ES2191028T3 - Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado. - Google Patents

Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado.

Info

Publication number
ES2191028T3
ES2191028T3 ES94904344T ES94904344T ES2191028T3 ES 2191028 T3 ES2191028 T3 ES 2191028T3 ES 94904344 T ES94904344 T ES 94904344T ES 94904344 T ES94904344 T ES 94904344T ES 2191028 T3 ES2191028 T3 ES 2191028T3
Authority
ES
Spain
Prior art keywords
plants
fructosyltransferase
modified
plant
plant cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94904344T
Other languages
English (en)
Inventor
Josephus Christianus Smeekens
Michael Johannes Marcu Ebskamp
Petrus Jacobus Weisbeek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STICHTING SCHEIKUNDIG ONDERZOE
Original Assignee
STICHTING SCHEIKUNDIG ONDERZOE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL9300646A external-priority patent/NL9300646A/nl
Application filed by STICHTING SCHEIKUNDIG ONDERZOE filed Critical STICHTING SCHEIKUNDIG ONDERZOE
Application granted granted Critical
Publication of ES2191028T3 publication Critical patent/ES2191028T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/244—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from corms, tubers or roots, e.g. glucomannan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Paper (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Packaging Of Special Articles (AREA)

Abstract

EL INVENTO TRATA DE UN METODO PARA OBTENER PLANTAS TRANSGENICAS QUE MUESTRAN UN MODELO DE FRUCTAN MODIFICADO COMPARADAS CON PLANTAS NO TRANFORMADAS, COMPRENDIENDO LOS SIGUIENTES PASOS: A) PREPARAR UNA CONSTRUCCION DE DNA QUE COMPRENDA UN O MAS GENES FRUCTOSILTRANSFERASA, O VERSIONES MODIFICADAS DE LOS MISMOS, SIENDO LIGABLES OPERACIONALMENTE A UNA SECUENCIA PROMOTORA ACTIVA EN PLANTAS Y UNA SECUENCIA TERMINADORA ACTIVA EN PLANTAS; B) TRANSFORMAR UNA CELULA DE PLANTA CON LA CONSTRUCCION; Y C) REGENERAR UNA PLANTA TRANSGENICA DE LA CELULA TRANSFORMADA DE LA PLANTA. PREFERIBLEMENTE, LA REGION 5`INAMOVIBLE DEL GEN FRUCTOSILTRANSFERASA SE MODIFICA DE TAL MANERA QUE LA EXPRESION DE LA FRUCTOSILTRANSFERASA NO SE VEA AFECTADA NEGATIVAMENTE. LA CONSTRUCCION DE DNA, PREFERIBLEMENTE, TAMBIEN COMPRENDE LA SECUENCIA OBJETIVA DE MOVIMIENTO DEL GEN DE LA FRUCTOSILTRANSFERASA PARA DIRIGIRLA AL TEJIDO ESPECIFICO DE LA PLANTA O AL COMPARTIMENTO CELULAR DE LA MISMA. LA FRUCTOSILTRANSFERASA PRODUCIDA EN LAS PLANTAS TRANSGENICAS CAMBIANDO EL MODELO FRUCTAN DE LAS PLANTAS PRINCIPALES AL COMPORTAMIENTO DE DIFERENTES PLANTAS DEBIDO A ALTERACIONES EN SU ORIGEN Y EN LA PRODUCCION, O INCREMENTA LA TOLERANCIA A LA SEQUIA, FRIO, U OTROS FACTORES DE ESTRES, CONTIENE UN INDICE MAYOR DE SEQUEDAD, MEJOR SABOR Y ALMACENAMIENTO Y SE MEJORA SU VALOR NUTRITIVO. LAS PLANTAS PUEDEN SER TAMBIEN USADAS COMO MATERIA CRUDA PARA LA PRODUCCION DE FRUCTAN.
ES94904344T 1992-12-28 1993-12-28 Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado. Expired - Lifetime ES2191028T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92204098 1992-12-28
NL9300646A NL9300646A (nl) 1992-12-28 1993-04-15 Werkwijze voor het verkrijgen van transgene planten welke een gemodificeerd fructaan-patroon vertonen.

Publications (1)

Publication Number Publication Date
ES2191028T3 true ES2191028T3 (es) 2003-09-01

Family

ID=26131899

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94904344T Expired - Lifetime ES2191028T3 (es) 1992-12-28 1993-12-28 Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado.

