HK1198704A1 - 具有改進活性的 聚合酶 - Google Patents
具有改進活性的 聚合酶Info
- Publication number
- HK1198704A1 HK1198704A1 HK14112215.8A HK14112215A HK1198704A1 HK 1198704 A1 HK1198704 A1 HK 1198704A1 HK 14112215 A HK14112215 A HK 14112215A HK 1198704 A1 HK1198704 A1 HK 1198704A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- dna
- improved activity
- polymerases
- dna polymerases
- improved
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161568294P | 2011-12-08 | 2011-12-08 | |
| PCT/EP2012/004993 WO2013083264A1 (en) | 2011-12-08 | 2012-12-04 | Dna polymerases with improved activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HK1198704A1 true HK1198704A1 (zh) | 2015-05-29 |
Family
ID=47471678
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HK14112215.8A HK1198704A1 (zh) | 2011-12-08 | 2014-12-04 | 具有改進活性的 聚合酶 |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US8945882B2 (zh) |
| EP (1) | EP2788481B1 (zh) |
| JP (1) | JP6144697B2 (zh) |
| CN (1) | CN103987843B (zh) |
| CA (1) | CA2858264C (zh) |
| ES (1) | ES2668448T3 (zh) |
| HK (1) | HK1198704A1 (zh) |
| WO (1) | WO2013083264A1 (zh) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103492559B (zh) | 2011-04-11 | 2016-07-06 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
| ES2668448T3 (es) | 2011-12-08 | 2018-05-18 | F. Hoffmann-La Roche Ag | ADN polimerasas con actividad mejorada |
| EP2931884B1 (en) * | 2012-12-13 | 2017-03-08 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| EP3218471A1 (en) | 2014-11-14 | 2017-09-20 | Illumina, Inc. | Polymerases |
| US10280411B2 (en) | 2015-10-27 | 2019-05-07 | Pacific Biosciences of California, In.c | Methods, systems, and reagents for direct RNA sequencing |
| CN109154026B (zh) | 2016-05-13 | 2021-09-07 | 豪夫迈·罗氏有限公司 | 用于将dlbcl分类的方法和组合物 |
| WO2018033615A1 (en) | 2016-08-18 | 2018-02-22 | Roche Diagnostics Gmbh | Multiplex allele specific pcr assays for detection of estrogen receptor esr1 mutations |
| EP3541958A1 (en) | 2016-11-15 | 2019-09-25 | Ventana Medical Systems, Inc. | Compositions and methods for prognosing and treating colorectal cancer |
| US10487317B1 (en) * | 2017-03-23 | 2019-11-26 | New England Biolabs, Inc. | Stabilizing 5′-flap endonuclease activity |
| EP3682026A1 (en) | 2017-09-13 | 2020-07-22 | Roche Diagnostics GmbH | Multiplex detection of short nucleic acids |
| WO2019110796A1 (en) | 2017-12-08 | 2019-06-13 | F. Hoffmann-La Roche Ag | Detection of nucleic acids from platelet enriched plasma samples |
| CN111670254B (zh) | 2018-02-20 | 2024-10-11 | 豪夫迈·罗氏有限公司 | 微卫星不稳定性的改进检测 |
| WO2019180137A1 (en) | 2018-03-21 | 2019-09-26 | F. Hoffmann-La Roche Ag | Dna polymerases for efficient and effective incorporation of methylated-dntps |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| EP3822349A4 (en) * | 2018-07-13 | 2022-04-06 | Takara Bio Inc. | DNA POLYMERASE MUTATION SUITABLE FOR NUCLEIC ACID AMPLIFICATION FROM RNA |
| CN112703248A (zh) | 2018-09-13 | 2021-04-23 | 豪夫迈·罗氏有限公司 | 具有改良链置换能力的突变型dna聚合酶 |
| CN111849939B (zh) * | 2020-07-16 | 2022-03-22 | 浙江大学 | 一种缺口dna偏好性高保真聚合酶及其应用 |
| CN114075558B (zh) * | 2020-08-21 | 2024-04-26 | 深圳华大生命科学研究院 | 一种wh54聚合酶及其在rt-pcr中的应用 |
Family Cites Families (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4666848A (en) | 1984-08-31 | 1987-05-19 | Cetus Corporation | Polypeptide expression using a portable temperature sensitive control cassette with a positive retroregulatory element |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US5407800A (en) * | 1986-08-22 | 1995-04-18 | Hoffmann-La Roche Inc. | Reverse transcription with Thermus thermophilus polymerase |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5693502A (en) | 1990-06-11 | 1997-12-02 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| ES2134198T3 (es) | 1990-09-28 | 1999-10-01 | Hoffmann La Roche | Mutaciones en la 5' a 3' exonucleasa de las adn polimerasas. |
| DK0550696T3 (da) | 1990-09-28 | 2001-03-19 | Hoffmann La Roche | Oprenset termostabilt nukleinsyre-polymeraseenzym fra Thermosipho africanus |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| AU7236296A (en) | 1995-09-08 | 1997-03-27 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
| EP0834569A1 (en) | 1996-10-03 | 1998-04-08 | Roche Diagnostics GmbH | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
