JP4689163B2 - 改良型ポリメラーゼの使用方法 - Google Patents

改良型ポリメラーゼの使用方法 Download PDF

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JP4689163B2
JP4689163B2 JP2003547584A JP2003547584A JP4689163B2 JP 4689163 B2 JP4689163 B2 JP 4689163B2 JP 2003547584 A JP2003547584 A JP 2003547584A JP 2003547584 A JP2003547584 A JP 2003547584A JP 4689163 B2 JP4689163 B2 JP 4689163B2
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glu
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polymerase
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JP2005510235A5 (enExample
JP2005510235A (ja
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ヤン ワン
ホーン ピーター バンダー
レイ シー
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バイオ−ラッド ラボラトリーズ インコーポレーティッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
JP2003547584A 2001-11-28 2002-11-27 改良型ポリメラーゼの使用方法 Expired - Lifetime JP4689163B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US33396601P 2001-11-28 2001-11-28
US60/333,966 2001-11-28
PCT/US2002/038441 WO2003046149A2 (en) 2001-11-28 2002-11-27 Methods of using improved polymerases

Publications (3)

Publication Number Publication Date
JP2005510235A JP2005510235A (ja) 2005-04-21
JP2005510235A5 JP2005510235A5 (enExample) 2006-01-05
JP4689163B2 true JP4689163B2 (ja) 2011-05-25

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JP2003547584A Expired - Lifetime JP4689163B2 (ja) 2001-11-28 2002-11-27 改良型ポリメラーゼの使用方法

Country Status (10)

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US (4) US8232078B2 (enExample)
EP (1) EP1456394B2 (enExample)
JP (1) JP4689163B2 (enExample)
AT (1) ATE406456T1 (enExample)
AU (1) AU2002351198B2 (enExample)
CA (1) CA2468838A1 (enExample)
DE (1) DE60228618D1 (enExample)
DK (1) DK1456394T4 (enExample)
ES (1) ES2311638T5 (enExample)
WO (1) WO2003046149A2 (enExample)

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* Cited by examiner, † Cited by third party
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US7820808B2 (en) 2003-04-01 2010-10-26 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
EP1706502A4 (en) * 2003-11-26 2007-05-23 Eppendorf Ag METHODS AND COMPOSITIONS FOR IN VITRO AMPLIFICATION OF EXTRACHROMOSOMIC NUCLEIC ACID
WO2006074288A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity
WO2006074233A2 (en) * 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
JP4899618B2 (ja) * 2006-05-01 2012-03-21 ソニー株式会社 二本鎖核酸検出方法、ハイブリダイゼーション検出方法
KR100825279B1 (ko) * 2006-11-30 2008-04-25 한국해양연구원 Ｄｎα 중합효소 활성 증가 단백질 및 이를 암호화 하는유전자
EP3029146B1 (en) * 2007-11-27 2018-08-01 Bio-rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
EP2240576B1 (en) * 2008-01-11 2012-10-03 Genesys Ltd Cren7 chimeric protein
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
EP3101124B1 (en) 2008-11-03 2019-06-19 Kapa Biosystems, Inc. Modified type a dna polymerases
JP5818688B2 (ja) 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
US20130022980A1 (en) * 2009-02-04 2013-01-24 Lucigen Corporation Rna- and dna-copying enzymes
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP2694670B1 (en) 2011-04-08 2017-07-19 Bio-Rad Laboratories, Inc. Pcr reaction mixtures with decreased non-specific activity
US8697410B2 (en) 2011-04-08 2014-04-15 Bio-Rad Laboratories, Inc. SSO7-polymerase conjugates with decreased non-specific activity
US8470573B2 (en) * 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
WO2016028671A1 (en) 2014-08-19 2016-02-25 Bio-Rad Laboratories, Inc. Rna amplification methods
US9963687B2 (en) * 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
EP3450558B1 (en) * 2014-08-27 2020-09-30 New England Biolabs, Inc. Synthon formation
EP3259373B1 (en) 2015-02-17 2022-07-27 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
CN111556900A (zh) 2017-12-22 2020-08-18 赛默飞世尔科技波罗的海封闭股份公司 包括胺的聚合酶链反应组合物
CN116200364A (zh) * 2019-03-10 2023-06-02 武汉爱博泰克生物科技有限公司 用于在提高的盐浓度或体液中扩增的突变型Taq聚合酶
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
KR20240141247A (ko) * 2022-01-26 2024-09-26 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19840771A1 (de) 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
DE19937230A1 (de) 1999-08-06 2001-02-08 Lion Bioscience Ag Chimäre Proteine
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes

Also Published As

Publication number Publication date
US20160060616A1 (en) 2016-03-03
ES2311638T3 (es) 2009-02-16
EP1456394A2 (en) 2004-09-15
DK1456394T3 (da) 2008-11-24
AU2002351198A1 (en) 2003-06-10
CA2468838A1 (en) 2003-06-05
US20040002076A1 (en) 2004-01-01
EP1456394B1 (en) 2008-08-27
WO2003046149A3 (en) 2004-02-19
US8232078B2 (en) 2012-07-31
US20120295270A1 (en) 2012-11-22
WO2003046149A2 (en) 2003-06-05
ATE406456T1 (de) 2008-09-15
US20140220577A1 (en) 2014-08-07
US9139873B2 (en) 2015-09-22
ES2311638T5 (es) 2011-09-08
DE60228618D1 (de) 2008-10-09
US8476045B2 (en) 2013-07-02
DK1456394T4 (da) 2011-08-15
EP1456394B2 (en) 2011-04-27
US9708598B2 (en) 2017-07-18
JP2005510235A (ja) 2005-04-21
EP1456394A4 (en) 2005-12-28
AU2002351198B2 (en) 2008-08-14

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