Country Status (12)

Country Link
US (2) US6025542A (es)
EP (1) EP0677112B1 (es)
JP (1) JPH08507918A (es)
AT (1) ATE235557T1 (es)
AU (1) AU5843794A (es)
DE (1) DE69332803T2 (es)
DK (1) DK0677112T3 (es)
ES (1) ES2191028T3 (es)
HU (1) HU225704B1 (es)
PL (1) PL178789B1 (es)
PT (1) PT677112E (es)
WO (1) WO1994014970A1 (es)

Families Citing this family (37)

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DE4227061A1 (de) * 1992-08-12 1994-02-17 Inst Genbiologische Forschung DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
AU5843794A (en) * 1992-12-28 1994-07-19 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
KR960705938A (ko) * 1993-11-09 1996-11-08 미리암 디. 메코너헤이 유전자 전환된 프럭탄 축적 작물 및 그의 제조 방법(Transgenic Fructan Accumulating Crops and Method for Their Production)
NL9401140A (nl) * 1994-07-08 1996-02-01 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
NL1000064C1 (nl) 1994-07-08 1996-01-08 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
JP3840259B2 (ja) * 1995-01-06 2006-11-01 プラント リサーチ インターナショナル ベスローテン フェンノートシャップ 炭水化物ポリマー合成酵素をコードするｄｎａ配列及びトランスジェニック植物を製造するための方法
KR19990076833A (ko) 1995-12-28 1999-10-25 도리이 신이찌로 내온도성 식물의 제조방법
NL1002275C2 (nl) * 1996-02-07 1997-08-08 Have D J Van Der Bv Modificatie van polysacchariden.
US6833491B2 (en) 1996-02-07 2004-12-21 D. J. Van Der Have B.V. Modification of polysaccharides
DE19617687C2 (de) * 1996-05-03 2000-11-16 Suedzucker Ag Verfahren zur Herstellung transgener, Inulin erzeugender Pflanzen
BE1010449A3 (fr) 1996-08-01 1998-08-04 Raffinerie Tirlemontoise Sa Procede de preparation d'une composition polydipersee de saccharides, composition polydispersee de saccharides, produit alimentaire, pharmaceutique et/ou cosmetique comprenant ladite composition polydispersee.
DE19708774A1 (de) * 1997-03-04 1998-09-17 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme die Fructosylpolymeraseaktivität besitzen
DE19749122A1 (de) 1997-11-06 1999-06-10 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen
US6365800B1 (en) 1998-03-12 2002-04-02 E. I. Du Pont Nemours & Company Transgenic crops accumulating fructose polymers and methods for their production
CN1116417C (zh) * 1998-04-08 2003-07-30 中国科学院遗传研究所 用转基因技术提高植物耐盐性的方法
DE19840028A1 (de) 1998-09-02 2000-03-09 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen, und deren Verwendung
JP2000350583A (ja) * 1999-03-25 2000-12-19 Hokkaido National Agricultural Experiment Station 低温発現型フルクタン代謝酵素遺伝子
US20020144310A1 (en) * 2000-01-28 2002-10-03 Lightfoot David A. Isolated polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and soybean sudden death syndrome and methods employing same
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
AUPQ815500A0 (en) 2000-06-14 2000-07-06 Molecular Plant Breeding Nominees Ltd Modification of fructan biosynthesis
US6791015B2 (en) 2000-10-30 2004-09-14 E. I. Du Pont De Nemours And Company Fructan biosynthetic enzymes
MXPA03005645A (es) * 2000-12-21 2004-04-21 Suedzucker Ag Procedimiento para la preparacion de carbohidratos.
WO2003000854A2 (en) * 2001-06-25 2003-01-03 Ses Europe N.V./S.A. Double fructan beets
US20050150008A1 (en) * 2001-11-07 2005-07-07 Agrigenesis Biosciences Limited Compositions isolated from forage grasses and methods of use
NZ568813A (en) * 2001-11-07 2010-04-30 Genesis Res & Dev Corp Ltd Sucrose phosphate synthases isolated from the grasses lolium perenne and festuca arundinacea
US20040133944A1 (en) * 2003-01-08 2004-07-08 Delta And Pine Land Company Seed oil suppression to enhance yield of commercially important macromolecules
AU2003902253A0 (en) * 2003-05-12 2003-05-29 The University Of Queensland Method for increasing product yield
US7682829B2 (en) * 2003-05-30 2010-03-23 Monsanto Technology Llc Methods for corn transformation
EA014887B1 (ru) * 2003-06-17 2011-02-28 Сембайосиз Джинетикс Инк. Способ экспрессии инсулина в семенах растения, способ получения семян растений, содержащих инсулин, и растения, способные производить семена, содержащие инсулин
US7317098B2 (en) * 2004-07-01 2008-01-08 The Board Of Trustees Of Michigan State University Ryegrass CBF3 gene: identification and isolation
JP4714894B2 (ja) * 2005-06-22 2011-06-29 独立行政法人農業・食品産業技術総合研究機構 耐冷性植物及びその開発方法
EP2337848A4 (en) 2008-09-15 2012-05-09 Agriculture Victoria Serv Pty MODIFICATION OF FRUCTAN BIOYSYNTHESIS, INCREASED PLANT BIOMASS AND INCREASED PRODUCTIVITY OF BIOCHEMICAL WAYS IN A PLANT
US9840695B2 (en) 2009-04-28 2017-12-12 Agriculture Victoria Services Pty Ltd Plant technology
US10316327B2 (en) 2013-03-15 2019-06-11 Yi Li Genetically modified plants that are insect-resistant and/or rot resistant
WO2017059902A1 (en) 2015-10-07 2017-04-13 Biontech Rna Pharmaceuticals Gmbh 3' utr sequences for stabilization of rna

