KR930017918A - 세팔로스포린 c 아실라제 - Google Patents

세팔로스포린 c 아실라제 Download PDF

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KR930017918A
KR930017918A KR1019930002730A KR930002730A KR930017918A KR 930017918 A KR930017918 A KR 930017918A KR 1019930002730 A KR1019930002730 A KR 1019930002730A KR 930002730 A KR930002730 A KR 930002730A KR 930017918 A KR930017918 A KR 930017918A
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amino acid
acylase
ala
native
acid sequence
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KR1019930002730A
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KR100276541B1 (ko
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미네오 니와
요시마사 사이또
히또시 사사끼
요시노리 이시이
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후지야마 아끼라
후지사와 야꾸힝 고교 가부시끼가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

본 발명은 단백질 공학에 의해 생산된 신규의 변형 세팔로스프린 C 아실라제(CC아실라제), 이것을 코딩하는 DNA, 이 DNA를 함유한 발현 벡터, 이것을 사용하여 형질전환시킨 미생물 및 이 형질전환제를 배양하는 CC아실라제의 생산 방법에 관한 것이다.

Description

세팔로스포린 C 아실라제
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 플라스미드 pYSC305S 및 pCK305중의 변형 CC아실라제 C305S의 전구체를 코딩하는 DNA의 뉴클레오티드 서열 및 추정되는 아미노산 서열을 나타낸 도면, 제2도는 플라스미드 플라스미드 pCK305중의 변형 CC아실라제 C305S의 전구체를 코딩하는 DNA의 뉴클레오티드 서열 및 추정되는 아미노산 서열을 나타낸 도면, 제3도는 프라스미드 pCK269Y중의 변형 CC 아실라제 M269Y의 전구체를 코딩하는 DNA의 뉴클레오티드 서열 및 추정되는 아미노산 서열을 나타낸 도면.

Claims (7)

  1. 숙주 세포중에서 α-서브유니트 및 β-서브유니트로 처리되기 전의 전구체로서 하기 식으로 나타낸 변형 세팔로스프린 C 아실라제.
    A1-268-X1-Tyr-X2-A272-304-X3-A306-773
    여기서, A1-265은 천연 CC 아실라제의 Thr1내지 Gly268에서와 동일한 아미노산 서열이고, A272-304는 천연 CC 아실라제의 Gln272내지 Tyr304에서와 동일한 아미노산 서열이고, A306-773은 천연 CC 아실라제의 Val306내지 Ala773에서와 동일한 아미노산 서열이고, X1은 Met 또는 다른 아미노산이고, X2는 Ala 또는 다른 아미노산이고, X3은 Cys 또는 다른 아미노산이고, 단 X1이 Met이고 X3은 Cys 이외의 아미노산이다.
  2. 제1항에 있어서, X1이 Tyr이고, X2가 Ala이고, X3이 Ser인 변형 세팔로스포린 C 아실라제.
  3. 제1항의 세팔로스포린 C 아실라제를 코딩하는 DNA.
  4. 제3항의 DNA로 이루어진 발현 벡터.
  5. 제4항의 발현 벡터에 의해 형질전환된 숙주 세포.
  6. 제5항의 숙주 세포를 수정 영양 배지 중에서 배양하고, 배양 브로스로부터 변형 세팔로스포린 C아실라제를 회수하는 것으로 이루어지는, 변형 세팔로스포린 C 아실라제의 제조 방법.
  7. 하기 일반식(Ⅱ)의 화합물 또는 그의 염을 제5항의 형질전환체의 배양 브로스 또는 그의 처리 물질과 접촉시키는 것으로 이루어지는, 하기 일반식(Ⅰ)의 화합물의 제조 방법.
    상기 식 중, R1은 아세톡시, 히드록시 또는 수소이고, R2는 카르복실 아실이다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930002730A 1992-02-27 1993-02-26 세팔로스포린 c 아실라제 KR100276541B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929204439A GB9204439D0 (en) 1992-02-27 1992-02-27 A new cephalosporin c acylase
GB9204439.5 1992-02-27

Publications (2)

Publication Number Publication Date
KR930017918A true KR930017918A (ko) 1993-09-20
KR100276541B1 KR100276541B1 (ko) 2000-12-15

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Country Status (15)

