ES2311638T5 - Métodos de uso de polimerasas mejoradas. - Google Patents
Métodos de uso de polimerasas mejoradas. Download PDFInfo
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- ES2311638T5 ES2311638T5 ES02786844T ES02786844T ES2311638T5 ES 2311638 T5 ES2311638 T5 ES 2311638T5 ES 02786844 T ES02786844 T ES 02786844T ES 02786844 T ES02786844 T ES 02786844T ES 2311638 T5 ES2311638 T5 ES 2311638T5
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
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- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
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| US33396601P | 2001-11-28 | 2001-11-28 | |
| US333966P | 2001-11-28 |
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| ES2311638T3 ES2311638T3 (es) | 2009-02-16 |
| ES2311638T5 true ES2311638T5 (es) | 2011-09-08 |
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| ES02786844T Expired - Lifetime ES2311638T5 (es) | 2001-11-28 | 2002-11-27 | Métodos de uso de polimerasas mejoradas. |
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| EP (1) | EP1456394B2 (enExample) |
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| DK (1) | DK1456394T4 (enExample) |
| ES (1) | ES2311638T5 (enExample) |
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| US7820808B2 (en) | 2003-04-01 | 2010-10-26 | Eragen Biosciences, Inc. | Polymerase inhibitor and method of using same |
| EP1706502A4 (en) * | 2003-11-26 | 2007-05-23 | Eppendorf Ag | METHODS AND COMPOSITIONS FOR IN VITRO AMPLIFICATION OF EXTRACHROMOSOMIC NUCLEIC ACID |
| WO2006074288A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity |
| WO2006074233A2 (en) * | 2005-01-06 | 2006-07-13 | Applera Corporation | Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification |
| WO2007050125A2 (en) * | 2005-05-27 | 2007-05-03 | William Marsh Rice University | High processivity polymerases |
| US20070059713A1 (en) * | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
| JP4899618B2 (ja) * | 2006-05-01 | 2012-03-21 | ソニー株式会社 | 二本鎖核酸検出方法、ハイブリダイゼーション検出方法 |
| KR100825279B1 (ko) * | 2006-11-30 | 2008-04-25 | 한국해양연구원 | Ｄｎα 중합효소 활성 증가 단백질 및 이를 암호화 하는유전자 |
| EP3029146B1 (en) * | 2007-11-27 | 2018-08-01 | Bio-rad Laboratories, Inc. | Reduced inhibition of one-step rt-pcr |
| EP2240576B1 (en) * | 2008-01-11 | 2012-10-03 | Genesys Ltd | Cren7 chimeric protein |
| GB0803628D0 (en) | 2008-02-28 | 2008-04-02 | Genesys Ltd | Enzyme |
| EP3101124B1 (en) | 2008-11-03 | 2019-06-19 | Kapa Biosystems, Inc. | Modified type a dna polymerases |
| JP5818688B2 (ja) | 2009-01-08 | 2015-11-18 | バイオ−ラッド ラボラトリーズ インコーポレーティッド | 核酸増幅反応の効率を改善するための方法および組成物 |
| US20130022980A1 (en) * | 2009-02-04 | 2013-01-24 | Lucigen Corporation | Rna- and dna-copying enzymes |
| US9617588B2 (en) | 2010-10-05 | 2017-04-11 | Thermo Fisher Scientific Baltics Uab | Enzyme mixture |
| WO2012097318A2 (en) | 2011-01-14 | 2012-07-19 | Kap Abiosystems | Modified dna polymerases for improved amplification |
| EP2694670B1 (en) | 2011-04-08 | 2017-07-19 | Bio-Rad Laboratories, Inc. | Pcr reaction mixtures with decreased non-specific activity |
| US8697410B2 (en) | 2011-04-08 | 2014-04-15 | Bio-Rad Laboratories, Inc. | SSO7-polymerase conjugates with decreased non-specific activity |
| US8470573B2 (en) * | 2011-06-21 | 2013-06-25 | Bio-Rad Laboratories, Inc. | Hybrid polymerases having the ability to produce long amplicons |
