) * 1996-09-19 2006-01-15 Novozymes As Wirtszellen und methoden für die produktion von proteinen

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015391A2 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus expression system
WO1995015390A1 (en) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. Aspergillus foetidus expression system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
杂色曲霉毒素研究进展. 赵宝玉，曹光荣.动物医学进展，第18卷第2期. 1997 *

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EP1141371A1 (en) 2001-10-10
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JP5453343B2 (ja) 2014-03-26
JP2002533133A (ja) 2002-10-08
DE69939947D1 (de) 2009-01-02
ATE414782T1 (de) 2008-12-15
CN1333837A (zh) 2002-01-30
DK1141371T3 (da) 2009-02-02
ES2317706T3 (es) 2009-04-16

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