Country Link
US (1) US5336613A (ko)
EP (1) EP0558241B1 (ko)
JP (1) JPH0614777A (ko)
KR (1) KR100276541B1 (ko)
CN (1) CN1062903C (ko)
AT (1) ATE121784T1 (ko)
CA (1) CA2090571A1 (ko)
DE (1) DE69300123T2 (ko)
DK (1) DK0558241T3 (ko)
ES (1) ES2071527T3 (ko)
GB (1) GB9204439D0 (ko)
GR (1) GR3015943T3 (ko)
HU (1) HU220460B1 (ko)
TR (1) TR27016A (ko)
TW (1) TW274565B (ko)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT97409B (pt) * 1990-04-18 1998-08-31 Gist Brocades Nv Processo para a preparacao de mutantes beta-lactama-acilases por expressao genetica
ZA932809B (en) * 1992-04-24 1994-10-21 Lilly Co Eli Process for preparing cephalosporins.
TW400384B (en) * 1993-11-01 2000-08-01 Fujisawa Pharmaceutical Co A new cephalosporin C acylase
ATE189477T1 (de) * 1994-08-12 2000-02-15 Dsm Nv Mutierte penicillin-g-acylase gene
CN1065278C (zh) * 1994-08-27 2001-05-02 中国科学院上海植物生理研究所 一步两酶法制造7－氨基头孢烷酸
GB9423212D0 (en) * 1994-11-17 1995-01-04 Glaxo Group Ltd Industrial enzymes
US5821049A (en) * 1996-04-05 1998-10-13 Viratest Carcinogen Monitoring, Ltd In vitro assay for identification of carcinogens
US6153429A (en) * 1996-04-05 2000-11-28 Vera Genics Ltd. Cell lines useful for in vitro assay for indentification of carcinogens
IL129055A (en) 1996-11-05 2005-06-19 Bristol Myers Squibb Co Mutant penicillin g acylases
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
ES2571865T3 (es) * 2005-12-28 2016-05-27 Dsm Sinochem Pharm Nl Bv Acilasas de beta-lactama de tipo II mutantes
CN109913436B (zh) * 2017-12-12 2023-05-23 石药集团圣雪葡萄糖有限责任公司 含有一个或几个点突变的头孢菌素c酰化酶突变体及其制备方法
CN112662655B (zh) * 2020-12-29 2022-05-03 山东金城柯瑞化学有限公司 头孢菌素c酰化酶突变体及其制备方法和应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4959314A (en) * 1984-11-09 1990-09-25 Cetus Corporation Cysteine-depleted muteins of biologically active proteins
JPS61152286A (ja) * 1984-12-27 1986-07-10 Asahi Chem Ind Co Ltd セフアロスポリンｃアシラ−ゼ産生遺伝情報を担うｄｎａ断片
US4752585A (en) * 1985-12-17 1988-06-21 Cetus Corporation Oxidation-resistant muteins
US4835260A (en) * 1987-03-20 1989-05-30 Genetics Institute, Inc. Erythropoietin composition
IL87276A (en) 1987-08-03 1995-07-31 Fujisawa Pharmaceutical Co Analog of a tissue plasminogen activator containing the Pringle 2 and protease regions only, DNA encoding it, processes for its preparation, and pharmaceutical preparations containing it
GB9019724D0 (en) * 1990-09-10 1990-10-24 Fujisawa Pharmaceutical Co Cephalosporin c acylase

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Publication number Publication date
ES2071527T3 (es) 1995-06-16
HU220460B1 (hu) 2002-02-28
EP0558241A3 (en) 1993-12-01
JPH0614777A (ja) 1994-01-25
CA2090571A1 (en) 1993-08-28
GB9204439D0 (en) 1992-04-15
EP0558241A2 (en) 1993-09-01
GR3015943T3 (en) 1995-07-31
EP0558241B1 (en) 1995-04-26
DE69300123T2 (de) 1996-03-28
DK0558241T3 (da) 1995-07-03
TR27016A (tr) 1994-09-15
TW274565B (ko) 1996-04-21
US5336613A (en) 1994-08-09
HU9300557D0 (en) 1993-05-28
HUT65664A (en) 1994-07-28
CN1062903C (zh) 2001-03-07
ATE121784T1 (de) 1995-05-15
KR100276541B1 (ko) 2000-12-15
CN1085252A (zh) 1994-04-13
DE69300123D1 (de) 1995-06-01

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