| US10023856B2 (en) * | 2013-09-25 | 2018-07-17 | Thermo Fisher Scientific Baltics Uab | Enzyme composition for DNA end repair, adenylation, phosphorylation |
| WO2016028671A1 (en) | 2014-08-19 | 2016-02-25 | Bio-Rad Laboratories, Inc. | Rna amplification methods |
| US9963687B2 (en) * | 2014-08-27 | 2018-05-08 | New England Biolabs, Inc. | Fusion polymerase and method for using the same |
| EP3450558B1 (en) * | 2014-08-27 | 2020-09-30 | New England Biolabs, Inc. | Synthon formation |
| EP3259373B1 (en) | 2015-02-17 | 2022-07-27 | Bio-Rad Laboratories, Inc. | Small nucleic acid quantification using split cycle amplification |
| CN111556900A (zh) | 2017-12-22 | 2020-08-18 | 赛默飞世尔科技波罗的海封闭股份公司 | 包括胺的聚合酶链反应组合物 |
| CN116200364A (zh) * | 2019-03-10 | 2023-06-02 | 武汉爱博泰克生物科技有限公司 | 用于在提高的盐浓度或体液中扩增的突变型Taq聚合酶 |
| US11613738B2 (en) * | 2019-05-14 | 2023-03-28 | Abclonal Science, Inc. | Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye |
| KR20240141247A (ko) * | 2022-01-26 | 2024-09-26 | 주식회사 씨젠 | 샘플 내 타겟 핵산을 검출하는 방법 |
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| US5512482A (en) * | 1990-04-26 | 1996-04-30 | Calgene, Inc. | Plant thioesterases |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5972603A (en) * | 1996-02-09 | 1999-10-26 | President And Fellows Of Harvard College | DNA polymerase with modified processivity |
| US6228628B1 (en) * | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| DE19840771A1 (de) | 1998-08-06 | 2000-02-10 | Lion Bioscience Ag | Thermostabiler in vitro-Komplex mit Polymeraseaktivität |
| DE19937230A1 (de) | 1999-08-06 | 2001-02-08 | Lion Bioscience Ag | Chimäre Proteine |
| US6627424B1 (en) * | 2000-05-26 | 2003-09-30 | Mj Bioworks, Inc. | Nucleic acid modifying enzymes |
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- 2002-11-27 CA CA002468838A patent/CA2468838A1/en not_active Abandoned
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- 2002-11-27 JP JP2003547584A patent/JP4689163B2/ja not_active Expired - Lifetime
- 2002-11-27 DK DK02786844.7T patent/DK1456394T4/da active
- 2002-11-27 EP EP02786844A patent/EP1456394B2/en not_active Expired - Lifetime
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2015
- 2015-08-20 US US14/831,042 patent/US9708598B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
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| JP4689163B2 (ja) | 2011-05-25 |
| US20160060616A1 (en) | 2016-03-03 |
| ES2311638T3 (es) | 2009-02-16 |
| EP1456394A2 (en) | 2004-09-15 |
| DK1456394T3 (da) | 2008-11-24 |
| AU2002351198A1 (en) | 2003-06-10 |
| CA2468838A1 (en) | 2003-06-05 |
| US20040002076A1 (en) | 2004-01-01 |
| EP1456394B1 (en) | 2008-08-27 |
| WO2003046149A3 (en) | 2004-02-19 |
| US8232078B2 (en) | 2012-07-31 |
| US20120295270A1 (en) | 2012-11-22 |
| WO2003046149A2 (en) | 2003-06-05 |
| ATE406456T1 (de) | 2008-09-15 |
| US20140220577A1 (en) | 2014-08-07 |
| US9139873B2 (en) | 2015-09-22 |
| DE60228618D1 (de) | 2008-10-09 |
| US8476045B2 (en) | 2013-07-02 |
| DK1456394T4 (da) | 2011-08-15 |
| EP1456394B2 (en) | 2011-04-27 |
| US9708598B2 (en) | 2017-07-18 |
| JP2005510235A (ja) | 2005-04-21 |
| EP1456394A4 (en) | 2005-12-28 |
| AU2002351198B2 (en) | 2008-08-14 |
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