ES2185620T3 - Un transformante capaz de producir d-aminoacido oxidasa. - Google Patents

Un transformante capaz de producir d-aminoacido oxidasa.

Info

Publication number
ES2185620T3
ES2185620T3 ES93202219T ES93202219T ES2185620T3 ES 2185620 T3 ES2185620 T3 ES 2185620T3 ES 93202219 T ES93202219 T ES 93202219T ES 93202219 T ES93202219 T ES 93202219T ES 2185620 T3 ES2185620 T3 ES 2185620T3
Authority
ES
Spain
Prior art keywords
trigonopsis variabilis
transformant
cephalosporin
amino acid
trigonopsis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93202219T
Other languages
English (en)
Inventor
Kaoru Furuya
Akio Matsuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Kasei Corp
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Kasei Corp
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Kasei Corp, Asahi Chemical Industry Co Ltd filed Critical Asahi Kasei Corp
Application granted granted Critical
Publication of ES2185620T3 publication Critical patent/ES2185620T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/03—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12Y104/03003—D-Amino-acid oxidase (1.4.3.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE PRESENTA UN TRIGONOPSIS VARIABILIS TRANSFORMADO CON UN DNA RECOMBINANTE QUE CONTIENE UN GEN DE OXIDAD D-AMINOACIDO CAPAZ DE EXPRESAR TRIGONOPSIS VARIABILIS. TAMBIEN SE PRESENTA UN PROCESO PARA TRANSFORMAR TRIGONOPSIS VARIABILIS Y UN PROCESO PARA PREPARAR ACIDO 7-BETA-(5-CARBOXI EFATLOSPORANICO UTILIZANDO UN TRANSFORMANTE DE TRIGNOPSIS VARIABILIS. EL TRANSFORMANTE DE TRIGONOPSIS VARIABILIS MUESTRA ALTA ACTIVIDAD DAO Y BAJA ACTIVIDAD DE UNA ESTERASA LA CUAL INTERFIERE EN LA PREPARACION DE CEFALOSPORINA C. POR CONSIGUIENTE, EL TRIGONOPSIS VARIABILIS DE L A PRESENTE INVENCION PERMITE LA PRODUCCION DE CEFALOSPORINA C. ADEMAS, EL TRIGONOPSIS VARIABILIS DE LA INVENCION PUEDE SER USADO PARA LA PREPARACION DE CAFALOSPRINA C TRATADO SIMPLEMENTE LAS CELULAS CON TOLUENO DE MANERA QUE SU USO SEA PRACTICO A GRAN ESCALA.
ES93202219T 1992-07-27 1993-07-27 Un transformante capaz de producir d-aminoacido oxidasa. Expired - Lifetime ES2185620T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP19994892 1992-07-27

Publications (1)

Publication Number Publication Date
ES2185620T3 true ES2185620T3 (es) 2003-05-01

Family

ID=16416266

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93202219T Expired - Lifetime ES2185620T3 (es) 1992-07-27 1993-07-27 Un transformante capaz de producir d-aminoacido oxidasa.

Country Status (8)

Country Link
US (1) US5453374A (es)
EP (1) EP0583817B1 (es)
KR (1) KR0131063B1 (es)
CN (1) CN1064079C (es)
AT (1) ATE226980T1 (es)
CA (1) CA2100987C (es)
DE (1) DE69332447T2 (es)
ES (1) ES2185620T3 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2275925B (en) * 1993-03-08 1996-08-14 Asahi Chemical Ind A process for converting cephalosporin C to glutaryl-7-aminocephalosporanic acid
IT1275909B1 (it) * 1995-03-07 1997-10-24 Mirella Pilone Frammento di dna codificante d-amminoacido ossidasi
ES2109194B1 (es) * 1996-04-22 1998-07-16 Antibioticos Sa Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.
CA2292844A1 (en) * 1997-06-25 1998-12-30 Bristol-Myers Squibb Company D-amino acid oxidase promoter from t. variabilis
ES2131018B1 (es) 1997-09-25 2000-04-01 Antibioticos Sau Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.
KR100246820B1 (ko) * 1997-12-30 2000-03-15 정수련 신균주 트리고높시스 배리아빌리스에 의한 에리쓰리톨의 제조방법
AU2002310863A1 (en) * 2001-04-19 2002-11-05 Bioferma Murcia, S.A. Enzymatic process for preparing cephalosporanic acid derivatives using alpha-ketoacid derivatives
CN100342014C (zh) * 2004-04-08 2007-10-10 百瑞全球有限公司 重组d－氨基酸氧化酶
CA2621422A1 (en) * 2005-09-13 2007-03-22 Suntory Limited Esterase gene and use thereof
CN102505035B (zh) * 2011-10-25 2014-07-09 石药集团中诺药业（石家庄）有限公司 一种3-去乙酰基-7-氨基头孢烷酸的制备工艺
CN103710361B (zh) * 2013-07-12 2015-07-15 广西大学 一种编码D-氨基酸氧化酶的基因daoE及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1385685A (en) * 1971-04-21 1975-02-26 Glaxo Lab Ltd Cephalosporin derivatives
JPS5535118A (en) * 1978-08-31 1980-03-12 Matsushita Electric Works Ltd Driving shaft fixing apparatus of diaphragm pump
JPS62262994A (ja) * 1986-05-12 1987-11-16 Asahi Chem Ind Co Ltd Ｄ−アミノ酸オキシダ−ゼ遺伝子
JPH07108225B2 (ja) * 1986-09-16 1995-11-22 旭化成工業株式会社 Ｄ−アミノ酸オキシダ−ゼ遺伝子
ES2075056T3 (es) * 1988-10-13 1995-10-01 Fujisawa Pharmaceutical Co Oxidasa de los d-aminoacidos.
JP2876652B2 (ja) * 1988-10-13 1999-03-31 藤沢薬品工業株式会社 Ｄ―アミノ酸オキシダーゼ
DE69019967T2 (de) * 1989-04-04 1996-02-22 Biopure Corp Enzymatisches verfahren zur herstellung von 7-aminocephalosporansäure.
DK0409521T3 (da) * 1989-07-19 1996-02-05 Lilly Co Eli Forbedret enzymatisk oxidation af Ceph C til glutaryl-7-aminocephalosporansyre
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
DE4028119C1 (es) * 1990-09-05 1991-12-05 Hoechst Ag, 6230 Frankfurt, De

Also Published As

Publication number Publication date
KR940002352A (ko) 1994-02-17
DE69332447T2 (de) 2003-07-03
EP0583817A3 (en) 1995-01-11
US5453374A (en) 1995-09-26
ATE226980T1 (de) 2002-11-15
DE69332447D1 (de) 2002-12-05
CA2100987A1 (en) 1994-01-28
EP0583817A2 (en) 1994-02-23
EP0583817B1 (en) 2002-10-30
CN1087118A (zh) 1994-05-25
CN1064079C (zh) 2001-04-04
CA2100987C (en) 1999-06-15
KR0131063B1 (ko) 1998-04-11

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