JPS6480295A - Beta-isopropylmalate dehydrogenase gene promoter and use thereof - Google Patents
Beta-isopropylmalate dehydrogenase gene promoter and use thereofInfo
- Publication number
- JPS6480295A JPS6480295A JP535088A JP535088A JPS6480295A JP S6480295 A JPS6480295 A JP S6480295A JP 535088 A JP535088 A JP 535088A JP 535088 A JP535088 A JP 535088A JP S6480295 A JPS6480295 A JP S6480295A
- Authority
- JP
- Japan
- Prior art keywords
- beta
- gene
- isopropylmalate dehydrogenase
- acremonium chrysogenum
- dehydrogenase gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To obtain a DNA fragment, having a promoter active part of beta- isopropylmalate dehydrogenase gene and capable of more effectively expressing heterogene in microbical cells of Acremonium chrysogenum. CONSTITUTION:A DNA containing gene of beta-isopropylmalate dehydrogenase (IPMDH) which is one of leucine biosynthetic enzymes is cloned from chromosomic DNA of Acremonium chrysogenum to analyze the structure of the gene. As a result, it is found that a region containing a promoter of IPMDH gene and translation starting part can be utilized for constructing useful expression vectors using Acremonium chrysogenum as a host.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP535088A JPS6480295A (en) | 1987-01-13 | 1988-01-12 | Beta-isopropylmalate dehydrogenase gene promoter and use thereof |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP724387 | 1987-01-13 | ||
JP535088A JPS6480295A (en) | 1987-01-13 | 1988-01-12 | Beta-isopropylmalate dehydrogenase gene promoter and use thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6480295A true JPS6480295A (en) | 1989-03-27 |
Family
ID=26339273
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP535088A Pending JPS6480295A (en) | 1987-01-13 | 1988-01-12 | Beta-isopropylmalate dehydrogenase gene promoter and use thereof |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6480295A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998037203A1 (en) * | 1997-02-22 | 1998-08-27 | Agrevo Uk Limited | Fungicidal test method |
WO2000011189A1 (en) * | 1998-08-21 | 2000-03-02 | Aventis Cropscience Uk Limited | Use of leu a promoter of stagonospora nodorum in screening method for fungicides |
-
1988
- 1988-01-12 JP JP535088A patent/JPS6480295A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998037203A1 (en) * | 1997-02-22 | 1998-08-27 | Agrevo Uk Limited | Fungicidal test method |
WO2000011189A1 (en) * | 1998-08-21 | 2000-03-02 | Aventis Cropscience Uk Limited | Use of leu a promoter of stagonospora nodorum in screening method for fungicides |
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