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JPS5953834B2 (ja) * 1982-05-19 1984-12-27 明治製菓株式会社 ビフイズス菌増殖促進物質
JPS6034134A (ja) * 1983-08-05 1985-02-21 Meiji Seika Kaisha Ltd フラクトオリゴ糖含有飼料ならびにこれを用いて家畜を飼育する方法
US4801540A (en) * 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
IL90713A0 (en) * 1988-06-21 1990-01-18 Calgene Inc Methods and compositions for altering physical characteristics of fruit and fruit products
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BE1003826A3 (fr) * 1990-02-23 1992-06-23 Raffinerie Tirlemontoise Sa Fructo-oligosaccharides ramifies, procede pour leur obtention et utilisation des produits les contenant.
JPH0670075B2 (ja) * 1990-08-28 1994-09-07 ホクレン農業協同組合連合会 １―ケストース結晶およびその製造方法
US5206355A (en) * 1991-09-30 1993-04-27 The University Of Montana Preparation of trisaccharides (kestoses) and polymers by pyrolysis of amorphous sucrose
DE4227061A1 (de) * 1992-08-12 1994-02-17 Inst Genbiologische Forschung DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
AU5843794A (en) * 1992-12-28 1994-07-19 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
US5641759A (en) * 1993-05-26 1997-06-24 Purdue Research Foundation Animal husbandry methods and compositions therefor
DE4330960C2 (de) * 1993-09-09 2002-06-20 Aventis Cropscience Gmbh Kombination von DNA-Sequenzen, die in Pflanzenzellen und Pflanzen die Bildung hochgradig amylosehaltiger Stärke ermöglichen, Verfahren zur Herstellung dieser Pflanzen und die daraus erhaltbare modifizierte Stärke
KR960705938A (ko) * 1993-11-09 1996-11-08 미리암 디. 메코너헤이 유전자 전환된 프럭탄 축적 작물 및 그의 제조 방법(Transgenic Fructan Accumulating Crops and Method for Their Production)

Also Published As

Publication number Publication date
DE69332803T2 (de) 2004-03-04
US6025542A (en) 2000-02-15
PL309606A1 (en) 1995-10-30
PL178789B1 (pl) 2000-06-30
HU9501912D0 (en) 1995-08-28
HUT71782A (en) 1996-02-28
EP0677112B1 (en) 2003-03-26
DE69332803D1 (de) 2003-04-30
ATE235557T1 (de) 2003-04-15
PT677112E (pt) 2003-08-29
AU5843794A (en) 1994-07-19
HU225704B1 (en) 2007-06-28
EP0677112A1 (en) 1995-10-18
DK0677112T3 (da) 2003-07-21
WO1994014970A1 (en) 1994-07-07
US5986173A (en) 1999-11-16
JPH08507918A (ja) 1996-08-27

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