| US6020128A (en) | 1997-06-10 | 2000-02-01 | United States Of Ameria | DNA polymerase from Treponema pallidum |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| CA2243985C (en) | 1997-09-11 | 2004-08-10 | F. Hoffmann-La Roche Ag | Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes |
| EP0921196A1 (en) | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| EP1088891B1 (en) | 1999-09-28 | 2005-01-12 | Roche Diagnostics GmbH | Thermostable enzyme promoting the fidelity of thermostable DNA polymerases - for improvement of nucleic acid synthesis and amplification in vitro |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| JP2001269188A (ja) | 2000-03-11 | 2001-10-02 | Roche Diagnostics Gmbh | Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体 |
| US7179590B2 (en) | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| US6214557B1 (en) | 2000-06-06 | 2001-04-10 | Washington University | Cold sensitive mutant DNA polymerases |
| CA2459718A1 (en) | 2001-09-14 | 2003-08-14 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
| US20070020622A1 (en) * | 2001-09-14 | 2007-01-25 | Invitrogen Corporation | DNA Polymerases and mutants thereof |
| CA2409775C (en) | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| US7148049B2 (en) | 2002-04-02 | 2006-12-12 | Roche Molecular Systems, Inc. | Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity |
| AU2003237269A1 (en) | 2002-05-31 | 2003-12-19 | University Of Washington | Error-prone DNA polymerase I mutants and methods for targeted random mutagenesis in continuous culture using error-prone DNA polymerase I mutant |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| CA2544841C (en) * | 2003-11-03 | 2014-03-25 | Medical Research Council | Pola dna polymerases capable of abasic site bypass |
| US7417133B2 (en) | 2004-02-27 | 2008-08-26 | Institut Pasteur | Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same |
| US7462475B2 (en) | 2004-05-20 | 2008-12-09 | Dna Poleymerase Technology, Inc. | Use of whole blood in PCR reactions |
| US20060172324A1 (en) | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
| WO2007117331A2 (en) | 2005-12-22 | 2007-10-18 | Ge Healthcare Bio-Sciences Corp. | Novel dna polymerase from thermoanaerobacter tengcongenesis |
| EP2061894A4 (en) | 2006-09-14 | 2010-05-05 | Dna Polymerase Technology Inc | USE OF MUTANT TAQ POLYMERASE ENZYMES FOR DNA AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US8208381B2 (en) | 2007-07-27 | 2012-06-26 | Eg Innovations Pte. Ltd. | Root-cause approach to problem diagnosis in data networks |
| WO2012097318A2 (en) | 2011-01-14 | 2012-07-19 | Kap Abiosystems | Modified dna polymerases for improved amplification |
| CN103492559B (zh) * | 2011-04-11 | 2016-07-06 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
| JP6140182B2 (ja) | 2011-12-08 | 2017-05-31 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| ES2668448T3 (es) | 2011-12-08 | 2018-05-18 | F. Hoffmann-La Roche Ag | ADN polimerasas con actividad mejorada |
| JP6224613B2 (ja) | 2011-12-08 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
-
2012
- 2012-12-04 ES ES12809109.7T patent/ES2668448T3/es active Active
- 2012-12-04 JP JP2014545133A patent/JP6144697B2/ja active Active
- 2012-12-04 CA CA2858264A patent/CA2858264C/en active Active
- 2012-12-04 WO PCT/EP2012/004993 patent/WO2013083264A1/en unknown
- 2012-12-04 CN CN201280059822.6A patent/CN103987843B/zh active Active
- 2012-12-04 EP EP12809109.7A patent/EP2788481B1/en active Active
- 2012-12-05 US US13/706,103 patent/US8945882B2/en active Active
-
2014
- 2014-12-04 HK HK14112215.8A patent/HK1198704A1/zh unknown
- 2014-12-19 US US14/578,228 patent/US9441269B2/en active Active
-
2016
- 2016-07-29 US US15/223,992 patent/US9890366B2/en active Active
-
2018
- 2018-01-04 US US15/862,232 patent/US10294462B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20150184226A1 (en) | 2015-07-02 |
| CN103987843B (zh) | 2015-12-23 |
| WO2013083264A1 (en) | 2013-06-13 |
| JP6144697B2 (ja) | 2017-06-07 |
| US10294462B2 (en) | 2019-05-21 |
| US20170029792A1 (en) | 2017-02-02 |
| US20140051126A1 (en) | 2014-02-20 |
| CN103987843A (zh) | 2014-08-13 |
| US9441269B2 (en) | 2016-09-13 |
| ES2668448T3 (es) | 2018-05-18 |
| WO2013083264A8 (en) | 2014-02-27 |
| US9890366B2 (en) | 2018-02-13 |
| US8945882B2 (en) | 2015-02-03 |
| EP2788481B1 (en) | 2018-03-07 |
| CA2858264C (en) | 2018-01-02 |
| JP2015504651A (ja) | 2015-02-16 |
| EP2788481A1 (en) | 2014-10-15 |
| US20180135033A1 (en) | 2018-05-17 |
| CA2858264A1 (en) | 2013-06-13 |
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