ES2191575T3 - Un metodo para escrutar sustancias en cuanto a su efecto sobre una translocacion intracelular. - Google Patents
Un metodo para escrutar sustancias en cuanto a su efecto sobre una translocacion intracelular.Info
- Publication number
- ES2191575T3 ES2191575T3 ES01204477T ES01204477T ES2191575T3 ES 2191575 T3 ES2191575 T3 ES 2191575T3 ES 01204477 T ES01204477 T ES 01204477T ES 01204477 T ES01204477 T ES 01204477T ES 2191575 T3 ES2191575 T3 ES 2191575T3
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| US6121047A (en) * | 1999-07-21 | 2000-09-19 | Isis Pharmaceuticals Inc. | Antisense modulation of SHP-1 expression |
| US6489151B1 (en) * | 2000-03-27 | 2002-12-03 | The Research Foundation Of State University Of New York | Biologically active alternative form of the IKKα IκB kinase |
| EP1300418A1 (en) * | 2001-10-04 | 2003-04-09 | Erasmus Universiteit Rotterdam | Gene regulation by oligopeptides |
| KR100414641B1 (ko) | 2000-04-07 | 2004-01-13 | 동부한농화학 주식회사 | 형질전환식물체의 생체분석방법 및 그를 이용한 시스템 |
| AU2001250286A1 (en) * | 2000-04-17 | 2001-10-30 | Bioimage A/S | Live cell procedures to identify compounds modulating intracellular distributionof phosphodiesterase (pde) enzymes |
| AU2001252109A1 (en) * | 2000-04-26 | 2001-11-07 | Bioimage A/S | Chemical signal enhancement of dynamic intensity-based intracellular protein- and fluorophore-based redistribution assays for drug screening |
| US20020049544A1 (en) * | 2000-06-23 | 2002-04-25 | Cytokinetics, Inc. | Image analysis for phenotyping sets of mutant cells |
| US7282347B2 (en) | 2000-07-04 | 2007-10-16 | Fisher Bioimage Aps | Method for extracting quantitative information relating to interactions between cellular components |
| US7625951B2 (en) | 2000-07-13 | 2009-12-01 | University Of Kentucky Research Foundation | Stimuli-responsive hydrogel microdomes integrated with genetically engineered proteins for high-throughput screening of pharmaceuticals |
| DE10037759A1 (de) * | 2000-08-03 | 2002-07-04 | Gruenenthal Gmbh | Screeningverfahren |
| KR100414781B1 (ko) * | 2000-11-24 | 2004-01-13 | 재단법인서울대학교산학협력재단 | ＮＦ-κＢ 발현을 인간 피부세포에서 정량적으로 측정하기위한 안정한 분석시스템 및 그의 용도 |
| US7218764B2 (en) | 2000-12-04 | 2007-05-15 | Cytokinetics, Inc. | Ploidy classification method |
| US7016787B2 (en) | 2001-02-20 | 2006-03-21 | Cytokinetics, Inc. | Characterizing biological stimuli by response curves |
| US6956961B2 (en) | 2001-02-20 | 2005-10-18 | Cytokinetics, Inc. | Extracting shape information contained in cell images |
| US7033790B2 (en) | 2001-04-03 | 2006-04-25 | Curagen Corporation | Proteins and nucleic acids encoding same |
| CA2453528C (en) | 2001-08-01 | 2011-07-26 | Cellomics, Inc. | Novel fusion proteins and assays for molecular binding |
| WO2003050535A2 (de) * | 2001-12-10 | 2003-06-19 | Evotec Oai Ag | Verfahren zur bestimmung von einflüssen unphysiologischer verbindungen, deren derivate und abbauprodukte auf organismen, organe und zellen |
| US7176303B2 (en) * | 2003-11-06 | 2007-02-13 | Isis Pharmaceuticals, Inc. | Modulation of STAT5 expression |
| US7786084B2 (en) * | 2001-12-21 | 2010-08-31 | Biotempt B.V. | Treatment of burns |
| US7560433B2 (en) | 2001-12-21 | 2009-07-14 | Biotempt B.V. | Treatment of multiple sclerosis (MS) |
| US20080318871A1 (en) * | 2001-12-21 | 2008-12-25 | Khan Nisar A | Treatment of neurological disorders |
| US20080242837A1 (en) * | 2001-12-21 | 2008-10-02 | Khan Nisar A | Peptide compositions |
| US20030220257A1 (en) * | 2001-12-21 | 2003-11-27 | Robbert Benner | Treatment of trauma |
| CA2413475C (en) | 2002-04-25 | 2010-07-27 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Zap-70 expression as a marker for chronic lymphocytic leukemia/small lymphocytic lymphoma (cll/sll) |
| GB0216674D0 (en) * | 2002-07-18 | 2002-08-28 | Amersham Biosciences Uk Ltd | Functional screening method |
| US8168568B1 (en) | 2003-03-10 | 2012-05-01 | The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Combinatorial therapy for protein signaling diseases |
| US7235353B2 (en) | 2003-07-18 | 2007-06-26 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
| US20050014217A1 (en) | 2003-07-18 | 2005-01-20 | Cytokinetics, Inc. | Predicting hepatotoxicity using cell based assays |
| US7246012B2 (en) | 2003-07-18 | 2007-07-17 | Cytokinetics, Inc. | Characterizing biological stimuli by response curves |
| US20050060100A1 (en) * | 2003-09-15 | 2005-03-17 | Becton Dickinson And Company | Computer software and algorithms for systems biologically linked to cellular phenotype |
| US20050059083A1 (en) * | 2003-09-15 | 2005-03-17 | Becton Dickinson And Company | High throughput method to identify ligands for cell attachment |
| US20090227505A1 (en) * | 2004-01-07 | 2009-09-10 | Biotempt B.V. | Methods and uses for protein breakdown products |
| EP2400030B1 (en) | 2004-05-18 | 2016-01-20 | The Board of Trustees of the Leland Stanford Junior University | Detection of protein translocation by beta-galactosidase reporter fragment complementation |
| US7323318B2 (en) | 2004-07-15 | 2008-01-29 | Cytokinetics, Inc. | Assay for distinguishing live and dead cells |
| US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
| SG163567A1 (en) * | 2005-07-05 | 2010-08-30 | Biotempt Bv | Treatment of tumors |
| JP2009513150A (ja) * | 2005-10-28 | 2009-04-02 | ジーイー・ヘルスケア・ユーケイ・リミテッド | シグナリングアッセイ及び株化細胞 |
| JP2009526519A (ja) * | 2006-01-17 | 2009-07-23 | セルーメン、インコーポレイテッド | 生物学的システム応答の予測方法 |
| EP1864692A1 (en) * | 2006-06-07 | 2007-12-12 | Biotempt B.V. | Use of peptides for the control of radiation injury |
| CN101484806A (zh) | 2006-05-17 | 2009-07-15 | 协乐民公司 | 一种对组织进行自动分析的方法 |
| US20100112602A1 (en) * | 2006-11-10 | 2010-05-06 | Taylor Lansing D | Protein-Protein Interaction Biosensors and Methods of Use Thereof |
| US20100178652A1 (en) * | 2007-01-05 | 2010-07-15 | Litherland Sally A | Materials and Methods for the Detection, Prevention and Treatment of Autoimmune Disease |
| WO2008100140A1 (en) * | 2007-02-12 | 2008-08-21 | Biotempt B.V. | Treatment of trauma hemorrhage with short oligopeptides |
| US20080217002A1 (en) * | 2007-03-07 | 2008-09-11 | Floyd Randolph Simonds | Sand control screen having a micro-perforated filtration layer |
| JP5438962B2 (ja) * | 2008-12-25 | 2014-03-12 | シスメックス株式会社 | 細胞画像表示装置 |
| US8574837B2 (en) | 2008-12-29 | 2013-11-05 | President And Fellows Of Harvard College | System for screening agonists/antagonists of cellular signaling pathways |
| KR101088605B1 (ko) | 2009-06-30 | 2011-11-30 | 한국산업기술대학교산학협력단 | 2대의 카메라를 이용한 세포 조작 방법 |
| JP5089713B2 (ja) * | 2010-01-18 | 2012-12-05 | シャープ株式会社 | 画像圧縮装置、圧縮画像出力装置、画像圧縮方法、コンピュータプログラム及び記録媒体 |
| CA2830501C (en) | 2011-03-17 | 2023-10-17 | Cernostics, Inc. | Systems and compositions for diagnosing barrett's esophagus and methods of using the same |
| EP2825887A4 (en) | 2012-03-15 | 2015-08-19 | Montana Molecular Llc | GENETICALLY-CODED, FLUORESCENT SENSORS FOR THE DETECTION OF INTRA-CELLULAR SIGNALS BY DIACYLGLYCEROL SIGNAL PATHS |
| EP2682187B1 (en) * | 2012-07-02 | 2017-09-13 | Molecular Devices, LLC | Selection method and apparatus |
| CA2929392A1 (en) | 2013-11-04 | 2015-05-07 | Montana Molecular Llc | Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through camp pathways |
| DE102014012130B3 (de) | 2014-08-13 | 2015-03-05 | Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung | Nachweisverfahren unter Verwendung von rekombinanten lebenden Zellen zum Nachweis von xenobiotischen Stoffen sowie Anordnung und Testkit zur Durchführung des Nachweisverfahrens |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT321257B (de) | 1971-05-26 | 1975-03-25 | Koreska Gmbh W | Anlage zur Rückgewinnung flüchtiger Lösungsmittel |
| JPS57198544A (en) * | 1981-05-28 | 1982-12-06 | Sony Corp | Manufacture of magnetic recording medium |
| DE3240063A1 (de) | 1981-11-02 | 1983-05-26 | Basf Ag, 6700 Ludwigshafen | Vorrichtung zur herstellung eines magnetogrammtraegers |
| JPS5880136A (ja) * | 1981-11-02 | 1983-05-14 | Fuji Photo Film Co Ltd | 磁気記録材料の製造方法 |
| JPH0656656B2 (ja) * | 1983-10-03 | 1994-07-27 | 富士写真フイルム株式会社 | 磁気記録媒体の製造方法 |
| JPS6292132A (ja) * | 1985-10-18 | 1987-04-27 | Fuji Photo Film Co Ltd | 磁気記録媒体の製造方法 |
| US4923766A (en) * | 1988-03-29 | 1990-05-08 | Canon Kabushiki Kaisha | Process for preparing magnetic recording |
| GB8916806D0 (en) * | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
| DE4009797A1 (de) * | 1990-03-27 | 1991-10-02 | Pagendarm Gmbh | Verfahren und anordnung zum kondensieren von dampffoermigen substanzen |
| CA2157774A1 (en) * | 1993-04-07 | 1994-10-13 | Christopher John Marshall | Methods for screening of substances for therapeutic activity and yeast for use therein |
| JP3810791B2 (ja) * | 1993-09-10 | 2006-08-16 | ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク | 緑色蛍光タンパク質の使用 |
| US6184205B1 (en) * | 1994-07-22 | 2001-02-06 | University Of North Carolina At Chapel Hill | GRB2 SH3 binding peptides and methods of isolating and using same |
| JP4215274B2 (ja) * | 1994-07-22 | 2009-01-28 | ザ ユニバーシティー オブ ノースカロライナ アット チャペル ヒル | Ｓｒｃ ＳＨ３結合性ペプチドとその分離法と利用法 |
| US5874231A (en) * | 1994-08-22 | 1999-02-23 | Mcgill University | Methods of screening for non-hormone compounds which effect modulation of polypeptide translation |
| DK0815257T3 (da) * | 1995-01-31 | 2002-04-22 | Bioimage As | Fremgangsmåde til karakterisering af biologisk aktive stoffer |
| US5958713A (en) | 1995-01-31 | 1999-09-28 | Novo Nordisk A/S | Method of detecting biologically active substances by using green fluorescent protein |
| JP3535177B2 (ja) * | 1995-09-22 | 2004-06-07 | バイオイメージ・アクティーゼルスカブ | 緑色蛍光性タンパクであるｇｆｐの新規な変種 |
| WO1997020931A1 (en) * | 1995-12-08 | 1997-06-12 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Method and compositions for monitoring dna binding molecules in living cells |
| US5874304A (en) * | 1996-01-18 | 1999-02-23 | University Of Florida Research Foundation, Inc. | Humanized green fluorescent protein genes and methods |
| US5989835A (en) * | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
| ATE469981T1 (de) * | 1996-07-16 | 2010-06-15 | Univ California | Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten |
| AU5090498A (en) * | 1997-01-07 | 1998-08-03 | California Institute Of Technology | Optical sensors of cell signaling |
| US6416959B1 (en) * | 1997-02-27 | 2002-07-09 | Kenneth Giuliano | System for cell-based screening |
| AU730100B2 (en) * | 1997-02-27 | 2001-02-22 | Cellomics, Inc. | A system for cell-based screening |
| DK1435519T3 (da) * | 1997-04-07 | 2007-06-11 | Fisher Bioimage Aps | Fremgangsmåde til screening af substanser for effekt på cAMP-niveauer baseret på intracellulær translokation af PKA |
| WO2000049183A1 (en) * | 1999-02-17 | 2000-08-24 | Clontech Laboratories, Inc. | FLUORESCENCE RESONANCE ENERGY TRANSFER DETECTION OF cAMP IN LIVING CELLS USING GFP VARIANTS |
| US6573059B1 (en) * | 1999-06-04 | 2003-06-03 | Rmf Dictagene S.A. | Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements |
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- 1998-04-07 DE DE1199564T patent/DE1199564T1/de active Pending
- 1998-04-07 AT AT98913541T patent/ATE215227T1/de not_active IP Right Cessation
- 1998-04-07 JP JP54227698A patent/JP2001522454A/ja active Pending
- 1998-04-07 ES ES01204477T patent/ES2191575T3/es not_active Expired - Lifetime
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- 1998-04-07 WO PCT/DK1998/000145 patent/WO1998045704A2/en not_active Ceased
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- 1998-04-07 AT AT01204477T patent/ATE269975T1/de not_active IP Right Cessation
- 1998-04-07 PT PT98913541T patent/PT986753E/pt unknown
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- 1998-04-07 DE DE69837118T patent/DE69837118T2/de not_active Expired - Fee Related
- 1998-04-07 PT PT01204477T patent/PT1199564E/pt unknown
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- 1998-04-07 ES ES98913541T patent/ES2173573T5/es not_active Expired - Lifetime
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